LOCUS       sequence-391025-        2542 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobrick (MoClo) Level 0 Part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Chamness JC, Kumar J, Cruz AJ, Rhuby E, Holum MJ, Cody JP, Tibebu R,
            Gamo ME, Starker CG, Zhang F, Voytas DF
  TITLE     An extensible vector toolkit and parts library for advanced 
            engineering of plant genomes.
  JOURNAL   Plant Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312.
  PUBMED    36896468
REFERENCE   2  (bases 1 to 2542)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312."
COMMENT     Sequence Label: pMC-1-Nos-6
FEATURES             Location/Qualifiers
     source          1..2542
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        168..347
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        200..383
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     primer_bind     complement(422..440)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        508..612
                     /gene="bla"
                     /label=AmpR promoter
     CDS             834..1625
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1796..2384
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2285..2304
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(2538..2542,1..13)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
       61 gccgattcat taatcactct gtggtctcag gagcggagaa ttaagggagt cacgttatga
      121 cccccgccga tgacgcggga caagccgttt tacgtttgga actgacagaa ccgcaacgtt
      181 gaaggagcca ctcagccgcg ggtttctgga gtttaatgag ctaagcacat acgtcagaaa
      241 ccattattgc gcgttcaaaa gtcgcctaag gtcactatca gctagcaaat atttcttgtc
      301 aaaaatgctc cactgacgtt ccataaattc ccctcggtat ccaattagag tctcatattc
      361 actctcaatc caaataatct gcaatgtgag accacgaagt ggctcttcag tggacgaaag
      421 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
      481 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
      541 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
      601 aaaaggaaga gtatgcgctc acgcaactgg tccagaacct tgaccgaacg cagcggtggt
      661 aacggcgcag tggcggtttt catggcttgt tatgactgtt tttttttggg gtacagtcta
      721 tgcctcgggc atccaagcag caagcgcgtt acgccgtggg tcgatgtttg atgttatgga
      781 gcagcaacga tgttacgcag cagggcagtc gccctaaaac aaagttaaac atcatgaggg
      841 aagcggtgat cgccgaagta tcgactcaac tatcagaggt agttggcgtc atcgagcgcc
      901 atctcgaacc gacgttgctg gccgtacatt tgtacggctc cgcagtggat ggcggcctga
      961 agccacacag cgatattgat ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc
     1021 ggcgagcttt gatcaacgac cttttggaaa cttcggcttc ccctggagag agcgagattc
     1081 tccgcgctgt agaagtcacc attgttgtgc acgacgacat cattccgtgg cgttatccag
     1141 ctaagcgcga actgcaattt ggagaatggc agcgcaatga cattcttgca ggtatcttcg
     1201 agccagccac gatcgacatt gatctggcta tcttgctgac aaaagcaaga gaacatagcg
     1261 ttgccttggt aggtccagcg gcggaggaac tctttgatcc ggttcctgaa caggatctat
     1321 ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg gctggcgatg
     1381 agcgaaatgt agtgcttacg ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg
     1441 cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct gccggcccag tatcagcccg
     1501 tcatacttga agctagacag gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg
     1561 cagatcagtt ggaagaattt gtccattacg taaaaggcga gatcaccaag gtagtcggca
     1621 aataactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     1681 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     1741 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     1801 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     1861 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     1921 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     1981 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     2041 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     2101 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     2161 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     2221 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     2281 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     2341 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     2401 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     2461 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     2521 gccgaacgac cgagcgcagc ga
//
