LOCUS       sequence-391024-        3723 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobrick (MoClo) Level 0 Part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3723)
  AUTHORS   Chamness JC, Kumar J, Cruz AJ, Rhuby E, Holum MJ, Cody JP, Tibebu R,
            Gamo ME, Starker CG, Zhang F, Voytas DF
  TITLE     An extensible vector toolkit and parts library for advanced 
            engineering of plant genomes.
  JOURNAL   Plant Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312.
  PUBMED    36896468
REFERENCE   2  (bases 1 to 3723)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312."
COMMENT     Sequence Label: pMC-1-OsEf1a-4
FEATURES             Location/Qualifiers
     source          1..3723
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1603..1621)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1689..1793
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2015..2806
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2977..3565
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3466..3485
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(3719..3723,1..13)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
       61 gccgattcat taatcactct gtggtctcag gagtataatc ccgtgcgttc acttgatcta
      121 gcgatttagg ggggttccct aacctcatac ttattcctaa ctactccctc cgtttcaggt
      181 tataaaatat tttgactttg ttcaaagtca aaatgcttta agtttaaaag tttgtagaaa
      241 aaaagtagta acattttcaa cacaagacaa atttattatg aaaatatatt caattattga
      301 tatgatgata ctaatttagt attataaata ttactatatt tatctataaa cttagttaac
      361 cttgaaatag tttgacttta accaaagtta aaatgtctta taacctgaaa cgaagtaagt
      421 aataactgtc cgcttaaact cactgaatgg ggccttggat actccacaat ccagatcatt
      481 ttgcccgaca ccttgctctc aaaacaacta tatagaccgg tgcaaagtga ctatttgcaa
      541 ctaaacgccc atctacttgt tgcaaatcta cttctcccaa acatcctcct ctaaaaaaaa
      601 tttaaatata cttatcactt gagcgctgcg aaacctacag agacttgaac gtaacgcaaa
      661 cgtttgaaca ggtcgaccgc aggattaaaa tcgaacggtc aatactgcca cgcaacaata
      721 accaccagac aaagatcgga cggccaggaa ggaaccctag cacccgtggc tgcctatata
      781 agaggcaatc ctctcgccct aaccccctcc tctctcccat ctcatcccag ccgccgccgc
      841 cgcagcctcc tcctctcgcc atctcccgtc ctctccttca aggtcagctt cagatcttct
      901 cacttatcct tgtttgcgcc tgttcatgat cgttcgtagc tgcagatctg gtttggtatc
      961 gcgaggtttg ggtgatttgg tgcttgggca tgttgttttc gttcattttt tattgtgtat
     1021 tcctctcact gctgtgatct tagtttgatg tccgttgacg cgaagataaa cttgatttgt
     1081 ccgcgaatca tgtgcgtatg ttggaaatta tcggtagaat cgaacgataa acttgagtta
     1141 ctgcataatc atctacataa gtggaaaact actggtagaa tcggtagaaa atctgtatct
     1201 agaatccata atttgttgtt aaactactgc tgagtagatg ttgttattgt ccgtgccctg
     1261 ttcagttggg tactgaaatt ctgtatctaa aatccaatcc gtaaatttgt ttctgtaaat
     1321 ttgttattgt gctactgctg agtagatggt gttattgtct gtaccctgtt cggttgtgtc
     1381 gattactgcg cgtctgtgta aatctcatgc tgattttgat ggctattaac gttctgtgga
     1441 tgtccaagtg atctgatctg agatcttgcc tgtgatattt atttggagtt aaaaattata
     1501 cagaaaattg tttctaatct ttgttttatt acgtagaaca gtcagctacc tgcttaatca
     1561 acctacttga gaccacgaag tggctcttca gtggacgaaa gggcctcgtg atacgcctat
     1621 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     1681 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     1741 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgcgct
     1801 cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg taacggcgca gtggcggttt
     1861 tcatggcttg ttatgactgt ttttttttgg ggtacagtct atgcctcggg catccaagca
     1921 gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg atgttacgca
     1981 gcagggcagt cgccctaaaa caaagttaaa catcatgagg gaagcggtga tcgccgaagt
     2041 atcgactcaa ctatcagagg tagttggcgt catcgagcgc catctcgaac cgacgttgct
     2101 ggccgtacat ttgtacggct ccgcagtgga tggcggcctg aagccacaca gcgatattga
     2161 tttgctggtt acggtgaccg taaggcttga tgaaacaacg cggcgagctt tgatcaacga
     2221 ccttttggaa acttcggctt cccctggaga gagcgagatt ctccgcgctg tagaagtcac
     2281 cattgttgtg cacgacgaca tcattccgtg gcgttatcca gctaagcgcg aactgcaatt
     2341 tggagaatgg cagcgcaatg acattcttgc aggtatcttc gagccagcca cgatcgacat
     2401 tgatctggct atcttgctga caaaagcaag agaacatagc gttgccttgg taggtccagc
     2461 ggcggaggaa ctctttgatc cggttcctga acaggatcta tttgaggcgc taaatgaaac
     2521 cttaacgcta tggaactcgc cgcccgactg ggctggcgat gagcgaaatg tagtgcttac
     2581 gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc
     2641 cgactgggca atggagcgcc tgccggccca gtatcagccc gtcatacttg aagctagaca
     2701 ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt
     2761 tgtccattac gtaaaaggcg agatcaccaa ggtagtcggc aaataactgt cagaccaagt
     2821 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     2881 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     2941 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     3001 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     3061 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     3121 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     3181 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     3241 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     3301 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     3361 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     3421 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     3481 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     3541 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     3601 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     3661 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     3721 cga
//
