LOCUS       sequence-390560-        9172 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 1 PhiC31 cassette with LR dvp promoter: 
            pGATA23-PhiC31-NLS-tUBQ1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9172)
  AUTHORS   Guiziou S, Maranas CJ, Chu JC, Nemhauser JL
  TITLE     An integrase toolbox to record gene-expression during plant 
            development.
  JOURNAL   Nat Commun. 2023 Apr 3;14(1):1844. doi: 10.1038/s41467-023-37607-5.
  PUBMED    37012288
REFERENCE   2  (bases 1 to 9172)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-04-3"; volume: "14(1):1844. doi"; pages: " 
            10.1038/s41467-023-37607-5"
COMMENT     Sequence Label: L1_GATA23_2
FEATURES             Location/Qualifiers
     source          1..9172
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    103..127
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(218..806)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(298..317)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2315..3130)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(2315..3130)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     3038..3057
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     3122..3141
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      3421..3856
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
     misc_feature    3983..4005
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
     polyA_signal    4013..4189
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(4421..5446)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(5466..5811)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(5503..5523)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     primer_bind     6014..6036
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6028..6045
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6029..6045
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6052..6071
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6052..6070
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             6885..8699
                     /codon_start=1
                     /product="integrase from phage phi-C31"
                     /label=phage phi-C31 integrase
                     /translation="MDTYAGAYDRQSRERENSSAASPATQRSANEDKAADLQREVERDG
                     GRFRFVGHFSEAPGTSAFGTAERPEFERILNECRAGRLNMIIVYDVSRFSRLKVMDAIP
                     IVSELLALGVTIVSTQEGVFRQGNVMDLIHLIMRLDASHKESSLKSAKILDTKNLQREL
                     GGYVGGKAPYGFELVSETKEITRNGRMVNVVINKLAHSTTPLTGPFEFEPDVIRWWWRE
                     IKTHKHLPFKPGSQAAIHPGSITGLCKRMDADAVPTRGETIGKKTASSAWDPATVMRIL
                     RDPRIAGFAAEVIYKKKPDGTPTTKIEGYRIQRDPITLRPVELDCGPIIEPAEWYELQA
                     WLDGRGRGKGLSRGQAILSAMDKLYCECGAVMTSKRGEESIKDSYRCRRRKVVDPSAPG
                     QHEGTCNVSMAALDKFVAERIFNKIRHAEGDEETLALLWEAARRFGKLTEAPEKSGERA
                     NLVAERADALNALEELYEDRAAGAYDGPVGRKHFRKQQAALTLRQQGAEERLAELEAAE
                     APKLPLDQWFPEDADADPTGPKSWWGRASVDDKRVFVGLFVDKIVVTKSTTGRGQGTPI
                     EKRASITWAKPPTDDDEDDAQDGTEDVAA"
     CDS             8700..8720
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 cattgaggct ctctacggtc acaggttgtg agccgttgtt aaagacaaac aaatgcgcta
       61 ccacagaatc aggggataac gcaggaaaga acatgaaggc cttgacagga tatattggcg
      121 ggtaaactaa gtcgctgtat gtgtttgttt gagatctcat gtgagcaaaa ggccagcaaa
      181 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
      241 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
      301 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
      361 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
