LOCUS       sequence-390637-       10167 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of BioID2-HA-13 x Linker- RBP(Z) on the cell 
            surface via a GPI anchor .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10167)
  AUTHORS   Gao SS, Shi R, Sun J, Tang Y, Zheng Z, Li JF, Li H, Zhang J, Leng Q,
            Xu J, Chen X, Zhao J, Sy MS, Feng L, Li C
  TITLE     GPI-anchored ligand-BioID2-tagging system identifies Galectin-1 
            mediating Zika virus entry.
  JOURNAL   iScience. 2022 Nov 2;25(12):105481. doi: 10.1016/j.isci.2022.105481.
            eCollection 2022 Dec 22.
  PUBMED    36404916
REFERENCE   2  (bases 1 to 10167)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2022-11-2"; volume: "25(12):105481. doi"; pages: " 
            10.1016/j.isci.2022.105481. eCollection 2022 Dec 22"
COMMENT     Sequence Label: pHAGE-PrP (1-22)-BioID2-HA-13 x Linker-
            RBP(Z)...
FEATURES             Location/Qualifiers
     source          1..10167
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2227..2530
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2531..2734
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2685..2705
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2731..2755
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2894..3589
                     /codon_start=1
                     /product="promiscuous R40G mutant of a biotin protein 
                     ligase from Aquifex aeolicus (Kim et al., 2016)"
                     /label=BioID2
                     /translation="FKNLIWLKEVDSTQERLKEWNVSYGTALVADRQTKGRGGLGRKWL
                     SQEGGLYFSFLLNPKEFENLLQLPLVLGLSVSEALEEITEIPFSLKWPNDVYFQEKKVS
                     GVLCELSKDKLIVGIGINVNQREIPEEIKDRATTLYEITGKDWDRKEVLLKVLKRISEN
                     LKKFKEKSFKEFKGKIESKMLYLGEEVKLLGEGKITGKLVGLSEKGGALILTEEGIKEI
                     LSGEFSLRRS"
     CDS             3590..3616
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4738..4749
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     misc_feature    5267..5834
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5284..5836
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5428..5445)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5655..5674
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5848..6543
                     /codon_start=1
                     /product="Zoanthus green fluorescent protein"
                     /label=ZsGreen1
                     /note="mammalian codon-optimized"
                     /translation="MAQSKHGLTKEMTMKYRMEGCVDGHKFVITGEGIGYPFKGKQAIN
                     LCVVEGGPLPFAEDILSAAFMYGNRVFTEYPQDIVDYFKNSCPAGYTWDRSFLFEDGAV
                     CICNADITVSVEENCMYHESKFYGVNFPADGPVMKKMTDNWEPSCEKIIPVPKQGILKG
                     DVSMYLLLKDGGRLRCQFDTVYKAKSVPRKMPDWHFIQHKLTREDRSDAKNQKWHLTEH
                     AIASGSALP"
     misc_feature    6557..7145
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6610..6630)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7028..7039)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7220..7453
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7572..7590)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7658..7762
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7763..7831,7832..8623)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7981..8000)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8794..9382
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9283..9302
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9536..9553
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9793..9814
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9829..9834,9835..9852,9853..9859)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9867..9883
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9872..9894
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9891..9907
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9891..9907
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9957..9976
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccaaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtgga
     2221 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     2281 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
     2341 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     2401 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
     2461 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     2521 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
