LOCUS       sequence-390733-        8288 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For in vitro transcription of heiCas9 mRNA - mammalian codon 
            optimized Cas9, N-terminal hei-tag (Myc-flexible 
            linker-optimizedNLS) and C-terminal oNLS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8288)
  AUTHORS   Thumberger T, Tavhelidse-Suck T, Gutierrez-Triana JA, Cornean A, 
            Medert R, Welz B, Freichel M, Wittbrodt J
  TITLE     Boosting targeted genome editing using the hei-tag.
  JOURNAL   Elife. 2022 Mar 25;11. pii: 70558. doi: 10.7554/eLife.70558.
  PUBMED    35333175
REFERENCE   2  (bases 1 to 8288)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2022-03-25"; volume: "11. pii: 70558. doi"; pages: " 
            10.7554/eLife.70558"
COMMENT     Sequence Label: pCS2+ heiCas9
FEATURES             Location/Qualifiers
     source          1..8288
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    29..163
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(53..72)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     107..126
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(276..296)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(276..294)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(315..331)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(315..331)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(328..350)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    339..355
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(363..369,370..387,388..393))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    408..429
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(546..563)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(717..1305)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(797..816)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1476..2267,2268..2336))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2099..2118
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2337..2441)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2467..2922)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2604..2625)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2816..2835
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        2993..3977
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
     promoter        4012..4030
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     4012..4029
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             4076..4105
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4151..8251
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             4151..8251
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
ORIGIN
        1 ctagaactat agtgagtcgt attacgtaga tccagacatg ataagataca ttgatgagtt
       61 tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc
      121 tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat
      181 tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaattcg cggccgcggc
      241 gccaatgcat tgggcccggt acccagcttt tgttcccttt agtgagggtt aattgcgcgc
      301 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
      361 cacaacatac gagccgggag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
      421 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
      481 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
      541 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
      601 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
      661 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
      721 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
      781 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
      841 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
      901 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
