LOCUS       sequence-390792-        8687 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Drives specific expression in the OLQ neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8687)
  AUTHORS   Nava S, Palma W, Wan X, Oh JY, Gharib S, Wang H, Revanna JS, Tan M, 
            Zhang M, Liu J, Chen CH, Lee JS, Perry B, Sternberg PW
  TITLE     A cGAL-UAS bipartite expression toolkit for Caenorhabditis elegans 
            sensory neurons.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Dec 19;120(51):e2221680120. doi: 
            10.1073/pnas.2221680120. Epub 2023 Dec 14.
  PUBMED    38096407
REFERENCE   2  (bases 1 to 8687)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-12-19"; volume: "120(51):e2221680120. 
            doi"; pages: " 10.1073/pnas.2221680120. Epub 2023 Dec 14"
COMMENT     Sequence Label: pWP034
FEATURES             Location/Qualifiers
     source          1..8687
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             5745..5894
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     promoter        6432..6536
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6537..6605,6606..7397)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6755..6774)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7568..8156
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8057..8076
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8310..8327
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8444..8465
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8480..8485,8486..8503,8504..8510)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8518..8534
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8523..8545
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8542..8558
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8542..8558
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgcat gcgcggccgc actgactggg ccggccttgg attaacgtcg catccttctg
       61 caattccacc gtgggccatt tcttcaaaga ccttggctcc accggttgcc ccacccacgg
      121 ttaccgttcc ttctaatatt gctccatcaa ccacaggtca tcaatcacaa caaactcgtc
      181 caacaccaac tactcatcga ccaggaataa ctcctccttt ggctccaaaa acaatttatc
      241 caagttcact ccaaactgga agttcttctc caactccacc tggaactagt tcaataattg
      301 ttgttgctgg ttcaagagct tcatctaatt acccaacaac tgcttccata gaaactgacg
      361 gatcatctga agaacaagaa gaggaaaaca ctagaattct tccagaggag acattatcac
      421 cggcatccgc tcgattatgg actcctgata tgatttccaa gtatatttct tcaaaggcaa
      481 tctctacaga gtctgtttca tctgctgaac aaccttcata tttaagttca aatttaccac
      541 aatcttcctc agttgcttcc gttaaaccgc cttctaactc atcctctgtt ggtcaaaata
      601 ttgaagtgac cgtaccttca tactttccag ttgaacaatc taagtctgtt acacaagcac
      661 ctattccacc tgcatcttca ccatcagcac catctattcc atctatacct tctacactgc
      721 ctgttgctcc atcatcctct tctgttccat cttctccatc tgcaccgagt gaaagcactt
      781 ccacagtttc tgaaacacct gcaccagtaa acgtaaaccc tgcatcaagt aacttctaac
      841 aacatattta ttttgcaatc aaaaccattt cagatgagca tgaagcagaa gaagggccat
      901 acgagccact cagtccacca caaccagcag aaccaaatca tgttgaaggt aatttgtcta
      961 ttcattttgt ttcttcttct atttttcaaa aacactcgca cggtttcgtt cacctttacc
     1021 attttgtttg aacatttttg aacgtttcca gaatatcaga ataccgttga taccataatc
     1081 gagaaatcag gtgagaacat tcatagatcc gacgctgaaa attttttatt aatcaagaaa
     1141 taattagatt caatttggtt ctgaaaatct ttaaaattat atttttgaaa tgaatgtaca
     1201 gaaaagtagt aaattattta cccaaatctg tgaacttttc agcaacacct gcaccagcac
     1261 cagtcacaca gcctgctgtg cagccagcgc cgggacctgt cgagcacaga tacgagattc
     1321 cagctccagg tcctgctcca ggtccagctc ttgagccagc tccggctcca acgtccgctc
     1381 ctcaaatagt tgaaccactt ccacccgttc aaccacttcc tcaaccgcaa cctacggaac
     1441 cggaggagcc acttccgatt gcaacacctg ccccgcaacc tactgagcac acttatggag
     1501 ctcaaggtcc aaatattgtt ccagtagtaa cagcagcacc atatgttcca caggaaactc
     1561 aagccccaat tgctccaagt aatccaggtg cgaattaata cgcataataa gcattgttaa
     1621 atgataattt ttagaaccag ttcaaccaaa tccatcgcaa acagtttcca ttcctgaacc
     1681 aactggaaat attgaagaag cagaacatgt tgatgctaag ccatcatatc caggacaatc
     1741 gtcttataat aatttggaag aagatcatga agagggtcac atcgctgagc ttcctgctct
