LOCUS       sequence-391050-       15762 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses FLAG-tagged mutated SMG1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15762)
  AUTHORS   McCann JJ, Fleenor DE, Chen J, Lai CH, Bass TE, Kastan MB
  TITLE     Participation of ATM, SMG1, and DDX5 in a DNA Damage-Induced 
            Alternative Splicing Pathway.
  JOURNAL   Radiat Res. 2023 Apr 1;199(4):406-421. doi: 10.1667/RADE-22-00219.1.
  PUBMED    36921295
REFERENCE   2  (bases 1 to 15762)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1667/RADE-22-00219"; journalName: "Radiat Res"; date: 
            "2023-04-1- 1"; volume: "199"; issue: "4"; pages: "406-421"
COMMENT     Sequence Label: FLAG SMG1 T3550A/S3556A
FEATURES             Location/Qualifiers
     source          1..15762
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        83..462
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        463..666
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     617..637
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             700..723
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    6088..6099
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     polyA_signal    11868..12490
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(12067..12086)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(12514..12534)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        12519..12848
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      12699..12834
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(12886..12905)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(12887..12905)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(12919..12935)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12919..12935)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12932..12954)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12943..12959
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12967..12973,12974..12991,12992..12997))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13012..13033
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(13150..13167)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13321..13909)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13401..13420)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(14080..14871,14872..14940))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14703..14722
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14941..15045)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      15072..15527
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(15159..15178)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     15369..15390
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     15653..15675
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     15667..15684
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     15668..15684
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ggctattggc cattgcatac gttgtatcta tatcataata tgtacattta tattggctca
       61 tgtccaatat gaccgccatg ttgacattga ttattgacta gttattaata gtaatcaatt
      121 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
      181 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
      241 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
      301 actgcccact tggcagtaca tcaagtgtat catatgccaa gtccgccccc tattgacgtc
      361 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttacg ggactttcct
      421 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
      481 tacaccaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
      541 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaat
      601 aaccccgccc cgttgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
