LOCUS       sequence-390582-       11428 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination vector for enhancer-driven split-intein 
            Gal4[21-881]C-int expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11428)
  AUTHORS   Ewen-Campen B, Luan H, Xu J, Singh R, Joshi N, Thakkar T, Berger B, 
            White BH, Perrimon N
  TITLE     split-intein Gal4 provides intersectional genetic labeling that is 
            repressible by Gal80.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Jun 13;120(24):e2304730120. doi: 
            10.1073/pnas.2304730120. Epub 2023 Jun 5.
  PUBMED    37276389
REFERENCE   2  (bases 1 to 11428)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-06-13"; volume: "120(24):e2304730120. 
            doi"; pages: " 10.1073/pnas.2304730120. Epub 2023 Jun 5"
COMMENT     Sequence Label: pBP-split-intein-Gal4[21-881]-C-int
FEATURES             Location/Qualifiers
     source          1..11428
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     36..54
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     protein_bind    82..206
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(231..236,237..254,255..261)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             315..974
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(381..400)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1316..1621
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1500..1519
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(1662..1786)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             4320..4664
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="activation domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 activation domain
                     /translation="ANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     primer_bind     complement(4922..4938)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4922..4938)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             complement(join(5949..6563,6634..6765,6969..7284,7346..8000,
                     8075..8348,8750..8821))
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     primer_bind     9273..9291
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     protein_bind    9516..9585
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     rep_origin      complement(9672..10260)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9752..9771)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10431..11222,11223..11291))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11054..11073
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11292..11396)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
       61 gtcttcaaga attcgtttat cacaagtttg tacaaaaaag ctgaacgaga aacgtaaaat
      121 gatataaata tcaatatatt aaattagatt ttgcataaaa aacagactac ataatactgt
      181 aaaacacaac atatccagtc actatggcgg ccgcattagg caccccaggc tttacacttt
      241 atgcttccgg ctcgtataat gtgtggattt tgagttagga tccgtcgaga ttttcaggag
      301 ctaaggaagc taaaatggag aaaaaaatca ctggatatac caccgttgat atatcccaat
      361 ggcatcgtaa agaacatttt gaggcatttc agtcagttgc tcaatgtacc tataaccaga
      421 ccgttcagct ggatattacg gcctttttaa agaccgtaaa gaaaaataag cacaagtttt
      481 atccggcctt tattcacatt cttgcccgcc tgatgaatgc tcatccggaa ttccgtatgg
      541 caatgaaaga cggtgagctg gtgatatggg atagtgttca cccttgttac accgttttcc
