LOCUS       sequence-39096-e         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Vick JE, Johnson ET, Choudhary S, Bloch SE, Lopez-Gallego F, 
            Srivastava P, Tikh IB, Wawrzyn GT, Schmidt-Dannert C
  TITLE     Optimized compatible set of BioBrick vectors for metabolic pathway 
            engineering.
  JOURNAL   Appl Microbiol Biotechnol. 2011 Dec;92(6):1275-86. Epub 2011 Oct 28.
  PUBMED    22033566
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Microbiol Biotechnol."; date: "2011-12"; volume: "92(6)"; pages: 
            "1275-86. Epub 2011 Oct 28"
COMMENT     Sequence Label: pBBRBB-eGFP
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(11..28)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     regulatory      38..47
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(99..120)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 aggagaatca gtgatggtga tggtgatgct cgaggcggcc gccatggatg catttacttg
       61 tacagctcgt ccatgccgag agtgatcccg gcggcggtca cgaactccag caggaccatg
      121 tgatcgcgct tctcgttggg gtctttgctc agggcggact gggtgctcag gtagtggttg
      181 tcgggcagca gcacggggcc ntcgccgatg ggggtgttct tctgnntggt agtggtccgg
      241 naaatttcaa cgccngccnc ncctcnattt tggggggcna aatcttnnaa tttcaccttt
      301 gangncnntt tnttctgntt ngnncngnaa naanaaaaaa cnttggnggc tgttgaaaat
      361 tntacccncc ctttggcccc naaaaaatgt nccccccnta aaaaaaaaac accccccnan
      421 anntngangc ggttcaccag ggtgtcgccc tcgaacttca cctcggcgcg ggtcttgtan
      481 ttgccgtcgt ccttgaagaa gatggtgcgc tcctggacgt anccttcggg catggcggac
      541 ttgaagaagt cgtgctgctt catggangtc ggggtagcgg ctgaagcact gcacgccgta
      601 ngtcagggtg gtcacgaggg tgggccaggg cacgggcagc ttgccggtgg tgcagatgaa
      661 cttcanggtc agcttgccgt aagtggcatc gccctcgccc tcgccggaca cgctgaactt
      721 gtggccgttt acgtcgccgt ccagctcgac cangatgggc accaccccgg tgaacagctc
      781 ctcgcccttg ctcaccatat
//