      961 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     1021 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     1081 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     1141 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     1201 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     1261 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     1321 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     1381 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     1441 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     1501 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     1561 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     1621 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     1681 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     1741 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     1801 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     1861 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     1921 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     1981 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     2041 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     2101 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     2161 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     2221 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     2281 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     2341 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     2401 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     2461 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
     2521 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
     2581 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
     2641 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
     2701 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
     2761 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
     2821 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
     2881 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg ccattcaggc
     2941 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagtcgaccg
     3001 ccaattcaat atggcgtata tggactcatg ccaattcaat atggtggatc tggacctgtg
     3061 ccaattcaat atggcgtata tggactcgtg ccaattcaat atggtggatc tggaccccag
     3121 ccaattcaat atggcggact tggcaccatg ccaattcaat atggcggacc tggcactgtg
     3181 ccaactgggg aggggtctac ttggcacggt gccaagtttg aggaggggtc ttggccctgt
     3241 gccaagtccg ccatattgaa ttggcatggt gccaataatg gcggccatat tggctatatg
     3301 ccaggatcaa tatataggca atatccaata tggccctatg ccaatatggc tattggccag
     3361 gttcaatact atgtattggc cctatgccat atagtattcc atatatgggt tttcctattg
     3421 acgtagatag cccctcccaa tgggcggtcc catataccat atatggggct tcctaatacc
     3481 gcccatagcc actcccccat tgacgtcaat ggtctctata tatggtcttt cctattgacg
     3541 tcatatgggc ggtcctattg acgtatatgg cgcctccccc attgacgtca attacggtaa
     3601 atggcccgcc tggctcaatg cccattgacg tcaataggac cacccaccat tgacgtcaat
     3661 gggatggctc attgcccatt catatccgtt ctcacgcccc ctattgacgt caatgacggt
     3721 aaatggccca cttggcagta catcaatatc tattaatagt aacttggcaa gtacattact
     3781 attggaagta cgccagggta cattggcagt actcccattg acgtcaatgg cggtaaatgg
     3841 cccgcgatgg ctgccaagta catccccatt gacgtcaatg gggaggggca atgacgcaaa
     3901 tgggcgttcc attgacgtaa atgggcggta ggcgtgccta atgggaggtc tatataagca
     3961 atgctcgttt agggaaccgc cattctgcct ggggacgtcg gagcaagctt gatttaggtg
     4021 acactataga atacaagcta cttgttcttt ttgcaggatc caccggttta ccatggagca
     4081 gaagctgatc agcgaggagg acctgggagg aagcggacca cctcccaaga ggcccaggct
     4141 ggacctcgag gataaaaagt attctattgg tttagacatc ggcaccaaca gcgtgggctg
     4201 ggccgtgatc accgacgagt acaaggtgcc cagcaagaaa ttcaaggtgc tgggcaacac
     4261 cgacagacac agcatcaaga aaaacctgat cggcgccctg ctcttcgact ccggcgaaac
     4321 cgccgaggcc accagactga agagaaccgc cagacggaga tacaccagac ggaagaatag
     4381 aatctgctac ctgcaggaga tcttcagcaa cgagatggcc aaggtggacg atagcttctt
     4441 tcacagactg gaagagagct tcctggtgga agaggacaag aaacacgaga gacaccccat
     4501 cttcggcaac atcgtggacg aggtggccta ccacgagaag taccccacca tctaccacct
     4561 gagaaagaaa ctggtggaca gcaccgacaa ggccgacctg agactgatct acctggcact
     4621 ggcccacatg atcaagttca gaggccactt cctgatcgag ggcgacctga accccgacaa
     4681 cagcgacgtg gacaagctgt tcatccagct ggtgcagacc tacaaccagc tgttcgaaga
     4741 gaaccctatc aacgccagcg gcgtggacgc caaggccatc ctgagcgcca gactcagcaa
     4801 gagcagacgg ctggagaacc tgatcgccca gctgcccggc gagaagaaaa acggcctgtt
     4861 cggcaacctg atcgccctga gcctgggcct gacccccaac ttcaagagca acttcgacct
     4921 ggccgaggac gccaagctgc agctgagcaa ggacacctac gacgatgacc tggacaacct