      661 agagctcgtt tagtgaaccg tcagaattga tctaccatgg actacaaaga cgatgacgac
      721 aagcttgcgg ccgcgaattc atcgatagat ctgatatcgg taccagtcga gatccatgtc
      781 gtgtcgggga aggtaatgag ccgcagagcc ccggggtctc ggctgagcag cggcggcggc
      841 ggcggcggca ccaagtatcc gcggagctgg aatgactggc aacccagaac tgatagtgca
      901 tcagccgacc cagataattt aaaatattct tcatccagag atagaggtgg ttcttcctct
      961 tacggactgc aaccttcaaa ttcagctgtg gtgtctcggc aaaggcacga tgataccaga
     1021 gtccacgctg acatacagaa tgacgaaaag ggtggctaca gtgtcaatgg aggatctggg
     1081 gaaaatactt atggtcggaa gtcgttgggg caagagctga gggttaacaa tgtgaccagc
     1141 cctgagttca ccagtgttca gcatggcagt cgtgctttag ccaccaaaga catgaggaaa
     1201 tcacaggaga gatcgatgtc ttattctgat gagtctcgac tgtcgaatct tcttcggagg
     1261 atcacccggg aagacgacag agaccgaaga ttggctactg taaagcagtt gaaagaattt
     1321 attcagcaac cagaaaataa gctggtacta gttaaacaat tggataatat cttggctgct
     1381 gtacatgacg tgcttaatga aagtagcaaa ttgcttcagg agttgagaca ggagggagct
     1441 tgctgtcttg gccttctttg tgcttctctg agctatgagg ctgagaagat cttcaagtgg
     1501 atttttagca aatttagctc atctgcaaaa gatgaagtta aactcctcta cttatgtgcc
     1561 acctacaaag cactagagac tgtaggagaa aagaaagcct tttcatctgt aatgcagctt
     1621 gtaatgacca gcctgcagtc tattcttgaa aatgtggata caccagaatt gctttgtaaa
     1681 tgtgttaagt gcattctttt ggtggctcga tgttaccctc atattttcag cactaatttt
     1741 agggatacag ttgatatatt agttggatgg catatagatc atactcagaa accttcgctc
     1801 acgcagcagg tatctgggtg gttgcagagt ttggagccat tttgggtagc tgatcttgca
     1861 ttttctacta ctcttcttgg tcagtttctg gaagacatgg aagcatatgc tgaggacctc
     1921 agccatgtgg cctctgggga atcagtggat gaagatgtcc ctcctccatc agtgtcatta
     1981 ccaaagctgg ctgcacttct ccgggtattt agtactgtgg tgaggagcat tggggaacgc
     2041 ttcagcccaa ttcggggtcc tccaattact gaggcatatg taacagatgt tctgtacaga
     2101 gtaatgagat gtgtgacggc tgcaaaccag gtgttttttt ctgaggctgt gttgacagct
     2161 gctaatgagt gtgttggtgt tttgctcggc agcttggatc ctagcatgac tatacattgt
     2221 gacatggtca ttacatatgg attagaccaa ctggagaatt gccagacttg tggtaccgat
     2281 tatatcatct cagtcttgaa tttactcacg ctgattgttg aacagataaa tacgaaactg
     2341 ccatcatcat ttgtagaaaa actgtttata ccatcatcta aactactatt cttgcgttat
     2401 cataaagaaa aagaggttgt tgctgtagcc catgctgttt atcaagcagt gctcagcttg
     2461 aagaatattc ctgttttgga gactgcctat aagttaatat tgggagaaat gacttgtgcc
     2521 ctaaacaacc tcctacacag tctacaactt cctgaggcct gttctgaaat aaaacatgag
     2581 gcttttaaga atcatgtgtt caatgtagac aatgcaaaat ttgtagttat atttgacctc
     2641 agtgccctga ctacaattgg aaatgccaaa aactcactaa tagggatgtg ggcgctatct
     2701 ccaactgtct ttgcacttct gagtaagaat ctgatgattg tgcacagtga cctggctgtt
     2761 cacttccctg ccattcagta tgctgtgctc tacacattgt attctcattg taccaggcat
     2821 gatcacttta tctctagtag cctcagttct tcctctcctt ctttgtttga tggagctgtg
     2881 attagcactg taactacggc tacaaagaaa catttctcaa ttatattaaa tcttctggga
     2941 atattactta agaaagataa ccttaaccag gacacgagga aactgttaat gacttgggct
     3001 ttggaagcag ctgttttaat gaagaagtct gaaacatacg cacctttatt ctctcttccg
     3061 tctttccata aattttgcaa aggcctttta gccaacactc tcgttgaaga tgtgaatatc
     3121 tgtctgcagg catgcagcag tctacatgct ctgtcctctt ccttgccaga tgatctttta
     3181 cagagatgtg tcgatgtttg ccgtgttcaa ctagtgcaca gtggaactcg tattcgacaa
     3241 gcatttggaa aactgttgaa atcaattcct ttagatgttg tcctaagcaa taacaatcac
     3301 acagaaattc aagaaatttc tttagcatta agaagtcaca tgagtaaagc accaagtaat
     3361 acattccacc cccaagattt ctctgatgtt attagtttta ttttgtatgg gaactctcat
     3421 agaacaggga aggacaattg gttggaaaga ctgttctata gctgccagag actggataag
     3481 cgtgaccagt caacaattcc acgcaatctc ctgaagacag atgctgtcct ttggcagtgg
     3541 gccatatggg aagctgcaca attcactgtt ctttctaagc tgagaacccc actgggcaga
