LOCUS       sequence-391007-        6512 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Cre-ERT2 under the control of c-Fos promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6512)
  AUTHORS   Lee JH, Kim WB, Park EH, Cho JH
  TITLE     Neocortical synaptic engrams for remote contextual memories.
  JOURNAL   Nat Neurosci. 2022 Dec 23. doi: 10.1038/s41593-022-01223-1.
  PUBMED    36564546
REFERENCE   2  (bases 1 to 6512)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2022 Dec 23. doi: 10.1038/s41593-022-01223-1."
COMMENT     Sequence Label: pAAV-Fos-CreERT2
FEATURES             Location/Qualifiers
     source          1..6512
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..141
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   12..141
                     /label=AAV2 ITR
     protein_bind    148..168
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             complement(868..879)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     protein_bind    888..912
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             919..1947
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     CDS             1960..2895
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             2008..2901
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPVKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATA"
     protein_bind    complement(2902..2926)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    2936..3524
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2989..3009)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3407..3418)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3554..3571)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3560..3767
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   3775..3915
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3775..3904
                     /label=AAV2 ITR
     rep_origin      3990..4445
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4077..4096)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4287..4308
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4462..4481)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4581..4603
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4641..4659)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4727..4831
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4832..4900,4901..5692)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5050..5069)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5863..6451
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6352..6371
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc ttctagacaa ctttgtatag aaaagttgag ctttccttta
      181 ggaacagagg cttcgagcct ttaaggctgc gtacttgctt ctcctaatac cagagactca
      241 aaaaaaaaaa aaaagttcca gattgctgga caatgacccg ggtctcatcc cttgaccctg
      301 ggaaccgggt ccacattgaa tcaggtgcga atgttcgctc gccttctctg cctttcccgc
      361 ctcccctccc ccggccgcgg ccccggttcc ccccctgcgc tgcaccctca gagttggctg
      421 cagccggcga gctgttcccg tcaatccctc cctcctttac acaggatgtc catattagga
      481 catctgcgtc agcaggtttc cacggccggt ccctgttgtt ctgggggggg gaccatctcc
      541 gaaatcctac acgcggaagg tctaggagac cccctaagat cccaaatgtg aacactcata
      601 ggtgaaagat gtatgccaag acgggggttg aaagcctggg gcgtagagtt gacgacagag
      661 cgcccgcaga gggccttggg gcgcgcttcc ccccccttcc agttccgccc agtgacgtag
      721 gaagtccatc cattcacagc gcttctataa aggcgccagc tgaggcgcct actactccaa
      781 ccgcgactgc agcgagcaac tgagaagact ggatagagcc ggcggttccg cgaacgagca
      841 gtgaccgcgc tcccacccag ctctgctctg cagctcccac cagtgtctca agtttgtaca
      901 aaaaagcagg ctgccaccat gtccaattta ctgaccgtac accaaaattt gcctgcatta
      961 ccggtcgatg caacgagtga tgaggttcgc aagaacctga tggacatgtt cagggatcgc
     1021 caggcgtttt ctgagcatac ctggaaaatg cttctgtccg tttgccggtc gtgggcggca
     1081 tggtgcaagt tgaataaccg gaaatggttt cccgcagaac ctgaagatgt tcgcgattat
     1141 cttctatatc ttcaggcgcg cggtctggca gtaaaaacta tccagcaaca tttgggccag
     1201 ctaaacatgc ttcatcgtcg gtccgggctg ccacgaccaa gtgacagcaa tgctgtttca
     1261 ctggttatgc ggcggatccg aaaagaaaac gttgatgccg gtgaacgtgc aaaacaggct
     1321 ctagcgttcg aacgcactga tttcgaccag gttcgttcac tcatggaaaa tagcgatcgc
     1381 tgccaggata tacgtaatct ggcatttctg gggattgctt ataacaccct gttacgtata
     1441 gccgaaattg ccaggatcag ggttaaagat atctcacgta ctgacggtgg gagaatgtta
     1501 atccatattg gcagaacgaa aacgctggtt agcaccgcag gtgtagagaa ggcacttagc
     1561 ctgggggtaa ctaaactggt cgagcgatgg atttccgtct ctggtgtagc tgatgatccg
     1621 aataactacc tgttttgccg ggtcagaaaa aatggtgttg ccgcgccatc tgccaccagc
     1681 cagctatcaa ctcgcgccct ggaagggatt tttgaagcaa ctcatcgatt gatttacggc
     1741 gctaaggatg actctggtca gagatacctg gcctggtctg gacacagtgc ccgtgtcgga
     1801 gccgcgcgag atatggcccg cgctggagtt tcaataccgg agatcatgca agctggtggc
     1861 tggaccaatg taaatattgt catgaactat atccgtaacc tggatagtga aacaggggca
     1921 atggtgcgcc tgctggaaga tggcgatctc gagccatctg ctggagacat gagagctgcc
     1981 aacctttggc caagcccgct catgatcaaa cgctctaaga agaacagcct ggccttgtcc
