LOCUS       sequence-390791-        9417 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of full length HSF2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9417)
  AUTHORS   Price RM, Budzynski MA, Shen J, Mitchell JE, Kwan JZJ, Teves SS
  TITLE     Heat shock transcription factors demonstrate a distinct mode of 
            interaction with mitotic chromosomes.
  JOURNAL   Nucleic Acids Res. 2023 Apr 28:gkad304. doi: 10.1093/nar/gkad304.
  PUBMED    37114996
REFERENCE   2  (bases 1 to 9417)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2023 Apr 28:gkad304. doi: 10.1093/nar/gkad304."
COMMENT     Sequence Label: Halo-HSF2
FEATURES             Location/Qualifiers
     source          1..9417
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     481..500
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(719..823)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(849..1304)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(986..1007)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1198..1217
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1431..1453
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1445..1462
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1446..1462
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   1592..1654
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        2107..2318
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             2331..2599
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     regulatory      2625..2634
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2634..2699
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             2709..3599
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
                     YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
                     HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII
                     DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALV
                     EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDL
                     IGSEIARWLSTLEISG"
     CDS             3612..3632
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="EDLYFQS"
     misc_feature    5208..5780
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5375..5392)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5602..5621
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5789..6583
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLAATGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5843..5862)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6453..6472
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6643..6663)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    6705..6826
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6742..6761)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6796..6815
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   7589..7623
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(8114..8130)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8114..8130)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8127..8149)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8138..8154
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8162..8168,8169..8186,8187..8192))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8207..8228
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8345..8362)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8516..9104)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8596..8615)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9275..9417,1..649,650..718))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
       61 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
      121 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
      181 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
      241 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
      301 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
      361 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
      421 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
      481 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
      541 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
      601 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
      661 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
      721 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
      781 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
      841 tgccacctaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat
      901 cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata
      961 gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt
     1021 ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc
     1081 atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa
     1141 agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg
     1201 gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt
