LOCUS       sequence-391041-        9444 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  In situ cell-surface proteome extraction by extracellular labeling 
            (iPEEL).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9444)
  AUTHORS   Shuster SA, Li J, Chon U, Sinantha-Hu MC, Luginbuhl DJ, Udeshi ND, 
            Carey DK, Takeo YH, Xie Q, Xu C, Mani DR, Han S, Ting AY, Carr SA, 
            Luo L
  TITLE     In situ cell-type-specific cell-surface proteomic profiling in mice.
  JOURNAL   Neuron. 2022 Dec 7;110(23):3882-3896.e9. doi: 
            10.1016/j.neuron.2022.09.025. Epub 2022 Oct 10.
  PUBMED    36220098
REFERENCE   2  (bases 1 to 9444)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2022.09.025"; journalName: "Neuron"; date: 
            "2022-12-7- 7"; volume: "110"; issue: "23"; pages: "3882-3896.e9"
COMMENT     Sequence Label: pCAG-FRT-stop-FRT(FSF)-
            loxP-stop-loxP(LSL)-SP...
FEATURES             Location/Qualifiers
     source          1..9444
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(624..1415,1416..1484))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1247..1266
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1485..1589)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1615..2070)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1752..1773)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1964..1983
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2197..2219
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2211..2228
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2212..2228
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2235..2254
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2235..2253
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    2304..2373
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     enhancer        2679..2982
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2984..3261
                     /label=chicken beta-actin promoter
     intron          3262..4278
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     4202..4224
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     4286..4305
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    4363..4396
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     polyA_signal    4442..4490
                     /note="synthetic polyadenylation signal"
     polyA_signal    4499..4975
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(4698..4717)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(5010..5029)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5054..5102
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     protein_bind    5262..5295
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    5310..5343
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    5510..5644
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    5764..5898
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    6018..6152
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    6181..6214
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      6302..6311
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6371..6397
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             7349..7378
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     misc_feature    7718..8306
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7771..7791)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8189..8200)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8309..8325)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8327..8344)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8333..8540
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    8584..8653
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     promoter        complement(8868..8886)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8907..8923)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8907..8923)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8920..8942)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8931..8947
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8955..8961,8962..8979,8980..8985))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9000..9021
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9138..9155)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(9309..9444,1..453))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9389..9408)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
       61 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
      121 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
      181 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
      241 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
      301 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
      361 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
      421 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
      481 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
      541 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
      601 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
      661 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
