LOCUS       sequence-391018-        2872 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobrick (MoClo) Level 0 Part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2872)
  AUTHORS   Chamness JC, Kumar J, Cruz AJ, Rhuby E, Holum MJ, Cody JP, Tibebu R,
            Gamo ME, Starker CG, Zhang F, Voytas DF
  TITLE     An extensible vector toolkit and parts library for advanced 
            engineering of plant genomes.
  JOURNAL   Plant Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312.
  PUBMED    36896468
REFERENCE   2  (bases 1 to 2872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312."
COMMENT     Sequence Label: pMC-1-Ugi3-11
FEATURES             Location/Qualifiers
     source          1..2872
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(752..770)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        838..942
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1164..1955
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2126..2714
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2615..2634
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(2868..2872,1..13)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
       61 gccgattcat taatcactct gtggtctcag gagaaaccag taagttgcaa tacatccatt
      121 gatggtagtc cccatccatg actcaacaca gccttgattg tgtctaaaca atttttttgt
      181 ttccttaatc tatccacatt gattattctt cctgtcattg ttatgctgtg atgctgcctg
      241 atgtcgtgtt cttgcttcct ttttatgatt ctacttgcct tttcctgaac tatacttgtt
      301 cctgattact cacatgcttc acatccgcag atggcatcgt gtgcccgtcc ttgaaaacac
      361 ataaacgttt atgtggcccc ccttgcaggc aacacttctg tccaacacct ttcccaatcc
      421 ttgggaaaca aaatggaaca ccagcccccc aatccccatc ttcaattttt ggtaacatag
      481 atagtttatt taaaatttta gttatatgat ttaaaataaa atataattta cgataaatat
      541 ttttaaccaa attattgaat gggagaaaga ggaaggggtt gttttaggca ttgaattgat
      601 gtaactaaaa tttaaccctg gcaggcagtt gcattgattg cagttttcat ggggagggga
      661 ttacacgata gtacaaaaca tatacaccaa gaattgaata aacttgagaa ttcgcttgag
      721 accacgaagt ggctcttcag tggacgaaag ggcctcgtga tacgcctatt tttataggtt
      781 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
      841 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
      901 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgcgctc acgcaactgg
      961 tccagaacct tgaccgaacg cagcggtggt aacggcgcag tggcggtttt catggcttgt
     1021 tatgactgtt tttttttggg gtacagtcta tgcctcgggc atccaagcag caagcgcgtt
     1081 acgccgtggg tcgatgtttg atgttatgga gcagcaacga tgttacgcag cagggcagtc
     1141 gccctaaaac aaagttaaac atcatgaggg aagcggtgat cgccgaagta tcgactcaac
     1201 tatcagaggt agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt
     1261 tgtacggctc cgcagtggat ggcggcctga agccacacag cgatattgat ttgctggtta
     1321 cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa
     1381 cttcggcttc ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc
     1441 acgacgacat cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc
     1501 agcgcaatga cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta
     1561 tcttgctgac aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac
     1621 tctttgatcc ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat
     1681 ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca
     1741 tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa
     1801 tggagcgcct gccggcccag tatcagcccg tcatacttga agctagacag gcttatcttg
     1861 gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gtccattacg
     1921 taaaaggcga gatcaccaag gtagtcggca aataactgtc agaccaagtt tactcatata
     1981 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     2041 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     2101 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     2161 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     2221 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     2281 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     2341 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     2401 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     2461 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     2521 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     2581 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     2641 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     2701 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     2761 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     2821 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc ga
//
