LOCUS       sequence-390586-        7831 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insert to create split-intein Gal4[C-int] knock-in vectors via 
            Drop-In cloning, released via BsmBI digest.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7831)
  AUTHORS   Ewen-Campen B, Luan H, Xu J, Singh R, Joshi N, Thakkar T, Berger B, 
            White BH, Perrimon N
  TITLE     split-intein Gal4 provides intersectional genetic labeling that is 
            repressible by Gal80.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Jun 13;120(24):e2304730120. doi: 
            10.1073/pnas.2304730120. Epub 2023 Jun 5.
  PUBMED    37276389
REFERENCE   2  (bases 1 to 7831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-06-13"; volume: "120(24):e2304730120. 
            doi"; pages: " 10.1073/pnas.2304730120. Epub 2023 Jun 5"
COMMENT     Sequence Label: pDropIn-T2A-split-intein-Gal4[21-881]-C-int
FEATURES             Location/Qualifiers
     source          1..7831
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             378..722
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="activation domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 activation domain
                     /translation="ANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     protein_bind    974..1007
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      1233..1242
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1239..1916
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERAEGRHH
                     LFL"
     primer_bind     complement(1369..1387)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(1419..1439)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     1637..1656
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     1831..1854
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     polyA_signal    2037..2158
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2074..2093)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2128..2147
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    2188..2221
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(2310..2329)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2311..2329)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2336..2353)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2336..2352)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2345..2367)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             2490..2792
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     2671..2690
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             3141..3935
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3141..3935
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3195..3214)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3805..3824
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     regulatory      4135..4144
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4141..4515
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     rep_origin      4653..5241
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5142..5161
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5395..5412
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5529..5550
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5565..5570,5571..5588,5589..5595)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5603..5619
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5608..5630
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5627..5643
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5627..5643
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        5661..5679
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     5661..5678
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             5797..5850
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 gtgcgccgtt tctgttatca cagtgtgcaa tcccattacc gcatatcagt tataacaata
       61 gtaatggtag cgccattaaa aatattgtcg gttctgcaac tatcgcccaa taccctactc
      121 ttccggagga aaatgtcaac aatatcagtg ttaaatatgt ttctcctggc tcagtagggc
      181 cttcacctgt gccattgaaa tcaggagcaa gtttcagtga tctagtcaag ctgttatcta
      241 accgtccacc ctctcgtaac tctccagtga caataccaag aagcacacct tcgcatcgct
      301 cagtcacgcc ttttctaggg caacagcaac agctgcaatc attagtgcca ctgaccccgt
      361 ctgctttgtt tggtggcgcc aattttaatc aaagtgggaa tattgctgat agctcattgt
      421 ccttcacttt cactaacagt agcaacggtc cgaacctcat aacaactcaa acaaattctc