     2581 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
     2641 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
     2701 gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg
     2761 gagacgccat ccacgctgtt ttgacctcca tagaagacac cggcggccgc accggtctgc
     2821 agctagcatg gcgaaccttg gctgctggat gctggttctc tttgtggcca catggagtga
     2881 cctgggcctc tgcttcaaga acctgatctg gctgaaggag gtggacagca cccaggagag
     2941 actgaaggag tggaacgtta gctacggcac cgccctggtg gccgacagac agaccaaggg
     3001 cagaggcggc ctgggcagaa agtggctgag ccaggagggc ggcctgtact tcagcttcct
     3061 gctgaacccc aaggagttcg agaacctgct gcagctgccc ctggtgctgg gcctgagcgt
     3121 gagcgaggcc ctggaggaga tcaccgagat ccccttcagc ctgaagtggc ccaacgacgt
     3181 gtacttccag gagaagaagg tgagcggcgt gctgtgcgag ctgagcaagg acaagctgat
     3241 cgtgggcatc ggcatcaacg tgaaccagag agagatcccc gaggagatca aggacagagc
     3301 caccaccctg tacgagatca ccggcaagga ctgggacaga aaggaggtgc tgctgaaggt
     3361 gctgaagaga atcagcgaga acctgaagaa gttcaaggag aagagcttca aggagttcaa
     3421 gggcaagatc gagagcaaga tgctgtacct gggcgaggag gtgaagctgc tgggcgaggg
     3481 caagatcacc ggcaagctgg tgggcctgag cgagaagggc ggcgccctga tcctgaccga
     3541 ggagggcatc aaggagatcc tgagcggcga gttcagcctg agaagaagct acccttacga
     3601 tgtaccggat tacgcagaat tcggatccgg tggaggcggg tctggaggcg ggggtagtgg
     3661 cgggggtgga agcgggggtg gaggcgggtc gggtggcgga ggtagcggag gcggtggaag
     3721 tggaggcggt ggaagtggtg gaggaggttc tggtggcgga ggtagcggtg gcggaggtag
     3781 cggaggcggt ggaagtggtg gcggaggtag cggaggcggt ggatcggcgc gccgttctag
     3841 aatcaggtgc attggagtca gcaataggga ctttgtggaa ggtatgtcag gtgggacttg
     3901 ggttgatgtt gtcttggaac atggaggttg tgtcaccgta atggcacagg acaaaccgac
     3961 tgtcgacata gagctggtta caacaacagt cagcaacatg gcggaggtaa gatcctactg
     4021 ctatgaggca tcaatatcgg acatggcttc ggacagccgc tgcccaacac aaggtgaagc
     4081 ctaccttgac aagcaatcag acactcaata tgtctgcaaa agaacgttag tggacagagg
     4141 ctggggaaat ggatgtggac tttttggcaa agggagcctg gtgacatgcg ctaagtttgc
     4201 atgctccaag aaaatgaccg ggaagagcat ccagccagag aatctggagt accggataat
     4261 gctgtcagtt catggctccc agcacagtgg gatgatcgtt aatgacacag gacatgaaac
     4321 tgatgagaat agagcgaagg ttgagataac gcccaattca ccaagagccg aagccaccct
     4381 ggggggtttt ggaagcctag gacttgattg tgaaccgagg acaggccttg acttttcaga
     4441 tttgtattac ttgactatga ataacaagca ctggttggtt cacaaggagt ggttccacga
     4501 cattccatta ccttggcacg ctggggcaga caccggaact ccacactgga acaacaaaga
     4561 agcactggta gagttcaagg acgcacatgc caaaaggcaa actgtcgtgg ttctagggag
     4621 tcaagaagga gcagttcaca cggcccttgc tggagctctg gaggctgaga tggatggtgc
     4681 aaagggaagg ctgtcctctg gccacttgaa atgtcgcctg aaaatggata aacttagatt
     4741 gaagggcgtg tcatactcct tgtgtaccgc agcgttcaca ttcaccaaga tcccggctga
     4801 aacactgcac gggacagtca cagtggaggt acagtacgca gggacagatg gaccttgcaa
     4861 ggttccagct cagatggcgg tggacatgca aactctgacc ccagttggga ggttgataac
     4921 cgctaacccc gtaatcactg aaagcactga gaactctaag atgatgctgg aacttgatcc
     4981 accatttggg gactcttaca ttgtcatagg agtcggggag aagaagatca cccaccactg
     5041 gcacaggagt ggcagcacca ttggaaaagc atttgaagcc actgtgagag gtgccaagag
     5101 aatggcagtc ttgggagaca cagcctggga ctttggatca tctagaggaa gtggaaccac
     5161 ttcaggtact acccgtcttc tatctgggca cacgtgtttc acgttgacag gtttgcttgg
     5221 gacgctagta accatgggct tgctgactta gctcgagtct agaggatccc tccccccccc
     5281 ctaacgttac tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc tatatgttat
     5341 tttccaccat attgccgtct tttggcaatg tgagggcccg gaaacctggc cctgtcttct
     5401 tgacgagcat tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg
     5461 tcgtgaagga agcagttcct ctggaagctt cttgaagaca aacaacgtct gtagcgaccc
     5521 tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa aagccacgtg
     5581 tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt tggatagttg
     5641 tggaaagagt caaatggctc tcctcaagcg tattcaacaa ggggctgaag gatgcccaga
     5701 aggtacccca ttgtatggga tctgatctgg ggcctcggtg cacatgcttt acatgtgttt
     5761 agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa
     5821 aaacacgatg ataatatggc cacacatatg gcccagtcca agcacggcct gaccaaggag
     5881 atgaccatga agtaccgcat ggagggctgc gtggacggcc acaagttcgt gatcaccggc
     5941 gagggcatcg gctacccctt caagggcaag caggccatca acctgtgcgt ggtggagggc
     6001 ggccccttgc ccttcgccga ggacatcttg tccgccgcct tcatgtacgg caaccgcgtg
     6061 ttcaccgagt acccccagga catcgtcgac tacttcaaga actcctgccc cgccggctac
