LOCUS       sequence-390618-        9231 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insert to create split-intein Gal4[21-881]-C-int knock-in vectors, 
            with G418 resistance, via Drop-In cloning, released via BsmBI 
            digest.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9231)
  AUTHORS   Ewen-Campen B, Luan H, Xu J, Singh R, Joshi N, Thakkar T, Berger B, 
            White BH, Perrimon N
  TITLE     split-intein Gal4 provides intersectional genetic labeling that is 
            repressible by Gal80.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Jun 13;120(24):e2304730120. doi: 
            10.1073/pnas.2304730120. Epub 2023 Jun 5.
  PUBMED    37276389
REFERENCE   2  (bases 1 to 9231)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-06-13"; volume: "120(24):e2304730120. 
            doi"; pages: " 10.1073/pnas.2304730120. Epub 2023 Jun 5"
COMMENT     Sequence Label: pDropIn-T2A-split-intein-Gal4[21-881]-C-int-G418R
FEATURES             Location/Qualifiers
     source          1..9231
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             384..728
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="activation domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 activation domain
                     /translation="ANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     protein_bind    980..1013
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      1239..1248
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1245..1922
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERAEGRHH
                     LFL"
     primer_bind     complement(1375..1393)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(1425..1445)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     1643..1662
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     1837..1860
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     polyA_signal    2043..2164
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2080..2099)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2134..2153
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        2181..2632
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     2580..2601
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     protein_bind    3594..3627
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(3716..3735)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3717..3735)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3742..3759)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3742..3758)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3751..3773)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             3896..4198
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     4077..4096
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             4547..5341
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4547..5341
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4601..4620)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5211..5230
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     regulatory      5541..5550
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5547..5921
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     rep_origin      6059..6647
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6548..6567
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6801..6818
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6935..6956
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6971..6976,6977..6994,6995..7001)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7009..7025
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7014..7036
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7033..7049
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7033..7049
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        7067..7085
