LOCUS       sequence-390985-        5912 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Drives specific expression in the ASH neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5912)
  AUTHORS   Nava S, Palma W, Wan X, Oh JY, Gharib S, Wang H, Revanna JS, Tan M, 
            Zhang M, Liu J, Chen CH, Lee JS, Perry B, Sternberg PW
  TITLE     A cGAL-UAS bipartite expression toolkit for Caenorhabditis elegans 
            sensory neurons.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Dec 19;120(51):e2221680120. doi: 
            10.1073/pnas.2221680120. Epub 2023 Dec 14.
  PUBMED    38096407
REFERENCE   2  (bases 1 to 5912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-12-19"; volume: "120(51):e2221680120. 
            doi"; pages: " 10.1073/pnas.2221680120. Epub 2023 Dec 14"
COMMENT     Sequence Label: pMZ41
FEATURES             Location/Qualifiers
     source          1..5912
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2970..3119
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     promoter        3657..3761
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3762..3830,3831..4622)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3980..3999)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4793..5381
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5282..5301
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5535..5552
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5669..5690
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5705..5710,5711..5728,5729..5735)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5743..5759
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5748..5770
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5767..5783
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5767..5783
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgcat gcgcggccgc actgactggg ccggccaata aatgacgttc tcgttccaat
       61 aagaagaatg cgactaacaa aagttgaata tgttttgctg caagcaatta ttttcttcga
      121 tcctggttag tgcagttttg aaatttctca aaactatgta gtgctttcaa acacacaaat
      181 tggccctttt aagagaaaca aatacaacaa aacaaataca acaaaaaata gttcaaaact
      241 tcgccttcca gttttcacat tgaactgaac tgttaaatct taaatatact tgatttccct
      301 aattgtattt tagtgtgctg aaaactagtt ggaagtgtta tatgctggag taaaagtaga
      361 aaaacgtttc attattttaa tgtttgaaag cagaaaaaac tcccatgtct ttttttcaaa
      421 aatgtttttc ttcagtaagt tagcatattc agaatacatt tctgaaaatt aagtatgtag
      481 ctttcctaca catgtttttg aaaaacagtt tatattgaaa gcagttaaat gcttataagg
      541 tcaattccat aattgaaaaa ttgaaacttt acttgatagg acaaagatct gtgtcaatta
      601 acgtcttgca caaaataata aaaagaaatc cgtcgaaccg gattcgaacc agtgacctaa
      661 ggatacctgc tgactctaca gtcctccgct ctaccaactg agctatcgac ggcacgagga
      721 agcaggagag cgacagcggt tatatagagg acattctcgc tttcactctc gcttctctgc
      781 ccctatgtct atttagtaga cgcagagttt tgccacatgt attctctgcg ttttcaaatt
      841 gctcatgctt tgggttggca aataaagtta tgcacatgtt tatgaatttt aggagaaatt
      901 tgcccaggtg gcatgacaaa tcttattgcg aaaggttact taactagatt tcacggaagt
      961 ttgattaagt taaaaattat cccacatata tctaatacaa atgtattgat aagtacgaaa
     1021 atagaaacac agcacatcat ttttttaaac gaaaatgaaa aaaaaaaaaa gttacggaat
     1081 ataaattaag gttcataagt gacgtgattc agtgttacgt aaactatgtg tatttgtttc
     1141 caaaatgatt ttttaaacag aaccgtataa caaatttctc aattttcaga ttgtctttcc
     1201 ctgacaaaac acggctccca actcattgct gccaaacgca agcgacttct ggattccctt
     1261 caaaaatggc ttcatcaaca aacgaaagac tcaattgaag cgtccaccag atttgcggaa
     1321 atcctcctca gaatctgtaa tgtacaggtg agaatattct gccccattgt tcttcaaaat
     1381 tcgtaataaa aacaaaacga aatgaagcta aaagagaaaa acaaaaatct taactttaca
     1441 aaaattcatt gtgactcgtc ctacacaatt cgacgctcac ttgactgatt ttgaggcaaa
     1501 atttaatttt taagaattta ataatgagaa ggaaaaattg aattgcagga cctttcacta
     1561 aaaaaccgag ttttatttca gaaagtggcg gcattcaaac gagaaacact gtgcacaatt
     1621 gagacattcg aattgatgca gccacatccg ttcaccatgg aaatttctaa aagttatccc
     1681 gacttttcat atttctgaaa atttacatat ttatgatgca acttacaacc agcttctagt
     1741 cactttaagc tgatttctag acaatagtca ttgcaaccct acagtagata ctataaatgt
     1801 ttttatttcc cctttccact acctcatgtt agtttttata atcactatgc tcacgtgtca
     1861 agaaactttc ggaacgtttc gattcgattt ttaatccgtt ggtgcgaaat tcatgttctc
     1921 ccattactcc tttcgtttct tttttgatca tttttgcgcg ggtatgtttt ccttctaaac
     1981 gttcctaatt tcatcgaatc aatgttttaa ttgttcagta tatagacgat atgttgccaa
