LOCUS       sequence-390534-        4245 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor Vector containing ZNF165 transcription factor, part of the 
            Human TFome Collection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4245)
  AUTHORS   Ng AHM, Khoshakhlagh P, Rojo Arias JE, Pasquini G, Wang K, Swiersy 
            A, Shipman SL, Appleton E, Kiaee K, Kohman RE, Vernet A, Dysart M, 
            Leeper K, Saylor W, Huang JY, Graveline A, Taipale J, Hill DE, Vidal
            M, Melero-Martin JM, Busskamp V, Church GM
  TITLE     A comprehensive library of human transcription factors for cell fate
            engineering.
  JOURNAL   Nat Biotechnol. 2021 Apr;39(4):510-519. doi: 
            10.1038/s41587-020-0742-6. Epub 2020 Nov 30.
  PUBMED    33257861
REFERENCE   2  (bases 1 to 4245)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0742-6"; journalName: "Nat Biotechnol"; date: 
            "2021-04"; volume: "39"; issue: "4"; pages: "510-519"
COMMENT     Sequence Label: pDONR223-ZNF165
FEATURES             Location/Qualifiers
     source          1..4245
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1556..1655)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1672..1691)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1673..1691)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1696..1712)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1696..1712)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2143..2934
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3027..3615
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3516..3535
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3769..3786
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3945..3972)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4064..4150)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4199..4221
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4213..4230
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4214..4230
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagttggca tggctacaga accaaagaaa
      121 gctgcagccc agaactctcc agaggatgaa ggacttctga tagtgaagat agaagaggaa
      181 gaatttatcc atgggcagga cacttgctta cagagaagtg aactccttaa gcaggagctc
      241 tgcaggcagc tttttaggca gttctgctac caggattctc ctggacctcg cgaggcactg
      301 agccgcctcc gggagctctg ctgtcagtgg ctgaagccag agatccatac caaggaacag
      361 attctggaac tgctggtgct agagcagttc ctgaccatcc tgccaggaga tttgcaggcc
      421 tgggtacatg aacattaccc agagagtgga gaggaggcag tgaccatact agaagatttg
      481 gagagaggca ctgatgaagc agtactccag gttcaagccc atgaacatgg acaagaaata
      541 ttccagaaaa aagtgtcacc tcctggacca gcacttaatg tcaagttaca gccagtggag
      601 accaaggccc attttgattc atcagaaccc cagctcctat gggactgtga taatgagagt
      661 gaaaacagta gatccatgcc aaagctggaa atttttgaaa aaattgaatc acagagaatt
      721 atatctggaa gaatctcagg atacatatca gaagcgtctg gtgagtctca agacatctgt
      781 aagtctgcag gcagggtaaa gagacaatgg gaaaaagaat caggggagtc tcagagactc
      841 tcgtctgccc aggatgaagg ttttggtaaa atcctcaccc acaaaaatac agtcagaggt
      901 gaaataataa gccacgatgg atgtgagagg agattaaatc tgaactcaaa tgaattcaca
      961 caccagaaat cttgtaaaca tggtacctgt gaccagagct tcaaatggaa ctcagatttt
     1021 attaaccatc aaataattta tgctggagaa aaaaatcacc aatatggaaa atctttcaag
     1081 agcccaaaac ttgctaaaca tgcagcagtt ttcagtggag ataaaactca tcagtgtaat
     1141 gaatgtggga aagctttcag gcacagctca aaacttgcta ggcatcagag aatccacact
     1201 ggagagagat gctatgaatg taatgaatgt gggaaaagct ttgcagagag ctcagatctt
     1261 actagacatc ggcgaattca cactggggaa agaccctttg gttgcaaaga atgtgggaga
     1321 gcattcaacc tgaactcaca tcttatcagg catcagagaa ttcacaccag agagaaaccc
     1381 tacgagtgta gtgaatgtgg gaaaaccttc cgagtgagct cacatcttat tcgacacttt
     1441 agaattcaca ctggagaaaa accctatgaa tgcagtgagt gtggaagagc cttcagtcag
     1501 agctcaaacc ttagtcaaca ccagagaatt cacatgaggg aaaacctatt aatgtaccca
     1561 actttcttgt acaaagttgg cattataaga aagcattgct tatcaatttg ttgcaacgaa
     1621 caggtcacta tcagtcaaaa taaaatcatt atttgccatc cagctgatat cccctatagt
     1681 gagtcgtatt acatggtcat agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc
     1741 tgatgttaca ttgcacaaga taaaaatata tcatcatgcc tcctctagac cagccaggac
     1801 agaaatgcct cgacttcgct gctacccaag gttgccgggt gacgcacacc gtggaaacgg
     1861 atgaaggcac gaacccagtg gacataagcc tgttcggttc gtaagctgta atgcaagtag
     1921 cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg taacggcgca
     1981 gtggcggttt tcatggcttg ttatgactgt ttttttgggg tacagtctat gcctcgggca
     2041 tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga tgttatggag cagcaacgat
     2101 gttacgcagc agggcagtcg ccctaaaaca aagttaaaca ttatgaggga agcggtgatc
     2161 gccgaagtat cgactcaact atcagaggta gttggcgtca tcgagcgcca tctcgaaccg
     2221 acgttgctgg ccgtacattt gtacggctcc gcagtggatg gcggcctgaa gccacacagt
     2281 gatattgatt tgctggttac ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg
     2341 atcaacgacc ttttggaaac ttcggcttcc cctggagaga gcgagattct ccgcgctgta
     2401 gaagtcacca ttgttgtgca cgacgacatc attccgtggc gttatccagc taagcgcgaa
     2461 ctgcaatttg gagaatggca gcgcaatgac attcttgcag gtatcttcga gccagccacg
     2521 atcgacattg atctggctat cttgctgaca aaagcaagag aacatagcgt tgccttggta
     2581 ggtccagcgg cggaggaact ctttgatccg gttcctgaac aggatctatt tgaggcgcta
     2641 aatgaaacct taacgctatg gaactcgccg cccgactggg ctggcgatga gcgaaatgta
     2701 gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat
     2761 gtcgctgccg actgggcaat ggagcgcctg ccggcccagt atcagcccgt catacttgaa
     2821 gctagacagg cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg
     2881 gaagaatttg tccactacgt gaaaggcgag atcaccaagg tagtcggcaa ataaccctcg
     2941 agccacccat gaccaaaatc ccttaacgtg agttacgcgt cgttccactg agcgtcagac
     3001 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     3061 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     3121 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     3181 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     3241 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     3301 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     3361 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     3421 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     3481 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     3541 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     3601 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     3661 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     3721 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     3781 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     3841 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
     3901 attaatacgc gtaccgctag ccaggaagag tttgtagaaa cgcaaaaagg ccatccgtca
     3961 ggatggcctt ctgcttagtt tgatgcctgg cagtttatgg cgggcgtcct gcccgccacc
     4021 ctccgggccg ttgcttcaca acgttcaaat ccgctcccgg cggatttgtc ctactcagga
     4081 gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtcttccgac tgagcctttc
     4141 gttttatttg atgcctggca gttccctact ctcgcgttaa cgctagcatg gatgttttcc
     4201 cagtcacgac gttgtaaaac gacggccagt cttaagctcg ggccc
//
