LOCUS       sequence-390576-        9058 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination vector for enhancer-driven split-intein 
            Gal4[1-20]N-int expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9058)
  AUTHORS   Ewen-Campen B, Luan H, Xu J, Singh R, Joshi N, Thakkar T, Berger B, 
            White BH, Perrimon N
  TITLE     split-intein Gal4 provides intersectional genetic labeling that is 
            repressible by Gal80.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Jun 13;120(24):e2304730120. doi: 
            10.1073/pnas.2304730120. Epub 2023 Jun 5.
  PUBMED    37276389
REFERENCE   2  (bases 1 to 9058)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-06-13"; volume: "120(24):e2304730120. 
            doi"; pages: " 10.1073/pnas.2304730120. Epub 2023 Jun 5"
COMMENT     Sequence Label: pBP-split-intein-Gal4[1-20]-N-int
FEATURES             Location/Qualifiers
     source          1..9058
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     36..54
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     protein_bind    82..206
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(231..236,237..254,255..261)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             315..974
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(381..400)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1316..1621
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1500..1519
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(1662..1786)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2552..2568)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2552..2568)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             complement(join(3579..4193,4264..4395,4599..4914,4976..5630,
                     5705..5978,6380..6451))
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     primer_bind     6903..6921
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     protein_bind    7146..7215
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     rep_origin      complement(7302..7890)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7382..7401)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8061..8852,8853..8921))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8684..8703
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8922..9026)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
       61 gtcttcaaga attcgtttat cacaagtttg tacaaaaaag ctgaacgaga aacgtaaaat
      121 gatataaata tcaatatatt aaattagatt ttgcataaaa aacagactac ataatactgt
      181 aaaacacaac atatccagtc actatggcgg ccgcattagg caccccaggc tttacacttt
      241 atgcttccgg ctcgtataat gtgtggattt tgagttagga tccgtcgaga ttttcaggag
      301 ctaaggaagc taaaatggag aaaaaaatca ctggatatac caccgttgat atatcccaat
      361 ggcatcgtaa agaacatttt gaggcatttc agtcagttgc tcaatgtacc tataaccaga
      421 ccgttcagct ggatattacg gcctttttaa agaccgtaaa gaaaaataag cacaagtttt
      481 atccggcctt tattcacatt cttgcccgcc tgatgaatgc tcatccggaa ttccgtatgg
      541 caatgaaaga cggtgagctg gtgatatggg atagtgttca cccttgttac accgttttcc
      601 atgagcaaac tgaaacgttt tcatcgctct ggagtgaata ccacgacgat ttccggcagt
      661 ttctacacat atattcgcaa gatgtggcgt gttacggtga aaacctggcc tatttcccta
      721 aagggtttat tgagaatatg tttttcgtct cagccaatcc ctgggtgagt ttcaccagtt
      781 ttgatttaaa cgtggccaat atggacaact tcttcgcccc cgttttcacc atgggcaaat