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     7067..7084
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             7203..7256
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 aggtatgtgc gccgtttctg ttatcacagt gtgcaatccc attaccgcat atcagttata
       61 acaatagtaa tggtagcgcc attaaaaata ttgtcggttc tgcaactatc gcccaatacc
      121 ctactcttcc ggaggaaaat gtcaacaata tcagtgttaa atatgtttct cctggctcag
      181 tagggccttc acctgtgcca ttgaaatcag gagcaagttt cagtgatcta gtcaagctgt
      241 tatctaaccg tccaccctct cgtaactctc cagtgacaat accaagaagc acaccttcgc
      301 atcgctcagt cacgcctttt ctagggcaac agcaacagct gcaatcatta gtgccactga
      361 ccccgtctgc tttgtttggt ggcgccaatt ttaatcaaag tgggaatatt gctgatagct
      421 cattgtcctt cactttcact aacagtagca acggtccgaa cctcataaca actcaaacaa
      481 attctcaagc gctttcacaa ccaattgcct cctctaacgt tcatgataac ttcatgaata
      541 atgaaatcac ggctagtaaa attgatgatg gtaataattc aaaaccactg tcacctggtt
      601 ggacggacca aactgcgtat aacgcgtttg gaatcactac agggatgttt aataccacta
      661 caatggatga tgtatataac tatctattcg atgatgaaga taccccacca aacccaaaaa
      721 aagagtaacc tctagagtcg actaaagcca aatagaaatt attcagttct ggcttaagtt
      781 tttaaaagtg atattattta tttggttgta accaaccaaa agaatgtaaa taactaatac
      841 ataattatgt tagttttaag ttagcaacaa attgatttta gctatattag ctacttggtt
      901 aataaataga atatatttat ttaaagataa ttcgttttta ttgtcaggga gtgagtttgc
      961 ttaaaaactc gtttggatca taacttcgta taatgtatgc tatacgaagt tatcgtacgg
     1021 gatctaattc aattagagac taattcaatt agagctaatt caattaggat ccaagcttat
     1081 cgatttcgaa ccctcgaccg ccggagtata aatagaggcg cttcgtctac ggagcgacaa
     1141 ttcaattcaa acaagcaaag tgaacacgtc gctaagcgaa agctaagcaa ataaacaagc
     1201 gcagctgaac aagctaaaca atcggctcga agccggtcgc caccatggcc tcctccgagg
     1261 acgtcatcaa ggagttcatg cgcttcaagg tgcgcatgga gggctccgtg aacggccacg
     1321 agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc
     1381 tgaaggtgac caagggcggc cccctgccct tcgcctggga catcctgtcc ccccagttcc
     1441 agtacggctc caaggtgtac gtgaagcacc ccgccgacat ccccgactac aagaagctgt
     1501 ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga
     1561 ccgtgaccca ggactcctcc ctccaggacg gctccttcat ctacaaggtg aagttcatcg
     1621 gcgtgaactt cccctccgac ggccccgtaa tgcagaagaa gactatgggc tgggaggcgt
     1681 ccaccgagcg cctgtacccc cgcgacggcg tgctgaaggg cgagatccac aaggccctga
     1741 agctgaagga cggcggccac tacctggtgg agttcaagtc catctacatg gccaagaagc
     1801 ccgtgcagct gcccggctac tactacgtgg actccaagct ggacatcacc tcccacaacg
     1861 aggactacac catcgtggag cagtacgagc gcgccgaggg ccgccaccac ctgttcctgt
     1921 aggggccgcg actctagatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt
     1981 taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg
     2041 ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     2101 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     2161 cttaaccgtc tgtaagcagc ctagaatccc aaaacaaact ggttattgtg gtaggtcatt
     2221 tgtttggcag aaagaaaact cgagaaattt ctctggccgt tattcgttat tctctctttt
     2281 ctttttgggt cgctccctct ctgcactaat gctctctcac tctgtcacac agtaaacggc
     2341 atactgctct cgttggttcg agagagcgcg cctcgaatgt tcgcgaaaag agcgccggag
     2401 tataaataga ggcgcttcgt ctacggagcg acaattcaat tcaaacaagc aaagtgaaca
     2461 cgtcgctaag cgaaagctaa gcaaataaac aagcgcagct gaacaagcta aacaatctgc
     2521 agtaaagtgc aagttaaagt gaatcaatta aaagtaacca gcaaccaagt aaatcaactg
     2581 caactactga aatctgccaa gaagtaatta ttgaatacaa gaagagaact ctcaatactc
     2641 gaatccatgt gggagtttat tcttgacaca gatatttatg atataataac tgagtaagct
     2701 taacataagg aggaaaacaa aatgattgag caggatggac tgcacgctgg ctcgccagct
     2761 gcctgggtgg agcgtctgtt cggctacgat tgggcccagc agaccatcgg ctgctccgat
     2821 gctgccgtgt tccgtctgtc ggcccaggga cgccccgtgc tgttcgtcaa gaccgacctg
