LOCUS       sequence-390630-        4938 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Honey bee gut symbiont broad-host range vector with constitutive 
            GFP, ampR and pBBR1 replicon.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4938)
  AUTHORS   Chhun A, Moriano-Gutierrez S, Zoppi F, Cabirol A, Engel P, Schaerli 
            Y
  TITLE     An engineered bacterial symbiont allows noninvasive biosensing of 
            the honey bee gut environment.
  JOURNAL   PLoS Biol. 2024 Mar 5;22(3):e3002523. doi: 
            10.1371/journal.pbio.3002523. eCollection 2024 Mar.
  PUBMED    38442124
REFERENCE   2  (bases 1 to 4938)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Biol."; date: "2024-03-5"; volume: "22(3):e3002523. doi"; pages: " 
            10.1371/journal.pbio.3002523. eCollection 2024 Mar"
COMMENT     Sequence Label: pAC12
FEATURES             Location/Qualifiers
     source          1..4938
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(47..712)
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRRT"
     rep_origin      713..1484
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     terminator      2735..2771
                     /label=soxR terminator
                     /note="bidirectional E. coli soxR transcription terminator"
     CDS             complement(join(3923..4714,4715..4783))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4546..4565
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4784..4888)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 acgcccggta gtgatcttat ttcattatgg tgaaagttgg aacctcttac gtacggcgcg
       61 gcagcgtgac ccgtgtcggc ggctccaacg gctcgccatc gtccagaaaa cacggctcat
      121 cgggcatcgg caggcgctgc tgcccgcgtc gttcccattc ctccgtttcg gtcaaggctg
      181 gcaggtctgg ttccatgccc ggaatgccgg gctggctggg cggctcctcg ccggggccgg
      241 tcggtagttg ctgctcgccc ggatacaggg tcgggatgcg gcgcaggtcg ccatgcccca
      301 acagcgattc gtcctggtcg tcgtgatcaa ccaccacggc ggcactgaac accgacaggc
      361 gcaactggtc gcggggctgg ccccacgcca cgcggtcatt gaccacgtag gccgacacgg
      421 tgccggggcc gttgagcttc acgacggaga tccagcgctc ggccaccaag tccttgactg
      481 cgtattggac cgtccgcaaa gaacgtccga tgagcttgga aagtgtcttc tggctgacca
      541 ccacggcgtt ctggtggccc atctgcgcca cgaggtgatg cagcagcatt gccgccgtgg
      601 gtttcctcgc aataagcccg gcccacgcct catgcgcttt gcgttccgtt tgcacccagt
      661 gaccgggctt gttcttggct tgaatgccga tttctctgga ctgcgtggcc atgcttatct
      721 ccatgcggta ggggtgccgc acggttgcgg caccatgcgc aatcagctgc aacttttcgg
      781 cagcgcgaca acaattatgc gttgcgtaaa agtggcagtc aattacagat tttctttaac
      841 ctacgcaatg agctattgcg gggggtgccg caatgagctg ttgcgtaccc ccctttttta
      901 agttgttgat ttttaagtct ttcgcatttc gccctatatc tagttctttg gtgcccaaag
      961 aagggcaccc ctgcggggtt cccccacgcc ttcggcgcgg ctccccctcc ggcaaaaagt
     1021 ggcccctccg gggcttgttg atcgactgcg cggccttcgg ccttgcccaa ggtggcgctg
     1081 cccccttgga acccccgcac tcgccgccgt gaggctcggg gggcaggcgg gcgggcttcg
     1141 cccttcgact gcccccactc gcataggctt gggtcgttcc aggcgcgtca aggccaagcc
     1201 gctgcgcggt cgctgcgcga gccttgaccc gccttccact tggtgtccaa ccggcaagcg
     1261 aagcgcgcag gccgcaggcc ggaggctttt ccccagagaa aattaaaaaa attgatgggg
     1321 caaggccgca ggccgcgcag ttggagccgg tgggtatgtg gtcgaaggct gggtagccgg
     1381 tgggcaatcc ctgtggtcaa gctcgtgggc aggcgcagcc tgtccatcag cttgtccagc
     1441 agggttgtcc acgggccgag cgaagcgagc cagccggtgg ccgctcgcgg ccatcgtcca
     1501 catatccacg ggctggcaag ggagcgcagc gaccgcgcag ggcgaagccc ggagagcaag
     1561 cccgtagggc gccgcagccg ccgtaggcgg tcacgacttt gcgaagcaaa gtctagtgag
     1621 tatactcaag cattgagtgg cccgccggag gcaccgcctt gcgctgcccc cgtcgagccg
     1681 gttggacacc aaaagggagg ggcaggcatg gcggcatacg cgatcatgcg atgcaagaag
     1741 ctggcgaaaa tgggcaacgt ggcggccagt ctcaagcacg cctaccgcga gcgcgagacg
