LOCUS       sequence-390938-        6231 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression vector of mouse PPP1CC2. HA tag at N-terminus..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6231)
  AUTHORS   Shimada K, Park S, Oura S, Noda T, Morohoshi A, Matzuk MM, Ikawa M
  TITLE     TSKS localizes to nuage in spermatids and regulates cytoplasmic 
            elimination during spermiation.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Mar 14;120(11):e2221762120. doi: 
            10.1073/pnas.2221762120. Epub 2023 Mar 7.
  PUBMED    36881620
REFERENCE   2  (bases 1 to 6231)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-03-14"; volume: "120(11):e2221762120. 
            doi"; pages: " 10.1073/pnas.2221762120. Epub 2023 Mar 7"
COMMENT     Sequence Label: pCAG1.1-HA/Ppp1cc2
FEATURES             Location/Qualifiers
     source          1..6231
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        17..396
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        398..673
                     /label=chicken beta-actin promoter
     intron          674..1688
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1614..1636
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1696..1715
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             1758..1784
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(2911..2930)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2976..3031
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3030..3049)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(3386..3405)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3387..3405)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3415..3432)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3415..3431)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3424..3446)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3573..4028
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3660..3679)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3870..3891
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4054..4158
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4159..4227,4228..5019)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4377..4396)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5190..5778
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5679..5698
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5932..5949
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6066..6087
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6102..6107,6108..6125,6126..6132)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6140..6156
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6145..6167
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6164..6180
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6164..6180
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6199..6219
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6201..6219
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 gagctcatcg atactcgaca ttgattattg actagttatt aatagtaatc aattacgggg
       61 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg
      121 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
      181 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
      241 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
      301 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
      361 cagtacatct acgtattagt catcgctatt accatggtcg aggtgagccc cacgttctgc
      421 ttcactctcc ccatctcccc cccctcccca cccccaattt tgtatttatt tattttttaa
      481 ttattttgtg cagcgatggg ggcggggggg gggggggggc gcgccaggcg gggcggggcg
      541 gggcgagggg cggggcgggg cgaggcggag aggtgcggcg gcagccaatc agagcggcgc
      601 gctccgaaag tttcctttta tggcgaggcg gcggcggcgg cggccctata aaaagcgaag
      661 cgcgcggcgg gcgggagtcg ctgcgcgctg ccttcgcccc gtgccccgct ccgccgccgc
      721 ctcgcgccgc ccgccccggc tctgactgac cgcgttactc ccacaggtga gcgggcggga
      781 cggcccttct cctccgggct gtaattagcg cttggtttaa tgacggcttg tttcttttct
      841 gtggctgcgt gaaagccttg aggggctccg ggagggccct ttgtgcgggg ggagcggctc
      901 ggggggtgcg tgcgtgtgtg tgtgcgtggg gagcgccgcg tgcggctccg cgctgcccgg
      961 cggctgtgag cgctgcgggc gcggcgcggg gctttgtgcg ctccgcagtg tgcgcgaggg
     1021 gagcgcggcc gggggcggtg ccccgcggtg cggggggggc tgcgagggga acaaaggctg
     1081 cgtgcggggt gtgtgcgtgg gggggtgagc agggggtgtg ggcgcgtcgg tcgggctgca
     1141 accccccctg cacccccctc cccgagttgc tgagcacggc ccggcttcgg gtgcggggct
     1201 ccgtacgggg cgtggcgcgg ggctcgccgt gccgggcggg gggtggcggc aggtgggggt
     1261 gccgggcggg gcggggccgc ctcgggccgg ggagggctcg ggggaggggc gcggcggccc
     1321 ccggagcgcc ggcggctgtc gaggcgcggc gagccgcagc cattgccttt tatggtaatc
     1381 gtgcgagagg gcgcagggac ttcctttgtc ccaaatctgt gcggagccga aatctgggag
     1441 gcgccgccgc accccctcta gcgggcgcgg ggcgaagcgg tgcggcgccg gcaggaagga
     1501 aatgggcggg gagggccttc gtgcgtcgcc gcgccgccgt ccccttctcc ctctccagcc
     1561 tcggggctgt ccgcgggggg acggctgcct tcggggggga cggggcaggg cggggttcgg
     1621 cttctggcgt gtgaccggcg gctctagcct ctgctaacca tgttcatgcc ttcttctttt
     1681 tcctacagct cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaatcta
     1741 gaggatccgc cgccatgtac ccatacgatg ttccagatta cgctgggggc ggggaattca
     1801 tggcggatat cgacaaactc aacatcgaca gcatcatcca acggctgctg gaagtgagag
     1861 ggtccaagcc aggcaagaat gtccagctcc aggagaacga gatccgagga ctctgcctga
     1921 agtctcggga gatcttcctc agtcagccta tccttttaga acttgaagca ccactcaaga
     1981 tatgtggtga catccacggg cagtactatg atttgctccg tctgtttgaa tacggtggct
     2041 ttcctccaga gagcaactat ttgtttctcg gggactatgt ggacaggggc aagcagtccc
     2101 tggagacaat ctgcctcttg ctggcctaca aaatcaagta tccggagaac ttctttcttc
     2161 tcagagggaa ccacgagtgc gccagcatca ataggatcta cggattttat gatgagtgta
     2221 aaagaagata caacattaag ctgtggaaaa cgttcacaga ctgttttaac tgcttgccga
     2281 tagcagccat cgtggacgag aagatattct gctgtcatgg aggtttatca ccagatcttc
     2341 aatctatgga gcagattcgg cgaattatga gaccaactga tgtaccagat caaggtcttc
     2401 tttgtgatct tttgtggtct gaccccgata aagatgtctt aggctggggt gaaaatgaca
     2461 gaggagtgtc cttcacattt ggtgcagaag tggttgcaaa atttctccat aagcatgatt
     2521 tggatcttat atgtagagcc catcaggtgg ttgaagatgg ctatgagttt tttgcaaaga
     2581 ggcagttagt cactctgttt tctgcaccca actactgtgg cgagtttgac aatgcaggcg
     2641 ccatgatgag tgtggatgag accctcatgt gttccttcca gattttaaag cctgcagaga
     2701 aaaagaagcc caacgccacg agacctgtca caccaccacg ggttggatca ggcctgaacc
     2761 cgtccattca gaaagcttca aattatagaa acaacactgt cctatacgag tgagtcgacg
     2821 cggccgcctc gagactcctc aggtgcaggc tgcctatcag aaggtggtgg ctggtgtggc
     2881 caatgccctg gctcacaaat accactgaga tctttttccc tctgccaaaa attatgggga
     2941 catcatgaag ccccttgagc atctgacttc tggctaataa aggaaattta ttttcattgc
     3001 aatagtgtgt tggaattttt tgtgtctctc actcggaagg acatatggga gggcaaatca
     3061 tttaaaacat cagaatgagt atttggttta gagtttggca acatatgccc atatgctggc
     3121 tgccatgaac aaaggttggc tataaagagg tcatcagtat atgaaacagc cccctgctgt
     3181 ccattcctta ttccatagaa aagccttgac ttgaggttag atttttttta tattttgttt
     3241 tgtgttattt ttttctttaa catccctaaa attttcctta catgttttac tagccagatt
     3301 tttcctcctc tcctgactac tcccagtcat agctgtccct cttctcttat gaagatccct
     3361 cgacttaatt aaggtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc
     3421 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
     3481 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
     3541 ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc
     3601 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     3661 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     3721 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     3781 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     3841 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     3901 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     3961 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct
     4021 tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     4081 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     4141 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     4201 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     4261 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     4321 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     4381 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     4441 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     4501 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     4561 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     4621 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     4681 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     4741 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     4801 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     4861 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     4921 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     4981 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     5041 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     5101 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     5161 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     5221 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     5281 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     5341 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     5401 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     5461 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     5521 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     5581 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     5641 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     5701 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     5761 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     5821 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     5881 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     5941 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     6001 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     6061 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
     6121 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
     6181 catgattacg ccaagcgcgc aattaaccct cactaaaggg aacaaaagct g
//