      601 atgagcaaac tgaaacgttt tcatcgctct ggagtgaata ccacgacgat ttccggcagt
      661 ttctacacat atattcgcaa gatgtggcgt gttacggtga aaacctggcc tatttcccta
      721 aagggtttat tgagaatatg tttttcgtct cagccaatcc ctgggtgagt ttcaccagtt
      781 ttgatttaaa cgtggccaat atggacaact tcttcgcccc cgttttcacc atgggcaaat
      841 attatacgca aggcgacaag gtgctgatgc cgctggcgat tcaggttcat catgccgttt
      901 gtgatggctt ccatgtcggc agaatgctta atgaattaca acagtactgc gatgagtggc
      961 agggcggggc gtaaacgcgt ggatccggct tactaaaagc cagataacag tatgcgtatt
     1021 tgcgcgctga tttttgcggt ataagaatat atactgatat gtatacccga agtatgtcaa
     1081 aaagaggtat gctatgaagc agcgtattac agtgacagtt gacagcgaca gctatcagtt
     1141 gctcaaggca tatatgatgt caatatctcc ggtctggtaa gcacaaccat gcagaatgaa
     1201 gcccgtcgtc tgcgtgccga acgctggaaa gcggaaaatc aggaagggat ggctgaggtc
     1261 gcccggttta ttgaaatgaa cggctctttt gctgacgaga acaggggctg gtgaaatgca
     1321 gtttaaggtt tacacctata aaagagagag ccgttatcgt ctgtttgtgg atgtacagag
     1381 tgatattatt gacacgcccg ggcgacggat ggtgatcccc ctggccagtg cacgtctgct
     1441 gtcagataaa gtctcccgtg aactttaccc ggtggtgcat atcggggatg aaagctggcg
     1501 catgatgacc accgatatgg ccagtgtgcc ggtctccgtt atcggggaag aagtggctga
     1561 tctcagccac cgcgaaaatg acatcaaaaa cgccattaac ctgatgttct ggggaatata
     1621 aatgtcaggc tcccttatac acagccagtc tgcaggtcga ccatagtgac tggatatgtt
     1681 gtgttttaca gtattatgta gtctgttttt tatgcaaaat ctaatttaat atattgatat
     1741 ttatatcatt ttacgtttct cgttcagctt tcttgtacaa agtggtgata aacggccggc
     1801 cgagctcgcc cggggatcga gcgcagcggt ataaaagggc gcggggtggc tgagagcatc
     1861 agttgtgaat gaatgttcga gccgagcaga cgtgccgctg ccttcgttaa tatcctttga
     1921 ataagccaac tttgaatcac aagacgcata ccaaacggta ccaaaacatg atgctgaaga
     1981 agatcctgaa gatcgaggag ctggatgagc gcgagctgat cgatatcgag gtgagcggca
     2041 accacctgtt ctacgccaac gatatcctga cccacaactg ctccaaagaa aaaccgaagt
     2101 gcgccaagtg tctgaagaac aactgggagt gtcgctactc tcccaaaacc aaaaggtctc
     2161 cgctgactag ggcacatctg acagaagtgg aatcaaggct agaaagactg gaacagctat
     2221 ttctactgat ttttcctcga gaagaccttg acatgatttt gaaaatggat tctttacagg
     2281 atataaaagc attgttaaca ggattatttg tacaagataa tgtgaataaa gatgccgtca
     2341 cagatagatt ggcttcagtg gagactgata tgcctctaac attgagacag catagaataa
     2401 gtgcgacatc atcatcggaa gagagtagta acaaaggtca aagacagttg actgtatcga
     2461 ttgactcggc agctcatcat gataactcca caattccgtt ggattttatg cccagggatg
     2521 ctcttcatgg atttgattgg tctgaagagg atgacatgtc ggatggcttg cccttcctga
     2581 aaacggaccc caacaataat gggttctttg gcgacggttc tctcttatgt attcttcgat
     2641 ctattggctt taaaccggaa aattacacga actctaacgt taacaggctc ccgaccatga
     2701 ttacggatag atacacgttg gcttctagat ccacaacatc ccgtttactt caaagttatc
     2761 tcaataattt tcacccctac tgccctatcg tgcactcacc gacgctaatg atgttgtata
     2821 ataaccagat tgaaatcgcg tcgaaggatc aatggcaaat cctttttaac tgcatattag
     2881 ccattggagc ctggtgtata gagggggaat ctactgatat agatgttttt tactatcaaa
     2941 atgctaaatc tcatttgacg agcaaggtct tcgagtcagg ttccataatt ttggtgacag
     3001 ccctacatct tctgtcgcga tatacacagt ggaggcagaa aacaaatact agctataatt
     3061 ttcacagctt ttccataaga atggccatat cattgggctt gaatagggac ctcccctcgt
     3121 ccttcagtga tagcagcatt ctggaacaaa gacgccgaat ttggtggtct gtctactctt
     3181 gggagatcca attgtccctg ctttatggtc gatccatcca gctttctcag aatacaatct