     2881 agtggagccc tgaacgagct ccaggatgag gcagctcgtc tgagctggct ggccacaaca
     2941 ggagtgccgt gtgctgccgt gctggatgtg gtgaccgaag ctggacgcga ttggctgctg
     3001 ctgggcgagg tgccaggcca ggacctgctg tccagccatc tggccccagc cgagaaggtg
     3061 tccattatgg ccgatgctat gcgtcgcctg cacaccctgg acccagccac ctgccccttt
     3121 gaccaccagg ccaagcaccg tattgagcgt gcccgaaccc gtatggaggc tggactggtg
     3181 gaccaggatg acctggatga ggagcaccag ggcctggctc cagccgagct gtttgcccgt
     3241 ctgaaggccc gtatgcccga tggcgaggac ctggtggtga cccacggcga cgcctgcctg
     3301 cccaacatta tggtggagaa cggacgcttc agtggcttca ttgattgtgg acgcctgggc
     3361 gtggccgacc gttaccagga tatcgccctg gccacccgag atatcgccga ggagctggga
     3421 ggcgagtggg ccgaccgatt cctggtgctc tacggcattg cagccccaga tagccagcgt
     3481 attgccttct accgtctgct ggacgagttc ttctaagctt ggcaataaaa agacagaata
     3541 aaacgcacgg gtgttgggtc gtttgttccg ctgtcaggag gaaggcccat acgataactt
     3601 cgtataatgt atgctatacg aagttatgag ctccacgtct ctcgcgaagg gcgaattctg
     3661 cagatatcca tcacactggc ggccgctcga gcatgcatct agagggccca attcgcccta
     3721 tagtgagtcg tattacaatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
     3781 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
     3841 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctatacg tacggcagtt
     3901 taaggtttac acctataaaa gagagagccg ttatcgtctg tttgtggatg tacagagtga
     3961 tattattgac acgccggggc gacggatggt gatccccctg gccagtgcac gtctgctgtc
     4021 agataaagtc tcccgtgaac tttacccggt ggtgcatatc ggggatgaaa gctggcgcat
     4081 gatgaccacc gatatggcca gtgtgccggt ctccgttatc ggggaagaag tggctgatct
     4141 cagccaccgc gaaaatgaca tcaaaaacgc cattaacctg atgttctggg gaatataaat
     4201 gtcaggcatg agattatcaa aaaggatctt cacctagatc cttttcacgt agaaagccag
     4261 tccgcagaaa cggtgctgac cccggatgaa tgtcagctac tgggctatct ggacaaggga
     4321 aaacgcaagc gcaaagagaa agcaggtagc ttgcagtggg cttacatggc gatagctaga
     4381 ctgggcggtt ttatggacag caagcgaacc ggaattgcca gctggggcgc cctctggtaa
     4441 ggttgggaag ccctgcaaag taaactggat ggctttcttg ccgccaagga tctgatggcg
     4501 caggggatca agctctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga
     4561 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc
     4621 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc
     4681 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcaag acgaggcagc
     4741 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac
     4801 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc
     4861 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac
     4921 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg
     4981 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct
     5041 cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt
     5101 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg
     5161 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac
     5221 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg
     5281 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg
     5341 aattattaac gcttacaatt tcctgatgcg gtattttctc cttacgcatc tgtgcggtat
     5401 ttcacaccgc atcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     5461 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     5521 aataatagca cgtgaggagg gccaccatgg ccaagttgac cagtgccgtt ccggtgctca
     5581 ccgcgcgcga cgtcgccgga gcggtcgagt tctggaccga ccggctcggg ttctcccggg
     5641 acttcgtgga ggacgacttc gccggtgtgg tccgggacga cgtgaccctg ttcatcagcg
     5701 cggtccagga ccaggtggtg ccggacaaca ccctggcctg ggtgtgggtg cgcggcctgg
     5761 acgagctgta cgccgagtgg tcggaggtcg tgtccacgaa cttccgggac gcctccgggc
     5821 cggccatgac cgagatcggc gagcagccgt gggggcggga gttcgccctg cgcgacccgg
     5881 ccggcaactg cgtgcacttc gtggccgagg agcaggactg acacgtgcta aaacttcatt
