LOCUS       sequence-390946-        6324 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression vector of mouse TSSK1. Myc-tag at N-terminus..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6324)
  AUTHORS   Shimada K, Park S, Oura S, Noda T, Morohoshi A, Matzuk MM, Ikawa M
  TITLE     TSKS localizes to nuage in spermatids and regulates cytoplasmic 
            elimination during spermiation.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Mar 14;120(11):e2221762120. doi: 
            10.1073/pnas.2221762120. Epub 2023 Mar 7.
  PUBMED    36881620
REFERENCE   2  (bases 1 to 6324)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-03-14"; volume: "120(11):e2221762120. 
            doi"; pages: " 10.1073/pnas.2221762120. Epub 2023 Mar 7"
COMMENT     Sequence Label: pCAG1.1-Myc/TSSK1
FEATURES             Location/Qualifiers
     source          1..6324
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        17..396
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        398..673
                     /label=chicken beta-actin promoter
     intron          674..1688
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1614..1636
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1696..1715
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1743..1752
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1752..1781
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(3004..3023)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3069..3124
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3123..3142)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(3479..3498)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3480..3498)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3508..3525)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3508..3524)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3517..3539)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3666..4121
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3753..3772)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3963..3984
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4147..4251
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4252..4320,4321..5112)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4470..4489)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5283..5871
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5772..5791
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6025..6042
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6159..6180
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6195..6200,6201..6218,6219..6225)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6233..6249
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6238..6260
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6257..6273
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6257..6273
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6292..6312
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6294..6312
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 gagctcatcg atactcgaca ttgattattg actagttatt aatagtaatc aattacgggg
       61 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg
      121 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
      181 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
      241 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
      301 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
      361 cagtacatct acgtattagt catcgctatt accatggtcg aggtgagccc cacgttctgc
      421 ttcactctcc ccatctcccc cccctcccca cccccaattt tgtatttatt tattttttaa
      481 ttattttgtg cagcgatggg ggcggggggg gggggggsgc gcgccaggcg gggcggggcg
      541 gggcgagggg cggggcgggg cgaggcggag aggtgcggcg gcagccaatc agagcggcgc
      601 gctccgaaag tttcctttta tggcgaggcg gcggcggcgg cggccctata aaaagcgaag
      661 cgcgcggcgg gcgggagtcg ctgcgcgctg ccttcgcccc gtgccccgct ccgccgccgc
      721 ctcgcgccgc ccgccccggc tctgactgac cgcgttactc ccacaggtga gcgggcggga
      781 cggcccttct cctccgggct gtaattagcg cttggtttaa tgacggcttg tttcttttct
      841 gtggctgcgt gaaagccttg aggggctccg ggagggccct ttgtgcgggg ggagcggctc
      901 ggggggtgcg tgcgtgtgtg tgtgcgtggg gagcgccgcg tgcggctccg cgctgcccgg
      961 cggctgtgag cgctgcgggc gcggcgcggg gctttgtgcg ctccgcagtg tgcgcgaggg
     1021 gagcgcggcc gggggcggtg ccccgcggtg cggggggggc tgcgagggga acaaaggctg
     1081 cgtgcggggt gtgtgcgtgg gggggtgagc agggggtgtg ggcgcgtcgg tcgggctgca
     1141 accccccctg cacccccctc cccgagttgc tgagcacggc ccggcttcgg gtgcggggct
     1201 ccgtacgggg cgtggcgcgg ggctcgccgt gccgggcggg gggtggcggc aggtgggggt
     1261 gccgggcggg gcggggccgc ctcgggccgg ggagggctcg ggggaggggc gcggcggccc
     1321 ccggagcgcc ggcggctgtc gaggcgcggc gagccgcagc cattgccttt tatggtaatc
     1381 gtgcgagagg gcgcagggac ttcctttgtc ccaaatctgt gcggagccga aatctgggag
     1441 gcgccgccgc accccctcta gcgggcgcgg ggcgaagcgg tgcggcgccg gcaggaagga
     1501 aatgggcggg gagggccttc gtgcgtcgcc gcgccgccgt ccccttctcc ctctccagcc
     1561 tcggggctgt ccgcgggggg acggctgcct tcggggggga cggggcaggg cggggttcgg
     1621 cttctggcgt gtgaccggcg gctctagcct ctgctaacca tgttcatgcc ttcttctttt
     1681 tcctacagct cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaatcta
