LOCUS       sequence-390993-        6016 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  In vivo neuronal inhibition through histamine-gated chloride 
            channel. Neurons expressing HisCl1 transgene are inhibited after 
            addition of histamine..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6016)
  AUTHORS   Nava S, Palma W, Wan X, Oh JY, Gharib S, Wang H, Revanna JS, Tan M, 
            Zhang M, Liu J, Chen CH, Lee JS, Perry B, Sternberg PW
  TITLE     A cGAL-UAS bipartite expression toolkit for Caenorhabditis elegans 
            sensory neurons.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Dec 19;120(51):e2221680120. doi: 
            10.1073/pnas.2221680120. Epub 2023 Dec 14.
  PUBMED    38096407
REFERENCE   2  (bases 1 to 6016)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-12-19"; volume: "120(51):e2221680120. 
            doi"; pages: " 10.1073/pnas.2221680120. Epub 2023 Dec 14"
COMMENT     Sequence Label: pJL046
FEATURES             Location/Qualifiers
     source          1..6016
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    36..130
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    169..263
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    302..396
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     primer_bind     complement(2399..2427)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     3175..3195
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     promoter        3761..3865
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3866..3934,3935..4726)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4084..4103)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4897..5485
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5386..5405
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5639..5656
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5773..5794
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5809..5814,5815..5832,5833..5839)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5847..5863
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5852..5874
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5871..5887
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5871..5887
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgcta gcccatgatt acgccaagct acgggcggag tactgtcctc cgagcggagt
       61 actgtcctcc gagcggagta ctgtcctccg agcggagtac tgtcctccga gcggagtact
      121 gtcctccgag cggagactcc ctagcccatg attacgccaa gctacgggcg gagtactgtc
      181 ctccgagcgg agtactgtcc tccgagcgga gtactgtcct ccgagcggag tactgtcctc
      241 cgagcggagt actgtcctcc gagcggagac tccctagccc atgattacgc caagctacgg
      301 gcggagtact gtcctccgag cggagtactg tcctccgagc ggagtactgt cctccgagcg
      361 gagtactgtc ctccgagcgg agtactgtcc tccgagcgga gactccctag gggcggccgc
      421 actgactggg ccggccaaaa atgcataagg ttttgctggc actgttcttt atctttctgg
      481 caccagcatc cgcactggca gtctccgaac cggcctgcag gtcgattttt tgcaaattac
      541 gagcgttgta gggggcggag cgataggtcc tataggtttt ggtatatcat cattcattca
      601 ttcattggta cattcattta cccaccttcc tctttctgag cttctctgga gttctgtgct
      661 tcctttttcc cttatcttta tactgtaatt tttaactttc aggcattgat tggcgcgcct
      721 ctagaggatc cccgggattg gccaaaggac ccaaaggtat gtttcgaatg atactaacat
      781 aacatagaac attttcagga ggacccttgg ctagcgtcga cggtaccggt aaaaatgcaa
      841 agcccaacta gcaaattggt agaattcagg tgcctgattg cgttggcaat atatttgcac
      901 gcgctggagc aatcgatcca gcactgccat tgtgttcatg gttacagaaa taacacggag
      961 agcgccgagc tggtctccca ctacgagtcg agtctttcgc tcccggacat tttgcccatt
     1021 ccctcaaaga cgtacgacaa gaaccgggct cccaagctcc tcggccagcc cacagtagtc
     1081 tacttccatg tcacggtcct ctccctggac tccattaacg aggagtctat gacctatgtg
     1141 acggacatct tccttgcaca aagctggcgt gatcctcgcc tgcggttgcc tgagaacatg
     1201 agtgagcagt atcgcatatt ggatgtcgac tggttgcaca gcatttggcg gcccgattgc
     1261 ttctttaaga acgccaaaaa ggtcaccttc catgagatga gcattcccaa tcactatctc
     1321 tggctgtacc acgacaaaac gctgctctat atgtccaaac tcacgttggt cctgtcgtgc
     1381 gccatgaagt ttgagtccta tccgcatgac acgcaaatct gctccatgat gatcgagagt
     1441 ttatcccata cggtggaaga tttggttttc atttggaaca tgaccgaccc acttgtggtt
     1501 aacacggaga ttgagttgcc gcagctagac atatcaaata actacacaac cgactgtact
     1561 atagagtact caacaggtaa cttcacctgc ctggccattg tgttcaacct gcgccgacgc
     1621 ctgggttacc atttgttcca cacctacatc ccctcggctc tgattgtggt catgtcttgg
     1681 atatcgtttt ggataaaacc agaagcgata cccgcccgtg taactctggg agtgacctca
     1741 ctgctaaccc tggccaccca gaatacccag tcgcaacaat cgctgccgcc ggtttcgtat
     1801 gtcaaggcta tagacgtctg gatgtcgtcc tgttcggtgt ttgtattcct ttctctgatg
     1861 gaatttgcag tggtcaacaa ttttatggga ccggtggcca caaaggcaat gaaggggtat
