LOCUS       sequence-390611-        8428 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fluorescent reporter for genetic tracing of epithelial cell 
            SARS-CoV2 response..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8428)
  AUTHORS   Jiang B, Schmitt MJ, Rand U, Company C, Dramaretska Y, Grossmann M, 
            Serresi M, Cicin-Sain L, Gargiulo G
  TITLE     Pharmacological modulators of epithelial immunity uncovered by 
            synthetic genetic tracing of SARS-CoV-2 infection responses.
  JOURNAL   Sci Adv. 2023 Jun 23;9(25):eadf4975. doi: 10.1126/sciadv.adf4975. 
            Epub 2023 Jun 21.
  PUBMED    37343108
REFERENCE   2  (bases 1 to 8428)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2023-06-23"; volume: "9(25):eadf4975. doi"; pages: " 
            10.1126/sciadv.adf4975. Epub 2023 Jun 21"
COMMENT     Sequence Label: pPB-Neo-COVGT5-d2EGFP-mCherry
FEATURES             Location/Qualifiers
     source          1..8428
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   5..39
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    316..336
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    1844..1868
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      1869..1878
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1875..1877,1878..1880,1881..2591,2592..2597,
                     2598..2720)
                     /codon_start=1
                     /product="EGFP destabilized by fusion to residues 422-461 
                     of mouse ornithine decarboxylase, giving an in vivo 
                     half-life of ~2 hours"
                     /label=d2EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYKKLSHGFPPEVEEQDDGTLPMSCAQESGMDRHPAACASARINV
                     "
     primer_bind     complement(1920..1941)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2181..2200)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2528..2549
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(2721..2745)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(2823..2842)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2888..2943
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2942..2961)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     enhancer        3298..3677
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3678..3881
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3832..3852
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             3914..4708
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3914..4708
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3968..3987)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4578..4597
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4746..4763)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4752..4976
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   5326..5388
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(5454..5473)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5455..5473)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5480..5497)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5480..5496)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5489..5511)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5638..6093
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5725..5744)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5935..5956
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6119..6223
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6224..6292,6293..7084)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6442..6461)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7255..7843
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7744..7763
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7997..8014
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8131..8152
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8167..8172,8173..8190,8191..8197)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8205..8221
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8210..8232
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8229..8245
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8229..8245
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        8266..8284
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaaccctag aaagatagtc tgcgtaaaat tgacgcatgc attcttgaaa tattgctctc
       61 tctttctaaa tagcgcgaat ccgtcgctgt gcatttagga catctcagtc gccgcttgga
      121 gctcccgtga ggcgtgcttg tcaatgcggt aagtgtcact gattttgaac tataacgacc
      181 gcgtgagtca aaatgacgca tgattatctt ttacgtgact tttaagattt aactcatacg
      241 ataattatat tgttatttca tgttctactt acgtgataac ttattatata tatattttct
      301 tgttatagat atcatcaact ttgtatagaa aagttgtgga agcagccggc aggagaattg
      361 cttgaaccca ggaggcggag gtagcggtga gctgagattg caccactgca ctccagcctg
      421 ggcaataaaa gtgaaactcc atctcaaaaa caaccacaca cacagagaag agaaaagaga
      481 aaagaataag aaagaatggg aagcaaggag atgaaggaga ggaagtcgta gctgggaagg
      541 ggaccaggaa gtgggtgtca ggtccaagag ctgctagaaa gaaacgaaac tgaaagcagg
      601 gaatttccca agtttgggga agacaggaac tgcagcgccc ctccccgttg gaacatttgc
      661 tctgaattaa gaagaaatgt tgttatttac atctgaccta ttttctctag acctcagtgg
      721 tttccaggac aacacactga ggttttgaag gaggggtgca aggggaagtg ctgactcatc
      781 ttttttgcca ctcccttccc accctgtccc cagaggtttt tctgtccctt tatgtctatg
      841 aacaagtaca gctgaggcca tcagcactgc acacacctgg aacagtcttg ttaaaacagg
      901 acctccctaa aggccaagag ctaaggaaag agaaagtgaa ggactgagca ggcagctgag
      961 ccagaggtca ggctgtagtg gggaggccag aaggggcggg gacagccggg attgggaggg
     1021 gagaacagca gcgtgggcgg ctggcagagg cagggaaaga ccagcagaga ggaaagagaa
     1081 actgggagag ggaggaaggg agggcacctg gcatggacat gcctgggcag accccatgcc
     1141 aaatttcatc ctgtctcata gctttccatt ccgtttcttt ttcagtttct cattgttact
     1201 tcctgccctg ctctggtttg gtgagttttg tccttccttt ggataaaaac aatgtcccca
     1261 cacccagtgt tgtgtaatca gggaatgaaa gaatctgctg tgatgccagc actcaagggg
     1321 acagtttctg ttgctgtggg aacctgaact ttgcatttcc tgactttggg gtaattaaaa
     1381 tgtcagcctt ccagatgctg acgcaacgag gtgtgggagg gagtcagggg gcgtgtccag
     1441 gaggttggcc aggccaatcg gaggcggggc cagagggttg actgggcagg ccgggggcgg
     1501 ggctggaata ataataagag atggtagggg cgggtgtagg ctttgcttta tggtgatgcc
     1561 aggacagttt tgttttccat ttccctgtgc cataaaacgg ctttcttgga aagctaccag
     1621 atattcccca ctaaaagtct tcaaggtcac tccgtggaaa agggagggct acttttgaag
     1681 ggggtcactt tccttcagct atgggggcag ctgagccaga ggtcaggctg tagtggggag
     1741 gccagaaggg gcggggacag ccgggattgg gaggggagaa cagcagcgtg ggcggctggc
     1801 agaggcaggg aaagaccagc agagaggaaa gagaaactgg gagacaagtt tgtacaaaaa
     1861 agcaggctgc caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
     1921 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
     1981 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
     2041 tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc
     2101 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
     2161 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
