LOCUS       sequence-390540-       19554 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian cell expression of wt Strep-BIRC6.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 19554)
  AUTHORS   Hunkeler M, Jin CY, Fischer ES
  TITLE     Structures of BIRC6-client complexes provide a mechanism of 
            SMAC-mediated release of caspases.
  JOURNAL   Science. 2023 Mar 17;379(6637):1105-1111. doi: 
            10.1126/science.ade5750. Epub 2023 Feb 9.
  PUBMED    36758104
REFERENCE   2  (bases 1 to 19554)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.ade5750"; journalName: "Science"; date: 
            "2023-03-17- 17"; volume: "379"; issue: "6637"; pages: "1105-1111"
COMMENT     Sequence Label: pDARMO_CMVT_N_Strep_BIRC6
FEATURES             Location/Qualifiers
     source          1..19554
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(391..594)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(420..440)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        595..898
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     mobile_element  992..1157
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     promoter        1182..1253
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1254..1322,1323..2114)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1472..1491)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2153..2741
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2642..2661
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     mobile_element  2805..3028
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(3095..3628)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(3817..3822,3823..3839,3840..3845))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     polyA_signal    3893..4100
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     4089..4106
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             4173..4184
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     4582..4602
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(19288..19311)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             complement(19333..19356)
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     regulatory      19362..19371
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(19424..19443)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
ORIGIN
        1 agagggagta ctcaccccaa cagctggatc tcaagcctgc cacacctcac ctcgaccatc
       61 cgcggtgtca acaccgccta ctttaattac atcatcagca gcacctccgc cagaaacaac
      121 cccgaccgcc acccgctgcc gcccgccacg gtgctcagcc taccttgcga ctgtgactgg
      181 ttagacgcct ttctcgagag gttttccgat ccggtcgatg cggactcgct caggtccctc
      241 ggtggcggag taccgttcgg aggccgacgg gtttccgatc caagagtact ggaaagaccg
      301 cgaagagttt gtcctcaacc gcgagcccaa cagctggccc tcgcagacag cgaggcggaa
      361 gagagtgagg atctgacggt tcactaaacc agctctgctt atatagacct cccaccgtac
      421 acgcctaccg cccatttgcg tcaatggggc ggagttgtta cgacattttg gaaagtcccg
      481 ttgattttgg tgccaaaaca aactcccatt gacgtcaatg gggtggagac ttggaaatcc
      541 ccgtgagtca aaccgctatc cacgcccatt gatgtactgc caaaaccgca tcaccatggt
      601 aatagcaatg actaatacgt agatgtactg ccaagtagga aagtcccata aggtcatgta
      661 ctgggcataa tgccaggcgg gccatttacc gtcattgacg tcaatagggg gcgtacttgg
      721 catatgatac acttgatgta ctgccaagtg ggcagtttac cgtaaatact ccacccattg