      721 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
      781 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
      841 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
      901 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
      961 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     1021 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     1081 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     1141 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     1201 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     1261 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     1321 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     1381 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     1441 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     1501 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     1561 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctaaattg
     1621 taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta
     1681 accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt
     1741 tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca
     1801 aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa
     1861 gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat
     1921 ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag
     1981 gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg
     2041 ccgcgcttaa tgcgccgcta cagggcgcgt cccattcgcc attcaggctg cgcaactgtt
     2101 gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg
     2161 ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga
     2221 cggccagtga attgtaatac gactcactat agggcgaatt gggtaccggg ccccccctcg
     2281 aggtcgacga tgtaggtcac ggtctcgaag ccgcggtgcg ggtgccaggg cgtgcccttg
     2341 ggctccccgg gcgcgtactc cacctcaccc atctggtcca tcatgatgaa cgggtcgagg
     2401 tggcggtagt tgatcccggc gaacgcgcgg cgcaccggga agccctcgcc ctcgaaaccg
     2461 ctgggcgcgg tggtcacggt gagcacggga cgtgcgacgg cgtcggcggg tgcggatacg
     2521 cggggcagcg tcagcgggtt ctcgacggtc acggcgggca tgtcgacggt atcgataagc
     2581 ttcccttaat taaccgttta aacaattctg caggaatcta gttattaata gtaatcaatt
     2641 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
     2701 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
     2761 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
     2821 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
     2881 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
     2941 acttggcagt acatctacgt attagtcatc gctattacca tggtcgaggt gagccccacg
     3001 ttctgcttca ctctccccat ctcccccccc tccccacccc caattttgta tttatttatt
     3061 ttttaattat tttgtgcagc gatgggggcg gggggggggg gggggcgcgc gccrggsggg
     3121 gcggggcggg gsgrggggsg gggcggggcg aggcggagag gtgcggcggc agccaatcag
     3181 agcggcgcgc tccgaaagtt tccttttatg gcgaggcggc ggcggcggcg gccctataaa
     3241 aagcgaagcg cgcggcgggc gggagtcgct gcgcgctgcc ttcgccccgt gccccgctcc
     3301 gccgccgcct cgcgccgccc gccccggctc tgactgaccg cgttactccc acaggtgagc
     3361 gggcgggacg gcccttctcc tccgggctgt aattagcgct tggtttaatg acggcttgtt
     3421 tcttttctgt ggctgcgtga aagccttgag gggctccggg agggcccttt gtgcgggggg
     3481 agcggctcgg ggggtgcgtg cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg
     3541 ctgcccggcg gctgtgagcg ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg
     3601 cgcgagggga gcgcggccgg gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac
     3661 aaaggctgcg tgcggggtgt gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc
     3721 gggctgcaac cccccctgca cccccctccc cgagttgctg agcacggccc ggcttcgggt
     3781 gcggggctcc gtacggggcg tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag
     3841 gtgggggtgc cgggcggggc ggggccgcct cgggccgggg rgggctcggg ggrggggcgc
     3901 ggcggccccc ggagcgccgg cggctgtcga ggcgcggcga gccgcagcca ttgcctttta
     3961 tggtaatcgt gcgagagggc gcagggactt cctttgtccc aaatctgtgc ggagccgaaa
     4021 tctgggaggc gccgccgcac cccctctagc gggcgcgggg cgaagcggtg cggcgccggc
     4081 aggaaggaaa tgggcgggga gggccttcgt gcgtcgccgc gccgccgtcc ccttctccct
     4141 ctccagcctc ggggctgtcc gcggggggac ggctgccttc gggggggacg gggcagggcg
     4201 gggttcggct tctggcgtgt gaccggcggc tctagagcct ctgctaacca tgttcatgcc
     4261 ttcttctttt tcctacagct cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt
     4321 ggcaaagaat tgatttgata ccgcgggccc gggctattcg aagaagttcc tattctctag
     4381 aaagtatagg aacttccctc agcaccatgg ctagcggcag cctcggagtt tgaatagata
     4441 gaataaaata tctttatttt cattccatct gtgtgttggt tttttgtgtg agatctacgg
     4501 gtggcatccc tgtgacccct ccccagtgcc tctcctggcc ctggaagttg ccactccagt
     4561 gcccaccagc cttgtcctaa taaaattaag ttgcatcatt ttgtctgact aggtgtcctt
     4621 ctataatatt atggggtgga ggggggtggt atggagcaag gggcaagttg ggaagacaac
     4681 ctgtagggcc tgcggggtct attgggaacc aagctggagt gcagtggcac aatcttggct
     4741 cactgcaatc tccgcctcct gggttcaagc gattctcatg cctcaggctc ccgagttgtt
     4801 gggattccag gcatgcatga ccaggctcag ctaatttttg tttttttggt agagacgggg
     4861 tttcaccata ttggccaggc tggtctccaa ctcctaatct caggtgatct acccaccttg
     4921 gcctcccaaa ttgctgggat tacaggcgtg aaccactgct cccttccctg tccttctgcc
     4981 tcagctaatt gagtaggggg gaggctaact gaaacacgga aggagacaat accggaagga
