LOCUS       sequence-390511-        2050 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 1 module 6 with BsaI expansion point.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Chamness JC, Kumar J, Cruz AJ, Rhuby E, Holum MJ, Cody JP, Tibebu R,
            Gamo ME, Starker CG, Zhang F, Voytas DF
  TITLE     An extensible vector toolkit and parts library for advanced 
            engineering of plant genomes.
  JOURNAL   Plant Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312.
  PUBMED    36896468
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312."
COMMENT     Sequence Label: pJMC-6-BsaI
FEATURES             Location/Qualifiers
     source          1..2050
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      156..700
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     602..621
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     817..834
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        1085..1189
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgtcagacg aagggaaata aatagtagcc cgcctgatgt gcgggctttt ttttggtggt
       61 ggttaccagt gtcgcttagc taccgtaggt cctcttaggc ggtccgatta gcgacatagt
      121 catcaatgcc ttgacggctt aataagatga tcttcttgag atcgttttgg tctgcgcgta
      181 atctcttgct ctgaaaacga aaaaaccgcc ttgcagggcg gttttttgaa ggttctctga
      241 gctaccaact ctttgaaccg aggtaactgg cttggaggag cgcagtcacc aaaacttgtc
      301 ctttcagttt agccttaacc ggcgcatgac ttcaagacta actcctctaa atcaattacc
      361 agtggctgct gccagtggtg cttttgcatg tctttccggg ttggactcaa gacgatagtt
      421 accggataag gcgcagcggt cggactgaac ggggggttcg tgcatacagt ccagcttgga
      481 gcgaactgcc tacccggaac tgagtgtcag gcgtggaatg agacaaacgc ggccataaca
      541 gcggaatgac accggtaaac cgaaaggcag gaacaggaga gcgcacgagg gagccgccag
      601 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccaccactga tttgagcgtc
      661 agatttcgtg atgcttgtca gggggcggag cctatggaaa aacggctttg ccgcggccct
      721 ctcacttccc tgttaagtat cttcctggca tcttccagga aatctccgcc ccgttcgtaa
      781 gccatttccg ctcgccgcag tcgaacgacc gagcgtagcg agtcagtgag cgaggaagcg
      841 gaatatatcc tgtatcacat attctgctga cgcaccggtg cagccttttt tctcctgcca
      901 catgaagcac ttcactgaca ccctcatcag tgccaacata gtaagccagt atacactccg
      961 ctagcagcgc ccacaggcac ctgcttttcg gaaccgtgag accaacaaca aggtctctag
     1021 tgctgctttt gcaggtgctc gatgaaaagc cttagggtca ggtggcactt ttcggggaaa
     1081 tgtgcgatga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctaat
     1141 gagacaataa ccctgataaa tgcttcaatt atattgaaaa aggaagagta tgagtatcca
     1201 acatttccgt gtcgccctta ttcccttttt tgcggccttc tgccttcctg tttttgctca
     1261 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgccc gagtgggtta
     1321 cattgaactg gatctcaaca gcggcaaaat cttagagagt tttcgccccg aagaacgctt
     1381 cccaatgatg agcactttca aagttctgct atgtggcgcg gtgttatccc gtattgatgc
     1441 tgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtattc
     1501 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gtagtgctgc
     1561 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgattg gaggaccgaa
     1621 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     1681 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     1741 ggcaacaact ctccgcaaac tattaactgg cgaactactt actcttgctt cacgccagca
     1801 actcattgac tggatggagg cggacaaagt tgcaggacca cttctgcgct cggcacttcc
     1861 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     1921 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     1981 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     2041 gcattggtaa
//