     1261 aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcccatt cgccattcag
     1321 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc
     1381 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg
     1441 acgttgtaaa acgacggcca gtgagcgcgc ctcgttcatt cacgtttttg aacccgtgga
     1501 ggacgggcag actcgcggtg caaatgtgtt ttacagcgtg atggagcaga tgaagatgct
     1561 cgacacgctg cagaacacgc agctagatta accctagaaa gataatcata ttgtgacgta
     1621 cgttaaagat aatcatgtgt aaaattgacg catgtgtttt atcggtctgt atatcgaggt
     1681 ttatttatta atttgaatag atattaagtt ttattatatt tacacttaca tactaataat
     1741 aaattcaaca aacaatttat ttatgtttat ttatttatta aaaaaaacaa aaactcaaaa
     1801 tttcttctat aaagtaacaa aacttttatg agggacagcc cccccccaaa gcccccaggg
     1861 atgtaattac gtccctcccc cgctaggggg cagcagcgag ccgcccgggg ctccgctccg
     1921 gtccggcgct ccccccgcat ccccgagccg gcagcgtgcg gggacagccc gggcacgggg
     1981 aaggtggcac gggatcgctt tcctctgaac gcttctcgct gctctttgag cctgcagaca
     2041 cctgggggga tacggggaaa aggcctccac ggccaaggat ctgcgatcgc tccggtgccc
     2101 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
     2161 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
     2221 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
     2281 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca
     2341 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg
     2401 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa
     2461 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc
     2521 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg
     2581 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac tctagccacc atggactaca
     2641 aagaccatga cggtgattat aaagatcatg acatcgatta caaggatgac gatgacaagc
     2701 ttggctccat ggcagaaatc ggtactggct ttccattcga cccccattat gtggaagtcc
     2761 tgggcgagcg catgcactac gtcgatgttg gtccgcgcga tggcacccct gtgctgttcc
     2821 tgcacggtaa cccgacctcc tcctacgtgt ggcgcaacat catcccgcat gttgcaccga
     2881 cccatcgctg cattgctcca gacctgatcg gtatgggcaa atccgacaaa ccagacctgg
     2941 gttatttctt cgacgaccac gtccgcttca tggatgcctt catcgaagcc ctgggtctgg
     3001 aagaggtcgt cctggtcatt cacgactggg gctccgctct gggtttccac tgggccaagc
     3061 gcaatccaga gcgcgtcaaa ggtattgcat ttatggagtt catccgccct atcccgacct
     3121 gggacgaatg gccagaattt gcccgcgaga ccttccaggc cttccgcacc accgacgtcg
     3181 gccgcaagct gatcatcgat cagaacgttt ttatcgaggg tacgctgccg atgggtgtcg
     3241 tccgcccgct gactgaagtc gagatggacc attaccgcga gccgttcctg aatcctgttg
     3301 accgcgagcc actgtggcgc ttcccaaacg agctgccaat cgccggtgag ccagcgaaca
     3361 tcgtcgcgct ggtcgaagaa tacatggact ggctgcacca gtcccctgtc ccgaagctgc
     3421 tgttctgggg caccccaggc gttctgatcc caccggccga agccgctcgc ctggccaaaa
     3481 gcctgcctaa ctgcaaggct gtggacatcg gcccgggtct gaatctgctg caagaagaca
     3541 acccggacct gatcggcagc gagatcgcgc gctggctgtc gacgctcgag atttccggcg
     3601 agccaaccac tgaggatctg tactttcaga gcgataactc tagaatgaag cagagttcca
     3661 acgtgccggc tttcctcagc aagctgtgga cgcttgtgga ggaaacccac accaacgagt
     3721 tcatcacctg gagtcagaat ggacaaagtt ttctggtctt ggatgagcaa agatttgcaa
     3781 aggaaattct tcctaagtac ttcaaacaca ataacatggc gagctttgtg agacaactaa
     3841 atatgtatgg cttccgaaaa gtagtgcata tcgaatctgg aattatcaaa caggaaagag
     3901 atggccctgt tgaatttcag catccttatt tcaagcaagg ccaggatgac ctgttggaga
     3961 acattaaaag gaaggtttca tcttcaaaac cagaggaaaa taaaattcgt caggaagatt
     4021 taacaaaaat tattagtagt gctcagaagg ttcaaataaa acaagaaact attgagtcca
     4081 ggctttcaga attaaaaagt gagaatgaat ccctttggaa ggaggtgtca gaactaagag
     4141 caaagcatgc ccagcagcaa caagttattc ggaagattgt ccagtttatt gttacattgg
     4201 ttcagaataa tcaacttgtg agtttaaaac gtaaaaggcc tctacttcta aacacaaatg
     4261 gagccccaaa gaagaatcta tatcagcaca tagtcaaaga accaactgat aatcaccatc
     4321 ataaagttcc acacagcagg actgaaggtt taaagtcaag agaacggatt tcagatgaca
     4381 taattattta tgatgttact gacgataatg tggatgaaga aaatattcca gttattccag
     4441 aaacaaatga ggatgttgta gtggattcct ccaaccagta tcctgacatt gtcattgttg
     4501 aagatgacaa cgaggatgag tatgctcctg tcattcagag tggagagcag agtgaaccag
     4561 ccagagaacc cttacgtgtg gggagtgctg gcagcagcag ccctctcatg tctagtgctg
     4621 tccagctaaa cggctcctcc agtctgacct cagaagaccc tgtgaccatg atggactcca
     4681 ttctgaatga caacattaac ctgttaggaa aggttgagct gttggattac cttgacagta
     4741 ttgattgcag tttagaggac ttccaagcta tgctctcagg aagacagttt agcatagacc
     4801 cagatcttct ggttgattct gagaataagg gactagaagc taccaagagc agtgttgttc
     4861 aacatgtgtc agaagaggga agaaaatcta aatccaagcc agacaaacaa cttatccagt
     4921 atactgcctt tccacttctt gcattcctgg atgggaactc tgcatctgct attgaacagg
     4981 ggagtacaac tgcatcgtca gaagttgtgc cttctgtaga taaacccata gaagtcgatg
     5041 agctcctgga tagcagcctg gatccagaac cgacccagag taagcttgtc cgcctggaac
     5101 cattgactga agcggaagct agtgaagcca cactcttcta tttatgtgaa cttgctcctg
     5161 cacctctgga tagtgatatg ccgcttttag atagttaagc ggccgcgccc ctctccctcc