      481 aagcgctttc acaaccaatt gcctcctcta acgttcatga taacttcatg aataatgaaa
      541 tcacggctag taaaattgat gatggtaata attcaaaacc actgtcacct ggttggacgg
      601 accaaactgc gtataacgcg tttggaatca ctacagggat gtttaatacc actacaatgg
      661 atgatgtata taactatcta ttcgatgatg aagatacccc accaaaccca aaaaaagagt
      721 aacctctaga gtcgactaaa gccaaataga aattattcag ttctggctta agtttttaaa
      781 agtgatatta tttatttggt tgtaaccaac caaaagaatg taaataacta atacataatt
      841 atgttagttt taagttagca acaaattgat tttagctata ttagctactt ggttaataaa
      901 tagaatatat ttatttaaag ataattcgtt tttattgtca gggagtgagt ttgcttaaaa
      961 actcgtttgg atcataactt cgtataatgt atgctatacg aagttatcgt acgggatcta
     1021 attcaattag agactaattc aattagagct aattcaatta ggatccaagc ttatcgattt
     1081 cgaaccctcg accgccggag tataaataga ggcgcttcgt ctacggagcg acaattcaat
     1141 tcaaacaagc aaagtgaaca cgtcgctaag cgaaagctaa gcaaataaac aagcgcagct
     1201 gaacaagcta aacaatcggc tcgaagccgg tcgccaccat ggcctcctcc gaggacgtca
     1261 tcaaggagtt catgcgcttc aaggtgcgca tggagggctc cgtgaacggc cacgagttcg
     1321 agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg
     1381 tgaccaaggg cggccccctg cccttcgcct gggacatcct gtccccccag ttccagtacg
     1441 gctccaaggt gtacgtgaag caccccgccg acatccccga ctacaagaag ctgtccttcc
     1501 ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga
     1561 cccaggactc ctccctccag gacggctcct tcatctacaa ggtgaagttc atcggcgtga
     1621 acttcccctc cgacggcccc gtaatgcaga agaagactat gggctgggag gcgtccaccg
     1681 agcgcctgta cccccgcgac ggcgtgctga agggcgagat ccacaaggcc ctgaagctga
     1741 aggacggcgg ccactacctg gtggagttca agtccatcta catggccaag aagcccgtgc
     1801 agctgcccgg ctactactac gtggactcca agctggacat cacctcccac aacgaggact
     1861 acaccatcgt ggagcagtac gagcgcgccg agggccgcca ccacctgttc ctgtaggggc
     1921 cgcgactcta gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa
     1981 acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact
     2041 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     2101 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttaac
     2161 cgtctgtaag cagcgaaggc ccatacgata acttcgtata atgtatgcta tacgaagtta
     2221 tgagctccac gtctctcgcg aagggcgaat tctgcagata tccatcacac tggcggccgc
     2281 tcgagcatgc atctagaggg cccaattcgc cctatagtga gtcgtattac aattcactgg
     2341 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
     2401 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
     2461 cccaacagtt gcgcagccta tacgtacggc agtttaaggt ttacacctat aaaagagaga
     2521 gccgttatcg tctgtttgtg gatgtacaga gtgatattat tgacacgccg gggcgacgga
     2581 tggtgatccc cctggccagt gcacgtctgc tgtcagataa agtctcccgt gaactttacc
     2641 cggtggtgca tatcggggat gaaagctggc gcatgatgac caccgatatg gccagtgtgc
     2701 cggtctccgt tatcggggaa gaagtggctg atctcagcca ccgcgaaaat gacatcaaaa
     2761 acgccattaa cctgatgttc tggggaatat aaatgtcagg catgagatta tcaaaaagga
     2821 tcttcaccta gatccttttc acgtagaaag ccagtccgca gaaacggtgc tgaccccgga
     2881 tgaatgtcag ctactgggct atctggacaa gggaaaacgc aagcgcaaag agaaagcagg
     2941 tagcttgcag tgggcttaca tggcgatagc tagactgggc ggttttatgg acagcaagcg
     3001 aaccggaatt gccagctggg gcgccctctg gtaaggttgg gaagccctgc aaagtaaact
     3061 ggatggcttt cttgccgcca aggatctgat ggcgcagggg atcaagctct gatcaagaga
     3121 caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg
     3181 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg
     3241 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt
     3301 ccggtgccct gaatgaactg caagacgagg cagcgcggct atcgtggctg gccacgacgg
     3361 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat
     3421 tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat
     3481 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg
     3541 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg
     3601 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc
     3661 tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc
     3721 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg
     3781 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg
     3841 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca
     3901 tcgccttcta tcgccttctt gacgagttct tctgaattat taacgcttac aatttcctga
     3961 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatcagg tggcactttt
     4021 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     4081 ccgctcatga gacaataacc ctgataaatg cttcaataat agcacgtgag gagggccacc
