LOCUS       sequence-39101-e         704 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Erdmann S, Senkel S, Arndt T, Lucas B, Lausen J, Klein-Hitpass L, 
            Ryffel GU, Thomas H
  TITLE     Tissue-specific transcription factor HNF4alpha inhibits cell 
            proliferation and induces apoptosis in the pancreatic INS-1 
            beta-cell line.
  JOURNAL   Biol Chem. 2007 Jan . 388(1):91-106.
  PUBMED    17214554
REFERENCE   2  (bases 1 to 704)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biol Chem. 
            2007 Jan . 388(1):91-106."
COMMENT     Sequence Label: FR_GFP
FEATURES             Location/Qualifiers
     source          1..704
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(84..102)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(85..102)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(194..218)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    228..246
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    249..267
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        complement(269..472)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(298..318)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 gaattgggac aactccagtg aaaagttctt ctcctttact catggtggct gtggatcctg
       61 caaaaagaac aagtagcttg tattctatag tgtcacctaa atcaagctta agtttaaacg
      121 ctagagtccg gaggctggat cggtcccggt gtcttctatg gaggtcaaaa cagcgtggat
      181 ggcgtctcca ggcgatctga cggttcacta aacgagctcg tcgacgatct ctatcactga
      241 tagggagatc tctatcactg atagggagag ctctgcttat atagacctcc caccgtacac
      301 gcctaccgcc catttgcgtc aatggggcgg agttgttacg acattttgga aagtcccgtt
      361 gattttggtg ccaaaacaaa ctcccattga cgtcaatggg gtggagactt ggaaatcccc
      421 gtgagtcaaa ccgctatcca cgcccattga tgtactgcca aaaccgcatc accatggtaa
      481 tagcgatgac taatacgtag atgtactgcc aagtaggaaa gtcccataag gtcatgtact
      541 gggcataatg ccaggcgggc catttaccgt cattgacgtc aatagggggc gtacttggca
      601 tatgatacac ttgatgtact gccaagtggg cagtttaccg taaatactcc acccattgac
      661 gtcaatggaa agtccctatt ggcgttacta tgggaacata cgtc
//