      421 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
      481 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
      541 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
      601 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
      661 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaagaaga gttggtagct
      721 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
      781 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
      841 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
      901 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt actgagagat
      961 cccctcataa tttccccaaa gcgtaaccat gtgtgaataa attttgagct agtagggttg
     1021 cagccacgag taagtcttcc cttgttattg tgtagccaga atgccgcaaa acttccatgc
     1081 ctaagcgaac tgttgagagt acgtttcgat ttctgactgt gttagcctgg aagtgcttgt
     1141 cccaaccttg tttctgagca tgaacgcccg caagccaaca tgttagttga agcatcaggg
     1201 cgattagcag catgatatca aaacgctctg agctgctcgt tcggctatgg cgtaggccta
     1261 gtccgtaggc aggacttttc aagtctcgga aggtttcttc aatctgcatt cgcttcgaat
     1321 agatattaac aagttgtttg ggtgttcgaa tttcaacagg taagttagtt gctagaaccc
     1381 atggctcctt tgccgacgct gagtagattt taggtgacgg gtggtgacaa tgagtccgtg
     1441 tcgagcgctg attttttcgg cctttagagc gagatttata caatagaatt tggcatgaga
     1501 ttggattgct tttagtcagc ctcttatagc ctaaagtctt tgagtgacta gatgacatat
     1561 catgtaagtt gctgataggt ttccagtttt ccgctcctag gtctgcatat tgtacttttc
     1621 ctcttactcg acttaaccag taccaaccca gcttctcaac ggatttatac catggcactt
     1681 taaagccagc atcactgaca atgagcggtg tggtgttact cggtagaatg ctcgcaaggt
     1741 cggctagaaa ttggtcatga gctttctttg aacattgctc tgaaagcggg aacgctttct
     1801 cataaagagt aacagaacga ccgtgtagtg cgactgaagc tcgcaatacc ataagtcgtt
     1861 tttgctcacg aatatcagac cagtcaacaa gtacaatggg catcgtattg cccgaacaga
     1921 taaagctagc atgccaacgg tatacagcga gtcgctcttt gtggaggtga cgattaccta
     1981 acaatcggtc gattcgtttg atgttatgtt ttgttctcgc tttggttggc aggttacggc
     2041 caagttcggt aagagtgaga gttttacagt caagtaatgc gtggcaagcc aacgttaagc
     2101 tgttgagtcg ttttaagtgt aattcggggc agaattggta aagagagtcg tgtaaaatat
     2161 cgagttcgca catcttgttg tctgattatt gatttttcgc gaaaccattt gatcatatga
     2221 caagatgtgt atccacctta acttaatgat ttttaccaaa atcattaggg gattcatcag
     2281 tctaaagtat atatgtgtaa cattggtcta gtgattagaa aaactcatcg agcatcaaat
     2341 gaaactgcaa tttattcata tcaggattat caataccata tttttgaaaa agccgtttct
     2401 gtaatgaagg agaaaactca ccgaggcagt tccataggat ggcaagatcc tggtatcggt
     2461 ctgcgattcc gactcgtcca acatcaatac aacctattaa tttcccctcg tcaaaaataa
     2521 ggttatcaag tgagaaatca ccatgagtga cgactgaatc cggtgagaat ggcaaaagtt
     2581 tatgcatttc tttccagact tgttcaacag gccagccatt acgctcgtca tcaaaatcac
     2641 tcgcatcaac caaaccgtta ttcattcgtg attgcgcctg agcgagacga aatacgcgat
     2701 cgctgttaaa aggacaatta caaacaggaa tcgaatgcaa ccggcgcagg aacactgcca
     2761 gcgcatcaac aatattttca cctgaatcag gatattcttc taatacctgg aatgctgttt
     2821 tccctgggat cgcagtggtg agtaaccatg catcatcagg agtacggata aaatgcttga
     2881 tggtcggaag aggcataaat tccgtcagcc agtttagtct gaccatctca tctgtaacat
     2941 cattggcaac gctacctttg ccatgtttca gaaacaactc tggcgcatcg ggcttcccat
     3001 acaatcggta gattgtcgca cctgattgcc cgacattatc gcgagcccat ttatacccat
     3061 ataaatcagc atccatgttg gaatttaatc gcggccttga gcaagacgtt tcccgttgaa
     3121 tatggctcat aacacccctt gtattactgt ttatgtaagc agacagtttt attgttcatg
     3181 atgatatatt tttatcttgt gcaatgtaac atcagagatt ttgagacaca acgtggcttt
     3241 gttgaataaa tcgaactttt gctgagttga aggatcagat cacgcatctt cccgacaacg
     3301 cagaccgttc cgtggcaaag caaaagttca aaatcaccaa ctggtccacc tacaacaaag
