LOCUS       sequence-39089-e         833 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 833)
  TITLE     Jacks Lab Unpublished Plasmids
REFERENCE   2  (bases 1 to 833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pRosa26 LSL luciferase targeting
FEATURES             Location/Qualifiers
     source          1..833
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      28..37
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      135..144
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    169..290
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(180..199)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     234..253
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(715..735)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ggcctttctt tatgtttttg gcgtcttcca tggtggcttt accaacagta ccggaatgcc
       61 aagcttactt agatcgcaga tccacaactt cgtatagaaa ggtatatacg aagcttactg
      121 cagacttact caagccatgg tggctagctt gcggaattcc agacatgata agatacattg
      181 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt
      241 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca
      301 attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaagcaagt
      361 aaaacctcta caaatgtggt atggctgatt atgatctcta gtcaaggcac tatacatcaa
      421 atattcctta ttaacccctt tacaaattaa aaagctaaag gtacacaatt tttgagcata
      481 gttattaata gcagacactc tatgcctgtg tggagtaaga aaaaacagta tgttatgatt
      541 ataactgtta tgcctactta taaaggttac agaatatttt tccataattt tcttgtatag
      601 cagtgcagct ttttcctttg tggtgtaaat agcaaagcaa gcaagagttc tattactaaa
      661 cacagcatga ctcaaaaaac ttagcaattc tgaaggaaag tccttggggt cttctacctt
      721 tctcttcttt tttggaggag tagaatgttg agagtcagca gtagcctcat catcactaga
      781 tggcatttct tctgagcaaa acaggttttc ctcattaaag gcattccacc act
//
