LOCUS       sequence-391016-        4255 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobrick (MoClo) Level 0 Part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4255)
  AUTHORS   Chamness JC, Kumar J, Cruz AJ, Rhuby E, Holum MJ, Cody JP, Tibebu R,
            Gamo ME, Starker CG, Zhang F, Voytas DF
  TITLE     An extensible vector toolkit and parts library for advanced 
            engineering of plant genomes.
  JOURNAL   Plant Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312.
  PUBMED    36896468
REFERENCE   2  (bases 1 to 4255)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312."
COMMENT     Sequence Label: pMC-1-TBS-11
FEATURES             Location/Qualifiers
     source          1..4255
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(2135..2153)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2221..2325
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2547..3338
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3509..4097
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3998..4017
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(4251..4255,1..13)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
       61 gccgattcat taatcactct gtggtctcag gagaaccttt tatgtacttc acaaccctca
      121 atgctgcttc caggtgtgac tgtttgggtt gctgcatgaa ttgactcagc agctgcactg
      181 caaagctgat atctggcctt gtgattgtca agtagagaag ctttcccacc agtctctgat
      241 aggatcccac atcctctaac tctgcatcat cagtctttcc taagtgcttg tcatattcta
      301 cagtagtcag cctttggttc tgttccattg gggttgacac tggtctgcag cctcccagac
      361 caacacctga tatcagttcc agtgcatact tcctctggtt cagtaggatt cccttttctg
      421 atctcagcac ctcaatgcct aagaagtatt ttagttctcc caaatctttc atcttgaaat
      481 gctgatgcag ggttgccttt gcttctgaaa tcaaaacatt gctgctgcct gttattaaca
      541 gatcatccac ataaatcagg attatgacaa ggtcagtccc ctctcttttg gtaaacaagg
      601 agtgatcata agcactttgc ataaaaccag cctgcataag gacagtggta agcttgatgt
      661 tccactgcct tgatgcttgc tttaaaccat agagggattc aacagcctgc acactttggt
      721 gtccccttgg ctgtgaaaac cctgaggcag agacatataa acttcttcca tgaggtcacc
      781 ttgtagaaaa gcattgttga catccatctg gaaaaggaac cagcccttgg aagcagcaac
      841 agatatgaca gcttttacag tgaccatttt ggccactgga gaaaaagttt catggtagtc
      901 aaggccttct tgctgagtgt atcccttggc cactagcctt gccttaaacc tgtcaacttc
      961 accattagct ttgtatttaa ttttgtacac ccatttggac cctataggct gtttaccagg
     1021 gggtaaaggg acaatctccc aggtgttatt atcctcaaga gcctgtatct caagggacat
     1081 ggcctccatc catttctcat cttgagctgc ttctttgaag gatttaggtt cagtataagt
     1141 ggagaaagca ctcaaataag cttgatagtg aacaggcaag tgatcatagg aaacatagtt
     1201 ggctataggg tatggaacat ccctagagcc tttgttcagt gtcacaaagt ccttgagcca
     1261 gatgggagga cctgcattgc gtttaggtct agtgtgcagg tttgctggaa ctagagaggg
     1321 atcagctaca gcagtatggt gctcaaattc agcatttgct aggtcaggct cagctgactc
     1381 aactgactct gcaggagcat gcacgtggcc tgaaggtgca gcatcagctg aagtgatatg
     1441 attttgggta gaatgagtcc ccaaagtgat atcctcattg gcctctacaa tgtcaggaat
     1501 aagaactgga gtatcatcat catcagtatg aaagctggaa ggaaaaatat cattatacac
     1561 tagctgcaaa gctgtatctt cagagctaag tgaacctgct cgagtgaaca tatcaggttc
     1621 atgggagatg gactctttga aagggaactg aaactctctg aaaactacat ccctgctcac
     1681 tatgatcacc ttattatcca agtcatacaa cctgtaaccc ttttgagtct cagaataacc
     1741 aatgaagatg gttcgcttgg ctctaggtgc aagtttgtca cctttgggca gtgttgctgc
     1801 aaaagcaaga cacccaaaca ctctcaagtg atcaagcttg gcatgtttct gatagagtag
     1861 ttcatatgga catttgcctt gtaagattgg agtagggagt ctattgatca ggtatacaac
     1921 agtcttgaca cagtctcccc aaaacctggt aggtacacca ctctgaaact taagtgccct
     1981 tgccatctca aggatgtgtc tgtgctttct ctccacaaca ccattctgtt gtggtgtgta
     2041 gggacagcta ctttgatgaa caatcccaag agaggccagc aactcattac aacttcgctt
     2101 gagaccacga agtggctctt cagtggacga aagggcctcg tgatacgcct atttttatag
     2161 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
     2221 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     2281 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgcg ctcacgcaac
     2341 tggtccagaa ccttgaccga acgcagcggt ggtaacggcg cagtggcggt tttcatggct
     2401 tgttatgact gttttttttt ggggtacagt ctatgcctcg ggcatccaag cagcaagcgc
     2461 gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg cagcagggca
     2521 gtcgccctaa aacaaagtta aacatcatga gggaagcggt gatcgccgaa gtatcgactc
     2581 aactatcaga ggtagttggc gtcatcgagc gccatctcga accgacgttg ctggccgtac
     2641 atttgtacgg ctccgcagtg gatggcggcc tgaagccaca cagcgatatt gatttgctgg
     2701 ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg
     2761 aaacttcggc ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc accattgttg
     2821 tgcacgacga catcattccg tggcgttatc cagctaagcg cgaactgcaa tttggagaat
     2881 ggcagcgcaa tgacattctt gcaggtatct tcgagccagc cacgatcgac attgatctgg
     2941 ctatcttgct gacaaaagca agagaacata gcgttgcctt ggtaggtcca gcggcggagg
     3001 aactctttga tccggttcct gaacaggatc tatttgaggc gctaaatgaa accttaacgc
     3061 tatggaactc gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc
     3121 gcatttggta cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg
     3181 caatggagcg cctgccggcc cagtatcagc ccgtcatact tgaagctaga caggcttatc
     3241 ttggacaaga agaagatcgc ttggcctcgc gcgcagatca gttggaagaa tttgtccatt
     3301 acgtaaaagg cgagatcacc aaggtagtcg gcaaataact gtcagaccaa gtttactcat
     3361 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     3421 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     3481 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     3541 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     3601 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     3661 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     3721 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     3781 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     3841 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     3901 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     3961 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     4021 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     4081 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     4141 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     4201 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcga
//
