LOCUS       sequence-390648-        4695 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Arabinose-induced expression of the photodegradable His-tagged 
            protein degFusionRed in E. coli..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4695)
  AUTHORS   Chernov KG, Manoilov KY, Oliinyk OS, Shcherbakova DM, Verkhusha VV
  TITLE     Photodegradable by Yellow-Orange Light degFusionRed Optogenetic 
            Module with Autocatalytically Formed Chromophore.
  JOURNAL   Int J Mol Sci. 2023 Mar 30;24(7):6526. doi: 10.3390/ijms24076526.
  PUBMED    37047499
REFERENCE   2  (bases 1 to 4695)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Int J Mol 
            Sci."; date: "2023-03-30"; volume: "24(7):6526. doi"; pages: " 
            10.3390/ijms24076526"
COMMENT     Sequence Label: pBAD-degFusionRed
FEATURES             Location/Qualifiers
     source          1..4695
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(59..145)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     298..315
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     CDS             complement(1066..1083)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        complement(1126..1410)
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     complement(1184..1203)
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     CDS             1437..2315
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     primer_bind     complement(2471..2493)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    2579..2719
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     2634..2653
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(2734..2751)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2905..3493)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2985..3004)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3664..4455,4456..4524))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4287..4306
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4525..4616)
                     /gene="bla"
                     /label=AmpR promoter
     terminator      complement(4635..4662)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gtcctgcccg ccaccctccg ggccgttgct tcgcaacgtt caaatccgct cccggcggat
       61 ttgtcctact caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt
      121 tcgactgagc ctttcgtttt atttgatgcc tggcagttcc ctactctcgc atggggagac
      181 cccacactac catcggcgct acggcgtttc acttctgagt tcggcatggg gtcaggtggg
      241 accaccgcgc tactgccgcc aggcaaattc tgttttatca gaccgcttct gcgttctgat
      301 ttaatctgta tcaggctgaa aatcttctct catccaccaa aacagccaag cttcgaattc
      361 ttatttccct ccatcgcctg ccccccctgt tgagtatctt gccactgcca cttcgtgctg
      421 ttccacatat gtctcatcgt cagcttcctt gatccgctcc agccgatgat cgacattata
      481 cacgccaggc atcttcaggt tagtggcggg cttcttgctc cggtaggtag ttcccagatt
      541 acagatcagg tgtcccccgc ccacgttttt cagagccatc cagcaccttc ccctcagttc
      601 tccgtctgct gcgtacataa gttcagttga gggcacccac ctcagggttt tcttctgcat
      661 gactgggcca ttggcaggga agttcactcc tctgaccttc acgttataga tcaggcagcc
      721 atcctggaga cttgtgtcct gtgtggcggt caggacgcct ccatcctcgt atgtggtcac
      781 tcgttcccag gtgaagccct cggggaaaga ctgtttaaag aagtcgggga tgccaggggg
      841 gtgcttgata aaagtgcggg acccatacat gaagctggtg gccagaatgt caaatgcgaa
      901 gggcagtggc ccgccttcga ccactttgat cctcatagtc tgtgtcccct cgtaaggctt
      961 cccttcgccc tcgcttgtgc atttgaagtg gtggttgttg acggttccct ccatatacag
     1021 tttcataggc atgttctcct taatcagctc gctcatagat ctgccatggt gatggtgatg
     1081 gtggctcccc atggttaatt cctcctgtta gcccaaaaaa cgggtatgga gaaacagtag
     1141 agagttgcga taaaaagcgt caggtaggat ccgctaatct tatggataaa aatgctatgg
     1201 catagcaaag tgtgacgccg tgcaaataat caatgtggac ttttctgccg tgattataga
     1261 cacttttgtt acgcgttttt gtcatggctt tggtcccgct ttgttacaga atgcttttaa
     1321 taagcggggt taccggtttg gttagcgaga agagccagta aaagacgcag tgacggcaat
     1381 gtctgatgca atatggacaa ttggtttctt ctctgaatgg cgggagtatg aaaagtatgg
     1441 ctgaagcgca aaatgatccc ctgctgccgg gatactcgtt taatgcccat ctggtggcgg
     1501 gtttaacgcc gattgaggcc aacggttatc tcgatttttt tatcgaccga ccgctgggaa
     1561 tgaaaggtta tattctcaat ctcaccattc gcggtcaggg ggtggtgaaa aatcagggac
     1621 gagaatttgt ttgccgaccg ggtgatattt tgctgttccc gccaggagag attcatcact
     1681 acggtcgtca tccggaggct cgcgaatggt atcaccagtg ggtttacttt cgtccgcgcg
     1741 cctactggca tgaatggctt aactggccgt caatatttgc caatacgggg ttctttcgcc
     1801 cggatgaagc gcaccagccg catttcagcg acctgtttgg gcaaatcatt aacgccgggc
     1861 aaggggaagg gcgctattcg gagctgctgg cgataaatct gcttgagcaa ttgttactgc
     1921 ggcgcatgga agcgattaac gagtcgctcc atccaccgat ggataatcgg gtacgcgagg
     1981 cttgtcagta catcagcgat cacctggcag acagcaattt tgatatcgcc agcgtcgcac
     2041 agcatgtttg cttgtcgccg tcgcgtctgt cacatctttt ccgccagcag ttagggatta
     2101 gcgtcttaag ctggcgcgag gaccaacgta tcagccaggc gaagctgctt ttgagcacca
     2161 cccggatgcc tatcgccacc gtcggtcgca atgttggttt tgacgatcaa ctctatttct
     2221 cgcgggtatt taaaaaatgc accggggcca gcccgagcga gttccgtgcc ggttgtgaag
     2281 aaaaagtgaa tgatgtagcc gtcaagttgt cataattggt aacgaatcag acaattgacg
     2341 gcttgacgga gtagcatagg gtttgcagaa tccctgcttc gtccatttga caggcacatt
     2401 atgcatgccg cttcgccttc gcgcgcgaat tgatctgctg cctcgcgcgt ttcggtgatg
     2461 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg
     2521 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg
     2581 cagccatgac ccagtcacgt agcgatagcg gagtgtatac tggcttaact atgcggcatc
     2641 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag
     2701 gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     2761 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     2821 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     2881 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     2941 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     3001 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     3061 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     3121 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     3181 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     3241 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     3301 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
     3361 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     3421 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     3481 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     3541 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     3601 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     3661 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     3721 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     3781 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     3841 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     3901 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     3961 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     4021 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     4081 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     4141 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     4201 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     4261 ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca catagcagaa ctttaaaagt
     4321 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     4381 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     4441 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     4501 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     4561 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaaagag
     4621 tttgtagaaa cgcaaaaagg ccatccgtca ggatggcctt ctgcttaatt tgatgcctgg
     4681 cagtttatgg cgggc
//