      781 acgtcaatgg aaagtcccta ttggcgttac tatgggaaca tacgtcatta ttgacgtcaa
      841 tgggcggggg tcgttgggcg gtcagccagg cgggccattt accgtaagtt atgtaacgcg
      901 gaactccata tatgggctat gaactaatga ccccgtaatt gattactatt aataactagc
      961 tagcttaagg gcgaatccgg accgcaggct gaaccagata agtgaaatct agttccaaac
     1021 tattttgtca tttttaattt tcgtattagc ttacgacgct acacccagtt cccatctatt
     1081 ttgtcactct tccctaaata atccttaaaa actccatttc cacccctccc agttcccaac
     1141 tattttgtcc gcccacaacc ggttgacttg ggtcctaaat acattcaaat atgtatccgc
     1201 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     1261 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     1321 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     1381 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     1441 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     1501 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     1561 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     1621 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     1681 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     1741 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     1801 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     1861 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     1921 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tcacgcggta
     1981 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     2041 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     2101 ttaagcattg gtaataagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     2161 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     2221 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     2281 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     2341 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     2401 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     2461 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     2521 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     2581 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     2641 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     2701 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     2761 ttttacggct tcctgtggat gcgcagatgc cctgcgtaag cgggtgtggg cggacaataa
     2821 agtcttaaac tgaacaaaat agatctaaac tatgacaata aagtcttaaa ctagacagaa
     2881 tagttgtaaa ctgaaatcag tccagttatg ctgtgaaaaa gcatactgga cttttgttat
     2941 ggctaaagca aactcttcat tttctgaagt gcaaattgcc cgtcgtatta aagaggggcg
     3001 tggccaaggg catgtaaaga ctatattcgc ggcgttgtga caatttaccg aacaactccg
     3061 cggccgggaa gccgatctcg gcttgaacga attgttaggt ggcggtactt gggtcgatat
     3121 caaagtgcat cacttcttcc cgtatgccca actttgtata gagagccact gcgggatcgt
     3181 caccgtaatc tgcttgcacg tagatcacat aagcaccaag cgcgttggcc tcatgcttga
     3241 ggagattgat gagcgcggtg gcaatgccct gcctccggtg ctcgccggag actgcgagat
     3301 catagatata gatctcacta cgcggctgct caaacttggg cagaacgtaa gccgcgagag
     3361 cgccaacaac cgcttcttgg tcgaaggcag caagcgcaat aaatgtctta ctacggagca
     3421 agttcccgag gtaatcggag tccggctgat gttgggagta ggtggctaca tcaccgaact
     3481 cacgaccgaa aagatcaaga gcagcccgca tggatttgac ttggtcaggg ccgagcctac
     3541 atgtacgaat gatgcccata cttgagccac ctaactttgt tttagggcga ctgccctgct
     3601 gcgtaacatc gttgctgctg cgtaacatcg ttgctgctcc ataacatcaa acatcgaccc
