LOCUS       sequence-390724-       10861 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral plasmid encoding SYT9 with D197, 199N mutations followed 
            by an internal ribosomal entry site followed by EGFP under the human
            synapsin promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10861)
  AUTHORS   Seibert MJ, Evans CS, Stanley KS, Wu Z, Chapman ER
  TITLE     Synaptotagmin 9 Modulates Spontaneous Neurotransmitter Release in 
            Striatal Neurons by Regulating Substance P Secretion.
  JOURNAL   J Neurosci. 2023 Mar 1;43(9):1475-1491. doi: 
            10.1523/JNEUROSCI.1857-22.2023. Epub 2023 Feb 2.
  PUBMED    36732068
REFERENCE   2  (bases 1 to 10861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1523/JNEUROSCI.1857-22.2023"; journalName: "J Neurosci"; date: 
            "2023-03-1- 1"; volume: "43"; issue: "9"; pages: "1475-1491"
COMMENT     Sequence Label: pFSynW SYT9 D197, 199N IRES GFP
FEATURES             Location/Qualifiers
     source          1..10861
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(82..102)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        87..416
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      267..402
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     329..348
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        464..511
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             530..904
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    1034..1155
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1071..1090)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1125..1144
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1204..1220)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1204..1220)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1217..1239)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1228..1244
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1252..1258,1259..1276,1277..1282))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1297..1318
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1435..1452)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1606..2194)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1686..1705)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2365..3156,3157..3225))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2988..3007
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3226..3330)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3405..3424)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3596..3975
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3977..4175
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4130..4150
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             4193..4373
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4420..4545
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5038..5271
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5456..5500
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5798..5915
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5960..6406
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     misc_feature    7595..8166
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    7616..8168
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7760..7777)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7987..8006
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(8168..8170,8171..8173,8174..8887)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(8168..8170,8171..8173,8174..8884)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8213..8234)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8474..8493)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8821..8842
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    8911..9499
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8964..8984)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9382..9393)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(9502..9518)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9503..9519)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             10024..10204
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(10230..10247)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10236..10460
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      join(10506..10861,1..73)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10593..10612)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10803..10824
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
       61 tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct
      121 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa
      181 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
      241 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
      301 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct
      361 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
      421 tcccgggagc ttgtatatcc attttcggat ctgatcagca cgtgttgaca attaatcatc
      481 ggcatagtat atcggcatag tataatacga caaggtgagg aactaaacca tggccaagtt
      541 gaccagtgcc gttccggtgc tcaccgcgcg cgacgtcgcc ggagcggtcg agttctggac
      601 cgaccggctc gggttctccc gggacttcgt ggaggacgac ttcgccggtg tggtccggga
      661 cgacgtgacc ctgttcatca gcgcggtcca ggaccaggtg gtgccggaca acaccctggc
      721 ctgggtgtgg gtgcgcggcc tggacgagct gtacgccgag tggtcggagg tcgtgtccac
      781 gaacttccgg gacgcctccg ggccggccat gaccgagatc ggcgagcagc cgtgggggcg
      841 ggagttcgcc ctgcgcgacc cggccggcaa ctgcgtgcac ttcgtggccg aggagcagga
      901 ctgacacgtg ctacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg
      961 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt
     1021 cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     1081 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     1141 catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc
     1201 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     1261 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     1321 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     1381 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     1441 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     1501 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     1561 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     1621 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     1681 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     1741 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     1801 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     1861 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     1921 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     1981 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     2041 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     2101 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     2161 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     2221 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     2281 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     2341 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     2401 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     2461 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     2521 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     2581 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     2641 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     2701 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     2761 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     2821 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     2881 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     2941 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     3001 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     3061 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     3121 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     3181 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     3241 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     3301 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     3361 cgacggatcg ggagatctcc cgatccccta tggtgcactc tcagtacaat ctgctctgat
     3421 gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc
     3481 gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg
     3541 cttagggtta ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgttgacat
     3601 tgattattga ctagttatta atagtaatca attacggggt cattagttca tagcccatat
     3661 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
     3721 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
     3781 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
     3841 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
     3901 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
     3961 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt
     4021 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac
     4081 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc
     4141 ggtaggcgtg tacggtggga ggtctatata agcagcgcgt tttgcctgta ctgggtctct
     4201 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     4261 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     4321 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
     4381 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
     4441 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
     4501 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
     4561 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
     4621 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
     4681 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
     4741 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
     4801 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
     4861 agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag
     4921 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     4981 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     5041 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
     5101 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     5161 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     5221 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     5281 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     5341 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     5401 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     5461 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     5521 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     5581 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     5641 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
     5701 aggtggagag agagacagag acagatccat tcgattagtg aacggatcgg cactgcgtgc
     5761 gccaattctg cagacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg
     5821 gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag
     5881 aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag
     5941 atccagtttg gttaattaaa gtgcaagtgg ttttaggacc aggatgaggc ggggtggggg
     6001 tgcctacctg acgaccgacc ccgacccact ggacaagcac ccaaccccca ttccccaaat
     6061 tgcgcatccc ctatcagaga gggggagggg aaacaggatg cggcgaggcg cgtgcgcact
     6121 gccagcttca gcaccgcgga cagtgccttc gcccccgcct ggcggcgcgc gccaccgccg
     6181 cctcagcact gaaggcgcgc tgacgtcact cgccggtccc ccgtaaactc cccttcccgg
     6241 ccaccttggt cgcgtccgcg ccgccgccgg cccagccgga ccgcaccacg cgaggcgcga
     6301 gatagggggg cacgggcgcg accatctgcg ctgcggcgcc ggcgactcag cgctgcctca
     6361 gtctgcggtg ggcagcggag gagtcgtgtc gtgcctgaga gcgcaggtac ggctagcgga
     6421 tccatgttcc cggaaccccc gaccctgggg tctccagcgc ctaagacacc tccagactcc
     6481 agccgcatca ggcagggcgc agtgcctgcc tgggtcctgg ctaccatcgt gctgggctcg
     6541 ggcctcctgg tcttcagcag ttgtttctgt ctctaccgga agcgctgtcg gagacggatg
     6601 ggcaaaaaga gtcaggccca agcccaagtc catcttcagg aagtgaagga gctgggccgg
     6661 agttacatag ataaggttca gcctgaaata gaggagctgg accggtcacc atccatgcct
     6721 ggacagcagg tatcagacaa acaccagcta ggccgactgc agtattcact ggactatgac
     6781 ttccagactg gccagctcct ggtgggcatc ttgcaagctc agggactggc tgcgttggac
     6841 ctaggaggtt cctcagatcc ctatgttagt gtctatctgc tgccagacaa gcggaggcga
     6901 catgagacca aggtgcatcg gcagaccctg aatccacact ttggagagac atttgccttc
     6961 aaggtcccat acgtggaact agggggcaga gtgctggtca tggcggtgta taactttaat
     7021 cggttctccc gcaacgatgc catcggggag gtgcgggtgc ctatgagttc agtgaacctg
     7081 gggcggccgg tgcaggcctg gagagagctg caggtggctc ccaaagagga gcaagagaaa
     7141 ctgggggaca tctgcttctc tctccgttat gtccccacgg ccgggaagct caccgtcatt
     7201 gtcttggaag ctaaaaacct gaagaaaatg gatgtaggag gactctcaga tccctatgtg
     7261 aaggtgcacc tgctccaggg aggtaaaaag gttcgcaaga agaaaaccac cattaagaag
     7321 aacaccctga acccctatta caacgaggcc ttcagctttg aggtgccctg tgaccaggtg
     7381 cagaaagtcc aggtggagct gactgttctg gattatgaca aactggggaa gaatgaggcc
     7441 atcgggagag tggcggtggg cgcagcagtc ggtggggctg gcctgcggca ctgggctgac
     7501 atgttggcca accccaggcg ccccattgcc cagtggcact ctctgcggcc ccctgacaga
     7561 gccaggccaa taccagcacc ctaatctaga attccccctc tccctccccc ccctaacgtt
     7621 actggccgaa gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc
     7681 atattgccgt cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc
     7741 attcctaggg gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag
     7801 gaagcagttc ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg
     7861 cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat
     7921 acacctgcaa aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga
     7981 gtcaaatggc tctcctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc
     8041 cattgtatgg gatctgatct ggggcctcgg tacacatgct ttacatgtgt ttagtcgagg
     8101 ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga
     8161 tgataatatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
     8221 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
     8281 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
     8341 gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca
     8401 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
     8461 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
     8521 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
     8581 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca
     8641 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca
     8701 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
     8761 caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca
     8821 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
     8881 caagtaaaat tcgatatcaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa
     8941 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     9001 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     9061 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     9121 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     9181 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     9241 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     9301 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg
     9361 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     9421 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     9481 ctttgggccg cctccccgca tcgataccgt cgacctcgag acctagaaaa acatggagca
     9541 atcacaagta gcaatacagc agctaccaat gctgattgtg cctggctaga agcacaagag
     9601 gaggaggagg tgggttttcc agtcacacct caggtacctt taagaccaat gacttacaag
     9661 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
     9721 tcccaacgaa gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct
     9781 gattggcaga actacacacc agggccaggg atcagatatc cactgacctt tggatggtgc
     9841 tacaagctag taccagttga gcaagagaag gtagaagaag ccaatgaagg agagaacacc
     9901 cgcttgttac accctgtgag cctgcatggg atggatgacc cggagagaga agtattagag
     9961 tggaggtttg acagccgcct agcatttcat cacatggccc gagagctgca tccggactgt
    10021 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
    10081 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
    10141 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
    10201 agcagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
    10261 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
    10321 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
    10381 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
    10441 ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta
    10501 tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
    10561 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
    10621 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
    10681 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
    10741 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
    10801 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
    10861 t
//
