LOCUS       sequence-39086-e         790 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Vick JE, Johnson ET, Choudhary S, Bloch SE, Lopez-Gallego F, 
            Srivastava P, Tikh IB, Wawrzyn GT, Schmidt-Dannert C
  TITLE     Optimized compatible set of BioBrick vectors for metabolic pathway 
            engineering.
  JOURNAL   Appl Microbiol Biotechnol. 2011 Dec;92(6):1275-86. Epub 2011 Oct 28.
  PUBMED    22033566
REFERENCE   2  (bases 1 to 790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Microbiol Biotechnol."; date: "2011-12"; volume: "92(6)"; pages: 
            "1275-86. Epub 2011 Oct 28"
COMMENT     Sequence Label: pACBB-TetT-LVA
FEATURES             Location/Qualifiers
     source          1..790
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(14..31)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(52..84)
                     /codon_start=1
                     /product="C-terminal peptide that mediates degradation in 
                     bacteria through the ClpXP and ClpAP proteases (McGinness 
                     et al., 2006)"
                     /label=ssrA tag (LVA)
                     /note="mutant LVA variant that confers accelerated 
                     degradation under some conditions (Andersen et al., 1998)"
                     /translation="AANDENYALVA"
     CDS             complement(91..714)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MMSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGT
                     RPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPT
                     TDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     619..638
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     promoter        complement(join(752..758,759..776,777..782))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 gcgtaggaga tcagtgatgg tgatggtgat gctcgaggcg gccgcttatt aagctactaa
       61 agcgtagttt tcgtcgtttg cagcaggcct agacccactt tcacatttaa gttgtttttc
      121 taatccgcat atgatcaatt caaggccgaa taagaaggct ggctctgcac cttggtgatc
      181 aaataattcg atagcttgtc gtaataatgg cggcatacta tcagtagtag gtgtttccct
      241 ttcttcttta gcgacttgat gctcttgatc ttccaatacg caacctaaag taaaatgccc
      301 cacagcgctg agtgcatata atgcattctc tagtgaaaaa ccttgttggc ataaaaaggc
      361 taattgattt tcgagagttt catactgttt ttctgtaggc cgtgtaccta aatgtacttt
      421 tgctccatcg cgatgactta gtaaagcaca tctaaaactt ttagcgttat tacgtaaaaa
      481 atcttgccag ctttcccctt ctaaagggca aaagtgagta tggtgcctat ctaacatctc
      541 aatggctaag gcgtcgagca aagcccgctt attttttaca tgccaataca atgtaggctg
      601 ctctacacct agcttctggg cgagtttacg ggttgttaaa ccttcgattc cgacctcatt
      661 aagcagctct aatgcgctgt taatcacttt acttttatct aaacgagaca tcatagatct
      721 cctccttcta ctagacgctc acaattccac acaacatacg agccggaagc ataaagtgta
      781 aagcctgggg
//
