LOCUS       sequence-390795-        6780 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Drives specific expression in the URX neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6780)
  AUTHORS   Nava S, Palma W, Wan X, Oh JY, Gharib S, Wang H, Revanna JS, Tan M, 
            Zhang M, Liu J, Chen CH, Lee JS, Perry B, Sternberg PW
  TITLE     A cGAL-UAS bipartite expression toolkit for Caenorhabditis elegans 
            sensory neurons.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Dec 19;120(51):e2221680120. doi: 
            10.1073/pnas.2221680120. Epub 2023 Dec 14.
  PUBMED    38096407
REFERENCE   2  (bases 1 to 6780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-12-19"; volume: "120(51):e2221680120. 
            doi"; pages: " 10.1073/pnas.2221680120. Epub 2023 Dec 14"
COMMENT     Sequence Label: pWP003
FEATURES             Location/Qualifiers
     source          1..6780
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             3838..3987
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     promoter        4525..4629
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4630..4698,4699..5490)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4848..4867)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5661..6249
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6150..6169
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6403..6420
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6537..6558
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6573..6578,6579..6596,6597..6603)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6611..6627
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6616..6638
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6635..6651
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6635..6651
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgcat gcgcggccgc actgactggg ccggcccgtg atggagttcg cagcaatgag
       61 tgctcaaatg gagtctgcat gaaaatgatt ttccaggaaa aaagcggtga gatagatcaa
      121 aattaattgt ttattttatt ttctttttac ttattttagg aatcaatcat gtttggagaa
      181 gttgcatacc aaatgcaaaa gaacaaatcc gatcagactg tacaaaaatt actagtggag
      241 aaggtatgta atattcaaat tgttccatat aataaaaaaa aaatatatat ataactttca
      301 ggaaaccttg aagtttgcac atgcgatggt aatttatgca acaatgacgt caatttaaat
      361 ctaatactaa taattatgtt ttctgcagct gtgttattat tatgatgagt tgttcattaa
      421 acctacatat cggaatcaaa tcaaacttag atctctattc ttttcatttt ccatcggttt
      481 tatatagaac ttttgccgaa gtattttgat tgcataccaa ataaatgttt ttacgttgtt
      541 ggaaatgttt attaaaattt aaaggaacac agaatagcca aagtgttttt aatttccaaa
      601 ttttttttta ctcctcgaac agtacccttg aacttcagct cgctattttg aatattcctg
      661 tcacgaagtc ggtgaataca aagattcact cgctcacgac ttattaataa atctaaggat
      721 ttattaacaa atgattaaat gtaaagattg attcattgtt attacgtaac acttgctctt
      781 caacactcaa catcatctct tcaatcaacg gcaacaacca tgcagaagga cggcgtcaga
      841 aaccccgatt caaactaccg ccaaaaggac gattcaaggc gcatgaggtc tcgccacgac
      901 acatacaaca acgactgcga ccgtactctt gtcatcatgg cgcagcgcac attggcctat
      961 atcgtcagtc ttcatcggtc ttcaccaccg cagcacgtgc ccgacggtgg cacgtttaaa
     1021 tgaatgttga gtgttagtga ttcaaggttt tccccttgaa tcactcgtaa taaatgagtt
     1081 aattaaataa gagtcttttg ttagttccta tcagtacctc atgggccgga aaccactccg
     1141 aaacaattcc cataatttta ttgttttcca ttttccagga aaaaaaacag ttctttgaca
     1201 ttttcaagcc aaaaactatg tgtgttccgt cttaagacca tgcattggaa attacgttcg
     1261 tcactatttt tgtgtgtatg tgttttggat tcctcttctc acatttgcgg tcacaacagt
     1321 atcgttcatc ggtttttatg ttgtgataaa atagagaaaa agtaaaacga gcatattttt
     1381 gatagcggaa gaaaaatttt gagattacat ataacaaagc ggaaagtgat tatcaaatgt
     1441 caaattatca aataatttgg aagcttttca attcaatata cagggttgat tttcaatagg
     1501 tggtgcaagt aaaaaagaaa tactgtattt atatttaaag ataaatacat tttgtttaaa
     1561 cacgtaaata ttgtttcaag ctggccgcat atcaaaataa aaccgtacaa aaactttcca
     1621 gtttttctta attttcagtc aaggttttag taaaatagcg aggcgacgaa aatatataac
     1681 tttccgagaa gttcagagca gttaataatt attatcaact ttttacagaa tggtttacat
     1741 tttttcaaca ttaattatag ttaaaggaca atacattttt caaaatattc actgtaaata
     1801 tgaatgaata cttttggaaa ataatcaaaa agctgactga ctactgttaa acaatggcgg
     1861 agtgataaac tagcaaattc acttattatg cacataatta taatgcagga taagatgact
     1921 gactttcaag acattcacta tttaatgatt agacttccat ttttgaccat cccattttga
     1981 cataagaact ccttgatatt ttgttctctt tgaaaagtct tgttttactc tactttaaat
     2041 gaattcattt tcgtcatgga cgatctcttc aaaaagtatg tgggcacctt tactttgaat
     2101 acacgcaatc ttcaccgtag tttcatgttg ctcgaatgaa tattcaatga ttggagggca
     2161 agatttatga agagattgcc gtgaaatcat tttgcgaggc gaatcatcga ggaagcaact
     2221 gattcgtgat gaataatttg cgcggtggat aagtttttgg tttcaaagta cccccccccc
     2281 ccccgagaag aggtggaaaa aagttgaaga attagatgaa ggagaagtcc aagtgttacg
     2341 tcaacaattt acaaatgcat agagttggta taattagttt cggagggtgt cggtggctaa
     2401 atttatctgt tagttacctc ccagcatgaa ttccgtgtat tcactgtgtt cagtccctcg
