LOCUS       sequence-390762-        8322 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lentiviral stable expression of mCert1 gRNA 2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8322)
  AUTHORS   Dhekne HS, Tonelli F, Yeshaw WM, Chiang CY, Limouse C, Jaimon E, 
            Purlyte E, Alessi DR, Pfeffer SR
  TITLE     Genome-wide screen reveals Rab12 GTPase as a critical activator of 
            Parkinson's disease-linked LRRK2 kinase.
  JOURNAL   Elife. 2023 Oct 24;12:e87098. doi: 10.7554/eLife.87098.
  PUBMED    37874635
REFERENCE   2  (bases 1 to 8322)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2023-10-24"; volume: "12:e87098. doi"; pages: " 
            10.7554/eLife.87098"
COMMENT     Sequence Label: Lenti-guide-puro mCert1-2
FEATURES             Location/Qualifiers
     source          1..8322
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        162..410
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     162..182
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     333..352
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        431..506
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    562..679
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        818..1996
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          1049..1987
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1944..1964
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             2009..2608
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2009..2028)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2505..2525
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2624..3212
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2677..2697)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3095..3106)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3284..3517
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3595..3716
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3632..3651)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3686..3705
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3756..3891
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3911..3930)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3912..3930)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3940..3957)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3940..3956)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3949..3971)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4098..4553
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4185..4204)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4395..4416
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4579..4683
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4684..4752,4753..5544)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4902..4921)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5715..6303
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6204..6223
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6457..6474
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6591..6612
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6627..6632,6633..6650,6651..6657)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6665..6681
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6670..6692
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6689..6705
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6689..6705
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6724..6744
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6726..6744
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6772..6998
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6999..7179
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7226..7351
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7844..8077
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8262..8306
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt
       61 taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat
      121 tatcgtttca gacccacctc ccaaccccga ggggacccag agagggccta tttcccatga
      181 ttccttcata tttgcatata cgatacaagg ctgttagaga gataattaga attaatttga
      241 ctgtaaacac aaagatatta gtacaaaata cgtgacgtag aaagtaataa tttcttgggt
      301 agtttgcagt tttaaaatta tgttttaaaa tggactatca tatgcttacc gtaacttgaa
      361 agtatttcga tttcttggct ttatatatct tgtggaaagg acgaaacacc gccagtttct
      421 cacgtaaact ggtttagagc tagaaatagc aagttaaaat aaggctagtc cgttatcaac
      481 ttgaaaaagt ggcaccgagt cggtgctttt ttaagcttgg cgtaactaga tcttgagaca
      541 aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg
      601 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
      661 tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccac tttggcgccg
      721 gctcgagggg gcccgggtgc aaagatggat aaagttttaa acagagagga atctttgcag
      781 ctaatggacc ttctaggtct tgaaaggagt gggaattggc tccggtgccc gtcagtgggc
      841 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgatccgg
      901 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
      961 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
     1021 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg
     1081 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt
     1141 gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta
     1201 aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt
     1261 gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta
     1321 aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg
     1381 ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc
     1441 gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg
     1501 ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc
     1561 cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc
     1621 gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc
     1681 gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga
     1741 ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac
     1801 gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt
     1861 ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt
     1921 tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc
     1981 atttcaggtg tcgtgacgta cggccaccat gaccgagtac aagcccacgg tgcgcctcgc
     2041 cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc
     2101 cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga
     2161 actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc
     2221 cgccgtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat
     2281 cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg
     2341 cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggagtctc
     2401 gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc
     2461 cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta
     2521 cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg
     2581 gtgcatgacc cgcaagcccg gtgcctgaac gcgttaagtc gacaatcaac ctctggatta
     2641 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
     2701 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
     2761 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
     2821 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
     2881 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
     2941 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
     3001 cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg
     3061 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
     3121 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac
     3181 gagtcggatc tccctttggg ccgcctcccc gcgtcgactt taagaccaat gacttacaag
     3241 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
     3301 tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc
     3361 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     3421 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     3481 ctcagaccct tttagtcagt gtggaaaatc tctagcagta cgtatagtag ttcatgtcat
     3541 cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg
     3601 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     3661 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     3721 gtctggctct agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     3781 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     3841 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagggacgt
     3901 acccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg
     3961 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt
     4021 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
     4081 cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     4141 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     4201 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     4261 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
     4321 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     4381 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
     4441 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
     4501 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc
     4561 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     4621 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     4681 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     4741 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     4801 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     4861 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     4921 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     4981 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     5041 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     5101 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     5161 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     5221 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     5281 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     5341 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     5401 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     5461 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     5521 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     5581 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     5641 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     5701 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     5761 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     5821 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     5881 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     5941 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     6001 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     6061 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     6121 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     6181 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     6241 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     6301 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     6361 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     6421 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     6481 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     6541 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
     6601 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
     6661 ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag
     6721 cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctta atgtagtctt
     6781 atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat gccttacaag
     6841 gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat cgtgccttat
     6901 taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg ccgcattgca
     6961 gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg ttagaccaga
     7021 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     7081 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     7141 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt
     7201 gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg
     7261 cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg
     7321 ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg
     7381 atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt
     7441 atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga
     7501 aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat cagaagaact
     7561 tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa
     7621 agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc
     7681 acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa
     7741 gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg
     7801 caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg
     7861 ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg
     7921 ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg
     7981 cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc
     8041 tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa
     8101 aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac
     8161 agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct
     8221 taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat
     8281 tggaattaga taaatgggca agtttgtgga attggtttaa ca
//
