LOCUS       sequence-39054-e         736 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV expression of mammalian codon optimized ChIEF for optogenetic 
            excitation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Jurgens CW, Bell KA, McQuiston AR, Guido W
  TITLE     Optogenetic stimulation of the corticothalamic pathway affects relay
            cells and GABAergic neurons differently in the mouse visual 
            thalamus.
  JOURNAL   PLoS One. 2012;7(9):e45717. doi: 10.1371/journal.pone.0045717. Epub 
            2012 Sep 20.
  PUBMED    23029198
REFERENCE   2  (bases 1 to 736)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(9):e45717. doi"; pages: " 
            10.1371/journal.pone.0045717. Epub 2012 Sep 20"
COMMENT     Sequence Label: pACAGW-oChiEFtdTomato-AAV
FEATURES             Location/Qualifiers
     source          1..736
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(77..96)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     intron          79..211
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     complement(311..334)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(509..528)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 attttgtaat ccagaggttg attatcgata agcttgatat cgaattcgaa tcgactagtc
       61 gcgagctcca ctcgacacct gtggagagaa aggcaaagtg gatgtcagta agaccaatag
      121 gtgcctatca gaaacgcaag agtcttctct gtctcgacaa gcccagtttc tattggtctc
      181 cttaaacctg tcttgtaacc ttgatactta cctgcggcct cgagctactt gtacagctcg
      241 tccatgccgt acaggaacag gtggtggcgg ccctcggagc gctcgtactg ttccacgatg
      301 gtgtagtcct cgttgtggga ggtgatgtcc agcttggtgt ccacgtagta gtagccgggc
      361 agttgcacgg gcttcttggc catgtagatg gtcttgaact ccaccaggta gtggccgccg
      421 tccttcagct tcagggcctg gtggatctcg cccttcagca cgccgtcgcg ggggtacagg
      481 cgctcggtgg aggcctccca gcccatggtc ttcttctgca ttacggggcc gtcggggggg
      541 aagttggtgc cgcgcatctt caccttgtag atcagcgtgc cgtcctgcag ggaggagtcc
      601 tgggtcacgg tcaccagacc gccgtcctcg aagttcatca cgcgctccca cttgaagccc
      661 tcggggaagg acagcttctt gtaatcgggg atgtcggcgg ggtgcttcac gtacgccttg
      721 gagccgtaca tgaact
//
