LOCUS       sequence-390548-        9658 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing the PBX2 transcription factor 
            ORF with a unique 24-bp barcode. Barcodes facilitate identification 
            of transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9658)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 9658)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0422
FEATURES             Location/Qualifiers
     source          1..9658
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1190..1199
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    2596..3184
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2649..2669)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3067..3078)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3256..3489
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3567..3688
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3604..3623)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3658..3677
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3728..3863
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3883..3902)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3884..3902)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3912..3929)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3912..3928)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3921..3943)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4070..4525
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4157..4176)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4367..4388
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4551..4655
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4656..4724,4725..5516)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4874..4893)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5687..6275
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6176..6195
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6429..6446
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6563..6584
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6599..6604,6605..6622,6623..6629)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6637..6653
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6642..6664
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6661..6677
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6661..6677
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6696..6716
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6698..6716
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6744..6970
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6971..7151
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7198..7323
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7816..8049
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8234..8278
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8509..8719
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8531..8727
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8578..8713
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8759..9361
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8759..8778)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9255..9275
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9404..9521
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatgga
     1201 cgaacggcta ctggggccgc cccctccagg cgggggccgg gggggcctgg gattggtgag
     1261 tggggagcct gggggccctg gcgagcctcc cggtggcgga gaccccggtg ggggtagcgg
     1321 gggggtcccg ggaggccgag ggaagcaaga catcggggac attctgcagc agataatgac
     1381 catcaccgac cagagcctgg acgaggccca ggccaagaaa cacgccctaa actgccaccg
     1441 aatgaagcct gctctcttta gcgtcctgtg tgaaatcaag gagaaaactg gcctcagcat
     1501 tcggagctcc caggaggagg agccggtgga cccacagctg atgcgcttgg acaacatgct
     1561 tctggcagag ggtgtggctg ggcccgagaa agggggcggc tcagcagcag cagctgcagc
     1621 cgctgcagcc tctggtggtg gtgtgtcccc tgacaactcc atcgaacact cggactatcg
     1681 cagcaaactt gcccagatcc gtcacatata ccactcggag ctggagaagt atgagcaggc
     1741 atgtaatgag ttcacgaccc atgtcatgaa cctgctgagg gagcagagcc gcaccaggcc
     1801 cgtggccccc aaagagatgg aacgcatggt gagcatcatc catcgaaagt tcagcgccat
     1861 ccagatgcag ctgaagcaga gcacctgcga ggctgtgatg atcctgcgct cccgtttcct
     1921 ggatgccaga cgaaagcgcc gtaacttcag caaacaggcc actgaggtcc taaatgagta
     1981 tttctactcc cacctgagta acccatatcc tagtgaggag gccaaggagg agcttgccaa
     2041 gaagtgtggc atcaccgtgt ctcaggtctc caactggttt ggcaacaaga ggattcgcta
     2101 taagaaaaac atcggaaagt tccaagagga ggcaaacatc tatgctgtca agaccgccgt
     2161 gtcagtcacc caggggggcc acagccgcac cagctccccg acaccccctt cctctgcagg
     2221 ctctggcggc tctttcaatc tctcaggatc tggagacatg tttctgggga tgcctgggct
     2281 caacggagat tcctattctg cttcccaggt ggaatcactc cgacactcga tggggccagg
     2341 gggctatggg gataacctcg ggggaggcca gatgtacagc ccacgggaaa tgagggcaaa
     2401 tggcagctgg caagaggctg tgaccccctc ttcagtgaca tccccaacgg agggaccagg
     2461 gagtgttcac tctgatacct ccaactagta atgaactagt ccgtaacttg aaagtatttc
     2521 gatttcttgg ctttatatat cttgtggaaa ggacgatgat gctgatcgtg ataggtggat
     2581 acgcgttaag tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     2641 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     2701 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     2761 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     2821 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     2881 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     2941 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc
     3001 tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     3061 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     3121 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     3181 ccgcgtcgac tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     3241 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg
     3301 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     3361 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     3421 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     3481 tctctagcag tacgtatagt agttcatgtc atcttattat tcagtattta taacttgcaa
     3541 agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat
     3601 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     3661 gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac
     3721 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     3781 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     3841 gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat
     3901 tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     3961 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     4021 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt
     4081 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     4141 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     4201 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     4261 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     4321 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     4381 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     4441 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     4501 aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc
     4561 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     4621 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     4681 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     4741 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     4801 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     4861 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     4921 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     4981 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     5041 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     5101 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     5161 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     5221 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     5281 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     5341 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     5401 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     5461 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     5521 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     5581 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     5641 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     5701 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     5761 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     5821 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     5881 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     5941 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     6001 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     6061 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     6121 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     6181 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     6241 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     6301 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     6361 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     6421 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     6481 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     6541 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     6601 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     6661 caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac
     6721 aaaagctgga gctgcaagct taatgtagtc ttatgcaata ctcttgtagt cttgcaacat
     6781 ggtaacgatg agttagcaac atgccttaca aggagagaaa aagcaccgtg catgccgatt
     6841 ggtggaagta aggtggtacg atcgtgcctt attaggaagg caacagacgg gtctgacatg
     6901 gattggacga accactgaat tgccgcattg cagagatatt gtatttaagt gcctagctcg
     6961 atacataaac gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     7021 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     7081 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     7141 aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc
     7201 tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg
     7261 gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc
     7321 gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg
     7381 ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc
     7441 gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta
     7501 caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc
     7561 ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata
     7621 gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc
     7681 tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa
     7741 aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa
     7801 aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat
     7861 gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca
     7921 gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt
     7981 ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca
     8041 acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg
     8101 gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga
     8161 gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca
     8221 aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg
     8281 gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg
     8341 aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca
     8401 gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccatgcattg
     8461 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     8521 atgcaaagca tgcgtctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     8581 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     8641 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     8701 ttttttggag gcctaggctt ttgcaaaaag ctttctagag gtaccaccgg agcttaccat
     8761 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg
     8821 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg
     8881 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
     8941 cggcaaggtg tgggtcgcgg acgacggcgc cgccgtggcg gtctggacca cgccggagag
     9001 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc
     9061 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
     9121 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag
     9181 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga
     9241 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
     9301 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgccagtta
     9361 acgaattcta gatcttgaga caaatggcag tattcatcca caattttaaa agaaaagggg
     9421 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
     9481 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca
     9541 gcagagatcc actttggcgc cggctcgagg cctgcaggtg caaagatgga taaagtttta
     9601 aacagagagg aatctttgca gctaatggac cttctaggtc ttgaaaggag tgggaatt
//