     5221 ccccccccta acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat
     5281 atgttatttt ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct
     5341 gtcttcttga cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg
     5401 ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta
     5461 gcgacccttt gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag
     5521 ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg
     5581 atagttgtgg aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg gctgaaggat
     5641 gcccagaagg taccccattg tatgggatct gatctggggc ctcggtgcac atgctttaca
     5701 tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga accacgggga cgtggttttc
     5761 ctttgaaaaa cacgataata cctccggaat gattgaacaa gatggattgc acgcaggttc
     5821 tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg
     5881 ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac
     5941 cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc
     6001 cgcgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg
     6061 gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga
     6121 gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg
     6181 cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg
     6241 tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt
     6301 cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc
     6361 ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg
     6421 gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga
     6481 gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc
     6541 gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagtcgaca atcaacctct
     6601 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
     6661 atgtggatac gctgctttaa tgcctttgta tcatgcgtta actaaacttg tttattgcag
     6721 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     6781 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggaatt
     6841 gactcaaatg atgtcaatta gtctatcaga agctcatctg gtctcccttc cgggggacaa
     6901 gacatccctg tttaatattt aaacagcagt gttcccaaac tgggttctta tatcccttgc
     6961 tctggtcaac caggttgcag ggtttcctgt cctcacagga acgaagtccc taaagaaaca
     7021 gtggcagcca ggtttagccc cggaattgac tggattcctt ttttagggcc cattggtatg
     7081 gctttttccc cgtatccccc caggtgtctg caggctcaaa gagcagcgag aagcgttcag
     7141 aggaaagcga tcccgtgcca ccttccccgt gcccgggctg tccccgcacg ctgccggctc
     7201 ggggatgcgg ggggagcgcc ggaccggagc ggagccccgg gcggctcgct gctgccccct
     7261 agcgggggag ggacgtaatt acatccctgg gggctttggg ggggggctgt ccctgatatc
     7321 tataacaaga aaatatatat ataataagtt atcacgtaag tagaacatga aataacaata
     7381 taattatcgt atgagttaaa tcttaaaagt cacgtaaaag ataatcatgc gtcattttga
     7441 ctcacgcggt cgttatagtt caaaatcagt gacacttacc gcattgacaa gcacgcctca
     7501 cgggagctcc aagcggcgac tgagatgtcc taaatgcaca gcgacggatt cgcgctattt
     7561 agaaagagag agcaatattt caagaatgca tgcgtcaatt ttacgcagac tatctttcta
     7621 gggttaatct agctgcatca ggatcatatc gtcgggtctt ttttccggct cagtcatcgc
     7681 ccaagctggc gctatctggg catcggggag gaagaagccc gtgccttttc ccgcgaggtt
     7741 gaagcggcat ggaaagagtt tgccgaggat gactgctgct gcattgacgt tgagcgaaaa
     7801 cgcacgttta ccatgatgat tcgggaaggt gtggccatgc acgcctttaa cggtgaactg
     7861 ttcgttcagg ccacctggga taccagttcg tcgcggcttt tccggacaca gttccggatg
     7921 gtcagcccga agcgcatcag caacccgaac aataccggcg acagccggaa ctgccgtgcc
     7981 ggtgtgcaga ttaatgacag cggtgcggcg ctgggatatt acgtcagcga ggacgggtat
     8041 cctggctgga tgccgcagaa atggacatgg ataccccgtg agttacccgg cgggcgcgct
     8101 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     8161 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     8221 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     8281 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     8341 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     8401 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     8461 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     8521 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     8581 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     8641 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     8701 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     8761 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     8821 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     8881 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     8941 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     9001 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     9061 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     9121 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     9181 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     9241 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     9301 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     9361 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgag
//