     2221 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
     2281 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg
     2341 acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca
     2401 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
     2461 tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc
     2521 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
     2581 agctgtacaa gaagcttagc catggcttcc cgccggaggt ggaggagcag gatgatggca
     2641 cgctgcccat gtcttgtgcc caggagagcg ggatggaccg tcaccctgca gcctgtgctt
     2701 ctgctaggat caatgtgtag acccagcttt cttgtacaaa gtggtgatcc tcaggtgcag
     2761 gctgcctatc agaaggtggt ggctggtgtg gccaatgccc tggctcacaa ataccactga
     2821 gatctttttc cctctgccaa aaattatggg gacatcatga agccccttga gcatctgact
     2881 tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt tttgtgtctc
     2941 tcactcggaa ggacatatgg gagggcaaat catttaaaac atcagaatga gtatttggtt
     3001 tagagtttgg caacatatgc ccatatgctg gctgccatga acaaaggttg gctataaaga
     3061 ggtcatcagt atatgaaaca gccccctgct gtccattcct tattccatag aaaagccttg
     3121 acttgaggtt agattttttt tatattttgt tttgtgttat ttttttcttt aacatcccta
     3181 aaattttcct tacatgtttt actagccaga tttttcctcc tctcctgact actcccagtc
     3241 atagctgtcc ctcttctctt atggagatcc ctcgacctgc agcccaagct tcgcgttgac
     3301 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
     3361 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
     3421 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
     3481 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
     3541 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
     3601 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
     3661 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
     3721 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
     3781 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
     3841 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tctctggcta actagagaac
     3901 ccactgcgcc accatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt
     3961 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt
     4021 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc
     4081 cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc
     4141 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga
     4201 agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat
     4261 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca
     4321 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga
     4381 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc
     4441 gagcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat
     4501 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga
     4561 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg
     4621 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt
     4681 ctatcgcctt cttgacgagt tcttctgact cgagtctaga gggcccgttt aaacccgctg
     4741 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc
     4801 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc
     4861 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa
     4921 gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggctcg
     4981 agttaattaa cgagagcata atattgatat gtgccaaagt tgtttctgac tgactaataa
     5041 gtataatttg tttctattat gtataggtta agctaattac ttattttata atacaacatg
     5101 actgttttta aagtacaaaa taagtttatt tttgtaaaag agagaatgtt taaaagtttt
     5161 gttactttat agaagaaatt ttgagttttt gttttttttt aataaataaa taaacataaa
     5221 taaattgttt gttgaattta ttattagtat gtaagtgtaa atataataaa acttaatatc
     5281 tattcaaatt aataaataaa cctcgatata cagaccgata aaacacatgc gtcaatttta
     5341 cgcatgatta tctttaacgt acgtcacaat atgattatct ttctagggtt aaataatagt
     5401 ttctaatttt tttattattc agcctgctgt cgtgaatacc gagctccaat tcgccctata
     5461 gtgagtcgta ttacaattca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
     5521 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
     5581 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg
     5641 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     5701 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     5761 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     5821 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     5881 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     5941 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     6001 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     6061 cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg
     6121 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     6181 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     6241 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     6301 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     6361 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     6421 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     6481 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     6541 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     6601 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     6661 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     6721 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     6781 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     6841 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     6901 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     6961 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     7021 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     7081 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     7141 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     7201 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     7261 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     7321 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     7381 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     7441 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     7501 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     7561 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     7621 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     7681 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     7741 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     7801 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     7861 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     7921 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     7981 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
     8041 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
     8101 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
     8161 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
     8221 atttcacaca ggaaacagct atgaccatga ttacgccaag ctcgaaatta accctcacta
     8281 aagggaacaa aagctggtac ctcgcgcgac ttggtttgcc attctttagc gcgcgtcgcg
     8341 tcacacagct tggccacaat gtggtttttg tcaaacgaag attctatgac gtgtttaaag
     8401 tttaggtcga gtaaagcgca aatctttt
//
