LOCUS       sequence-390793-        3884 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Co-expression plasmid for the OLL neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3884)
  AUTHORS   Nava S, Palma W, Wan X, Oh JY, Gharib S, Wang H, Revanna JS, Tan M, 
            Zhang M, Liu J, Chen CH, Lee JS, Perry B, Sternberg PW
  TITLE     A cGAL-UAS bipartite expression toolkit for Caenorhabditis elegans 
            sensory neurons.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Dec 19;120(51):e2221680120. doi: 
            10.1073/pnas.2221680120. Epub 2023 Dec 14.
  PUBMED    38096407
REFERENCE   2  (bases 1 to 3884)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-12-19"; volume: "120(51):e2221680120. 
            doi"; pages: " 10.1073/pnas.2221680120. Epub 2023 Dec 14"
COMMENT     Sequence Label: pWP171
FEATURES             Location/Qualifiers
     source          1..3884
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1629..1733
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1734..1802,1803..2594)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1952..1971)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2765..3353
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3254..3273
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3507..3524
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3641..3662
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3677..3682,3683..3700,3701..3707)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3715..3731
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3720..3742
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3739..3755
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3739..3755
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgcat gcgcggccgc actgactggg ccggcccaaa caaatggttg gagagccact
       61 cgccaagtga gtttcttttg agattttttg tccaccattt ccggagccca acacaacaaa
      121 aaggcacacg gccgtgaaat tggtggggct gaatgagcgc acatacctct caagcattaa
      181 catttttttc gattccatat ctcaactaat ttcagaggcg cgcctctaga ggatccccgg
      241 gattggccaa aggacccaaa ggtatgtttc gaatgatact aacataacat agaacatttt
      301 caggaggacc cttggctagg atgggcccta aaaagaagcg taaagctagc atgaagctgt
      361 tgtcttcaat ggaacaagct tgcgacatat gccggttaaa aaaactcagg tgctcgaagg
      421 aaaagcccaa gtgtgccaag tgtctgaaga acaactcgga gtgccgttat tctcccaagg
      481 ctatgaggtc cccactgacc agggcgcatc tgacagaggt agaatcaagg ttggaaaaat
      541 tggaagattt gtttctgctc atgtttcctc gggaaaacct ggacagcatt ttgaacatgg
      601 actctttgga cgaagtgaaa gtaatgttga aacaattata tatgcaggac tatggtgaca
      661 cggatgctgc tatggatagc ttgaccccta tggaaaatgg catgtctgaa gggccgagaa
      721 aacacatgaa gactgcgaca tcatcgccaa aagtggatga agatggaagc caaagtcagt
      781 tgagtgtatc gccggaattc ccgctgcagt ctggtggcgg aggggctcct aggtgcctcg
      841 acctcaagac ccaagtccaa accccacaag gaatgaagga gatctccaac atccaagtcg
      901 gagacctcgt cctctccaac accggataca acgaggtcct caacgtcttc ccaaagtcca
      961 agaagaagtc ctacaagatc accctcgagg acggaaagga gatcatctgc tccgaggagc
     1021 acctcttccc aacccaaacc ggagagatga acatctccgg aggactcaag gagggaatgt
     1081 gcctctacgt caaggagtaa ggtaccgaat tcgctagccg gccatacaag taatccggat
     1141 gatcgacgcc aacgtcgttg aattttcaaa ttttaaatac tgaatatttg ttttttttcc
     1201 tattatttat ttattctctt tgtgtttttt ttcttgcttt ctaaaaaatt aattcaatcc
     1261 aaatctaaac attttttttt ctctttccgt ctcccaattc gtattccgct cctctcatct
     1321 gaacacaatg tgcaagttta tttatcttct cgctttcatt tcattaggac gtggggggaa
     1381 ttggtggaag ggggaaacac acaaaaggat gatggaaatg aaataaggac acacaatatg
     1441 caacaacatt caattcagaa atatggagga aggtttaaaa gaaaacataa aaatatatag
     1501 aggaggaagg aaaactagta aaaaataagc aaagaaatta ggcgaacgat gagaattgtc
     1561 ctcgcttggg cccaaaaggc cttttttttt tggcgccgac gtcaggtggc acttttcggg
     1621 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     1681 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     1741 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     1801 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     1861 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     1921 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     1981 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     2041 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     2101 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     2161 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     2221 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     2281 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     2341 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     2401 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     2461 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     2521 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     2581 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     2641 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     2701 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     2761 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     2821 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     2881 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     2941 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     3001 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     3061 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     3121 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     3181 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     3241 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     3301 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     3361 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     3421 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     3481 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     3541 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     3601 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
     3661 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
     3721 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag ctgtaagttt
     3781 aaacatgatc ttactaacta actattctca tttaaatttt cagagcttaa aaatggctga
     3841 aatcactcac aacgatggat acgctaacaa cttggaaatg aaat
//