     4141 atggccaagt tgaccagtgc cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc
     4201 gagttctgga ccgaccggct cgggttctcc cgggacttcg tggaggacga cttcgccggt
     4261 gtggtccggg acgacgtgac cctgttcatc agcgcggtcc aggaccaggt ggtgccggac
     4321 aacaccctgg cctgggtgtg ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag
     4381 gtcgtgtcca cgaacttccg ggacgcctcc gggccggcca tgaccgagat cggcgagcag
     4441 ccgtgggggc gggagttcgc cctgcgcgac ccggccggca actgcgtgca cttcgtggcc
     4501 gaggagcagg actgacacgt gctaaaactt catttttaat ttaaaaggat ctaggtgaag
     4561 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     4621 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     4681 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     4741 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     4801 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     4861 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     4921 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     4981 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     5041 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     5101 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     5161 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     5221 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     5281 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     5341 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     5401 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     5461 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
     5521 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
     5581 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat
     5641 gaccatgatt acgccaagct atttaggtga cactatagaa tactcaagct atgcatcaag
     5701 cttggtaccg agctcggatc cactagtaac ggccgccagt gtgctggaat tcgcccttaa
     5761 actgagacgg gtggaagcgg aggtagcgcg gccgccgagg gccgcggcag cctgctgacc
     5821 tgcggcgatg tggaggagaa ccccgggccc gctagcatga tgctgaagaa gatcctgaag
     5881 atcgaggagc tggatgagcg cgagctgatc gatatcgagg tgagcggcaa ccacctgttc
     5941 tacgccaacg atatcctgac ccacaactgc tccaaagaaa aaccgaagtg cgccaagtgt
     6001 ctgaagaaca actgggagtg tcgctactct cccaaaacca aaaggtctcc gctgactagg
     6061 gcacatctga cagaagtgga atcaaggcta gaaagactgg aacagctatt tctactgatt
     6121 tttcctcgag aagaccttga catgattttg aaaatggatt ctttacagga tataaaagca
     6181 ttgttaacag gattatttgt acaagataat gtgaataaag atgccgtcac agatagattg
     6241 gcttcagtgg agactgatat gcctctaaca ttgagacagc atagaataag tgcgacatca
     6301 tcatcggaag agagtagtaa caaaggtcaa agacagttga ctgtatcgat tgactcggca
     6361 gctcatcatg ataactccac aattccgttg gattttatgc ccagggatgc tcttcatgga
     6421 tttgattggt ctgaagagga tgacatgtcg gatggcttgc ccttcctgaa aacggacccc
     6481 aacaataatg ggttctttgg cgacggttct ctcttatgta ttcttcgatc tattggcttt
     6541 aaaccggaaa attacacgaa ctctaacgtt aacaggctcc cgaccatgat tacggataga
     6601 tacacgttgg cttctagatc cacaacatcc cgtttacttc aaagttatct caataatttt
     6661 cacccctact gccctatcgt gcactcaccg acgctaatga tgttgtataa taaccagatt
     6721 gaaatcgcgt cgaaggatca atggcaaatc ctttttaact gcatattagc cattggagcc
     6781 tggtgtatag agggggaatc tactgatata gatgtttttt actatcaaaa tgctaaatct
     6841 catttgacga gcaaggtctt cgagtcaggt tccataattt tggtgacagc cctacatctt
     6901 ctgtcgcgat atacacagtg gaggcagaaa acaaatacta gctataattt tcacagcttt
     6961 tccataagaa tggccatatc attgggcttg aatagggacc tcccctcgtc cttcagtgat
     7021 agcagcattc tggaacaaag acgccgaatt tggtggtctg tctactcttg ggagatccaa
     7081 ttgtccctgc tttatggtcg atccatccag ctttctcaga atacaatctc cttcccttct
     7141 tctgtcgacg atgtgcagcg taccacaaca ggtcccacca tatatcatgg catcattgaa
     7201 acagcaaggc tcttacaagt tttcacaaaa atctatgaac tagacaaaac agtaactgca
     7261 gaaaaaagtc ctatatgtgc aaaaaaatgc ttgatgattt gtaatgagat tgaggaggtt
     7321 tcgagacagg caccaaagtt tttacaaatg gatatttcca ccaccgctct aaccaatttg
     7381 ttgaaggaac acccttggct atcctttaca agattcgaac tgaagtggaa acagttgtct
     7441 cttatcattt atgtattaag agattttttc actaatttta cccagaaaaa gtcacaacta
     7501 gaacaggatc aaaatgatca tcaaagttat gaagttaaac gatgctccat catgttaagc
     7561 gatgcagcac aaagaactgt tatgtctgta agtagctata tggacaatca taatgtcacc
     7621 ccatattttg cctggaattg ttcttattac ttgttcaatg cagtcctagt acccataaag
     7681 actctactct caaactcaaa atcgaatgct gagaataacg agaccgcaca attattacaa
     7741 caaattaaca ctgttctgat gctattaaaa aaactggcca cttttaaaat ccagacttgt
     7801 gaaaaataca ttcaagtact ggaagaggta t
//