     4981 cctggcccag atcggcgacc agtacgccga cctgttcctc gcagccaaga acctgagcga
     5041 cgccatcctc ctgagcgaca tcctcagagt gaacaccgag atcaccaagg ctcccctgag
     5101 cgccagcatg atcaagagat acgacgagca ccatcaggac ctgaccctcc tgaaggccct
     5161 cgtgagacaa cagctgcccg agaagtacaa ggagatcttc tttgaccaga gcaagaacgg
     5221 ctacgccggc tacatcgacg gaggcgccag tcaggaagag ttctacaagt tcatcaagcc
     5281 catcctggag aagatggacg gcaccgaaga gctgctcgtg aagctgaaca gagaggacct
     5341 gctcagaaag cagagaacct tcgacaacgg cagcatcccc caccagatcc acctgggcga
     5401 gctgcacgcc atcctgagac ggcaggagga cttctacccc ttcctgaagg acaacagaga
     5461 gaagattgaa aagatcctga ccttcagaat cccctactat gtgggccccc tggccagagg
     5521 caacagcaga ttcgcctgga tgaccaggaa gagcgaagag acaatcacac cttggaactt
     5581 cgaagaggtg gtcgacaaag gcgccagcgc ccagagcttc atcgagagaa tgaccaactt
     5641 cgacaagaac ctgcccaacg agaaggtgct gcccaagcac agcctcctgt acgagtactt
     5701 caccgtgtac aacgagctga ccaaggtgaa gtacgtgacc gagggcatga gaaagcctgc
     5761 ctttctgagt ggcgagcaga agaaagccat cgtggacctg ctcttcaaga ccaacagaaa
     5821 agtgaccgtg aagcagctga aggaggacta cttcaagaaa atcgagtgct tcgacagcgt
     5881 ggagatcagc ggcgtggagg acagattcaa cgccagcctg ggcacctacc acgacctgct
     5941 caagattatc aaagacaagg acttcctgga caacgaagag aacgaggaca tcctggagga
     6001 catcgtgctg acactgaccc tcttcgagga cagagagatg atcgaagaga gactgaagac
     6061 ctacgcccac ctgttcgatg acaaggtgat gaagcagctg aagagacgga gatacaccgg
     6121 ctggggcaga ctgagcagaa agctgatcaa cggcatcaga gacaagcaga gcggcaagac
     6181 catcctggac ttcctgaaga gcgacggctt cgccaacaga aacttcatgc agctgatcca
     6241 cgatgacagc ctgaccttca aggaggacat ccagaaagcc caagtgagcg ggcagggcga
     6301 cagcctgcac gagcatatcg ccaacctggc tggcagcccc gccatcaaga aaggcatcct
     6361 gcagaccgtg aaggtcgtgg acgagctggt caaggtgatg ggcagacaca agcccgagaa
     6421 catcgtgatt gagatggcca gagagaacca gacaacccag aagggccaga agaacagcag
     6481 agagagaatg aagagaatcg aagagggcat caaggagctg ggcagccaga tcctgaagga
     6541 gcaccccgtg gagaacaccc agctgcagaa cgagaagctg tacctgtatt acctgcagaa
     6601 cggcagagac atgtacgtgg accaggagct ggacatcaac agactgagcg attacgacgt
     6661 ggatcacatc gtcccccaga gcttcctgaa ggatgacagc atcgataaca aggtgctgac
     6721 cagaagcgac aagaacagag gcaagagcga caacgtgccc agcgaagagg tcgtgaagaa
     6781 aatgaagaac tactggagac agctcctgaa cgccaagctg atcacccaga gaaagttcga
     6841 caacctgacc aaggccgaga gaggcgggct gagcgagctc gacaaagccg gcttcatcaa
     6901 gagacagctg gtggaaacca gacagatcac caagcacgtg gcccagatcc tggacagcag
     6961 aatgaacacc aagtacgacg agaacgataa gctgatcaga gaggtgaagg tcatcaccct
     7021 gaagagcaaa ctggtgagcg acttcagaaa ggacttccag ttctacaagg tgagagagat
     7081 caataactac catcacgctc atgacgccta cctgaacgcc gtcgtgggca ccgccctgat
     7141 caagaaatac cccaagctgg agagcgagtt cgtgtacggc gactacaagg tgtacgacgt
     7201 gagaaagatg atcgccaaga gcgagcagga gatcggcaag gccaccgcca agtacttctt
     7261 ttacagcaac atcatgaact tctttaagac cgagatcacc ctggccaacg gcgagatcag
     7321 aaagaggccc ctgatcgaaa ccaacggcga aaccggcgag atcgtgtggg acaagggcag
     7381 agacttcgcc accgtgagaa aggtgctgag catgccccag gtgaacatcg tgaagaaaac
     7441 cgaggtgcag accggaggct tcagcaagga gagcatcctg cccaagagaa acagcgacaa
     7501 gctgatcgcc agaaagaaag actgggaccc caagaaatac ggaggcttcg acagccccac
     7561 cgtggcctac agcgtgctgg tcgtggccaa ggtggagaag ggcaagagca agaaactgaa
     7621 gagcgtgaag gagctcctgg gcatcaccat catggagaga tccagcttcg agaagaaccc
     7681 catcgacttc ctggaggcca agggctacaa ggaggtgaag aaagacctga ttatcaagct
     7741 gcccaagtac agcctgttcg agctggagaa cggcagaaag agaatgctgg ccagcgccgg
     7801 cgagctgcag aagggcaacg agctggccct gcccagcaag tacgtgaact tcctgtacct
     7861 ggccagccac tacgagaagc tgaagggcag ccccgaggac aacgagcaga agcagctgtt
     7921 cgtggagcag cacaagcact acctggacga aatcattgag cagatcagcg agttcagtaa
     7981 gagagtgatc ctggctgacg ccaacctgga caaggtgctg agcgcctaca acaagcacag
     8041 agacaagccc atcagagagc aggccgagaa catcattcac ctgttcaccc tgaccaacct
     8101 gggcgcaccc gcagccttca agtacttcga caccacaatc gacagaaaga gatacaccag
     8161 caccaaggag gtgctggacg ccaccctgat ccaccagagc atcaccggcc tgtacgaaac
     8221 cagaatcgac ctgtcacagc ttgggggtga cggatcccct cctcccaaga ggcccaggct
     8281 ggactaat
//