     3241 ccttcccttc ttctgtcgac gatgtgcagc gtaccacaac aggtcccacc atatatcatg
     3301 gcatcattga aacagcaagg ctcttacaag ttttcacaaa aatctatgaa ctagacaaaa
     3361 cagtaactgc agaaaaaagt cctatatgtg caaaaaaatg cttgatgatt tgtaatgaga
     3421 ttgaggaggt ttcgagacag gcaccaaagt ttttacaaat ggatatttcc accaccgctc
     3481 taaccaattt gttgaaggaa cacccttggc tatcctttac aagattcgaa ctgaagtgga
     3541 aacagttgtc tcttatcatt tatgtattaa gagatttttt cactaatttt acccagaaaa
     3601 agtcacaact agaacaggat caaaatgatc atcaaagtta tgaagttaaa cgatgctcca
     3661 tcatgttaag cgatgcagca caaagaactg ttatgtctgt aagtagctat atggacaatc
     3721 ataatgtcac cccatatttt gcctggaatt gttcttatta cttgttcaat gcagtcctag
     3781 tacccataaa gactctactc tcaaactcaa aatcgaatgc tgagaataac gagaccgcac
     3841 aattattaca acaaattaac actgttctga tgctattaaa aaaactggcc acttttaaaa
     3901 tccagacttg tgaaaaatac attcaagtac tggaagaggt atgtgcgccg tttctgttat
     3961 cacagtgtgc aatcccatta ccgcatatca gttataacaa tagtaatggt agcgccatta
     4021 aaaatattgt cggttctgca actatcgccc aataccctac tcttccggag gaaaatgtca
     4081 acaatatcag tgttaaatat gtttctcctg gctcagtagg gccttcacct gtgccattga
     4141 aatcaggagc aagtttcagt gatctagtca agctgttatc taaccgtcca ccctctcgta
     4201 actctccagt gacaatacca agaagcacac cttcgcatcg ctcagtcacg ccttttctag
     4261 ggcaacagca acagctgcaa tcattagtgc cactgacccc gtctgctttg tttggtggcg
     4321 ccaattttaa tcaaagtggg aatattgctg atagctcatt gtccttcact ttcactaaca
     4381 gtagcaacgg tccgaacctc ataacaactc aaacaaattc tcaagcgctt tcacaaccaa
     4441 ttgcctcctc taacgttcat gataacttca tgaataatga aatcacggct agtaaaattg
     4501 atgatggtaa taattcaaaa ccactgtcac ctggttggac ggaccaaact gcgtataacg
     4561 cgtttggaat cactacaggg atgtttaata ccactacaat ggatgatgta tataactatc
     4621 tattcgatga tgaagatacc ccaccaaacc caaaaaaaga gtaaaagctt atcgataccg
     4681 tcgactaaag ccaaatagaa aattattcag ttcctggctt aagtttttaa aagtgatatt
     4741 atttatttgg ttgtaaccaa ccaaaagaat gtaaataact aatacataat tatgttagtt
     4801 ttaagttagc aacaaattga ttttagctat attagctact tggttaataa atagaatata
     4861 tttatttaaa gataattgcg tttttattgt cagggagtga gtttgcttaa aaactcgttt
     4921 agatccacta gttctagagc ggccgctggc cacgggtgcg catgatcgtg ctcctgtcgt
     4981 tgaggacccg gctaggctgg cggggttgcc ttactggtta gcagaatgaa tcaccgatac
     5041 gcgagcgaac gtgaagcgac tgctgctgca aaacgtctgc gacctgagca acaacatgaa
     5101 tggtcttcgg tttccgtgtt tcgtaaagtc tggaaacgcg gaagtcagcg ccctgcacca
     5161 ttatgttccg gaggcgcgcc ctagttccag tgaaatccaa gcattttcta aattaaatgt
     5221 attcttatta ttatagttgt tatttttgat atatataaac aacactatta tgcccaccat
     5281 ttttttgaga tgcatctaca caaggaacaa acactggatg tcactttcag ttcaaattgt
     5341 aacgctaatc actccgaaca ggtcacaaaa aattacctta aaaagtcata atattaaatt
     5401 agaataaata tagctgtgag ggaaatatat acaaatatat tggagcaaat aaattgtaca
     5461 tacaaatatt tattactaat ttctattgag acgaaatgaa ccactcggaa ccatttgagc
     5521 gaaccgaatc gcgcggaact aacgacagtc gctccaaggt cgtcgaacaa aaggtgaatg
     5581 tgttgcggag agcgggtggg agacagcgaa agagcaacta cgaaacgtgg tgtggtggag
     5641 gtgaattatg aagagggcgc gcgatttgaa aagtatgtat ataaaaaata tatcccggtg
     5701 ttttatgtag cgataaacga gtttttgatg taaggtatgc aggtgtgtaa gtcttttggt
     5761 tagaagacaa atccaaagtc tacttgtggg gatgttcgaa ggggaaatac ttgtattcta
     5821 taggtcatat cttgttttta ttggcacaaa tataattaca ttagcttttt gagggggcaa
