LOCUS       sequence-39059-e         660 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 660)
  AUTHORS   Aoyama M, Agari K, Sun-Wada GH, Futai M, Wada Y
  TITLE     Simple and straightforward construction of a mouse gene targeting 
            vector using in vitro transposition reactions.
  JOURNAL   Nucleic Acids Res. 2005  . 33(5):e52.
  PUBMED    15784610
REFERENCE   2  (bases 1 to 660)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2005  . 33(5):e52."
COMMENT     Sequence Label: pGPS21loxFRTNeo
FEATURES             Location/Qualifiers
     source          1..660
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    252..299
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     protein_bind    complement(322..355)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 cagagctctt actgtcatgc catccgtatg tgggcggaca ataaagtctt aaactgaaca
       61 aaatagatct aaactatgac aataaagtct taaactagac agaatagttg taaactgaaa
      121 tcagtccagt tatgctgtga aaaagcatac tggacttttg ttatggctaa agcaaactct
      181 tcattttctg aagtgcaaat tgcccgtcgt attaaagagg ggcgtggggt cccgggctgc
      241 aggaattctt tgaagttcct attccgaagt tcctattctc tagaaagtat aggaacttca
      301 gaattcgatc ggccggatcc tataacttcg tatagcatac attatacgaa gttatctcga
      361 ccagcttctg atggaattag aacttggcaa aacaatactg agaatgaagt gtatgtggaa
      421 cagaggctgc tgatctcgtt cttcaggcta tgaaactgac acatttggaa accacagtac
      481 ttagaaccac aaagtgggaa tcaagagaaa aacaatgatc ccacgagaga tctatagatc
      541 tatagatcat gagtgggagg aatgagctgg cccttaattt ggttttgctt gtttaaatta
      601 tgatatccaa ctatgaaaca ttatcataaa gcaatagtaa agagccttca gtaaagagca
//
