LOCUS       sequence-391034-        4153 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobrick (MoClo) Level 0 Part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4153)
  AUTHORS   Chamness JC, Kumar J, Cruz AJ, Rhuby E, Holum MJ, Cody JP, Tibebu R,
            Gamo ME, Starker CG, Zhang F, Voytas DF
  TITLE     An extensible vector toolkit and parts library for advanced 
            engineering of plant genomes.
  JOURNAL   Plant Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312.
  PUBMED    36896468
REFERENCE   2  (bases 1 to 4153)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312."
COMMENT     Sequence Label: pMC-1-PvUbi2-6
FEATURES             Location/Qualifiers
     source          1..4153
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(2033..2051)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2119..2223
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2445..3236
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3407..3995
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3896..3915
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(4149..4153,1..13)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
       61 gccgattcat taatcactct gtggtctcag gagcctgcaa cgccggcgta atatggcgcg
      121 ccgaagccaa ctaaacaaga ccataaccat ggtgacattt gacatagttg tttactactt
      181 gcttgagccc cacccttgct tatcggttga acattacaag atacactgcg ggtggcctaa
      241 ggcacaccgt ccgaaaccgg caaaccaagc ctgatcgccg aaatccaaaa tcactaccgg
      301 caatctctaa agtttatttc atccttatat gacgaggaaa gaaaagaaga gagaaataat
      361 atcttaactt ctaaatcagt cgcgtcaact ttctcggcta agaaagtgag cactatcatt
      421 tcgaagacca tgtcatgagt gccgacttgc catatcttat tatattctta tttatttaat
      481 tataatccca ttgcaatacg tctattctat catggcctgc cactaacgct ccgtctaacg
      541 tcgttaagcc attgtcataa gcggctgctc aaaactcttc ccggtggagg cgaggcgtta
      601 acggcgtcta caaatctaac ggccaccaac catccagccg cctctcgaaa gctccgctcc
      661 gatcgcggaa attgcgtggc ggagacgagc gggctcctct cacacggccc ggaaccgtca
      721 cggcacgggt gggggattcc ttccccaacc ctccccacct ctcctccccc cgtcgcagcc
      781 cataaataca gggccctccg cgcctcttcc cacaatctca catcgtctca tcgttcggag
      841 cgcacaaccc ccgggttcca aatccaaatt gctctcctcg cgaccctcgg cgatccttcc
      901 cccgcttcaa ggtacggcga tcgtctcccc cgtcctcttg ccccatctcc tcgctcggcg
      961 tggtttggtg gttctgcttg gtctgtggct aggaactagg ctgaggcgtt gacgaaatca
     1021 tgctagatcc gcgtgtttcc tgatcgtggg tggctgggag gtggggtttt cgtgtagatc
     1081 tgatcggttc cgctgtttat cctgtcatgc tcatgtgatt tgtggggatt ttaggtcgtt
     1141 tgtccgggaa tcgtggggtt gcttctaggc tgttcgtaga tgagatcgtt ctcacgatct
     1201 gctgggtcgc tgcctaggtt cagctaggtc tgccctgttt ttgggttcgt tttcgggatc
     1261 tgtacgtgca tctattatct ggttcgatgg tgctagctag gaacaaacaa ctgattcgtc
     1321 cgatcgattg ttttgttgcc atgtgcaagg ttaggtcgtt atctgattgc tgtagatcag
     1381 agtagaataa gatcatcaca agctagctct tgggcttatt atgaatctgc gtttgttgca
     1441 tgattaagat gattatgctt tttcttatgc tgccgtttgt atatgatgcg gtagctttta
     1501 actgaatagc acacctttcc tgtttagtta gattagatta gattgcatga tagatgagga
     1561 tatatgctgc tacatcagtt tgatgattct ctggtacctc ataatcaact agctcatgtg
     1621 cttaaattga aactgcatgt gccacatgat taagatgcta agattggtga agatatatac
     1681 gctgctgttc ctataggatc ctgtagcttt tacctggtca acatgcatcg tcctgttatg
     1741 gatagatatg catgatagat gaagatatgt actgctacaa tttgatgatt cttttgtgca
     1801 cctgatgatc atgcatgctc tttgccctta ctttgatata cttggatgat ggcatgctta
     1861 gtactaatga tgtgatgaac acacatgacc tgttggtatg aatatgatgt tgctgtttgc
     1921 ttgtgatgag ttctgtttgt ttactgctag gcacttaccc tgttgtctgg ttctcttttg
     1981 cagaacctgc aggaatgtga gaccacgaag tggctcttca gtggacgaaa gggcctcgtg
     2041 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
     2101 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     2161 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     2221 agtatgcgct cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg taacggcgca
     2281 gtggcggttt tcatggcttg ttatgactgt ttttttttgg ggtacagtct atgcctcggg
     2341 catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg
     2401 atgttacgca gcagggcagt cgccctaaaa caaagttaaa catcatgagg gaagcggtga
     2461 tcgccgaagt atcgactcaa ctatcagagg tagttggcgt catcgagcgc catctcgaac
     2521 cgacgttgct ggccgtacat ttgtacggct ccgcagtgga tggcggcctg aagccacaca
     2581 gcgatattga tttgctggtt acggtgaccg taaggcttga tgaaacaacg cggcgagctt
     2641 tgatcaacga ccttttggaa acttcggctt cccctggaga gagcgagatt ctccgcgctg
     2701 tagaagtcac cattgttgtg cacgacgaca tcattccgtg gcgttatcca gctaagcgcg
     2761 aactgcaatt tggagaatgg cagcgcaatg acattcttgc aggtatcttc gagccagcca
     2821 cgatcgacat tgatctggct atcttgctga caaaagcaag agaacatagc gttgccttgg
     2881 taggtccagc ggcggaggaa ctctttgatc cggttcctga acaggatcta tttgaggcgc
     2941 taaatgaaac cttaacgcta tggaactcgc cgcccgactg ggctggcgat gagcgaaatg
     3001 tagtgcttac gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg
     3061 atgtcgctgc cgactgggca atggagcgcc tgccggccca gtatcagccc gtcatacttg
     3121 aagctagaca ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt
     3181 tggaagaatt tgtccattac gtaaaaggcg agatcaccaa ggtagtcggc aaataactgt
     3241 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     3301 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     3361 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     3421 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     3481 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     3541 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     3601 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     3661 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     3721 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     3781 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     3841 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     3901 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     3961 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     4021 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     4081 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     4141 ccgagcgcag cga
//