     5881 taaacagtaa acacgatggt aataatggta aaaaaaaaaa acaagcagtt atttcggata
     5941 tatgtcggct actccttgcg tcgggcccga agtcttagag ccagatatgc gagcacccgg
     6001 aagctcacga tgagaatggc cagacccacg tagtccagcg gcagatcggc ggcggagaag
     6061 ttaagcgtct ccaggatgac cttgcccgaa ctggggcacg tggtgttcga cgatgtgcag
     6121 ctaatttcgc ccggctccac gtccgcccat tggttaatca gcagaccctc gttggcgtaa
     6181 cggaaccatg agaggtacga caaccatttg aggtatactg gcaccgagcc cgagttcaag
     6241 aagaagccgc caaagagcag gaatggtatg ataaccggcg gacccacaga cagcgccatc
     6301 gaggtcgagg agctggcgca ggatattaga tatccgaagg acgttgacac attggccacc
     6361 agagtgacca gcgccaggca gttgaagaag tgcagcactc cggcccgcag tccgatcatc
     6421 ggataggcaa tcgccgtgaa gaccagtggc actgtgagaa aaagcggcaa ttcggcaatc
     6481 gttttgccca gaaagtatgt gtcacagcga taaagtcgac ttcgggcctc cctcataaaa
     6541 actggcagct ctgaggtgaa cacctaaatc gaatcgattc attagaaagt tagtaaatta
     6601 ttgaaatgca aatgtattct aaacatgact tacatttatc gtggcaaaga cgttttgaaa
     6661 ggtcatgttg gtcaggaaga ggaagatggc tccgttgata ttcatcacac ccacttgcgt
     6721 gagttgttgg cccaaaaaga tgaggccaat caagatggca accatctgca aattaaaatg
     6781 ttactcgcat ctcattaata ttcgcgagtt aaatgaaatt tatttatctt ctgcaaaact
     6841 ataaactata catctcattg aaaaaaacta agaagggtgt ggaatcaggc aattctatct
     6901 aaaatctagc gaatttgttt ccaagaattg taagcgttat atcatttgtt tccactggaa
     6961 ccactcaccg ttgtctgaat aagtcgcact tttacgagga gtggttcctt gagcaccgac
     7021 agccaggatc gccacaggac cgcccggaac tgcatgaacc aggtggcctt gtaggtgtac
     7081 ccattctccg gctgctccag tggcttctcc agatttttgg tggccaacaa ctgctccata
     7141 tcccgggcta ctttgctaat ggcaaaattg tcgcatatct tggcgatccg atcacgggac
     7201 tcgatctccc gtccgggcac aacggccaac acctgtacgt aaaagtccgc cggattgtag
     7261 ttggtaggac actgggcacc cacgctggat aggagttgag atgttatgta atactagata
     7321 cccttaataa acacatcgaa ctcactagga aaagaagtcg acggcttcgc tgggagtgcc
     7381 caagaaagct accctgccct cggccatcag aaggatcttg tcaaagagct caaacagctc
     7441 ggaagacggc tgatgaatgg tcaggatgac ggtcttgccc ttctgcgaca gcttcttcag
     7501 cacctggacg acgctgtggg cggtaaagga gtccagtccg gaggtgggct catcgcagat
     7561 cagaagcggc ggatcggtta gagcctcgga ggcgaatgcc agacgcttcc tttctccgcc
     7621 ggacagacct ttcaccctgc cgggcacacc gatgatcgtg tgctgacatt tgctgagcga
     7681 aagctcctgg atcacctgat ccacgcgggc cactcgctgc cgataggtca gatgtcgtgg
     7741 catccgcacc atggcttgga aaatcaggtg ttccctggcc gttagggagc cgataaagag
     7801 gtcatcctgc tggacatagg cgcacctggc ctgcatctcc ttggcgtcca caggttggcc
     7861 attgagcagt cgcatcccgg atggcgatac ttggatgccc tgcggcgatc gaaaggcaag
     7921 ggcattcagc agggtcgtct ttccggcacc ggaactgccc atcacggcca aaagttcgcc
     7981 cggataggcc acgccgcaaa ctgagtttca aattggtaat tggacccttt attaagattt
     8041 cacacagatc agccgactgc gaatagaaac tcaccgttct tgagcaaatg tttcctgggc
     8101 gccggtatgt gtcgctcgtt gcagaatagt ccgcgtgtcc ggttgaccag ctgccgccat
     8161 ccggagcccg gctgattgac cgccccaaag atgtccatat tgtgccaggc ataggtgagg
     8221 ttctcggcta gttggccgct ccctgaaccg gagtcctccg gcggactggg tggccggagc
     8281 gtgccgtagt ttttggcctg cccgaagccc tggttaatgc agctctgcga agccgctccg
     8341 ctgtcaccct gcaatgatag gggatctcaa atatcaacta caagcgttat gctcatctaa
     8401 ccccgaacaa aaagtacccc gaagtatcct acgaagtagg tttatacttt tatttatttt
     8461 ttgtgcatct aggatcagct taaaatatct ggttgttata ttttttgtaa aaaagaatat
