LOCUS       sequence-390526-        6034 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Genetically encoded voltage indicator (GEVI) JEDI-2P in AAV 
            production vector for astrocytes specific expression using the 
            promoter GFAP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6034)
  AUTHORS   Liu Z, Lu X, Villette V, Gou Y, Colbert KL, Lai S, Guan S, Land MA, 
            Lee J, Assefa T, Zollinger DR, Korympidou MM, Vlasits AL, Pang MM, 
            Su S, Cai C, Froudarakis E, Zhou N, Patel SS, Smith CL, Ayon A, 
            Bizouard P, Bradley J, Franke K, Clandinin TR, Giovannucci A, Tolias
            AS, Reimer J, Dieudonne S, St-Pierre F
  TITLE     Sustained deep-tissue voltage recording using a fast indicator 
            evolved for two-photon microscopy.
  JOURNAL   Cell. 2022 Aug 16. pii: S0092-8674(22)00916-3. doi: 
            10.1016/j.cell.2022.07.013.
  PUBMED    35985322
REFERENCE   2  (bases 1 to 6034)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2022 
            Aug 16. pii: S0092-8674(22)00916-3. doi: 
            10.1016/j.cell.2022.07.013."
COMMENT     Sequence Label: pAAV-GFAP-JEDI-2P-WPRE
FEATURES             Location/Qualifiers
     source          1..6034
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   61..190
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     misc_feature    2221..2809
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2274..2294)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2692..2703)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    2841..3317
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3040..3059)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   3357..3497
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3357..3486
                     /label=AAV2 ITR
     rep_origin      3572..4027
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3659..3678)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3869..3890
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4044..4063)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4163..4185
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4223..4241)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4309..4413
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4414..4482,4483..5274)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5445..6033
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5934..5953
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
       61 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
      121 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      181 aggggttcct gcggccgcac gcgtgtgtct agagatctaa catatcctgg tgtggagtag
      241 gggacgctgc tctgacagag gctcgggggc ctgagctggc tctgtgagct ggggaggagg
      301 cagacagcca ggccttgtct gcaagcagac ctggcagcat tgggctggcc gccccccagg
      361 gcctcctctt catgcccagt gaatgactca ccttggcaca gacacaatgt tcggggtggg
      421 cacagtgcct gcttcccgcc gcaccccagc ccccctcaaa tgccttccga gaagcccatt
      481 gagcaggggg cttgcattgc accccagcct gacagcctgg catcttggga taaaagcagc
      541 acagccccct aggggctgcc cttgctgtgt ggcgccaccg gcggtggaga acaaggctct
      601 attcagcctg tgcccaggaa aggggatcag gggatgccca ggcatggaca gtgggtggca
      661 gggggggaga ggagggctgt ctgcttccca gaagtccaag gacacaaatg ggtgagggga
      721 gagctctccc catagctggg ctgcggccca accccacccc ctcaggctat gccagggggt
      781 gttgccaggg gcacccgggc atcgccagtc tagcccactc cttcataaag ccctcgcatc
      841 ccaggagcga gcagagccag agcaggttgg agaggagacg catcacctcc gctgctcgcg
      901 gggtctagag gatccatgga gacgactgtg aggtatgaac aggggtcaga gctcactaaa
      961 acttcgagct ctccaacagc agatgagccc acgataaaga ttgatgatgg tcgtgatgag
     1021 ggtaatgaac aagacagctg ttccaatacc attaggagaa aaatttcccc gtttgtgatg
     1081 tcatttggat tcagagtatt tggagttgtg cttatcattg tagacatcat agtggtgatt
     1141 gtggatctgg ccatcagtga gaagaaaaga ggcattagag agattcttga aggtgtttcc
     1201 ctggctatag cactcttctt ccttgttgat gttctcatga gagtgtttgt tgaaggcttc
     1261 aagaactatt tccggtccaa actggttact ttggatgcag tcatagtagt gggcactctg
     1321 ctaattaata tgacctactc cttctctgac cttgctgcct ttaacagcga gaacgtgtat
     1381 attaccgcgg ataaacagaa aaacggcatt aaagcgaact ttaccgtgcg ccataacgtg
     1441 gaagatggca gcgtgcagct ggcggatcat tatcagcaga acaccccgat tggcgatggc
     1501 ccggtgctgc tgccggataa ccattatctg agccaccaga ccgtgctgag caaagatccg
     1561 aacgaaaaac gcgatcacat ggtgctgctg gaatttgtga ccgcagcggg cattacacac
     1621 ggcatggatg aactgtatgg cggcaccggc ggcagcgcga gccagggcga agaactgttt
     1681 accggcgtgg tgccgattct ggtggaactg gatggcgatg tgaacggcca taaatttagc
     1741 gtgcgcggcg aaggcgaagg cgatgcgacc attggcaaac tgaccctgaa atttatttgc
     1801 accaccggca aactaccggt gccgtggccg accctggtga ccaccttaac ctatggcgtg