     1741 gcgccgccat ggaacaaaaa ctcatctcag aagaggatct ggggggcggg tctagaggat
     1801 ccctgcagga attcatggat gacgctgccg tcctcaagcg acgaggctac atcatgggga
     1861 taaacttggg agagggctca tatgccaaag tcaaatccgc ttactctgag cgcctaaagt
     1921 tcaacgtggc ggtcaagatc atcgatcgca agaaagcccc ctcagacttc ctggagaaat
     1981 tccttccccg ggagattgag attctggcca tgctaaacca ccgctctatt gtcaagacct
     2041 atgagatctt tgagacatca gatggcaagg tctacattgt catggagctt ggggtccagg
     2101 gtgacctcct cgagttcatc aaaaccagag gagccctgca agaggatgat gctcgaaaga
     2161 agttccacca gctctcctcg gccatcaagt actgccacga cctggatgtc gtccaccgag
     2221 acctcaaatg tgagaacctt ctgcttgaca aggacttcaa catcaaactg tctgacttcg
     2281 gcttctccaa gcgctgcttg cgggatgata gtggtcgact gatattaagc aagaccttct
     2341 gtgggtcagc agcttatgcg gcccccgagg tgctgcaggg catcccctac cagcccaagg
     2401 tgtatgacat ctggagcctg ggtgtgattc tctacatcat ggtctgtggc tccatgccct
     2461 acgatgactc caacatcaaa aagatgctgc gcatccagaa ggagcaccgt gtcaactttc
     2521 cacgctctaa acacctgact ggtgagtgca aggacctcat ctacagaatg ttacagccag
     2581 atgtcaaccg ccggctgcac atcgatgaga tccttaacca ctgctgggtg cagcccaagg
     2641 cacgaggtct gtcctctgga gccatcaaca aggaggggga aagctcccgg gccactgagc
     2701 cctcatggat ccctgaacct ggtgctgata agaagtctgc caccaagttg gagcctaggg
     2761 aagaggcacg atctgaggcc cggtctgagt caaaacccca ggaagatact ttgcaggtgg
     2821 tcaggcagtc agagaacgtg ggcctcagca gcgaactgaa cagggacaca gaggaagggc
     2881 acccccaaca gccttcagag acacatactt aggcggccgc ctcgagactc ctcaggtgca
     2941 ggctgcctat cagaaggtgg tggctggtgt ggccaatgcc ctggctcaca aataccactg
     3001 agatcttttt ccctctgcca aaaattatgg ggacatcatg aagccccttg agcatctgac
     3061 ttctggctaa taaaggaaat ttattttcat tgcaatagtg tgttggaatt ttttgtgtct
     3121 ctcactcgga aggacatatg ggagggcaaa tcatttaaaa catcagaatg agtatttggt
     3181 ttagagtttg gcaacatatg cccatatgct ggctgccatg aacaaaggtt ggctataaag
     3241 aggtcatcag tatatgaaac agccccctgc tgtccattcc ttattccata gaaaagcctt
     3301 gacttgaggt tagatttttt ttatattttg ttttgtgtta tttttttctt taacatccct
     3361 aaaattttcc ttacatgttt tactagccag atttttcctc ctctcctgac tactcccagt
     3421 catagctgtc cctcttctct tatgaagatc cctcgactta attaaggtac ccaattcgcc
     3481 ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga
     3541 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
     3601 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
     3661 atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     3721 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     3781 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     3841 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     3901 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     3961 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     4021 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     4081 atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga
     4141 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     4201 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     4261 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     4321 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     4381 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     4441 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     4501 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     4561 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     4621 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     4681 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     4741 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     4801 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     4861 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     4921 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     4981 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     5041 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     5101 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     5161 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     5221 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     5281 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     5341 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     5401 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     5461 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     5521 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     5581 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     5641 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     5701 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     5761 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     5821 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     5881 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     5941 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     6001 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     6061 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
     6121 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
     6181 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
     6241 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac
     6301 cctcactaaa gggaacaaaa gctg
//