     3661 acggcgtaac gcgcttgctg cttggatgcc cgaggcatag actgtacaaa aaaacagtca
     3721 taacaagcca tgaaaaccgc cactgcgccg ttaccaccgc tgcgttcggt caaggttctg
     3781 gaccagttgc gtgagcgcat acgctacttg cattacagtt tacgaaccga acaggcttat
     3841 gtcaacgagt agccaccgtt cgggcgtccc agcgattcgc ccttaactcg agtccccagc
     3901 atgcctgcta ttgtctgccc aatcctcccc cttgctgtcc tgccccaccc caccccccag
     3961 aatagaatga cacctactca gacaatttga tgcaatttcc tcattttatt aggaaaggac
     4021 agtgggagtg gcaccttcca gggtcaagga aggcacgggg gaggggcaaa caacagatgg
     4081 ctggcaacta gaaggcacag tcgaggcaga tctcgaagcc gcggaagagg ccgcagagcc
     4141 ggcagcaggc cgcgggaagg aaggtccgct ggattgaggg ccgaagggac gtagcagaag
     4201 gacgtcccgc gcagaatcca ggtggcaaca caggcgagca gccaaggaaa ggacgatgat
     4261 ttccccgaca acaccacgga attgtcagtg cccaacagcc gagcccctgt ccagcagcgg
     4321 gcaaggcagg cggcactgag ttccgccgtg gcaataggga gggggaaagc gaaagtcccg
     4381 gaaaggagct gacaggtggt ggcaatgccc caaccagtgg gggttgcgtc agcaaacaca
     4441 gtgcacacca cgccacgttg cctgacaacg ggccacaact cctcataaag agacagcaac
     4501 caggatttat acaaggagga gaaaatgaaa gccatacggg aagcaatagc atgatacaaa
     4561 ggcattaaag cagcgtatcc acatagcgta aaaggagcaa catagttaag aataccagtc
     4621 aatctttcac aaattttgta atccagaggt tgattttacg gccacaggcc attacggcca
     4681 caggccatta tcatcagcgg ccgctcaaag ctgaaagtcg gacggaagtt cctttgaact
     4741 tgagggtttt acctgatcgt gcataagggt ctcttccgca cctgtagtgg ctctacatac
     4801 ttctggggcg tcgtccgtgt caggatccaa accctcagga cacggcaatt tcaagagttc
     4861 ctcgcggagt tgggccgtat gtcgtttgag agccgctgcg tgatgagaca tagtccgtcc
     4921 gacccgtttg tctgaggaat attgttggat gtctgctatc cactcttcac actgcgccat
     4981 aatttctact cttttcaaat agaaatgttt atgtatcacc tccttgaagc aaggagaggg
     5041 attcctaatc tgttcgagca tagcccactt cactgtagct tggcggatat tgccatcgta
     5101 ctccctgctg gattgagtac cagatggtgt gcctcgagac ctctcgtaac caggctcgtt
     5161 aaagtaaggt tccgccacga ggatcaatga ttgaacggac acgagtacct ggagaaatga
     5221 tgatgtttgc gggttccatt tttcttcggg tcttccatgc catgtattca gaattgagag
     5281 gcaaactttg ccgtcgttat acaggttggg gttgaagcgt acggagtgac ccccggtagt
     5341 ttccaaattg acaaggggcg ggctggaagg gtaatcctgt gggaagtaaa cgtcaaactc
     5401 gaagcatccg tttgcataag gagtgtctgc ggggccggtg atcagaactt tcataatatc
     5461 caatctctct tcatcacatc tcacgaagac tgaactactg gatgacaagg ggagactagt
     5521 actgagggta accgcttctt gagcaaggcg ccgagcccta gctgcactat ttgcatcgtt
     5581 agcgtttttg acctggctca tataatgata gttcactttg aatcccagct tgccgtcctc
     5641 gtcttcagac accatttcaa aagtgtcgaa ttggagcttt ttcatcactg cgacatactt
     5701 ttcctcaagg gatttcagga cggacagcgg cttaggcttc atggccgctt tctttgaata
     5761 ctccccaagt ttcttctcct ggttggcctg tcggagtgat gttgttgcag cgtaaacaat
     5821 ttcagctgtt ttctgtatat ctggtacgag caaagtaagc ccttcgggtt cctggtcaga
     5881 tgcgtctggc ttaactccgg ttttaacatt ctctctctta gaccgcaggc ggttggtata
     5941 agtatcgaca caagttttca ttttggcaag gagagtgcca acactggtct ggcactcgct
     6001 ctgctcctcc tcctcctcgc cattctcggt gctcaggggg agcaggagag gcaccatggc
     6061 agcgcaactg gctatagctc tcagcagttc cagaagagct ctatacaagg gaacatgtct
     6121 cgccatgtcc agcacagagt cgtttcgaag gtatgaactc atggcaggta tcaaacaact
     6181 ctggctaaga agctccagga gcacactggg gagagcattg gaattctgac cgcgcgtttc
     6241 gtgactggat tgtgcctcgc cattcaccgc tgaagacaca gggtttatat agctcgcaag