      841 attatacgca aggcgacaag gtgctgatgc cgctggcgat tcaggttcat catgccgttt
      901 gtgatggctt ccatgtcggc agaatgctta atgaattaca acagtactgc gatgagtggc
      961 agggcggggc gtaaacgcgt ggatccggct tactaaaagc cagataacag tatgcgtatt
     1021 tgcgcgctga tttttgcggt ataagaatat atactgatat gtatacccga agtatgtcaa
     1081 aaagaggtat gctatgaagc agcgtattac agtgacagtt gacagcgaca gctatcagtt
     1141 gctcaaggca tatatgatgt caatatctcc ggtctggtaa gcacaaccat gcagaatgaa
     1201 gcccgtcgtc tgcgtgccga acgctggaaa gcggaaaatc aggaagggat ggctgaggtc
     1261 gcccggttta ttgaaatgaa cggctctttt gctgacgaga acaggggctg gtgaaatgca
     1321 gtttaaggtt tacacctata aaagagagag ccgttatcgt ctgtttgtgg atgtacagag
     1381 tgatattatt gacacgcccg ggcgacggat ggtgatcccc ctggccagtg cacgtctgct
     1441 gtcagataaa gtctcccgtg aactttaccc ggtggtgcat atcggggatg aaagctggcg
     1501 catgatgacc accgatatgg ccagtgtgcc ggtctccgtt atcggggaag aagtggctga
     1561 tctcagccac cgcgaaaatg acatcaaaaa cgccattaac ctgatgttct ggggaatata
     1621 aatgtcaggc tcccttatac acagccagtc tgcaggtcga ccatagtgac tggatatgtt
     1681 gtgttttaca gtattatgta gtctgttttt tatgcaaaat ctaatttaat atattgatat
     1741 ttatatcatt ttacgtttct cgttcagctt tcttgtacaa agtggtgata aacggccggc
     1801 cgagctcgcc cggggatcga gcgcagcggt ataaaagggc gcggggtggc tgagagcatc
     1861 agttgtgaat gaatgttcga gccgagcaga cgtgccgctg ccttcgttaa tatcctttga
     1921 ataagccaac tttgaatcac aagacgcata ccaaacggta ccaaaacatg aagctactgt
     1981 cttctatcga acaagcatgc gatatttgcc gacttaaaaa gctcaagtgc ctggatctga
     2041 agacccaggt gcagaccccc cagggcatga aggagatcag caacatccag gtgggcgatc
     2101 tggtgctgag caacaccggc tacaacgagg tgctgaacgt gttccccaag agcaagaaga
     2161 agagctacaa gatcaccctg gaggatggca aggagatcat ctgcagcgag gagcacctgt
     2221 tccccaccca gaccggcgag atgaacatca gcggcggcct gaaggagggc atgtgcctgt
     2281 acgtgaagga gtaaaagctt atcgataccg tcgactaaag ccaaatagaa aattattcag
     2341 ttcctggctt aagtttttaa aagtgatatt atttatttgg ttgtaaccaa ccaaaagaat
     2401 gtaaataact aatacataat tatgttagtt ttaagttagc aacaaattga ttttagctat
     2461 attagctact tggttaataa atagaatata tttatttaaa gataattgcg tttttattgt
     2521 cagggagtga gtttgcttaa aaactcgttt agatccacta gttctagagc ggccgctggc
     2581 cacgggtgcg catgatcgtg ctcctgtcgt tgaggacccg gctaggctgg cggggttgcc
     2641 ttactggtta gcagaatgaa tcaccgatac gcgagcgaac gtgaagcgac tgctgctgca
     2701 aaacgtctgc gacctgagca acaacatgaa tggtcttcgg tttccgtgtt tcgtaaagtc
     2761 tggaaacgcg gaagtcagcg ccctgcacca ttatgttccg gaggcgcgcc ctagttccag
     2821 tgaaatccaa gcattttcta aattaaatgt attcttatta ttatagttgt tatttttgat
     2881 atatataaac aacactatta tgcccaccat ttttttgaga tgcatctaca caaggaacaa
     2941 acactggatg tcactttcag ttcaaattgt aacgctaatc actccgaaca ggtcacaaaa
     3001 aattacctta aaaagtcata atattaaatt agaataaata tagctgtgag ggaaatatat
     3061 acaaatatat tggagcaaat aaattgtaca tacaaatatt tattactaat ttctattgag
     3121 acgaaatgaa ccactcggaa ccatttgagc gaaccgaatc gcgcggaact aacgacagtc
     3181 gctccaaggt cgtcgaacaa aaggtgaatg tgttgcggag agcgggtggg agacagcgaa
     3241 agagcaacta cgaaacgtgg tgtggtggag gtgaattatg aagagggcgc gcgatttgaa
     3301 aagtatgtat ataaaaaata tatcccggtg ttttatgtag cgataaacga gtttttgatg
     3361 taaggtatgc aggtgtgtaa gtcttttggt tagaagacaa atccaaagtc tacttgtggg
     3421 gatgttcgaa ggggaaatac ttgtattcta taggtcatat cttgttttta ttggcacaaa
