LOCUS       sequence-390794-        2237 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  In vitro expression of Der p 2, a major common allergen from 
            Dermatophagoides pteronyssinus (dust mite), with synthetic site for 
            glycosylation encoded (GGNWTT).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2237)
  AUTHORS   Thames AH, Moons SJ, Wong DA, Boltje TJ, Bochner BS, Jewett MC
  TITLE     GlycoCAP: A Cell-Free, Bacterial Glycosylation Platform for Building
            Clickable Azido-Sialoglycoproteins.
  JOURNAL   ACS Synth Biol. 2023 Apr 11. doi: 10.1021/acssynbio.3c00017.
  PUBMED    37040463
REFERENCE   2  (bases 1 to 2237)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2023 Apr 11. doi: 10.1021/acssynbio.3c00017."
COMMENT     Sequence Label: Derp2.pJL1
FEATURES             Location/Qualifiers
     source          1..2237
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             129..151
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(182..201)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(183..201)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(229..817)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(309..328)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(928..947)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             939..1754
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1012..1031)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      complement(1772..1819)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     1815..1833
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(1888..1905)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gaaacggttt accacgatga ataatacacg gttcgctacc atgacaaccc ggaaccagaa
       61 cttttttgat ttcgtggttg gcgcaatctt taacatccac ctgatcgatt tttttctcca
      121 tatgtatatc tccttcttaa agttaaacaa aattatttct agagggaaac cgttgtggtc
      181 tccctatagt gagtcgtatt aatttcgcgg gatcgcgttg ctggcgtttt tccataggct
      241 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
      301 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
      361 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
      421 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
      481 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
      541 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
      601 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
      661 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
      721 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
      781 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tctcacgttg
      841 tgtctcaaaa tctctgatgt tacattgcac aagataaaaa tatatcatca tgaacaataa
      901 aactgtctgc ttacataaac agtaatacaa ggggtgttat gagccatatt caacgggaaa
      961 cgtcttgctc gaagccgcga ttaaattcca acatggatgc tgatttatat gggtataaat
     1021 gggctcgcga taatgtcggg caatcaggtg cgacaatcta tcgattgtat gggaagcccg
     1081 atgcgccaga gttgtttctg aaacatggca aaggtagcgt tgccaatgat gttacagatg
     1141 agatggtcag actaaactgg ctgacggaat ttatgcctct tccgaccatc aagcatttta
     1201 tccgtactcc tgatgatgca tggttactca ccactgcgat ccccgggaaa acagcattcc
     1261 aggtattaga agaatatcct gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc
     1321 tgcgccggtt gcattcgatt cctgtttgta attgtccttt taacagcgat cgcgtatttc
     1381 gtctcgctca ggcgcaatca cgaatgaata acggtttggt tgatgcgagt gattttgatg
     1441 acgagcgtaa tggctggcct gttgaacaag tctggaaaga aatgcataag cttttgccat
     1501 tctcaccgga ttcagtcgtc actcatggtg atttctcact tgataacctt atttttgacg
     1561 aggggaaatt aataggttgt attgatgttg gacgagtcgg aatcgcagac cgataccagg
     1621 atcttgccat cctatggaac tgcctcggtg agttttctcc ttcattacag aaacggcttt
     1681 ttcaaaaata tggtattgat aatcctgata tgaataaatt gcagtttcat ttgatgctcg
     1741 atgagttttt ctaatcagaa ttggctttca gcaaaaaacc cctcaagacc cgtttagagg
     1801 ccccaagggg ttatgctagt tattgctcag cggtggcagc agccaactca gcttcctttc
     1861 gggctttgtt agcagccggt cgacttaatg gtgatgatgg tgatgaccac cggcacgggt
     1921 tgtccaatta ccacctgtgg ttgcacgacc ggatccatca cgaatttttg catgggttgc
     1981 aatggcacat gccagaacac catcatcacc catcacttta acggtcacaa caacattttc
     2041 gcttttcggt gcaattttcg gcacgttcca ggtatatttg atatcatact gctgaccttt
     2101 aaccagcgga catttcatat agtggcatgc attcggatca atacccggaa catcaacttc
     2161 cagaccatca atgctggctt tgatttcaat cttggcggtt ttggtattct gatttgcttc
     2221 aaaaactgct tccagct
//