     1801 cccaacgctg acgccagcag gacgccagag aacgagcact gggcggccag cagcaccgat
     1861 gaagcgatgg gccgactgcg cgagttgctg ccagagaagc ggcgcaagga cgctgtgttg
     1921 gcggtcgagt acgtcatgac ggccagcccg gaatggtgga agtcggccag ccaagaacag
     1981 caggcggcgt tcttcgagaa ggcgcacaag tggctggcgg acaagtacgg ggcggatcgc
     2041 atcgtgacgg ccagcatcca ccgtgacgaa accagcccgc acatgaccgc gttcgtggtg
     2101 ccgctgacgc aggacggcag gctgtcggcc aaggagttca tcggcaacaa agcgcagatg
     2161 acccgcgacc agaccacgtt tgcggccgct gtggccgatc tagggctgca acggggcatc
     2221 gagggcagca aggcacgtca cacgcgcatt caggcgttct acgaggccct ggagcggcca
     2281 ccagtgggcc acgtcaccat cagcccgcaa gcggtcgagc cacgcgccta tgcaccgcag
     2341 ggattggccg aaaagctggg aatctcaaag cgcgttgaga cgccggaagc cgtggccgac
     2401 cggctgacaa aagcggttcg gcaggggtat gagcctgccc tacaggccgc cgcaggagcg
     2461 cgtgagatgc gcaagaaggc cgatcaagcc caagagacgg cccgagacct tcgggagcgc
     2521 ctgaagcccg ttctggacgc cctggggccg ttgaatcggg atatgcaggc caaggccgcc
     2581 gcgatcatca aggccgtggg cgaaaagctg ctgacggaac agcgggaagt ccagcgccag
     2641 aaacaggccc agcgccagca ggaacgcggg cgcgcacatt tccccgaaaa gtgccacctg
     2701 acgtctaaga aaccattatt atcatgagcg gaacaaaact aaagcgccac aagggcgctt
     2761 tagtttgttt tcagtccagt tacgctggag tctgaggctc gtcatctgaa tcatgcgcgg
     2821 atgacacgaa ctcacgacgg cgatcacaga cattaaccca cagtacagac actgcgacaa
     2881 cgtggcaatt cgtcgcaata caacgctttg gcagtttatt cttgacatgt agtgaggggg
     2941 ctggtataat cacatagtac tgttatacag aaacagagga gatattacat atgagtaaag
     3001 gagaagagct tttcacagga gttgtcccaa tcctcgtgga attagacggt gatgttaatg
     3061 ggcacaagtt ctctgtcagt ggagagggtg aaggcgacgc aacatatggc aagctgaccc
     3121 ttaaatttat ttgcaccacg ggtaaactac ctgttccatg gccaacactg gtcactacgt
     3181 tcgggtatgg ggttcagtgc tttgcgcgct acccagatca catgaaacag cacgactttt
     3241 tcaagagtgc aatgcccgaa ggctatgtac aggagagaac catctttttt aaggatgacg
     3301 gcaactataa gacacgcgcc gaagtgaagt tcgagggtga tacccttgtt aatagaatcg
     3361 agttaaaggg tattgacttt aaggaagatg gaaatatttt aggccacaaa ctggaatata
     3421 actataactc ccataatgtg tacattatgg ccgacaagca aaagaacggt atcaaggtta
     3481 acttcaagat cagacacaac attgaggatg gaagcgttca actagccgac cattaccaac
     3541 aaaacacccc aattggcgat gggcctgtgc tgttaccaga caaccattac ctgtccactc
     3601 aatctgccct ttcgaaagat cccaacgaaa agcgcgacca catggtcctt cttgagtttg
     3661 tcacggctgc tgggattaca cacggcatgg atgaactata caaataaatc cgtaatcgtt
     3721 aatccgcaaa taacgtaaaa acccgcttcg gcgggttttt ttatgggggg agtttaggga
     3781 aagagcattt gtcagctgga aatctgctcg tcagtggcga gcggccgcga ttatcaaaaa
     3841 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     3901 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     3961 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     4021 gggagggctt accatctggc cccagtgctg caatgatacc gcgggaccca cgctcaccgg
     4081 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     4141 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     4201 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     4261 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     4321 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     4381 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     4441 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     4501 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     4561 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     4621 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     4681 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     4741 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     4801 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     4861 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgtcatga
     4921 ccaaaatccc ttaacgtg
//