     3361 ctctcatcaa ccgtggctcc ctcactttct ggctggatga tggggcgatt caggcgatcc
     3421 ccatccaaca gcccgccgtc gagcgggctt ttttatcccc ggaagcctgt ggatagaggg
     3481 tagttatcca cgtgaaaccg ctaatgcccc gcaaagcctt gattcacggg gctttccggc
     3541 ccgctccaaa aactatccac gtgaaatcgc taatcagggt acgtgaaatc gctaatcgga
     3601 gtacgtgaaa tcgctaataa ggtcacgtga aatcgctaat caaaaaggca cgtgagaacg
     3661 ctaatagccc tttcagatca acagcttgca aacacccctc gctccggcaa gtagttacag
     3721 caagtagtat gttcaattag cttttcaatt atgaatatat atatcaatta ttggtcgccc
     3781 ttggcttgtg gacaatgcgc tacgcgcacc ggctccgccc gtggacaacc gcaagcggtt
     3841 gcccaccgtc gagcgccttt gcccacaacc cggcggccgg ccgcaacaga tcgttttata
     3901 aatttttttt tttgaaaaag aaaaagcccg aaaggcggca acctctcggg cttctggatt
     3961 tccgatcccc ggaattagat cttggcagga tatattgtgg tgtaacgtta tcgatctgga
     4021 ttttagtact ggattttggt tttaggaatt agaaatttta ttgatagaag tattttacaa
     4081 atacaaatac atactaaggg tttcttatat gctcaacaca tgagcgaaac cctataagaa
     4141 ccctaatttc ccttatcggg aaactactca cacattattt atggagaaaa tagagagaga
     4201 tagatttgta gagagagact ggtgatttca gcgtaccgaa ttaattcctg aatcactgcg
     4261 accggccctc ccgcgaccca gccgagcgag cttagcgaac tgtggacgag aactgtgcca
     4321 ccaagcgtaa ggccgttctc tcgcatttgc cttgctaggc tcgcgcgagt tgctggctga
     4381 ggcgttctcg aaatcagctc ttgttcggtc ggcatctact ctattccttt gccctcggac
     4441 gagtgctggg gcgtcggttt ccactatcgg cgagtacttc tacacagcca tcggtccaga
     4501 cggccgcgct tctgcgggcg atttgtgtac gcccgacagt cccggctccg gatcggacga
     4561 ttgcgtcgca tcgaccctgc gcccaagctg catcatcgaa attgccgtca accaagctct
     4621 gatagagttg gtcaagacca atgcggagca tatacgcccg gagccttggc gatcctgcaa
     4681 gctccggatg cctccgctcg aagtagcgcg tctgctgctc catacaagcc aaccacggcc
     4741 tccagaagaa gatgttggcg acctcgtatt gggaatcccc gaacatcgcc tcgctccagt
     4801 caatgaccgc tgttatgcgg ccattgtccg tcaggacatt gttggagccg aaatccgcgt
     4861 gcacgaggtg ccggacttcg gggcagtcct cggcccaaag catcagctca tcgagagcct
     4921 gcgcgacgga cgcactgacg gtgtcgtcca tcacagtttg ccagtgatac acatggggat
     4981 cagcaatcgc gcatatgaaa tcacgccatg tagtgtattg accgattcct tgcggtccga
     5041 atgggccgaa cccgctcgtc tggctaagat cggccgcagc gatagcatcc atagcctccg
     5101 cgaccggctg aagaacagcg ggcagttcgg tttcaggcag gtcttgcaac gtgacaccct
     5161 gtgcacggcg ggagatgcaa taggtcaggc tctcgctgaa ctccccaatg tcaagcactt
     5221 ccggaatcgg gagcgcggcc gatgcaaagt gccgataaac ataacgatct ttgtagaaac
     5281 catcggcgca gctatttacc cgcaggacat atccacgccc tcctacatcg aagctgaaag
     5341 cacgagattc ttcgccctcc gagagctgca tcaggtcgga gacgctgtcg aacttttcga
     5401 tcagaaactt ctcgacagac gtcgcggtga gttcaggctt tttcataggg gggatcagct
     5461 tgggctgtcc tctccaaatg aaatgaactt ccttatatag aggaagggtc ttgcgaagga
     5521 tagtgggatt gtgcgtcatc ccttacgtca gtggagatgt cacatcaatc cacttgcttt
     5581 gaagacgtgg ttggaacgtc ttctttttcc acgatgctcc tcgtgggtgg gggtccatct
     5641 ttgggaccac tgtcggcaga ggcatcttga atgatagcct ttcctttatc gcaatgatgg
     5701 catttgtagg agccaccttc cttttctact gtcctttcga tgaagtgaca gatagctggg
     5761 caatggaatc cgaggaggtt tcccgaaatt accctttgtt gaaaagtctc aatagccctt
     5821 tggtcttctg agactgtatc tttgacattt ttggagtagg ggtacgataa cattaacgct
     5881 tacaatttcc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc
     5941 tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta
     6001 acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gtaatacgac
     6061 tcactatagg gcttggagca tgaataatct ttgcgtacac ctaaatttta tagtgaaaaa
     6121 tacataattt taaaacttta cttttgtaga gatgcctaaa aaaaaatggt ttaccacatg
     6181 gacattgtta tttgttcaga tctccaacgt atagcaatat ttgtgattta tagatatgta
     6241 atttggtgtt caaagaatgt gtagaacaat tattcttatg ggtatgccaa gaaaagttat