     2041 acaagtaccc ccatacctat ttcgtcttct agcactttct catatcagta taaagctgag
     2101 agtcttgtga taaacgacac aattcaataa agacaagggt ttccacagca gcgcgcacgt
     2161 ttccgcgcta attcgattca caggtcgtca atactaccgt agtgaatgac gagcaatgct
     2221 gaaatttgat tattattaca ctggaactga gaaaattggt aaataggcgg agcttgcatg
     2281 gctcggccca ccttttggag ccgagcttgg tcattcgaca tttgagctgt cagcaagggc
     2341 gcgcctctag aggatccccg ggattggcca aaggacccaa aggtatgttt cgaatgatac
     2401 taacataaca tagaacattt tcaggaggac ccttggctag gatgggccct aaaaagaagc
     2461 gtaaagctag catgaagctg ttgtcttcaa tggaacaagc ttgcgacata tgccggttaa
     2521 aaaaactcag gtgctcgaag gaaaagccca agtgtgccaa gtgtctgaag aacaactcgg
     2581 agtgccgtta ttctcccaag gctatgaggt ccccactgac cagggcgcat ctgacagagg
     2641 tagaatcaag gttggaaaaa ttggaagatt tgtttctgct catgtttcct cgggaaaacc
     2701 tggacagcat tttgaacatg gactctttgg acgaagtgaa agtaatgttg aaacaattat
     2761 atatgcagga ctatggtgac acggatgctg ctatggatag cttgacccct atggaaaatg
     2821 gcatgtctga agggccgaga aaacacatga agactgcgac atcatcgcca aaagtggatg
     2881 aagatggaag ccaaagtcag ttgagtgtat cgccggaatt cccgctgcag tctggtggcg
     2941 gaggggctcc taggggttcc ggacgggctg acgcattgga cgattttgat ctggatatgc
     3001 tgggaagtga cgccctcgat gattttgacc ttgacatgct tggttcggat gcccttgatg
     3061 actttgacct cgacatgctc ggcagtgacg cccttgatga tttcgacctg gacatgctga
     3121 ttaactaagg taccgaattc gctagccggc catacaagta atccggatga tcgacgccaa
     3181 cgtcgttgaa ttttcaaatt ttaaatactg aatatttgtt ttttttccta ttatttattt
     3241 attctctttg tgtttttttt cttgctttct aaaaaattaa ttcaatccaa atctaaacat
     3301 ttttttttct ctttccgtct cccaattcgt attccgctcc tctcatctga acacaatgtg
     3361 caagtttatt tatcttctcg ctttcatttc attaggacgt ggggggaatt ggtggaaggg
     3421 ggaaacacac aaaaggatga tggaaatgaa ataaggacac acaatatgca acaacattca
     3481 attcagaaat atggaggaag gtttaaaaga aaacataaaa atatatagag gaggaaggaa
     3541 aactagtaaa aaataagcaa agaaattagg cgaacgatga gaattgtcct cgcttgggcc
     3601 caaaaggcct tttttttttg gcgccgacgt caggtggcac ttttcgggga aatgtgcgcg
     3661 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     3721 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     3781 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     3841 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     3901 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     3961 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     4021 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     4081 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     4141 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     4201 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     4261 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     4321 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     4381 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     4441 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     4501 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     4561 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     4621 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     4681 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     4741 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     4801 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     4861 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     4921 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     4981 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     5041 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     5101 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     5161 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     5221 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     5281 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     5341 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     5401 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     5461 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     5521 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
     5581 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
     5641 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
     5701 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
     5761 ttcacacagg aaacagctat gaccatgatt acgccaagct gtaagtttaa acatgatctt
     5821 actaactaac tattctcgtt taaattttca gagcttaaaa atggctgaaa tcactcacaa
     5881 cgatggatac gctaacaact tggaaatgaa at
//