     1921 tcggacgaga acatcagtga tctggacgac ctaaagcatc atcgggaatc gattattgag
     1981 ccccagtacg acactttctg ccatggccat gccacagcca tttatataga caaattctcg
     2041 cgctttttct tcccgttttc gttctttata ctcaatattg tctattggac aacgttccta
     2101 tgaggtaccg tagctgtctc atcctacttt cacctagtta actgcttgtc ttaaaatcta
     2161 tgcttctctt tagtatctaa aattttccta gaagcttaca agtatataaa tggtctcttc
     2221 tcaataaagg ttgtatattt attcatctta ttgaatctgc catttcctcg tttttgcgag
     2281 tttatatacc ttccaatttt ctttctattg tattttcaac ttctaatttt aattcaggga
     2341 aactgctgta ccggtagaaa aaatgctcga gagtaaagga gaagaacttt tcactggagt
     2401 tgtcccaatt cttgttgaat tagatggtga tgttaatggg cacaaatttt ctgtcagtgg
     2461 agagggtgaa ggtgatgcaa catacggaaa acttaccctt aaatttattt gcactactgg
     2521 aaaactacct gttccatggg taagtttaaa catatatata ctaactaacc ctgattattt
     2581 aaattttcag ccaacacttg tcactacttt ctgttatggt gttcaatgct tctcgagata
     2641 cccagatcat atgaaacggc atgacttttt caagagtgcc atgcccgaag gttatgtaca
     2701 ggaaagaact atatttttca aagatgacgg gaactacaag acacgtaagt ttaaacagtt
     2761 cggtactaac taaccataca tatttaaatt ttcaggtgct gaagtcaagt ttgaaggtga
     2821 tacccttgtt aatagaatcg agttaaaagg tattgatttt aaagaagatg gaaacattct
     2881 tggacacaaa ttggaataca actataactc acacaatgta tacatcatgg cagacaaaca
     2941 aaagaatgga atcaaagttg taagtttaaa catgatttta ctaactaact aatctgattt
     3001 aaattttcag aacttcaaaa ttagacacaa cattgaagat ggaagcgttc aactagcaga
     3061 ccattatcaa caaaatactc caattggcga tggccctgtc cttttaccag acaaccatta
     3121 cctgtccaca caatctgccc tttcgaaaga tcccaacgaa aagagagacc acatggtcct
     3181 tcttgagttt gtaacagctg ctgggattac acatggcatg gatgaactat acaaatagga
     3241 attcgctagc cggccataca agtaatccgg atgatcgacg ccaacgtcgt tgaattttca
     3301 aattttaaat actgaatatt tgtttttttt cctattattt atttattctc tttgtgtttt
     3361 ttttcttgct ttctaaaaaa ttaattcaat ccaaatctaa acattttttt ttctctttcc
     3421 gtctcccaat tcgtattccg ctcctctcat ctgaacacaa tgtgcaagtt tatttatctt
     3481 ctcgctttca tttcattagg acgtgggggg aattggtgga agggggaaac acacaaaagg
     3541 atgatggaaa tgaaataagg acacacaata tgcaacaaca ttcaattcag aaatatggag
     3601 gaaggtttaa aagaaaacat aaaaatatat agaggaggaa ggaaaactag taaaaaataa
     3661 gcaaagaaat taggcgaacg atgagaattg tcctcgcttg ggcccaaaag gccttttttt
     3721 tttggcgccg acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     3781 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     3841 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     3901 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     3961 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     4021 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     4081 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     4141 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     4201 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     4261 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     4321 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     4381 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     4441 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     4501 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     4561 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     4621 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     4681 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     4741 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     4801 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     4861 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     4921 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     4981 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     5041 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     5101 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     5161 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     5221 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     5281 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     5341 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     5401 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     5461 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     5521 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     5581 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     5641 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
     5701 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
     5761 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
     5821 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
     5881 ctatgaccat gattacgcca agctgtaagt ttaaacatga tcttactaac taactattct
     5941 catttaaatt ttcagagctt aaaaatggct gaaatcactc acaacgatgg atacgctaac
     6001 aacttggaaa tgaaat
//
