LOCUS       sequence-390544-        9187 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9187)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 9187)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2058
FEATURES             Location/Qualifiers
     source          1..9187
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    2125..2713
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2178..2198)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2596..2607)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2785..3018
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3096..3217
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3133..3152)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3187..3206
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3257..3392
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3412..3431)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3413..3431)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3441..3458)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3441..3457)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3450..3472)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3599..4054
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3686..3705)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3896..3917
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4080..4184
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4185..4253,4254..5045)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4403..4422)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5216..5804
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5705..5724
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5958..5975
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6092..6113
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6128..6133,6134..6151,6152..6158)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6166..6182
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6171..6193
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6190..6206
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6190..6206
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6225..6245
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6227..6245
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6273..6499
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6500..6680
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6727..6852
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7345..7578
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7763..7807
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8038..8248
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8060..8256
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8107..8242
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8288..8890
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8288..8307)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8784..8804
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8933..9050
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatgtc
     1201 gctgaccaac acaaagacgg ggttttcggt caaggacatc ttagacctgc cggacaccaa
     1261 cgatgaggag ggctctgtgg ccgaaggtcc ggaggaagag aacgaggggc ccgagccagc
     1321 caagagggcc gggccgctgg ggcagggcgc cctggacgcg gtgcagagcc tgcccctgaa
     1381 gaaccccttc tacgacagca gcgacaaccc gtacacgcgc tggctggcca gcaccgaggg
     1441 ccttcagtac tccctgcacg gtctggctgc cggggcgccc cctcaggact caagctccaa
     1501 gtccccggag ccctcggccg acgagtcacc ggacaatgac aaggagaccc cgggcggcgg
     1561 gggggacgcc ggcaagaagc gaaagcggcg agtgcttttc tccaaggcgc agacctacga
     1621 gctggagcgg cgctttcggc agcagcggta cctgtcggcg cccgagcgcg aacacctggc
     1681 cagcctcatc cgcctcacgc ccacgcaggt caagatctgg ttccagaacc accgctacaa
     1741 gatgaagcgc gcccgggccg agaaaggtat ggaggtgacg cccctgccct cgccgcgccg
     1801 ggtggccgtg cccgtcttgg tcagggacgg caaaccatgt cacgcgctca aagcccagga
     1861 cctggcagcc gccaccttcc aggcgggcat tcccttttct gcctacagcg cgcagtcgct
     1921 gcagcacatg cagtacaacg cccagtacag ctcggccagc accccccagt acccgacagc
     1981 acaccccctg gtccaggccc agcagtggac ttggtagtaa tgaactagtc cgtaacttga
     2041 aagtatttcg atttcttggc tttatatatc ttgtggaaag gacgaaacac gctggatccg
     2101 ccatgatgga cgcgttaagt cgacaatcaa cctctggatt acaaaatttg tgaaagattg
     2161 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     2221 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     2281 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     2341 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     2401 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     2461 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
     2521 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     2581 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     2641 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     2701 gccgcctccc cgcgtcgact ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     2761 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc
     2821 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     2881 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     2941 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     3001 tgtggaaaat ctctagcagt acgtatagta gttcatgtca tcttattatt cagtatttat
     3061 aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg
     3121 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     3181 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc
     3241 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     3301 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     3361 ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattc gccctatagt
     3421 gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
     3481 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
     3541 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac
     3601 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     3661 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     3721 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     3781 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     3841 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     3901 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     3961 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     4021 gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc
     4081 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     4141 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     4201 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     4261 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     4321 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     4381 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     4441 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     4501 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     4561 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     4621 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     4681 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     4741 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     4801 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     4861 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     4921 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     4981 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     5041 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     5101 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     5161 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     5221 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     5281 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     5341 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     5401 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     5461 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     5521 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     5581 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     5641 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     5701 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     5761 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     5821 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     5881 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     5941 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     6001 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     6061 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
     6121 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
     6181 aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact
     6241 aaagggaaca aaagctggag ctgcaagctt aatgtagtct tatgcaatac tcttgtagtc
     6301 ttgcaacatg gtaacgatga gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc
     6361 atgccgattg gtggaagtaa ggtggtacga tcgtgcctta ttaggaaggc aacagacggg
     6421 tctgacatgg attggacgaa ccactgaatt gccgcattgc agagatattg tatttaagtg
     6481 cctagctcga tacataaacg ggtctctctg gttagaccag atctgagcct gggagctctc
     6541 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     6601 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     6661 agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca
     6721 gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg
     6781 cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg
     6841 tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta
     6901 aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta
     6961 gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg
     7021 ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca
     7081 gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta
     7141 gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccgctgat
     7201 cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa
     7261 agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca
     7321 gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg
     7381 aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg
     7441 tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca
     7501 actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct
     7561 aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc
     7621 tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac
     7681 ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga
     7741 agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc
     7801 aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat
     7861 gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag
     7921 agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc
     7981 catgcattgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca
     8041 ggcagaagta tgcaaagcat gcgtctcaat tagtcagcaa ccatagtccc gcccctaact
     8101 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     8161 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     8221 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tttctagagg taccaccgga
     8281 gcttaccatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag
     8341 ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga
     8401 tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg
     8461 gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gccgtggcgg tctggaccac
     8521 gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt
     8581 gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc
     8641 caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg
     8701 tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc
     8761 cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt
     8821 caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg
     8881 tgccagttaa cgaattctag atcttgagac aaatggcagt attcatccac aattttaaaa
     8941 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
     9001 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt
     9061 acagggacag cagagatcca ctttggcgcc ggctcgaggc ctgcaggtgc aaagatggat
     9121 aaagttttaa acagagagga atctttgcag ctaatggacc ttctaggtct tgaaaggagt
     9181 gggaatt
//