     3601 gctcaagaca ccttccagac aattgaaggt atcattcgaa gtctcgcagc tcacacatta
     3661 aaccctgatc aggatgttag tcagtggaca actgcagaca atgatgaagg ccatggtaac
     3721 aaccaactta gacttgttct tcttctgcag tatctggaaa atctggagaa attaatgtat
     3781 aatgcatacg agggatgtgc taatgcatta acttcacctc ccaaggtcat tagaactttt
     3841 ttctatacca atcgccaaac ttgtcaggac tggctaacgc ggattcgact ctccatcatg
     3901 agggtaggat tgttggcagg ccagcctgca gtgacagtga gacatggctt tgacttgctt
     3961 acagagatga aaacaaccag cctatctcag gggaatgaat tggaagtaac cattatgatg
     4021 gtggtagaag cattatgtga acttcattgt cctgaagcta tacagggaat tgctgtctgg
     4081 tcatcatcta ttgttggaaa aaatcttctg tggattaact cagtggctca acaggctgaa
     4141 gggaggtttg aaaaggcctc tgtggagtac caggaacacc tgtgtgccat gacaggtgtt
     4201 gattgctgca tctccagctt tgacaaatcg gtgctcacct tagccaatgc tgggcgtaac
     4261 agtgccagcc cgaaacattc tctgaatggt gaatccagaa aaactgtgct gtccaaaccg
     4321 actgactctt cccctgaggt tataaattat ttaggaaata aagcatgtga gtgctacatc
     4381 tcaattgccg attgggctgc tgtgcaggaa tggcagaacg ctatccatga cttgaaaaag
     4441 agtaccagta gcacttccct caacctgaaa gctgacttca actatataaa atcattaagc
     4501 agctttgagt ctggaaaatt tgttgaatgt accgagcagt tagaattgtt accaggagaa
     4561 aatatcaatc tacttgctgg aggatcaaaa gaaaaaatag acatgaaaaa actgcttcct
     4621 aacatgttaa gtccggatcc gagggaactt cagaaatcca ttgaagttca attgttaaga
     4681 agttctgttt gtttggcaac tgctttaaac ccgatagaac aagatcagaa gtggcagtct
     4741 ataactgaaa atgtggtaaa gtacttgaag caaacatccc gcatcgctat tggacctctg
     4801 agactttcta ctttaacagt ttcacagtct ttgccagttc taagtacctt gcagctgtat
     4861 tgctcatctg ctttggagaa cacagtttct aacagacttt caacagagga ctgtcttatt
     4921 ccactcttca gtgaagcttt acgttcatgt aaacagcatg acgtgaggcc atggatgcag
     4981 gcattaaggt atactatgta ccagaatcag ttgttggaga aaattaaaga acaaacagtc
     5041 ccaattagaa gccatctcat ggaattaggt ctaacagcag caaaatttgc tagaaaacga
     5101 gggaatgtgt cccttgcaac aagactgctg gcacagtgca gtgaagttca gctgggaaag
     5161 accaccactg cacaggattt agtccaacat tttaaaaaac tatcaaccca aggtcaagtg
     5221 gatgaaaaat gggggcccga acttgatatt gaaaaaacca aattgcttta tacagcaggc
     5281 cagtcaacac atgcaatgga aatgttgagt tcttgtgcca tatctttctg caagtctgtg
     5341 aaagctgaat atgcagttgc taaatcaatt ctgacactgg ctaaatggat ccaggcagaa
     5401 tggaaagaga tttcaggaca gctgaaacag gtttacagag ctcagcacca acagaacttc
     5461 acaggtcttt ctactttgtc taaaaacata ctcactctaa tagaactgcc atctgttaat
     5521 acgatggaag aagagtatcc tcggatcgag agtgaatcta cagtgcatat tggagttgga
     5581 gaacctgact tcattttggg acagttatat cacctgtctt cagtacaggc acctgaagta
     5641 gccaaatctt gggcagcgtt ggccagctgg gcttataggt ggggcagaaa ggtggttgac
     5701 aatgccagtc agggagaagg tgttcgtctg ctgcctagag aaaaatctga agttcagaat
     5761 ctacttccag acactataac tgaggaagag aaagagagaa tatatggtat tcttggacag
     5821 gctgtgtgtc ggccggcggg gattcaggat gaagatataa cacttcagat aactgagagt
     5881 gaagacaacg aagaagatga catggttgat gttatctggc gtcagttgat atcaagctgc
     5941 ccatggcttt cagaacttga tgaaagtgca actgaaggag ttattaaagt gtggaggaaa
     6001 gttgtagata gaatattcag cctgtacaaa ctctcttgca gtgcatactt tactttcctt
     6061 aaactcaacg ctggtcaaat tcctttagat gaggatgacc ctaggctgca tttaagtcac
     6121 agagtggaac agagcactga tgacatgatt gtgatggcca cattgcgcct gctgcggttg
     6181 ctcgtgaagc atgctggtga gcttcggcag tatctggagc acggcttgga gacaacaccc
     6241 actgcaccat ggaggggaat tattccgcaa cttttctcac gcttaaacca ccctgaagtg
     6301 tatgtgcgcc aaagtatttg taaccttctc tgccgtgtgg ctcaagattc cccacatctc
     6361 atattgtatc ctgcaatagt gggtaccata tcgcttagta gtgaatccca ggcttcagga
     6421 aataaatttt ccactgcaat tccaacttta cttggcaata ttcaaggaga agaattgctg
     6481 gtttctgaat gtgagggagg aagtcctcct gcatctcagg atagcaataa ggatgaacct
