LOCUS       sequence-39074-e         810 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Vick JE, Johnson ET, Choudhary S, Bloch SE, Lopez-Gallego F, 
            Srivastava P, Tikh IB, Wawrzyn GT, Schmidt-Dannert C
  TITLE     Optimized compatible set of BioBrick vectors for metabolic pathway 
            engineering.
  JOURNAL   Appl Microbiol Biotechnol. 2011 Dec;92(6):1275-86. Epub 2011 Oct 28.
  PUBMED    22033566
REFERENCE   2  (bases 1 to 810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Microbiol Biotechnol."; date: "2011-12"; volume: "92(6)"; pages: 
            "1275-86. Epub 2011 Oct 28"
COMMENT     Sequence Label: pUCBB-lacZa
FEATURES             Location/Qualifiers
     source          1..810
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(20..37)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     regulatory      47..56
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     67..86
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     280..302
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     294..311
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     295..311
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    312..368
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     promoter        complement(join(431..437,438..455,456..461))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    476..497
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      559..602
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(606..624)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        692..796
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 caanatgcgt aggagatcag tgatggtgat ggtgatgctc gaggcggccg ccatggatgc
       61 attatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc
      121 acagatgcgt aaggagaaaa taccgcatca ggcgccattc gccattcagg ctgcgcaact
      181 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat
      241 gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa
      301 cgacggccag tgaattcgag ctcggtaccc ggggatcctc tagagtcgac ctgcaggcat
      361 gcaagcttgg cgtaatcatg gtcatatgga tccagatctc ctccttctac tagacgctca
      421 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
      481 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggtctagata
      541 agttcaggat gaattccaga aatcatcctt agcgaaagct aaggattttt tttatctgaa
      601 attctgcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
      661 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
      721 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
      781 ttgaaaaagg aagagtatga gtattcaaca
//