     8521 agtcgaaaat gaatgccttt agatgtcttg atcatgatat gatctcaaaa attgtcttat
     8581 atagcgagaa cagctaccag aataatctgt ttcgtgtcac tatttgtttg tgcaattgcg
     8641 gtttgggatt tttgtgggtc gcagttctca cgccgcagac aatttgatgt tgcaatcgca
     8701 gttcctatag atcaagtgaa cttaagatgt atgcacatgt actactcaca ttgttcagat
     8761 gctcggcaga tgggtgtttg ctgcctccgc gaattaatag ctcctgatcc tcttggccca
     8821 ttgccgggat ttttcacact ttcccctgct tacccaccca aaaccaatca ccaccccaat
     8881 cactcaaaaa acaaacaaaa ataagaagcg agaggagttt tggcacagca ctttgtgttt
     8941 aattgatggc gtaaaccgct tggagcttcg tcacgaaacc gctgacaaaa tgcaactgaa
     9001 ggcggacatt gacgctacgt aacgctacaa acggtggcga aagagatagc ggacgcagcg
     9061 gcgaaagaga cggcgatatt tctgtggaca gagaaggagg caaacagcgc tgactttgag
     9121 tggaatgtca ttttgagtga gaggtaatcg aaagaacctg gtacatcaaa tacccttgga
     9181 tcgaagtaaa tttaaaactg atcagataag ttcaatgata tccagtgcag taaaaaaaaa
     9241 aaatgttttt tttatctact ttccgcaaaa atgggtttta ttaacttaca tacatactag
     9301 gcgcgcccat atgttcggct tgtcgacatg cccgccgtga ccgtcgagaa cccgctgacg
     9361 ctgccccgcg tatccgcacc cgccgacgcc gtcgcacgtc ccgtgctcac cgtgaccacc
     9421 gcgcccagcg gtttcgaggg cgagggcttc ccggtgcgcc gcgcgttcgc cgggatcaac
     9481 taccgccacc tcgacccgtt catcatgatg gaccagatgg gtgaggtgga gtacgcgccc
     9541 ggggagccca agggcacgcc ctggcacccg caccgcggct tcgagaccgt gacctacatc
     9601 gtcgacggta acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     9661 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     9721 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     9781 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     9841 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     9901 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     9961 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
    10021 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
    10081 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
    10141 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
    10201 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
    10261 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
    10321 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
    10381 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
    10441 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
    10501 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
    10561 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
    10621 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
    10681 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
    10741 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
    10801 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
    10861 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
    10921 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
    10981 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
    11041 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
    11101 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
    11161 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
    11221 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
    11281 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
    11341 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
    11401 tttccccgaa aagtgccacc tgacgtct
//
