LOCUS       sequence-391022-        2718 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobrick (MoClo) Level 0 Part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2718)
  AUTHORS   Chamness JC, Kumar J, Cruz AJ, Rhuby E, Holum MJ, Cody JP, Tibebu R,
            Gamo ME, Starker CG, Zhang F, Voytas DF
  TITLE     An extensible vector toolkit and parts library for advanced 
            engineering of plant genomes.
  JOURNAL   Plant Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312.
  PUBMED    36896468
REFERENCE   2  (bases 1 to 2718)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312."
COMMENT     Sequence Label: pMC-1-CmYLCV-4
FEATURES             Location/Qualifiers
     source          1..2718
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(598..616)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        684..788
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1010..1801
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1972..2560
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2461..2480
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(2714..2718,1..13)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
       61 gccgattcat taatcactct gtggtctcag gagtggcaga catactgtcc cacaaatgaa
      121 gatggaatct gtaaaagaaa acgcgtgaaa taatgcgtct gacaaaggtt aggtcggctg
      181 cctttaatca ataccaaagt ggtccctacc acgatggaaa aactgtgcag tcggtttggc
      241 tttttctgac gaacaaataa gattcgtggc cgacaggtgg gggtccacca tgtgaaggca
      301 tcttcagact ccaataatgg agcaatgacg taagggctta cgaaataagt aagggtagtt
      361 tgggaaatgt ccactcaccc gtcagtctat aaatacttag cccctccctc attgttaagg
      421 gagcaaaatc tcagagagat agtcctagag agagaaagag agcaagtagc ctagaagtag
      481 tcaaggcggc gaagtattca ggcacgtggc caggaagaag aaaagccaag acgacgaaaa
      541 caggtaagag ctaagcttta cttgagacca cgaagtggct cttcagtgga cgaaagggcc
      601 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
      661 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
      721 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      781 ggaagagtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg
      841 gcgcagtggc ggttttcatg gcttgttatg actgtttttt tttggggtac agtctatgcc
      901 tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag
      961 caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttaaacatca tgagggaagc
     1021 ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg agcgccatct
     1081 cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg gcctgaagcc
     1141 acacagcgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa caacgcggcg
     1201 agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg agattctccg
     1261 cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa
     1321 gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta tcttcgagcc
     1381 agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac atagcgttgc
     1441 cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg atctatttga
     1501 ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg gcgatgagcg
     1561 aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc
     1621 gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc agcccgtcat
     1681 acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct cgcgcgcaga
     1741 tcagttggaa gaatttgtcc attacgtaaa aggcgagatc accaaggtag tcggcaaata
     1801 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     1861 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     1921 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     1981 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     2041 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     2101 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     2161 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     2221 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     2281 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     2341 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     2401 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     2461 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     2521 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     2581 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     2641 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     2701 aacgaccgag cgcagcga
//