     5041 acccgcgcta tgacggcaat aaaaagacag aataaaacgc acgggtgttg ggtcgtttgt
     5101 tcataaacgc ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca
     5161 ttggggccaa tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga
     5221 aggcccaggg ctcgcagcca acgtcggggc ggcaggccgt ggaagttcct attctctaga
     5281 aagtatagga acttcctcga gatttaaata taacttcgta taatgtatgc tatacgaagt
     5341 tattcgcgat gaataaatga aagcttgcag atctgcgact ctagaggatc tgcgactcta
     5401 gaggatcata atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc
     5461 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat
     5521 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     5581 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     5641 gatctgcgac tctagaggat cataatcagc cataccacat ttgtagaggt tttacttgct
     5701 ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt
     5761 gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     5821 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     5881 tcttatcatg tctggatctg cgactctaga ggatcataat cagccatacc acatttgtag
     5941 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga
     6001 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     6061 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     6121 aactcatcaa tgtatcttat catgtctgga tccccatcaa gctgatccgg aacccttaat
     6181 ataacttcgt ataatgtatg ctatacgaag ttattaggtc cctcgacctg cagcccaagc
     6241 tagatcgaat tcggccggcc ttcacgtgtt gcacttaacg cgttaagtgc aacacgatcc
     6301 cgccaccatg gagactgata cattgctgct gtgggtactt ctcctctggg tccccggctc
     6361 tacaggcgac tatccttacg atgtcccaga ttatgctgga gctcaacagc tgaccccaac
     6421 cttctacgac aattcatgtc caaacgtctc caacattgtt agggacacta tagttaacga
     6481 actgagatca gatcctcgca ttgccgcttc aattctgcga ctgcacttcc atgattgttt
     6541 cgttaatggc tgcgacgctt caatcctgct tgacaatact acctcattta ggacagagaa
     6601 ggacgctttt ggaaatgcca attctgcaag aggcttccca gtgatagaca gaatgaaggc
     6661 agcagtcgag agtgcttgcc ctcgcaccgt tagctgtgct gaccttctga ccattgctgc
     6721 tcagcagtct gtaactctcg caggtggccc ctcatggagg gtccccctgg gccgaagaga
     6781 ttcacttcag gccttcctcg atttggccaa tgctaacctg ccagcaccct ttttcactct
     6841 gcctcagctc aaagattctt tcagaaatgt aggtctcaat cgatctagcg acctcgtagc
     6901 cttgtcagga ggacacacat tcggaaaaaa ccagtgtcga tttattatgg acaggctcta
     6961 taacttcagc aatactggcc ttcctgaccc caccttgaac acaacatacc ttcaaactct
     7021 cagaggtctc tgccccctta acggtaacct tagtgcactg gtggacttcg atcttcggac
     7081 ccccaccatc ttcgacaata aatactacgt gaatctcgaa gaacaaaagg gtcttataca
     7141 aagtgaccaa gagcttttct cttcccccaa cgctactgac acaataccat tggtgagatc
     7201 attcgccaac tccacccaga ccttctttaa tgcttttgtg gaagctatgg accgcatggg
     7261 taacatcact cctttgactg gcacccaagg gcaaatacgc ctgaactgcc gcgtcgtgaa
     7321 tagcaactct ccacgcttgc aagttgacga acagaaactt atcagtgagg aggaccttaa
     7381 tgccgtgggc caagacacac aggaagtcat agttgtacca catagtcttc cattcaaggt
     7441 agtagtcata tccgccattc ttgctctcgt cgtattgaca attattagcc tgattattct
     7501 gatcatgctc tggcaaaaaa agccacgcgg ttctggaaaa tcacgcataa cctcagaagg
     7561 ggaatatatt ccactggacc agatcgatat taatgtgggg tccgggtttt gttatgagaa
     7621 tgaggtgggg tctggataag aattgtgttg cacttaacgc gttaagtgca acacgtgaag
     7681 gccggccctg caggaattcg atatcaagct tatcgataat caacctctgg attacaaaat
     7741 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     7801 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     7861 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     7921 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     7981 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     8041 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     8101 gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct
     8161 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     8221 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
     8281 gatctccctt tgggccgcct ccccgcatcg ataccgtcga cctcgacctc gactgtgcct
     8341 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
     8401 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
     8461 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac
     8521 aatggcaggc atgctgggga actagttcta gagcggccgc cactcgacga tgtaggtcac
     8581 ggtctcgaag ccgcggtgcg ggtgccaggg cgtgcccttg ggctccccgg gcgcgtactc
     8641 cacctcaccc atctggtcca tcatgatgaa cgggtcgagg tggcggtagt tgatcccggc
     8701 gaacgcgcgg cgcaccggga agccctcgcc ctcgaaaccg ctgggcgcgg tggtcacggt
     8761 gagcacggga cgtgcgacgg cgtcggcggg tgcggatacg cggggcagcg tcagcgggtt
     8821 ctcgacggtc acggcgggca tgtcgagtgg agctccagct tttgttccct ttagtgaggg
     8881 ttaatttcga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     8941 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     9001 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     9061 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     9121 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     9181 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     9241 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     9301 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     9361 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     9421 ctcgtgcgct ctcctgttcc gacc
//