     6301 cacctggagc agacaagtta catgttcctc ctccaacctt tgcttcgtaa gagcttgttc
     6361 cacatcccat ccggaggcgg ttgagccagt cccaaatccg gtcccttttg cccagtagag
     6421 ctgctgctcc tcggtactcg tggggttgtg ggagctgctc acctgtggtt ccgtctggtt
     6481 cacgctgctg tttgggaccc ggggactgtg gtggctcaac gcactgaggc acacgagaat
     6541 gaggtgcaag gccccgatct caagggccat tcgtcgcaga aggacgccgt catcagttgt
     6601 gaggggggta gttgaaaaga gggatcggag cacatgaaaa ggaagtgtgg gcaatacgtt
     6661 cgcgctgggg ctctgaagga tatgggaacc ttctccatcg tccgtcaccc ccaaaacgag
     6721 ccgcagaagg cattgcgcat gcttgcgttc tttcagcagg acttcagcgt aaccagggag
     6781 cctaagaaaa agcccaaatg ccgccaaaga gtgcgctggt attggagaca ttgtctggac
     6841 ggcggatttt attgggggcg gctcggcagc aaccgtttcg ggggcctcat acaccaattc
     6901 gccgctctgc tcaattgtca cccattcaaa ttgatctgaa tctttaggtt tctcttgggt
     6961 ggttgaggtt acaaccggag ctgatacctg ctggatcact tcaggataaa gcatgggcaa
     7021 cctctcggcg atgagagcaa gaccacccat cccggcgaat acctggagcg gggattgtat
     7081 cggacaggtt tccagcaggc tttcatccaa aattccgtcc atgcattcgt cgccgggtgt
     7141 caaggcctcg tcctctgggc aggaagcaag gaggtcaccg tgatcctttt cagggtgcaa
     7201 gcgcttatag gaatcagttt tggacagtga agggtctgta atcacccgac tgcccagctt
     7261 aaccgtaaga tctatagatc tgtacccctg aggcaacttg cggtcataaa gcagggtgag
     7321 cagttgagcc agagtcattt ccgctggcag tggctgacct gcgagaagct tatggaacag
     7381 atgaaacact ggtaccgtga tgattttatt cgctgcctcg gcgccgctcg tgcttccgat
     7441 cttgtccggt atcgtcctcc cgcggcgtga tggtggtcga ggaggggtcg cgctgaccgc
     7501 cctcttgctt tgactcttga gcccggaggc gactaccacg gagtagttat cctgaaagcc
     7561 attttcggct ttgactttct ctttttcctc gtggtttttt ttttctttgt catctttctg
     7621 ctcggagata agcttggcgg tgatgctcgg agtatcggag accctatcaa ggacatctga
     7681 aagcgtcgta gatactggca ggtggagtgt tctaaatttg tgcccggccc cgtacatcgg
     7741 gtgctgaatg acatggctcg tggcatttat ccttccctta tagagcgggc acgggctctg
     7801 caggaacata gtgacctttt cgtcctccag catgagttga aggaagagtc gtctgataaa
     7861 accgcttatg ttgccagacg tcgggagatt gcccctctgt ggactggtct gaaagagctc
     7921 gcaaagcact tgagccataa gcttttgatt atttgggtgg caacttatgc actggagaaa
     7981 aaacgccact gtcgcgtttt ctatagcggt tctttgttgt gttgtaagac ccgtagtagc
     8041 ggcagaactc aagctggcag acctagcgct cgtctgctgg gccccgagat tgtgtgagcc
     8101 gctggtactc ccagaatgac acaacaagaa cagcagtgca gtccagagag gattgacttc
     8161 ggaaccaccc agccagtctt tcatgatgtg gctgttaccg acctcggtaa ggaaccgcaa
     8221 tatcggggcg acgagatccg ccgtaatcgg cattttgtta cattgctggg gaacatgatg
     8281 ccgccggagt ttatcctgtg aaatgtcaat gctctgtgca atggattcac tactacttat
     8341 gtgggaaaaa cagaatgaag ccaagctccg aacaaggagg ctcggcagcc cgctgtccag
     8401 gagctgctta atagcttcag gagactgtga agaacttgcg agggtagcca agtggctttc
     8461 agtcagcgcc aagggtgctt gggcattttt agaatcatct gtcagagatg aagacaagtc
     8521 ttgctttttg ctgtcatctg tcatggacaa gcggtgatgg cattgaaccg gcgggggggt
     8581 tatgcccatc caacccatca acagagagaa gtagttagga tgtacgtgac acatagagca
     8641 aagcaagctg tcaacagcct ttttgagaac catattacac ggcagagaca tggaccaatt
     8701 aaacagaagc tcaaagagct cctgatccag cagtgctggc aagtcgtatt caacgagcaa
     8761 ctcataggag tgccacagaa ttgccgcaac gcaatgcagg tgactcggtg acggcatcag
     8821 aagcccgtat tctacagtgc tggaattcgg gcacatgtaa ttcattcggc cagatctttt
     8881 catagctgct acgcgcgcgc tatcgcttac ccatttgagc aatgcctgaa tccgatcttt