     2461 actgctcggt tatacagctg atagatagac gaggaagaag aaaacacaca aaaacgtaaa
     2521 gtgggcctct ttaaaaaaat ttaaaaaaca aaataaggac acaaaaatat aaatatgcat
     2581 tgttttcatt tttcaaaacg agggtagctt cgagaaattt ccattttcaa atcaaacgaa
     2641 ttgctacaac aatgtcaaaa tgttaaaatt tttcttggtg gtactgtatg actctaaagt
     2701 ttgatctgga aaaatttgtt ttttcgtaga taacgtttct tacctaaaaa gcggttttta
     2761 acctgatagt tgagataacc tggtttatac aataaatttt caaatgagat aatttttaat
     2821 aactttaaaa aaaaattttt ttatatcgtt ggttttcgaa acttgatttt cagcaaattt
     2881 ttaaaatttg aatcaacttg ctctcaaaga cacgtttatt gtcttacgac ttttaaaacc
     2941 ggagtaaatt taatttttcg atgaatcaat ttcagataaa tctcagaaca agcactttac
     3001 tgtttcttac ttgttttatg caatttacca tttcaagttt tctatgcccc cgcgttcttt
     3061 taccaaacgc cttccagtgg acactctaga atttcttcag cacaagaagg cgtggcctct
     3121 gaaaaggcgg agcaaagtgc gcggagccca agttagtgga gaagacggac cagtatataa
     3181 gccacgagca gtgctcagtc cacacggcgc gcctctagag gatccccggg attggccaaa
     3241 ggacccaaag gtatgtttcg aatgatacta acataacata gaacattttc aggaggaccc
     3301 ttggctagga tgggccctaa aaagaagcgt aaagctagca tgaagctgtt gtcttcaatg
     3361 gaacaagctt gcgacatatg ccggttaaaa aaactcaggt gctcgaagga aaagcccaag
     3421 tgtgccaagt gtctgaagaa caactcggag tgccgttatt ctcccaaggc tatgaggtcc
     3481 ccactgacca gggcgcatct gacagaggta gaatcaaggt tggaaaaatt ggaagatttg
     3541 tttctgctca tgtttcctcg ggaaaacctg gacagcattt tgaacatgga ctctttggac
     3601 gaagtgaaag taatgttgaa acaattatat atgcaggact atggtgacac ggatgctgct
     3661 atggatagct tgacccctat ggaaaatggc atgtctgaag ggccgagaaa acacatgaag
     3721 actgcgacat catcgccaaa agtggatgaa gatggaagcc aaagtcagtt gagtgtatcg
     3781 ccggaattcc cgctgcagtc tggtggcgga ggggctccta ggggttccgg acgggctgac
     3841 gcattggacg attttgatct ggatatgctg ggaagtgacg ccctcgatga ttttgacctt
     3901 gacatgcttg gttcggatgc ccttgatgac tttgacctcg acatgctcgg cagtgacgcc
     3961 cttgatgatt tcgacctgga catgctgatt aactaaggta ccgaattcgc tagccggcca
     4021 tacaagtaat ccggatgatc gacgccaacg tcgttgaatt ttcaaatttt aaatactgaa
     4081 tatttgtttt ttttcctatt atttatttat tctctttgtg ttttttttct tgctttctaa
     4141 aaaattaatt caatccaaat ctaaacattt ttttttctct ttccgtctcc caattcgtat
     4201 tccgctcctc tcatctgaac acaatgtgca agtttattta tcttctcgct ttcatttcat
     4261 taggacgtgg ggggaattgg tggaaggggg aaacacacaa aaggatgatg gaaatgaaat
     4321 aaggacacac aatatgcaac aacattcaat tcagaaatat ggaggaaggt ttaaaagaaa
     4381 acataaaaat atatagagga ggaaggaaaa ctagtaaaaa ataagcaaag aaattaggcg
     4441 aacgatgaga attgtcctcg cttgggccca aaaggccttt tttttttggc gccgacgtca
     4501 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     4561 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     4621 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     4681 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     4741 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     4801 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     4861 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     4921 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     4981 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     5041 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     5101 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     5161 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     5221 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     5281 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     5341 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     5401 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     5461 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     5521 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     5581 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     5641 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     5701 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     5761 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     5821 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     5881 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     5941 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     6001 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     6061 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     6121 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     6181 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     6241 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     6301 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     6361 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     6421 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     6481 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
     6541 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg
     6601 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac
     6661 gccaagctgt aagtttaaac atgatcttac taactaacta ttctcattta aattttcaga
     6721 gcttaaaaat ggctgaaatc actcacaacg atggatacgc taacaacttg gaaatgaaat
//