     3481 tataattaca ttagcttttt gagggggcaa taaacagtaa acacgatggt aataatggta
     3541 aaaaaaaaaa acaagcagtt atttcggata tatgtcggct actccttgcg tcgggcccga
     3601 agtcttagag ccagatatgc gagcacccgg aagctcacga tgagaatggc cagacccacg
     3661 tagtccagcg gcagatcggc ggcggagaag ttaagcgtct ccaggatgac cttgcccgaa
     3721 ctggggcacg tggtgttcga cgatgtgcag ctaatttcgc ccggctccac gtccgcccat
     3781 tggttaatca gcagaccctc gttggcgtaa cggaaccatg agaggtacga caaccatttg
     3841 aggtatactg gcaccgagcc cgagttcaag aagaagccgc caaagagcag gaatggtatg
     3901 ataaccggcg gacccacaga cagcgccatc gaggtcgagg agctggcgca ggatattaga
     3961 tatccgaagg acgttgacac attggccacc agagtgacca gcgccaggca gttgaagaag
     4021 tgcagcactc cggcccgcag tccgatcatc ggataggcaa tcgccgtgaa gaccagtggc
     4081 actgtgagaa aaagcggcaa ttcggcaatc gttttgccca gaaagtatgt gtcacagcga
     4141 taaagtcgac ttcgggcctc cctcataaaa actggcagct ctgaggtgaa cacctaaatc
     4201 gaatcgattc attagaaagt tagtaaatta ttgaaatgca aatgtattct aaacatgact
     4261 tacatttatc gtggcaaaga cgttttgaaa ggtcatgttg gtcaggaaga ggaagatggc
     4321 tccgttgata ttcatcacac ccacttgcgt gagttgttgg cccaaaaaga tgaggccaat
     4381 caagatggca accatctgca aattaaaatg ttactcgcat ctcattaata ttcgcgagtt
     4441 aaatgaaatt tatttatctt ctgcaaaact ataaactata catctcattg aaaaaaacta
     4501 agaagggtgt ggaatcaggc aattctatct aaaatctagc gaatttgttt ccaagaattg
     4561 taagcgttat atcatttgtt tccactggaa ccactcaccg ttgtctgaat aagtcgcact
     4621 tttacgagga gtggttcctt gagcaccgac agccaggatc gccacaggac cgcccggaac
     4681 tgcatgaacc aggtggcctt gtaggtgtac ccattctccg gctgctccag tggcttctcc
     4741 agatttttgg tggccaacaa ctgctccata tcccgggcta ctttgctaat ggcaaaattg
     4801 tcgcatatct tggcgatccg atcacgggac tcgatctccc gtccgggcac aacggccaac
     4861 acctgtacgt aaaagtccgc cggattgtag ttggtaggac actgggcacc cacgctggat
     4921 aggagttgag atgttatgta atactagata cccttaataa acacatcgaa ctcactagga
     4981 aaagaagtcg acggcttcgc tgggagtgcc caagaaagct accctgccct cggccatcag
     5041 aaggatcttg tcaaagagct caaacagctc ggaagacggc tgatgaatgg tcaggatgac
     5101 ggtcttgccc ttctgcgaca gcttcttcag cacctggacg acgctgtggg cggtaaagga
     5161 gtccagtccg gaggtgggct catcgcagat cagaagcggc ggatcggtta gagcctcgga
     5221 ggcgaatgcc agacgcttcc tttctccgcc ggacagacct ttcaccctgc cgggcacacc
     5281 gatgatcgtg tgctgacatt tgctgagcga aagctcctgg atcacctgat ccacgcgggc
     5341 cactcgctgc cgataggtca gatgtcgtgg catccgcacc atggcttgga aaatcaggtg
     5401 ttccctggcc gttagggagc cgataaagag gtcatcctgc tggacatagg cgcacctggc
     5461 ctgcatctcc ttggcgtcca caggttggcc attgagcagt cgcatcccgg atggcgatac
     5521 ttggatgccc tgcggcgatc gaaaggcaag ggcattcagc agggtcgtct ttccggcacc
     5581 ggaactgccc atcacggcca aaagttcgcc cggataggcc acgccgcaaa ctgagtttca
     5641 aattggtaat tggacccttt attaagattt cacacagatc agccgactgc gaatagaaac
     5701 tcaccgttct tgagcaaatg tttcctgggc gccggtatgt gtcgctcgtt gcagaatagt
     5761 ccgcgtgtcc ggttgaccag ctgccgccat ccggagcccg gctgattgac cgccccaaag
     5821 atgtccatat tgtgccaggc ataggtgagg ttctcggcta gttggccgct ccctgaaccg
     5881 gagtcctccg gcggactggg tggccggagc gtgccgtagt ttttggcctg cccgaagccc
     5941 tggttaatgc agctctgcga agccgctccg ctgtcaccct gcaatgatag gggatctcaa
     6001 atatcaacta caagcgttat gctcatctaa ccccgaacaa aaagtacccc gaagtatcct
     6061 acgaagtagg tttatacttt tatttatttt ttgtgcatct aggatcagct taaaatatct