     8941 ggttcccgga tcttgggtag ttccaagttg atacaaaatg tctattgtac tatcgctaga
     9001 gaggccattc aaccgaccaa acaacagtgg actgttcaga tctgtaggat cagaaccact
     9061 agctgcacaa ttccgcaaca agatgtctat aagcttgaga cctatccgag ttgactggag
     9121 gccgatttta accaagacga cctcaatatt gggtgagtgc attccgcaat agggggacat
     9181 gagaagtgcg gcgcaagtct ggagaagatt ggccgtgggg gcagccgctg aagccatcat
     9241 tccttccaga tctgagatat gcgtgagaca gtgatggagg agccggagcc atccgataga
     9301 tgttttgcta acttgatctt cggaaggcaa aaacggatta tttaccgtag cagaagatgt
     9361 ggtcccaaac gctgtaagcc ccaggagttt aatctgggaa agcccgagcg tggaagcgtc
     9421 gcgggggcgg tgcaagcgca agcagaccgc ggaagctacc tcagctttta caagttggat
     9481 ctttatgtag gtaagtcctg aagttacgac cggagtactg agcgggagca tatttactcc
     9541 atccgcacta acctcgactg atacagagga tggacacgta gccaaagaag ccaggtgagg
     9601 ctgaatatgg atttccttaa ggagcaccgc ggcagggaga tgaatagtga gatcgcacca
     9661 tgcttcctct ggcaagaata tatatgacca agctgcggat ctggccctcc ggtgaggagg
     9721 agtggcttga agcaggactt ccgccggctg tgccgttgga gaagagctcg taattgtacc
     9781 caaaggtgca aaattgtgta ttccttcagc attatctggc tcagaggcaa ggatcctcgt
     9841 cttcaaggta gaatcgactg ccttgttcgg cccagagtca aggaacttca taatggtgga
     9901 gaccatagat cttgctgtgt tgacaatcgc tctagtgctt gtaaccatgt tattgcagat
     9961 gagctgcact cctcccatgt catggaaggc tttaagcgcg tcagaagtat cgaggactcg
    10021 gagtatgaac cacagcagag gctcagacaa ctggcatgtg gcaagtgacc cctgccggcc
    10081 catgtatcca catgccatat atgtcaagat cggctgcagc atcatgtgaa ccgtagacgc
    10141 tttcttgctc aaagtagtca gaatcgtgaa aagtccatct cccacagaga tcgcatccaa
    10201 accgccatga aagttttcat gcttagtcaa atgcaaccca gaagctccta taatcatggc
    10261 cattgcagag gaattgcaca agccgagtgc ggacagaatt tgactcatta tctgttgatt
    10321 ggcgagcacg agatccgcca cgtaggctgg ggatccttgt acgctggtgc tggaaccatt
    10381 tccatcttta cccccggaca ctttttcttc gtcggaaacg gaatccgtcg tattagttgt
    10441 gacgcttact ctagcactgt attccctatt gccactcaaa ggcagttgca caaggatgtt
    10501 gacgagcttc agaagatcga acatcaactg atctctggtc atctctccgc acactgcttt
    10561 tgcgtctcca ggtcggatca gattagcgaa gagaccacca aaacaagccc gagcgcccac
    10621 actactgtct gagccacttc ttgacatgac cttatctgag catgtgatat aatggtgtac
    10681 aaggaacgta acgctttcga tgacagcaga tatagtagag acacttacaa cctcgaagtt
    10741 ttgttcaagg gtgaactcca acagcttgac ttgagcatcc aaaggacctt gccccgtctg
    10801 gaacgcccct ctttcggtac tgaggatatg aataagcttg aggaggaatt ctgagaatat
    10861 ctgagggcag gttgaagaaa ggtgtacctg gagtctggtc aaaaactcct gcatcgcctg
    10921 ggtggctgta ggtccaactt gtgggctgtg ttggttcgta ccatgcgggg aagtaccaga
    10981 gaggaattta accagtacgt ggatcaggtt tggatctgac accaacaggt gtgccgtaga
    11041 ttcctgagtt cctatagcgc tggaactacc tgaattcaat tgcatatttg tcatcagggt
    11101 cagactatgc aaaaccaagc accaggtgcg caaaagtgtg ttgggatacc acgccagcac
    11161 ggtgaggaag ctggtgatag aggcttgatt aagactaatc accggtattc tattggcatt
    11221 gtacagaaag atatctgcac cattttcctt attgcctgta gaggaactat tcagggaaag
    11281 tgtaagccac agttggagca aggactcaag ctgcacgtgg tgaacctgga gcatggagaa
    11341 aagtttgttg aaacaaacgt tcagcatagg tacagatgag agctgtataa tcagctggct
    11401 ggtttgattc gcagcttgaa gcccaccaag gagtgaatca tcggtcccag ttgggctctg
    11461 agacaaggta ggtgacgtat tggtcagagc ctgcaatata ctgtccgctt caagagtgct
    11521 catcattttc aacatcaact ctgcctgctg ctccaaaccc acttccaggc gagcatccag
    11581 ggcttgagaa acggacacgg atacagtctg actaccgttc ttctcagtat tcgctggagg