     1861 cagtgcttta gccgctatcc ggatcatatg aaacgccatg atttttttaa aagcgcgatg
     1921 ccggaaggct atgtgcagga acgcaccatt agctttaaag atgatggcaa atataaaacc
     1981 cgcgcggtgg tgaaatttga aggcgatacc ctggtgaacc gcattgaact gaaaggcacc
     2041 gattttaaag aagatggcaa cattctgggg cataaactgg aatatgatac agatcagatg
     2101 ccgcacatgg ttactcttct tcgagttctg aaaattgtta tcttaataag aatatttcgc
     2161 ctggcttcac agaagaaaca acttgaagtg gtaacctaat gagatatcaa gcttatcgat
     2221 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     2281 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     2341 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     2401 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     2461 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     2521 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     2581 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc
     2641 gcctatgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     2701 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     2761 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca tcgataccga
     2821 gcgctgctcg agagatctac gggtggcatc cctgtgaccc ctccccagtg cctctcctgg
     2881 ccctggaagt tgccactcca gtgcccacca gccttgtcct aataaaatta agttgcatca
     2941 ttttgtctga ctaggtgtcc ttctataata ttatggggtg gaggggggtg gtatggagca
     3001 aggggcaagt tgggaagaca acctgtaggg cctgcggggt ctattgggaa ccaagctgga
     3061 gtgcagtggc acaatcttgg ctcactgcaa tctccgcctc ctgggttcaa gcgattctcc
     3121 tgcctcagcc tcccgagttg ttgggattcc aggcatgcat gaccaggctc agctaatttt
     3181 tgtttttttg gtagagacgg ggtttcacca tattggccag gctggtctcc aactcctaat
     3241 ctcaggtgat ctacccacct tggcctccca aattgctggg attacaggcg tgaaccactg
     3301 ctcccttccc tgtccttctg attttgtagg taaccacgtg cggaccgagc ggccgcagga
     3361 acccctagtg atggagttgg ccactccctc tctgcgcgct cgctcgctca ctgaggccgg
     3421 gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc
     3481 gcgcagctgc ctgcaggggc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
     3541 ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc attaagcgcg
     3601 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgcctt agcgcccgct
     3661 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     3721 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     3781 cttgatttgg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     3841 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     3901 aactctatct cgggctattc ttttgattta taagggattt tgccgatttc ggtctattgg
     3961 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt
     4021 acaattttat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc
     4081 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
     4141 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
     4201 ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
     4261 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     4321 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     4381 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     4441 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     4501 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     4561 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     4621 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     4681 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     4741 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     4801 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     4861 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     4921 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     4981 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     5041 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     5101 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     5161 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     5221 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     5281 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     5341 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     5401 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     5461 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     5521 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     5581 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     5641 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     5701 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     5761 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     5821 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     5881 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     5941 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     6001 tgatgctcgt caggggggcg gagcctatgg aaaa
//