     6121 ggttgttata ttttttgtaa aaaagaatat agtcgaaaat gaatgccttt agatgtcttg
     6181 atcatgatat gatctcaaaa attgtcttat atagcgagaa cagctaccag aataatctgt
     6241 ttcgtgtcac tatttgtttg tgcaattgcg gtttgggatt tttgtgggtc gcagttctca
     6301 cgccgcagac aatttgatgt tgcaatcgca gttcctatag atcaagtgaa cttaagatgt
     6361 atgcacatgt actactcaca ttgttcagat gctcggcaga tgggtgtttg ctgcctccgc
     6421 gaattaatag ctcctgatcc tcttggccca ttgccgggat ttttcacact ttcccctgct
     6481 tacccaccca aaaccaatca ccaccccaat cactcaaaaa acaaacaaaa ataagaagcg
     6541 agaggagttt tggcacagca ctttgtgttt aattgatggc gtaaaccgct tggagcttcg
     6601 tcacgaaacc gctgacaaaa tgcaactgaa ggcggacatt gacgctacgt aacgctacaa
     6661 acggtggcga aagagatagc ggacgcagcg gcgaaagaga cggcgatatt tctgtggaca
     6721 gagaaggagg caaacagcgc tgactttgag tggaatgtca ttttgagtga gaggtaatcg
     6781 aaagaacctg gtacatcaaa tacccttgga tcgaagtaaa tttaaaactg atcagataag
     6841 ttcaatgata tccagtgcag taaaaaaaaa aaatgttttt tttatctact ttccgcaaaa
     6901 atgggtttta ttaacttaca tacatactag gcgcgcccat atgttcggct tgtcgacatg
     6961 cccgccgtga ccgtcgagaa cccgctgacg ctgccccgcg tatccgcacc cgccgacgcc
     7021 gtcgcacgtc ccgtgctcac cgtgaccacc gcgcccagcg gtttcgaggg cgagggcttc
     7081 ccggtgcgcc gcgcgttcgc cgggatcaac taccgccacc tcgacccgtt catcatgatg
     7141 gaccagatgg gtgaggtgga gtacgcgccc ggggagccca agggcacgcc ctggcacccg
     7201 caccgcggct tcgagaccgt gacctacatc gtcgacggta acatgtgagc aaaaggccag
     7261 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     7321 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     7381 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     7441 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     7501 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     7561 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     7621 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     7681 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     7741 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     7801 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     7861 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     7921 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     7981 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     8041 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     8101 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     8161 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     8221 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     8281 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     8341 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     8401 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     8461 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     8521 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     8581 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     8641 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     8701 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     8761 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     8821 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     8881 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     8941 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     9001 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtct
//