    11641 tttcgcaact gggattccaa gtccacctat gtaaggatta tagttatatg taccgtagtc
    11701 cgcgccccaa taatcgtacc aagtagatga gatgggtttg aatacctttg ccgccaatgg
    11761 gtccttctcc aggtctatgt cgagtggatc gatgtctact tccataaggt cctggagaag
    11821 ctcgagatct atctctttgt ctttctcaag tgatgacagg gggggtgcac cagtgatgtc
    11881 gtgggtaagg ggttcgtcaa tggtctccag aagctgaact gatgattgtt gcagactatc
    11941 atcggaatcc cccgccgtag ctccaacatc atcaccaacg gtcccttctt ccatcgcttc
    12001 ggcggccacc ggagcaagca actctcctgc gagtatatct tggagcatac aagtcaatga
    12061 gtattgggcc caggcgccgc cccagctgat gtcgcctcgg ttgaagtccg tcccgaccaa
    12121 caagagcagc aatctttcca gttggggttc atttacaatt tcgcacaagt gtattgttgg
    12181 cctggtcgca ttagcaattc gtgccaagac cttacaaacg aagagcaaga ggtcggcatg
    12241 acatgtgaaa tccatagaca acaagaaaag aacaagcttt tgaaccacgc taatgcacct
    12301 ttcatgggcc aaagtgaaag gcagtggttc aatttccgga gactccttgg ccgtggtaac
    12361 ttctgtatca agattacttc tactccactc ggccgtccgt ctcagcctaa tgagatcttt
    12421 aaaatgctta ctcgttgctt gcttaagttt ggcctgctca acgagcaact tatagctaga
    12481 tcccttattt gactgaattt tttctttctc catctgctca accagagcct tttgtttcgc
    12541 cttgaggagg ttaagctgct gcttatgatg aacgagttgt ttccggattt ttcttgagta
    12601 caatctatcc aggctacttg accctttgct agagcggttc aaggattgtg tatgaagatt
    12661 attgtttatg aaagaccact gattgccatt caaaggtttt tttgctgcgg ctgcttcatc
    12721 ctctacggtt gccacatagt caagcaaccg actgaccaac attacgaccc atgatatcat
    12781 tgggatgtca agaacccctt cagtccgtcg gtgcatgggg agctgtggct gcaacgggga
    12841 aagcaaatta tccaagaggt tcagcaggga ttcgagtaca ccgctattag acaagcttcg
    12901 ctgtccaatg cagctgagca acatgaacac ccggtcttgc ggaaatatca aaagttgttc
    12961 agaattataa agctcctgca aaacgttgga aagaaattga ccccaccacc tctccccgcc
    13021 gcacaattgc accagcaaaa gccctgtgtg aagcctaatc tttggtgtcc ctgaaataca
    13081 caaatgcttg aagagttcct cacaagcttg cgcatggaat gtgctctgca ggactgctgg
    13141 cacggaatgt ccattgcttg cgtgcaacaa ggagagaagc gtgtccagca agtaccgtat
    13201 gatcgttcgc agttgttctg tggagcttgt ctgtatcagg tcttcgggat tacttgtttc
    13261 agagagcatc tttcttgagg cccccaaggc aaccttcagg cgttgaacag cattgtgggc
    13321 aaggttgagc tgaagctgaa gttgaataca gtcctggtat gcagagaaga ccctagaatc
    13381 ttcctcgaca gctttgtcgg gtttcctcag gaagtactgc gcgttgttag cgctattcaa
    13441 tggtggaaga tcaatggact ggagcaaggt ttccaagcgg tgacaagcca gattgtagcg
    13501 acactgaatg tcttcttgca atgccaccat catggcaagg tgctgatcaa tctcgggctg
    13561 attggcagat tccccttcgc ctttcaatgg gtcatgcatc aacttaagct cggactccca
    13621 aggaagaatg tacgtgtgtc catagtaaaa accgagtggt atcttcgccc ttgcattcgt
    13681 tgaaccatac cggccgatga ctgtaatttt catgaagcga catacggggg gcggaatgag
    13741 atcatgcaaa atcagggagt gagtgctaat gtctgttgct acaaccagtc tgcgaccgtc
    13801 aacctcttca cccagggtcc atatatcaat ggaaaggcta gccaagtcgc cacaggtggg
    13861 tataagaacg tctgtcagga ggattggcct tccgaaatcc aacgtaacga agcgtctagc
    13921 tccactatgc atccgttcaa ttatgatgga ttgatgggga gggggctgaa ggaagtggct
    13981 agcgtgtgag atggcaagag caagtcctga ccccaaaggg ttcattcggg atttgtttga
    14041 gtttttgttg tgtgctgcga gattgacggc aggcgggtca ataactgaac ccgggaaaag
    14101 ctgggccaac tctgcgttga tgacgacaga aacagcttca tttggcggcg tcaagggtgg
    14161 agtcataaac aggggtgtcg tttttggagt aggcgggatc acatcgcttg ggtgtataaa
    14221 gaagcccggg gcagcgacgg ggttgctagg gacgctattg tgtacggggc cgacagctga