     5941 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     6001 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     6061 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     6121 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     6181 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     6241 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     6301 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     6361 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     6421 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     6481 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     6541 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     6601 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     6661 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     6721 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     6781 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     6841 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     6901 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
     6961 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
     7021 aacaatttca cacaggaaac agctatgacc atgattacgc caagctattt aggtgacact
     7081 atagaatact caagctatgc atcaagcttg gtaccgagct cggatccact agtaacggcc
     7141 gccagtgtgc tggaattcgc ccttaaactg agacgggtgg aagcggaggt agcgcggccg
     7201 ccgagggccg cggcagcctg ctgacctgcg gcgatgtgga ggagaacccc gggcccgcta
     7261 gcatgatgct gaagaagatc ctgaagatcg aggagctgga tgagcgcgag ctgatcgata
     7321 tcgaggtgag cggcaaccac ctgttctacg ccaacgatat cctgacccac aactgctcca
     7381 aagaaaaacc gaagtgcgcc aagtgtctga agaacaactg ggagtgtcgc tactctccca
     7441 aaaccaaaag gtctccgctg actagggcac atctgacaga agtggaatca aggctagaaa
     7501 gactggaaca gctatttcta ctgatttttc ctcgagaaga ccttgacatg attttgaaaa
     7561 tggattcttt acaggatata aaagcattgt taacaggatt atttgtacaa gataatgtga
     7621 ataaagatgc cgtcacagat agattggctt cagtggagac tgatatgcct ctaacattga
     7681 gacagcatag aataagtgcg acatcatcat cggaagagag tagtaacaaa ggtcaaagac
     7741 agttgactgt atcgattgac tcggcagctc atcatgataa ctccacaatt ccgttggatt
     7801 ttatgcccag ggatgctctt catggatttg attggtctga agaggatgac atgtcggatg
     7861 gcttgccctt cctgaaaacg gaccccaaca ataatgggtt ctttggcgac ggttctctct
     7921 tatgtattct tcgatctatt ggctttaaac cggaaaatta cacgaactct aacgttaaca
     7981 ggctcccgac catgattacg gatagataca cgttggcttc tagatccaca acatcccgtt
     8041 tacttcaaag ttatctcaat aattttcacc cctactgccc tatcgtgcac tcaccgacgc
     8101 taatgatgtt gtataataac cagattgaaa tcgcgtcgaa ggatcaatgg caaatccttt
     8161 ttaactgcat attagccatt ggagcctggt gtatagaggg ggaatctact gatatagatg
     8221 ttttttacta tcaaaatgct aaatctcatt tgacgagcaa ggtcttcgag tcaggttcca
     8281 taattttggt gacagcccta catcttctgt cgcgatatac acagtggagg cagaaaacaa
     8341 atactagcta taattttcac agcttttcca taagaatggc catatcattg ggcttgaata
     8401 gggacctccc ctcgtccttc agtgatagca gcattctgga acaaagacgc cgaatttggt
     8461 ggtctgtcta ctcttgggag atccaattgt ccctgcttta tggtcgatcc atccagcttt
     8521 ctcagaatac aatctccttc ccttcttctg tcgacgatgt gcagcgtacc acaacaggtc
     8581 ccaccatata tcatggcatc attgaaacag caaggctctt acaagttttc acaaaaatct
     8641 atgaactaga caaaacagta actgcagaaa aaagtcctat atgtgcaaaa aaatgcttga
     8701 tgatttgtaa tgagattgag gaggtttcga gacaggcacc aaagttttta caaatggata
     8761 tttccaccac cgctctaacc aatttgttga aggaacaccc ttggctatcc tttacaagat
     8821 tcgaactgaa gtggaaacag ttgtctctta tcatttatgt attaagagat tttttcacta
     8881 attttaccca gaaaaagtca caactagaac aggatcaaaa tgatcatcaa agttatgaag
     8941 ttaaacgatg ctccatcatg ttaagcgatg cagcacaaag aactgttatg tctgtaagta
     9001 gctatatgga caatcataat gtcaccccat attttgcctg gaattgttct tattacttgt
     9061 tcaatgcagt cctagtaccc ataaagactc tactctcaaa ctcaaaatcg aatgctgaga
     9121 ataacgagac cgcacaatta ttacaacaaa ttaacactgt tctgatgcta ttaaaaaaac
     9181 tggccacttt taaaatccag acttgtgaaa aatacattca agtactggaa g
//