     1801 tgaaccagtt tcagttccaa cggcagcacc atcccttcct ggagagtcaa attacaggta
     1861 acatagatga catttgatag atctgtatct atgattttca gtaatttgga agaagatcat
     1921 gatgacaaac atcatttagt tgaaggacca tctgtacctt ccactccaac tcaaacatct
     1981 agtggatcaa gttacagtat tttggaagaa gacaaccaag aaacaaataa ttcaggagga
     2041 ccatcttaca ggtatctttt tatttacagt gaaaaagtgg cagtcctatg cttattttga
     2101 acttccattt tagtaaaaaa ttaatcatag tcagaaattt ttttttctaa ttttggcaga
     2161 gatcaaaaaa ttattggagt gttgagttat gatatttggt tactttagaa tataccaaat
     2221 cttagctgac tgactgaatc gtctgactga cggttccttt ggaaaggaaa aatatgtata
     2281 attattttgt aaaattggca ctttctcaaa attgtttttt atataattat tgcatgatta
     2341 ttatttgagc actgttgata tctcggctga aattgatgtt ggaaaagggt aagatgcatg
     2401 atagttagga taataattag atttgaaact gtaattgaat gcatatttga ccttctctcc
     2461 tattttagag gagaacaaca atcatcggag actaaaactg aaacaaagag tgaaactctg
     2521 accagcgggt aatgtttgaa atctagaaac tattgactta tgttaatgat tatgatagaa
     2581 aagataatga atatgaagaa caagattatg agactgagac tcaaccaaca gaagatgagt
     2641 cagaagatca tgtggaatcg attaggtgag aaacttgttg taaaaaagaa acaataagtt
     2701 gggataacaa tgtgacgtca ggaaggaaaa gtgttgtggt gaaaggatga aaatatgttc
     2761 aatatgtttt aaaaataata atttataatt tttgattaac ttaagtattg gtcaattgtt
     2821 ggtggtgtgc tcagattttt cataacacag ttctttattt ttcagcagtt ttgataaaga
     2881 gcaagtccct caagttgttg ttccaacaaa acctgccggt ggatacaggt aagaaattga
     2941 actgaaagct tcaaaatacc acaattaaga tctcaaaaag tttaaattct attgtgtcaa
     3001 tattttcaga gatggtggaa tggtggagaa cactgcacca caaccaacaa cataccagcg
     3061 tccacatcca ataaaagttc aagtcagacc agctactaaa ccatatcggc cacgtccatc
     3121 acctgcacca aggattcagc caagaccata taatcctcaa ccagtcgtag ttcaaaggcc
     3181 tcagtttaga cctcaacctc aaccaagacc tcaaccccag ccacaaccac agccacaacc
     3241 acagccacaa ccacaacaac cttatattca acgaccagct gtaagacaat tataattttt
     3301 cgaatattca tctagtctag aaaaaagaga cacatgttct taaatctgaa actttcagct
     3361 cacacttccc ttccaacaac ctcagtatcc tccaccaatt ccattccaac caagacctgc
     3421 tccatttatt ccattccaac caatggttca gagaccatct ccagctggat gctgtggtgg
     3481 acagcttttc agtcaacaag gagggttatg tatgccaatc agtttcaatc catgccaaca
     3541 acaacaacag caaaacaact gcggtggtgg ttgtggcggt ggatgttctg gaggtggaaa
     3601 ctcttgtggt ggaggatgca attctggagg aagttgtggt ggaggaggag gtggatgtag
     3661 ttcttcatgt tgccaaccac aaacatccgc gtgctgcaat cagatggtat ctacttgctg
     3721 tcaaccacaa caaatggctt gttgtaacca gcaggtattc tattttagtc tattagtcgc
     3781 ttaattaata tcgtaataat ttcagaacca gtcatgctgc ccacaacaag ctcaacaatc
     3841 gtgttgctgt cccactcaac aacccatttg ccggcataaa cggtaactgc cgtccatctt
     3901 tgaaatgttt caaattttta atttccagat cacttggcta cgcaatggga atctgtcagc
     3961 gaaggagagc ctgagttttt actaaatagg aagtcattaa taatctcatt catcacaaag
     4021 tcctctgtta ttttcctcga ggaatgttat tttattgtgt ctcaaattta tttattctct
     4081 aaattatacc taactctgaa cgagtcgaac cgtcaattgt tcagggtcca ccggttctca
     4141 actttttgct tctctcaatt catttttttt atcattttaa ttaaatttcg gtcagtttgt
     4201 gaaagtgtat tcttttaatt aagttttttt tattaattat agacatttct tatataaaac
     4261 atttatttaa atgagagtct tggtcgaaaa tatataatac agaaatggaa ataaaaaata
     4321 tgtttaaagg atcagcatca gcatcacgga tgacattccc aaggaacatc ttcaagactc
     4381 cacgggtttc cacgtagatc aatccagaga ttctcttgac tcctcctctt cttgcgagac
     4441 gacggatggc tggcttggtg atacctttga tgttatcacg gagatggcat atgattttgt
     4501 tttttagagt tttaatagca atgctcgtgt gaagatgatt tttaaagagc cagtttcagg
     4561 cttcatgtca ttatgctatt tgccgatttt ctttcaggaa ggtttacggt ttgaataatg
     4621 gcacgaagat acatagatgt atttataatt taccgatggc ttgattgcat tgtaataaac
     4681 cgtcaggaaa gaaaaataac attttcccag accctatatg ctacatatta ctcagtgaac
     4741 catagaaatg tcaaatttcc ggttatgagg cataagtgag aataaaggta tgtgagcacc
     4801 gtagtttctt atttacaatt tcccatcgaa gaaaaagtaa aatattctct gaatctaact
     4861 tgtattaatg ggtaatgcat ctagtgctgt tactgctggt attaaaaatc aagttaatgc
     4921 ttcgaacaat gctattaata aattggctga tatgagtggg cgtggatttt tagcggatct
     4981 ggcgatggat gctgttagaa ctggaaacca tgaagaattg gataaacaaa tattagaggt