     6301 ataactttat tctatactgc tttcacgtta aataatgtaa cattattaat gttgtccttt
     6361 tacttttcct tgaattgatt tgggaagaag actattggct tccttatcac atcaaaaagt
     6421 ctatgagtaa gtgaaacatt ggctggcttt tgcttcattc ctttgtctct gtcttcatgt
     6481 gaacaagttt gcagaaattt gtggtttctt tttttacttt cttttgtcta atacacacac
     6541 tctcaccact attataaaga aaatccatag atcccggtcg tgcgtactgc cactttgctt
     6601 tccacccgac aatgatttgg gagatgtttt gttttgtgtc atacatctac caaaaaagtc
     6661 ttaatcattg ttcatcaaaa gagcgcccaa gggctctttc gtcttcttac cacacgcaca
     6721 ctagttcgag agggttttta aggagctctt aagcctttct tgatgactca taccacgaac
     6781 aagaaccaat cttgtcaccc atataatctc cgtattcaga gaattgaaga gttcttgatc
     6841 actactgttg tatcaaaaag gaactctaag aactaagatt gaaaatggac acgtatgccg
     6901 gcgcttatga tagacaatca agagaaagag agaactcttc tgccgcctct ccagctacac
     6961 agagatctgc caacgaggat aaagctgctg atttacaaag agaggtagag agagatgggg
     7021 gtaggtttag atttgtgggt catttctctg aggctcctgg tacatcagcc tttggtactg
     7081 ctgaacggcc tgaatttgaa agaatattaa atgagtgtcg ggccggtcga ctaaatatga
     7141 taatagttta tgatgtatct aggttcagta gactcaaagt tatggatgca attccgatcg
     7201 tgagcgagct acttgcactc ggtgttacta tcgtaagtac acaagaaggt gtattcaggc
     7261 agggtaatgt gatggacctt atacacctta ttatgcgact cgacgctagc cataaagagt
     7321 catcactgaa gagtgctaaa atcttggaca ccaagaacct gcaaagggag cttggaggct
     7381 atgtaggagg aaaagctccg tatggtttcg aactcgtgag tgagacaaaa gagattacca
     7441 gaaatggtag gatggtgaac gtggttatta ataagctagc tcatagcaca acccctctca
     7501 cagggccttt tgagttcgaa cctgacgtta ttagatggtg gtggagagag atcaaaactc
     7561 acaaacatct tccatttaag ccaggtagcc aggctgctat ccaccctgga tctatcactg
     7621 ggctttgtaa aaggatggat gctgacgcag tgccaactcg aggggaaaca atcggtaaga
     7681 agactgcatc ttccgcttgg gatcccgcta cagttatgag aatccttcgg gatccgcgta
     7741 ttgctggatt cgccgcagaa gtcatttata agaaaaaacc tgatggtact cctactacaa
     7801 aaatcgaagg ttataggatc caaagggatc ctattacttt gcgtccagtt gaattagact
     7861 gtggaccgat catcgaacct gccgagtggt acgagttgca ggcctggctt gatggtagag
     7921 ggcgtggaaa gggtttatct cggggacagg caattttgtc agcaatggat aagctttatt
     7981 gtgagtgtgg ggctgttatg acatcaaaac gtggagaaga gtcaatcaag gattcttaca
     8041 ggtgcagacg tcgtaaagtg gtggaccctt ctgctccagg acagcatgag ggtacatgca
     8101 acgtttctat ggctgctctt gataaatttg tggccgaacg tattttcaac aagattagac
     8161 acgctgaagg ggatgaggaa actttagctc ttctttggga ggctgcccgg cgttttggta
     8221 agctaacaga ggcaccagaa aaaagtggcg aaagagctaa tttggtagcc gaaagagcag
     8281 acgccttgaa tgcattggaa gaactttacg aagatagggc tgccggagca tatgatggac
     8341 cagttggtcg gaagcatttc aggaagcaac aagctgcact aactttgaga cagcaaggcg
     8401 cagaggagcg actcgcagag cttgaggccg ccgaggcccc aaagcttcca ctcgatcaat
     8461 ggtttcctga ggatgccgac gctgatccta caggtccaaa gagctggtgg ggaagagcta
     8521 gcgtagacga taagagagtg tttgtgggtc tatttgttga caagattgta gttactaagt
     8581 ccactacggg tcgaggacag ggcactccca tcgaaaagag agccagtatt acctgggcaa
     8641 agcctccaac cgatgatgat gaagacgatg cacaagatgg taccgaggat gtggcagcac
     8701 caaaaaaaaa gaggaaggtg taagcttaga gactcttatc aagaatccca tctcttgctt
     8761 gctttttttt gttgtcttcc ctttgatagg gtttgttttt cttgtttcag tgactttcta
     8821 tgttaaaaga taatgtcagt aaaaggattt ggttttctat tattctgaat cgattacgga
     8881 agattcttgc ttaattccaa tctatacaag tatcgtgaaa taatgaccgt ttatgtgatt
     8941 aggagacgtg tttcattaat aaaatataag atcaatacat tgttagtagt gataaactat
     9001 gtacaaattg tattgattgt aaaagaaaca caataggttc cttttttcta caatatattg
     9061 tgacagactc tctgttttaa cgaatgaatt aaatttgtca acgaaaaaat taaagaatga
     9121 tgtgatcact ttgcaccttg gttcatcttc agaggcttct gcatgttcca tg
//