     2041 ctgacggccg accagatggt cagtgccttg ttggatgctg agccccccat actctattcc
     2101 gagtatgatc ctaccagacc cttcagtgaa gcttcgatga tgggcttact gaccaacctg
     2161 gcagacaggg agctggttca catgatcaac tgggcgaaga gggtgccagg ctttgtggat
     2221 ttgaccctcc atgatcaggt ccaccttcta gaatgtgcct ggctagagat cctgatgatt
     2281 ggtctcgtct ggcgctccat ggagcaccca gtgaagctac tgtttgctcc taacttgctc
     2341 ttggacagga accagggaaa atgtgtagag ggcatggtgg agatcttcga catgctgctg
     2401 gctacatcat ctcggttccg catgatgaat ctgcagggag aggagtttgt gtgcctcaaa
     2461 tctattattt tgcttaattc tggagtgtac acatttctgt ccagcaccct gaagtctctg
     2521 gaagagaagg accatatcca ccgagtcctg gacaagatca cagacacttt gatccacctg
     2581 atggccaagg caggcctgac cctgcagcag cagcaccagc ggctggccca gctcctcctc
     2641 atcctctccc acatcaggca catgagtaac aaaggcatgg agcatctgta cagcatgaag
     2701 tgcaagaacg tggtgcccct ctatgacctg ctgctggagg cggcggacgc ccaccgccta
     2761 catgcgccca ctagccgtgg aggggcatcc gtggaggaga cggaccaaag ccacttggcc
     2821 actgcgggct ctacttcatc gcattccttg caaaagtatt acatcacggg ggaggcagag
     2881 ggtttccctg ccacagcttg aacccagctt tcttgtacaa agtgggaatt ccgataatca
     2941 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     3001 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     3061 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     3121 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     3181 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     3241 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     3301 cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg
     3361 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     3421 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
     3481 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcggg aattcctaga
     3541 gctcgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc
     3601 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
     3661 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
     3721 gacagcaagg gggaggattg ggaagagaat agcaggcatg ctggggaggg ccgcaggaac
     3781 ccctagtgat ggagttggcc actccctctc tgcgcgctcg ctcgctcact gaggccgggc
     3841 gaccaaaggt cgcccgacgc ccgggctttg cccgggcggc ctcagtgagc gagcgagcgc
     3901 gcagctgcct gcaggggcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt
     3961 cacaccgcat acgtcaaagc aaccatagta cgcgccctgt agcggcgcat taagcgcggc
     4021 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccttag cgcccgctcc
     4081 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     4141 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     4201 tgatttgggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     4261 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     4321 ctctatctcg ggctattctt ttgatttata agggattttg ccgatttcgg tctattggtt
     4381 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgtttac
     4441 aattttatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg
     4501 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta
     4561 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc
     4621 gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
     4681 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     4741 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     4801 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     4861 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     4921 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     4981 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     5041 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     5101 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     5161 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     5221 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     5281 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     5341 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     5401 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     5461 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     5521 tgataaatct ggagccggtg agcgtggaag ccgcggtatc attgcagcac tggggccaga
     5581 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     5641 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     5701 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     5761 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     5821 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     5881 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     5941 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     6001 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     6061 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     6121 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     6181 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     6241 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     6301 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     6361 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     6421 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     6481 cctggccttt tgctggcctt ttgctcacat gt
//