     5041 aattcaagtg tttccgaata taccaattta gtaatttaga aagttcaacc aatgctctat
     5101 aacaacggcc agggcgcgcc tctagaggat ccccgggatt ggccaaagga cccaaaggta
     5161 tgtttcgaat gatactaaca taacatagaa cattttcagg aggacccttg gctaggatgg
     5221 gccctaaaaa gaagcgtaaa gctagcatga agctgttgtc ttcaatggaa caagcttgcg
     5281 acatatgccg gttaaaaaaa ctcaggtgct cgaaggaaaa gcccaagtgt gccaagtgtc
     5341 tgaagaacaa ctcggagtgc cgttattctc ccaaggctat gaggtcccca ctgaccaggg
     5401 cgcatctgac agaggtagaa tcaaggttgg aaaaattgga agatttgttt ctgctcatgt
     5461 ttcctcggga aaacctggac agcattttga acatggactc tttggacgaa gtgaaagtaa
     5521 tgttgaaaca attatatatg caggactatg gtgacacgga tgctgctatg gatagcttga
     5581 cccctatgga aaatggcatg tctgaagggc cgagaaaaca catgaagact gcgacatcat
     5641 cgccaaaagt ggatgaagat ggaagccaaa gtcagttgag tgtatcgccg gaattcccgc
     5701 tgcagtctgg tggcggaggg gctcctaggg gttccggacg ggctgacgca ttggacgatt
     5761 ttgatctgga tatgctggga agtgacgccc tcgatgattt tgaccttgac atgcttggtt
     5821 cggatgccct tgatgacttt gacctcgaca tgctcggcag tgacgccctt gatgatttcg
     5881 acctggacat gctgattaac taaggtaccg aattcgctag ccggccatac aagtaatccg
     5941 gatgatcgac gccaacgtcg ttgaattttc aaattttaaa tactgaatat ttgttttttt
     6001 tcctattatt tatttattct ctttgtgttt tttttcttgc tttctaaaaa attaattcaa
     6061 tccaaatcta aacatttttt tttctctttc cgtctcccaa ttcgtattcc gctcctctca
     6121 tctgaacaca atgtgcaagt ttatttatct tctcgctttc atttcattag gacgtggggg
     6181 gaattggtgg aagggggaaa cacacaaaag gatgatggaa atgaaataag gacacacaat
     6241 atgcaacaac attcaattca gaaatatgga ggaaggttta aaagaaaaca taaaaatata
     6301 tagaggagga aggaaaacta gtaaaaaata agcaaagaaa ttaggcgaac gatgagaatt
     6361 gtcctcgctt gggcccaaaa ggcctttttt ttttggcgcc gacgtcaggt ggcacttttc
     6421 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     6481 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     6541 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     6601 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     6661 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     6721 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     6781 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     6841 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     6901 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     6961 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     7021 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     7081 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     7141 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     7201 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     7261 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     7321 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     7381 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     7441 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     7501 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     7561 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     7621 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     7681 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     7741 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     7801 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     7861 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     7921 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     7981 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     8041 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     8101 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     8161 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     8221 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     8281 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     8341 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     8401 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
     8461 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
     8521 tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagctgtaag
     8581 tttaaacatg atcttactaa ctaactattc tcatttaaat tttcagagct taaaaatggc
     8641 tgaaatcact cacaacgatg gatacgctaa caacttggaa atgaaat
//
