LOCUS       sequence-390594-        2189 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Kanamycin resistant cloning vector backbone for pDSP23, pDSP31-33, 
            pHJL1-4, pSiM1-4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2189)
  TITLE     plasmids optimized for C. elegans expression
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pDSP1
FEATURES             Location/Qualifiers
     source          1..2189
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     143..159
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     143..159
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(400..416)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     418..440
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(478..496)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        564..666
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             667..1482
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCLKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(740..759)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      join(1653..2189,1..52)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2142..2161
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
       61 caacgcggcc tttttacggt tcctggcctt ttgctggcct ttttacggtt cctggccttt
      121 tgctggcctt ttgctcacat gtcaggaaac agctatgaca tcaagctgac tagataatct
      181 agctgatcgt ggaccgatca tacgtataat gccgtaagat cacgcgtcgc agcacagctc
      241 gcggtccagt agtgatatct gtcgcccgtc tcaaacgcaa ccctccggcg gccgcatatc
      301 attcaggacg agcctcagac tccagcgtaa ctggactgca atcaactcac tggctcacct
      361 tccggtccac gatcagctag aatcaagctg actagataaa ctggccgtcg ttttactccg
      421 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc
      481 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
      541 gtggcacttt tcggggaaat gtgtgatcgg cacgtaagag gttccaactt tcaccataat
      601 gaaataagat cactaccggg cgtatttttt gagttatcga gattttcagg agctaaggaa
      661 gctaaaatga gccatattca acgggaaacg tcttgcttga agccgcgatt aaattccaac
      721 atggatgctg atttatatgg gtataaatgg gctcgcgata atgtcgggca atcaggtgcg
      781 acaatctatc gattgtatgg gaagcccgat gcgccagagt tgtttctgaa acatggcaaa
      841 ggtagcgttg ccaatgatgt tacagatgag atggtcaggc taaactggct gacggaattt
      901 atgcctcttc cgaccatcaa gcattttatc cgtactcctg atgatgcatg gttactcacc
      961 actgcgatcc cagggaaaac agcattccag gtattagaag aatatcctga ttcaggtgaa
     1021 aatattgttg atgcgctggc agtgttcctg cgccggttgc attcgattcc tgtttgtaat
     1081 tgtcctttta acggcgatcg cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac
     1141 ggtttggttg gtgcgagtga ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc
     1201 tggaaagaaa tgcataaact cttgccattc tcaccggatt cagtcgtcac tcatggtgat
     1261 ttctcacttg ataaccttat ttttgacgag gggaaattaa taggttgtat tgatgttgga
     1321 cgagtcggaa tcgcagaccg ataccaggat cttgccatcc tatggaactg cctcggtgag
     1381 ttttctcctt cattacagaa acggcttttt caaaaatatg gtattgataa tcctgatatg
     1441 aataaattgc agtttcactt gatgctcgat gagtttttct aactgtcaga ccaagtttac
     1501 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     1561 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     1621 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     1681 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     1741 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     1801 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     1861 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     1921 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     1981 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     2041 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     2101 gacagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     2161 tatagtcctg tcgggtttcg ccacctctg
//