    14281 cgcagctgcg gctgccgctg cggttgtttg gtgcagtttc gcctccaact tcgccttctg
    14341 ttgttgcagc ttaagaagtt gttgctgctt ttcctgcaga acctggagtt gctgctccgc
    14401 actggccatg gcatgggaca gagactgcag agccacctgt gcagcggaac tcagagccgt
    14461 tgatgcctcc cctaccgttc ctgaggagag ccctcccacc gccggtgtca caccgaagct
    14521 actcatagca tcatctcgct caatccgcgt cccgtcagat gttgaaacac atgtgaaatg
    14581 gagcggctcg acttcaagaa gtccggtaag gctagtaaat gagccttcag ccgccgtaca
    14641 acttgaaacc ttaccgtttc cactgagtac gtcggacaaa tctatttcgt cagactggac
    14701 ccctatgctg ctgttatata cattcttgca tgaagaccca gacatctcaa ggtcgaacag
    14761 cactgggtcg aacggagagg agtagagtcc atatcgtgtc tggagaagcg cttccatcgg
    14821 tgtcaagcga tcctgcagtt ggcgggagac agcagtcaaa agtgaggcac aggccgtact
    14881 gcagccagca agatcttcat ccttgacgta gttcagaaga acaaaatacc agtacactga
    14941 gcccccagtc gtggcagccg gcagaaatga catgttatcg aggagggcct tcaaaaggac
    15001 tggaccgaac gcgggttggc aaggatcgca cttaccgttt gagatacaga gcgcgagaaa
    15061 cctagcgcat ttgtgtgcta tacttcgccc ggcttcgaaa aagcagacgc gcacaatatc
    15121 actcaggaat ttccgggttt ttgacaggag attttcgtta ccttccttgg agctacctgg
    15181 gccattggag tgtacccccg ccagccagca gagtgtgtca aggaccagag actgcgcatg
    15241 ctcggttatc tgcccatcat ccgtttttcg acacagtgta ttaacgagct tttcatgaaa
    15301 ttcgctaaat tgcaacatag cacagcgttg tactctctcc ttagatatgg aagttttttt
    15361 gaatcgcctg attgtcactg aaacttcctg caagaggtcg cagcctcgaa gattttcaga
    15421 tttgcgagtg ccactggtgt tctcattctt aacattggaa gatttctcct tccccgcagc
    15481 cacttttgcg agcaaactag cgagggagta gcctttgttg ctctgatgtt tgagtgaagg
    15541 gaggcgaacg actggcctca tacccccgtt gcatatttcc tcagtccccc gttcgtttac
    15601 tgctttaacg tgtatgagga aagacctgta cttgcctccc gccatccctt tcaccagctt
    15661 tggactcgta agtgtaagca tccctgcgtg gccggacaag tccaggccgg aggccaacca
    15721 aacgggaccg cagagaatat cttctttgtg gtcttcaagg aagggacaaa gtctgaggca
    15781 ctgactttcc tcaacatcca tgtgttgcat ctcttcatta aggtcaacca atgagggttt
    15841 tccgttttgc tcgacttcta tattcaacgc attgactttt cccaaccccg gtgccttgtt
    15901 tttcagcaga gtaacttgaa tttgcggaat attagtaatg ttactattga ggacaaattt
    15961 gaagtcgaca tgtccaacca tgcatgcctt agggaggacc aattcgaata cgtgttcgtc
    16021 ccaactgttt gaatcggtct gcagtttcca cgttctcgta tgttgcgcgg catccccgtg
    16081 atgttgttgg tggaggtgtt gtgggtgccg tcgctgctgc tgctcttgct ggacttctac
    16141 ccaacaggga ggaacagttg cggagaatct tggagtcagg gtctcaaatc gagtcagctc
    16201 caccagtgaa gtcaagattt cgagcgtgaa cggttgatct accaacaggt caacgccaga
    16261 gatacccgga ctggaagggt tgctaagggg cttggagccc tcagaagacg gttctatccc
    16321 cttactaagg ctcccatcga ccaatatgtc ggcctcgtct atgtcgtcac atgtgcctcc
    16381 tatctgcaga aaatgaagtt ccccgccctt ggtgcacgcg caaaggcggt cagtgccact
    16441 acagtagatc actgagacga atgtgtcttg ctcttctgtt ccttcttttt tgggcggttc
    16501 aaccttcgcc aggatttcga aattgctcaa atcaaggatc ttaacgtagc cgccttgagt
    16561 cgtaattacc aggtgtccca gtgtactgat gtctgaagtc ttctcccgtt cgaagccgtt
    16621 tttggacgta agttgcatgt cctctattgg ttcctcacag tcgtcttccc gattgtcgag
    16681 gatgtcgggg gggaggagaa tgagtgatgt gatcgtgtct tgcgggtctt tgatatgttg
    16741 aatctttatg ggctcttcct caagagttac tatgcgtgta gcgtaattca ttttgtagag
    16801 caccaaatat cccccactta cgttcctttg ttcaggctga attatgaggg gagtttgaac
    16861 atcagcgggg gattcatgtt ggataacgga gatatttgca ccgttgacaa ccgccaaatt