     6121 acctgggacc gctccttcct gttcgaggac ggcgccgtgt gcatctgcaa cgccgacatc
     6181 accgtgagcg tggaggagaa ctgcatgtac cacgagtcca agttctacgg cgtgaacttc
     6241 cccgccgacg gccccgtgat gaagaagatg accgacaact gggagccctc ctgcgagaag
     6301 atcatccccg tgcccaagca gggcatcttg aagggcgacg tgagcatgta cctgctgctg
     6361 aaggacggtg gccgcttgcg ctgccagttc gacaccgtgt acaaggccaa gtccgtgccc
     6421 cgcaagatgc ccgactggca cttcatccag cacaagctga cccgcgagga ccgcagcgac
     6481 gccaagaacc agaagtggca cctgaccgag cacgccatcg cctccggctc cgccttgccc
     6541 tgaatcgata gatcctaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     6601 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     6661 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     6721 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     6781 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     6841 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     6901 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc
     6961 ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     7021 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     7081 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     7141 cccgcctgag atcctttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     7201 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt
     7261 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     7321 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     7381 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     7441 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag
     7501 aaatgaatat cagagagtga gaggcccggg ttaattaagg aaagggctag atcattcttg
     7561 aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
     7621 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     7681 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     7741 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     7801 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     7861 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     7921 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     7981 gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat
     8041 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     8101 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     8161 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     8221 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     8281 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     8341 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     8401 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     8461 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     8521 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     8581 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     8641 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     8701 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     8761 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     8821 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     8881 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     8941 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     9001 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     9061 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     9121 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     9181 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     9241 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     9301 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     9361 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     9421 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     9481 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     9541 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
     9601 gccgattcat taatgcagca agctcatggc tgactaattt tttttattta tgcagaggcc
     9661 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     9721 ggcttttgca aaaagctccc cgtggcacga caggtttccc gactggaaag cgggcagtga
     9781 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
     9841 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
     9901 ctatgacatg attacgaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     9961 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc aactggataa ctcaagctaa
    10021 ccaaaatcat cccaaacttc ccaccccata ccctattacc actgccaatt acctgtggtt
    10081 tcatttactc taaacctgtg attcctctga attattttca ttttaaagaa attgtatttg
    10141 ttaaatatgt actacaaact tagtagt
//
