LOCUS       sequence-391026-        3592 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobrick (MoClo) Level 0 Part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3592)
  AUTHORS   Chamness JC, Kumar J, Cruz AJ, Rhuby E, Holum MJ, Cody JP, Tibebu R,
            Gamo ME, Starker CG, Zhang F, Voytas DF
  TITLE     An extensible vector toolkit and parts library for advanced 
            engineering of plant genomes.
  JOURNAL   Plant Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312.
  PUBMED    36896468
REFERENCE   2  (bases 1 to 3592)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312."
COMMENT     Sequence Label: pMC-1-35S_Long-6
FEATURES             Location/Qualifiers
     source          1..3592
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1083..1428
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1371..1391
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     primer_bind     complement(1472..1490)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1558..1662
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1884..2675
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2846..3434
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3335..3354
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(3588..3592,1..13)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
       61 gccgattcat taatcactct gtggtctcag gaggaattcc aatcccacaa aaatctgagc
      121 ttaacagcac agttgctcct ctcagagcag aatcgggtat tcaacaccct catatcaact
      181 actacgttgt gtataacggt ccacatgccg gtatatacga tgactggggt tgtacaaagg
      241 cggcaacaaa cggcgttccc ggagttgcac acaagaaatt tgccactatt acagaggcaa
      301 gagcagcagc tgacgcgtac acaacaagtc agcaaacaga caggttgaac ttcatcccca
      361 aaggagaagc tcaactcaag cccaagagct ttgctaaggc cctaacaagc ccaccaaagc
      421 aaaaagccca ctggctcacg ctaggaacca aaaggcccag cagtgatcca gccccaaaag
      481 agatctcctt tgccccggag attacaatgg acgatttcct ctatctttac gatctaggaa
      541 ggaagttcga aggtgaaggt gacgacacta tgttcaccac tgataatgag aaggttagcc
      601 tcttcaattt cagaaagaat gctgacccac agatggttag agaggcctac gcagcaagtc
      661 tcatcaagac gatctacccg agtaacaatc tccaggagat caaatacctt cccaagaagg
      721 ttaaagatgc agtcaaaaga ttcaggacta attgcatcaa gaacacagag aaagacatat
      781 ttctcaagat cagaagtact attccagtat ggacgattca aggcttgctt cataaaccaa
      841 ggcaagtaat agagattgga gtctctaaaa aggtagttcc tactgaatct aaggccatgc
      901 atggagtcta agattcaaat cgaggatcta acagaactcg ccgtcaagac tggcgaacag
      961 ttcatacaga gtcttttacg actcaatgac aagaagaaaa tcttcgtcaa catggtggag
     1021 cacgacactc tggtctactc caaaaatgtc aaagatacag tctcagaaga tcaaagggct
     1081 attgagactt ttcaacaaag gataatttcg ggaaacctcc tcggattcca ttgcccagct
     1141 atctgtcact tcatcgaaag gacagtagaa aaggaaggtg gctcctacaa atgccatcat
     1201 tgcgataaag gaaaggctat cattcaagat ctctctgccg acagtggtcc caaagatgga
     1261 cccccaccca cgaggagcat cgtggaaaaa gaagaggttc caaccacgtc tacaaagcaa
     1321 gtggattgat gtgacatctc cactgacgta agggatgacg cacaatccca ctatccttcg
     1381 caagaccctt cctctatata aggaagttca tttcatttgg agaggacacg ctaatgtgag
     1441 accacgaagt ggctcttcag tggacgaaag ggcctcgtga tacgcctatt tttataggtt
     1501 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
     1561 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     1621 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgcgctc acgcaactgg
     1681 tccagaacct tgaccgaacg cagcggtggt aacggcgcag tggcggtttt catggcttgt
     1741 tatgactgtt tttttttggg gtacagtcta tgcctcgggc atccaagcag caagcgcgtt
     1801 acgccgtggg tcgatgtttg atgttatgga gcagcaacga tgttacgcag cagggcagtc
     1861 gccctaaaac aaagttaaac atcatgaggg aagcggtgat cgccgaagta tcgactcaac
     1921 tatcagaggt agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt
     1981 tgtacggctc cgcagtggat ggcggcctga agccacacag cgatattgat ttgctggtta
     2041 cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa
     2101 cttcggcttc ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc
     2161 acgacgacat cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc
     2221 agcgcaatga cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta
     2281 tcttgctgac aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac
     2341 tctttgatcc ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat
     2401 ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca
     2461 tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa
     2521 tggagcgcct gccggcccag tatcagcccg tcatacttga agctagacag gcttatcttg
     2581 gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gtccattacg
     2641 taaaaggcga gatcaccaag gtagtcggca aataactgtc agaccaagtt tactcatata
     2701 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     2761 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     2821 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     2881 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     2941 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     3001 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     3061 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     3121 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     3181 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     3241 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     3301 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     3361 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     3421 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     3481 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     3541 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc ga
//
