LOCUS       sequence-391033-        3781 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobrick (MoClo) Level 0 Part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3781)
  AUTHORS   Chamness JC, Kumar J, Cruz AJ, Rhuby E, Holum MJ, Cody JP, Tibebu R,
            Gamo ME, Starker CG, Zhang F, Voytas DF
  TITLE     An extensible vector toolkit and parts library for advanced 
            engineering of plant genomes.
  JOURNAL   Plant Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312.
  PUBMED    36896468
REFERENCE   2  (bases 1 to 3781)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312."
COMMENT     Sequence Label: pMC-1-OsUbi-6
FEATURES             Location/Qualifiers
     source          1..3781
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1661..1679)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1747..1851
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2073..2864
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3035..3623
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3524..3543
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(3777..3781,1..13)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
       61 gccgattcat taatcactct gtggtctcag gagtctctgc acaaagtgca tcctgggctg
      121 cttcaattat aaagccccat tcaccacatt tgctagatag tcgaaaagca ccatcaatat
      181 tgagcttcag gtatttttgg ttgtgttgtg gttggattga ttctaatata taccaaatca
      241 atataattca ctaccaaaat ataccatagc catcacaact ttattaattt tggtagctta
      301 agatggtata tataataacc aattaacaac tgattctaat tttactacgg cccagtatct
      361 accaatacaa aacaacgagt atgttttctt ccatcgtaat cgtacacagt acaaaaaaac
      421 ctggccagcc tttattgggc tggggctctc tttcgaaagg tcacaaaacg tacacggcag
      481 taacgccctt cgctgcgtgt taacggccac caaccccgcc gtgagcaaac ggcatcagct
      541 ttccacctcc tcgatatctc cgcggcgccg tctggacccg ccccctttcc gttcctttct
      601 ttccttctcg cgtttgcgtg gtggggacgg actccccaaa ccgcctctcc ctctctttat
      661 ttgtctatat tctcactggg ccccacccac cgcacccctg ggcccactca cgagtccccc
      721 cctcccgacc tataaatacc ccaccccctc ctcgcctctt cctccatcaa tcgaatcccc
      781 aaaatcgcag agaaaaaaaa atctcccctc gaagcgaagc gtcgaatcgc cttctcaagg
      841 tatgcgattt tctgatcctc tccgttcctc gcgttcgatt tgatttcccg gcctgtccgt
      901 gattgtgaga tgttgtggtt agtctccgtt ttgcgatctg ttgtagattt gaacaggttt
      961 agatggggtt cgcgtggtat gctggatctg tgattatgag cgatgctgtt cgtggtccaa
     1021 gtattgattg gttcggatct agaagtagaa gtagaactgt gctagggttg tgatttgttc
     1081 cgatctgttc aatcagtagg atttagtctc tgtttttctc gttgatccaa gtagcagctt
     1141 caggtatatt ttgcttaggc tgtttttgat tcagtccctc tagttgcata gattctactc
     1201 tgttcatgtt taatctaagg gctgcgtctt gttgattagt gattacatag catagctgtc
     1261 aggatatttt acttgcttat gcctatctta tcaactgttg cacctgtaaa ttctagccta
     1321 tgttaattaa cctgccttat gtgctctcgg gatagtgcta gtagttattg aatcagtttg
     1381 ccgatggaat tctagtagtt catagacctg cagattattt ttgtgaactc gagcacggtg
     1441 cgtgtctcta ttttgttagg tcactgttgg tgttgatagg tacactgatg ttattgtggt
     1501 ttaggtcgtg tatctaacat attggaataa tttgattgac tgatttctgc tgtacttgct
     1561 tggtattgtt ataatttcat gttcatagtt gctgaccatg cttcggtaat tgtgtgtgca
     1621 gaatgtgaga ccacgaagtg gctcttcagt ggacgaaagg gcctcgtgat acgcctattt
     1681 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
     1741 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     1801 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgcgctca
     1861 cgcaactggt ccagaacctt gaccgaacgc agcggtggta acggcgcagt ggcggttttc
     1921 atggcttgtt atgactgttt ttttttgggg tacagtctat gcctcgggca tccaagcagc
     1981 aagcgcgtta cgccgtgggt cgatgtttga tgttatggag cagcaacgat gttacgcagc
     2041 agggcagtcg ccctaaaaca aagttaaaca tcatgaggga agcggtgatc gccgaagtat
     2101 cgactcaact atcagaggta gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg
     2161 ccgtacattt gtacggctcc gcagtggatg gcggcctgaa gccacacagc gatattgatt
     2221 tgctggttac ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg atcaacgacc
     2281 ttttggaaac ttcggcttcc cctggagaga gcgagattct ccgcgctgta gaagtcacca
     2341 ttgttgtgca cgacgacatc attccgtggc gttatccagc taagcgcgaa ctgcaatttg
     2401 gagaatggca gcgcaatgac attcttgcag gtatcttcga gccagccacg atcgacattg
     2461 atctggctat cttgctgaca aaagcaagag aacatagcgt tgccttggta ggtccagcgg
     2521 cggaggaact ctttgatccg gttcctgaac aggatctatt tgaggcgcta aatgaaacct
     2581 taacgctatg gaactcgccg cccgactggg ctggcgatga gcgaaatgta gtgcttacgt
     2641 tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg
     2701 actgggcaat ggagcgcctg ccggcccagt atcagcccgt catacttgaa gctagacagg
     2761 cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg
     2821 tccattacgt aaaaggcgag atcaccaagg tagtcggcaa ataactgtca gaccaagttt
     2881 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     2941 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     3001 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     3061 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     3121 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     3181 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     3241 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     3301 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     3361 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     3421 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     3481 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     3541 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     3601 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     3661 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     3721 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     3781 a
//