    16921 ggattcgagt tcgcctttcc gtctattaca cagtgcgcta ttcagcttgt tcaggttgtt
    16981 caaagcttca acttggttta ttgctgagag gctctcgact gggcacgtgc gcagtcccac
    17041 cagcaaatga ataccatctg cacaaggcgt tattgagtct atgcaaagat tctcttcttc
    17101 cgcgaattta ggcagtcgca aacactgaac cagcgtacca ggcttgggga atactgaatt
    17161 ccatttctcg gaatcagggc tcgtgagatt tgctgcggcg tctgcgaatt gttgaatata
    17221 tgtaacaggt gggtcttgaa ggagcaattg ttcttgtgaa tccagttcca tctcgataat
    17281 ttgcgggacc gtgaagccat catcatgaag acttttggac ataatattgt tcatttggct
    17341 aaatattttc cctgcttttt catcgctctc cttgatagaa tacagaagca atacaggcaa
    17401 agtgcgtctg accaagggag aattgagctc gctattagta caggattcat tatccgttga
    17461 accctgctca ctaatggatt caccctgcgt tcgagaaagg ccatcgagtg acctatgaga
    17521 atttgaacta ataggtgaag tagctgggga tttgtatgtc agaagtccgc ccgcaagcag
    17581 acaggggaaa ggaatgttat gctgctcctg gtgcttctcc ttcgtttttt cagatttgga
    17641 gttagtcagg cagggattcg ctcccagttc ctcaagatct tttacagtgt cttcaagcac
    17701 attggccact atttcccatt ggagtttttc aggctgttgg aggatagaaa gcgcggtaat
    17761 gtcgagggag acttccatgg cctctttttg tgaggtatgc ccagtaacgc tatcggacct
    17821 gctgtgttct tcggagtctg aatcctcgag caggtcatca ctatctcctt cgagtttcgg
    17881 gatgtcagag caagatgatg aatcctcgag ccacgcaagc gtaggtcttc ggctatctac
    17941 accggaggag ggatcgccac tcaagataag ttgctgtacg atggcagggt catatgcatt
    18001 gatttcgaat ttcaaatgga ctttcataag ctttgagaca tcccagatgc atatttttcc
    18061 gcgtttggta gccgctgcaa ggaagtgtgg acaggacccg ctcccaaagc atgatattcg
    18121 atctgttccg tcggtacacg gaaattgtgc gggggacgta gccagagtaa cagagagtgg
    18181 tacattctgc gtgtgttctc ccttgacaaa cgggcaattc ggtgaatgcc gttcatgctc
    18241 gctccaaggc tcatcggtcg gctcccaaca taccaggcat actgagcagg taaagcacat
    18301 cgctctgtca tcacctgaag acgcaggttg atggtaaaat ccagcctggg ccatcgggtc
    18361 tggctgagcc catcgatagc cgacatgggg ccaggaggta aaagtttctc tccgattagc
    18421 ttcagagtac ataagtgatc gatcgacgga ccgcccgggg ccaactccca actctgggcg
    18481 cgcgctaggc aagaggtagc tcaagcgatc cataactgaa ctagccactg gaagggcagc
    18541 gacgttctga tttatttttt tcagctcgtt gactatagca gaagctatag atttaagcac
    18601 gtggtgagga aggtggaatg ttacggtcgc ccatttagct accttgtgat tcgctgctgt
    18661 ctcgtggctg gtattcttca ggccatcctt aagctgcgta atgaagaggt cgtaaccttc
    18721 ggtgctgctg atatctactt tctcgaggca ggcagagagc aattgctgtg cttcggtcac
    18781 ggggagttcc agctggacaa cgtcgtcctg cttactgacc ggggtctgga gcgccgtatc
    18841 cagcaggagt atcccattca aatctttccg acaccccacg gcatagtcat cgacgaaaat
    18901 aaccttatct actgctgaaa tgtactggca tttaacctgt ccgcctggtt tggctgacag
    18961 cgcggaggct tggagcgttg ccccacttgt tccatcaatg accttgatcg tgccgcggga
    19021 ggtaaccgcc agaatagcat taagcgctgg gtggtaggag agactatgca gcccgtctgc
    19081 atcgcaatgc atgcagccat ctcgcagtac cagccattcg ctaacccccg cagccccagc
    19141 tcctgcggca gaggaacaac caggtccgga ggcggcggca gcagctgcgg ccatctttcg
    19201 accggcagaa agcactatca cggatggcag cggctcagtg accgtgccgg gtggtgccgc
    19261 acccccaccc gttaccatgg ttcgcgaggg cccctggaac agaacttcca gcccaccgct
    19321 accaccgccg gatttttcga actgcgggtg gctccagtca cccatggtgg cggcactagg
    19381 cctggccaac taggcctggc caactttgga cctgggagtg gacacctgtg gagagaaagg
    19441 caaagtggat gtcattgtca ctcaagtgta tggccagatc gggccaggtg aatatcaaat
    19501 cctcctcgtt tttggaaact gacaatctta gcgcagaagt aatgcccgct tttg
//
