LOCUS       sequence-391030-        2723 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobrick (MoClo) Level 0 Part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2723)
  AUTHORS   Chamness JC, Kumar J, Cruz AJ, Rhuby E, Holum MJ, Cody JP, Tibebu R,
            Gamo ME, Starker CG, Zhang F, Voytas DF
  TITLE     An extensible vector toolkit and parts library for advanced 
            engineering of plant genomes.
  JOURNAL   Plant Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312.
  PUBMED    36896468
REFERENCE   2  (bases 1 to 2723)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312."
COMMENT     Sequence Label: pMC-1-GmHSP17-5E-6
FEATURES             Location/Qualifiers
     source          1..2723
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(603..621)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        689..793
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1015..1806
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1977..2565
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2466..2485
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(2719..2723,1..13)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
       61 gccgattcat taatcactct gtggtctcag gagtttccac ttcaattcct cctctatggt
      121 ttcagtgttc tgcttgaaat tttttagata tttttcttaa catttcaaaa agtatttttt
      181 gaaaaacaaa acaatattct aaaaaatact ttccgtattt atttattctg gacagtaaaa
      241 taaaataata tttcagaaag tgcattacga aaataacgac tttgaaacat tgttataggt
      301 ataaagaatt tctatatgat gatgaaaaat agaaaaacct actaatgtat ttatgaatga
      361 tgtccagaag tggaagaaaa ataaatataa tgatgtgtag taaacaagaa ccttcgtaca
      421 tggtgtggag aattccagac gtttttatgg aatcatctga aactggtgaa ttcaaccaaa
      481 ttgcaaaaag taggattttt ctggaacata caagattatc ctttcacttc ctttaaatac
      541 ctcgcgtatc cccttcgtcc tcgaatgtga gaccacgaag tggctcttca gtggacgaaa
      601 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
      661 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
      721 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
      781 aaaaaggaag agtatgcgct cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg
      841 taacggcgca gtggcggttt tcatggcttg ttatgactgt ttttttttgg ggtacagtct
      901 atgcctcggg catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg
      961 agcagcaacg atgttacgca gcagggcagt cgccctaaaa caaagttaaa catcatgagg
     1021 gaagcggtga tcgccgaagt atcgactcaa ctatcagagg tagttggcgt catcgagcgc
     1081 catctcgaac cgacgttgct ggccgtacat ttgtacggct ccgcagtgga tggcggcctg
     1141 aagccacaca gcgatattga tttgctggtt acggtgaccg taaggcttga tgaaacaacg
     1201 cggcgagctt tgatcaacga ccttttggaa acttcggctt cccctggaga gagcgagatt
     1261 ctccgcgctg tagaagtcac cattgttgtg cacgacgaca tcattccgtg gcgttatcca
     1321 gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg acattcttgc aggtatcttc
     1381 gagccagcca cgatcgacat tgatctggct atcttgctga caaaagcaag agaacatagc
     1441 gttgccttgg taggtccagc ggcggaggaa ctctttgatc cggttcctga acaggatcta
     1501 tttgaggcgc taaatgaaac cttaacgcta tggaactcgc cgcccgactg ggctggcgat
     1561 gagcgaaatg tagtgcttac gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc
     1621 gcgccgaagg atgtcgctgc cgactgggca atggagcgcc tgccggccca gtatcagccc
     1681 gtcatacttg aagctagaca ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc
     1741 gcagatcagt tggaagaatt tgtccattac gtaaaaggcg agatcaccaa ggtagtcggc
     1801 aaataactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     1861 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     1921 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     1981 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     2041 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     2101 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     2161 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     2221 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     2281 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     2341 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     2401 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     2461 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     2521 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     2581 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     2641 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     2701 agccgaacga ccgagcgcag cga
//