     6541 aaaagtggat taaatgaaga ccaagccatg atgcaggatt gttacagcaa aattgtagat
     6601 aagctgtcct ctgcaaaccc caccatggta ttacaggttc agatgctcgt ggctgaactg
     6661 cgcagggtca ctgtgctctg ggatgagctc tggctgggag ttttgctgca acaacacatg
     6721 tatgtcctga gacgaattca gcagcttgaa gatgaggtga agagagtcca gaacaacaac
     6781 accttacgca aagaagagaa aattgcaatc atgagggaga agcacacagc tttgatgaag
     6841 cccatcgtat ttgctttgga gcatgtgagg agtatcacag cggctcctgc agaaacacct
     6901 catgaaaaat ggtttcagga taactatggt gatgccattg aaaatgccct agaaaaactg
     6961 aagactccat tgaaccctgc aaagcctggg agcagctgga ttccatttaa agagataatg
     7021 ctaagtttgc aacagagagc acagaaacgt gcaagttaca tcttgcgtct tgaagaaatc
     7081 agtccatggt tggctgccat gactaacact gaaattgctc ttcctgggga agtctcagcc
     7141 agagacactg tcacaatcca tagtgtgggc ggaaccatca caatcttacc gactaaaacc
     7201 aagccaaaga aacttctctt tcttggatca gatgggaaga gctatcctta tcttttcaaa
     7261 ggactggagg atttacatct ggatgagaga ataatgcagt tcctatctat tgtgaatacc
     7321 atgtttgcta caattaatcg ccaagaaaca ccccggttcc atgctcgaca ctattctgta
     7381 acaccactag gaacaagatc aggactaatc cagtgggtag atggagccac acccttattt
     7441 ggtctttaca aacgatggca acaacgggaa gctgccttac aagcacaaaa ggcccaagat
     7501 tcctaccaaa ctcctcagaa tcctggaatt gtaccccgtc ctagtgaact ttattacagt
     7561 aaaattggcc ctgctttgaa aacagttggg cttagcctgg atgtgtcccg tcgggattgg
     7621 cctcttcatg taatgaaggc agtattggaa gagttaatgg aggccacacc cccgaatctc
     7681 cttgccaaag agctctggtc atcttgcaca acacctgatg aatggtggag agttacgcag
     7741 tcttatgcaa gatctactgc agtcatgtct atggttggat acataattgg ccttggagac
     7801 agacatctgg ataatgttct tatagatatg acgactggag aagttgttca catagattac
     7861 aatgtttgct ttgaaaaagg taaaagcctt agagttcctg agaaagtacc ttttcgaatg
     7921 acacaaaaca ttgaaacagc actgggtgta actggagtag aaggtgtatt taggctttca
     7981 tgtgagcagg ttttacacat tatgcggcgt ggcagagaga ccctgctgac gctgctggag
     8041 gcctttgtgt acgaccctct ggtggactgg acagcaggag gcgaggctgg gtttgctggt
     8101 gctgtctatg gtggaggtgg ccagcaggcc gagagcaagc agagcaagag agagatggag
     8161 cgagagatca cccgcagcct gttttcttct agagtagctg agattaaggt gaactggttt
     8221 aagaatagag atgagatgct ggttgtgctt cccaagttgg acggtagctt agatgaatac
     8281 ctaagcttgc aagagcaact gacagatgtg gaaaaactgc agggcaaact actggaggaa
     8341 atagagtttc tagaaggagc tgaaggggtg gatcatcctt ctcatactct gcaacacagg
     8401 tattctgagc acacccaact acagactcag caaagagctg ttcaggaagc aatccaggtg
     8461 aagctgaatg aatttgaaca atggataaca cattatcagg ctgcattcaa taatttagaa
     8521 gcaacacagc ttgcaagctt gcttcaagag ataagcacac aaatggacct tggtcctcca
     8581 agttacgtgc cagcaacagc ctttctgcag aatgctggtc aggcccactt gattagccag
     8641 tgcgagcagc tggaggggga ggttggtgct ctcctgcagc agaggcgctc cgtgctccgt
     8701 ggctgtctgg agcaactgca tcactatgca accgtggccc tgcagtatcc gaaggccata
     8761 tttcagaaac atcgaattga acagtggaag acctggatgg aagagctcat ctgtaacacc
     8821 acagtagagc gttgtcaaga gctctatagg aaatatgaaa tgcaatatgc tccccagcca
     8881 cccccaacag tgtgtcagtt catcactgcc actgaaatga ccctgcagcg atacgcagca
     8941 gacatcaaca gcagacttat tagacaagtg gaacgcttga aacaggaagc tgtcactgtg
     9001 ccagtttgtg aagatcagtt gaaagaaatt gaacgttgca ttaaagtttt ccttcatgag
     9061 aatggagaag aaggatcttt gagtctagca agtgttatta tttctgccct ttgtaccctt
     9121 acaaggcgta acctgatgat ggaaggtgca gcgtcaagtg ctggagaaca gctggttgat
     9181 ctgacttctc gggatggagc ctggttcttg gaggaactct gcagtatgag cggaaacgtc
     9241 acctgcttgg ttcagttact gaagcagtgc cacctggtgc cacaggactt agatatcccg
     9301 aaccccatgg aagcgtctga gacagttcac ttagccaatg gagtgtatac ctcacttcag
     9361 gaattgaatt cgaatttccg gcaaatcata tttccagaag cacttcgatg tttaatgaaa
     9421 ggggaataca cgttagaaag tatgctgcat gaactggacg gtcttattga gcagaccacc
     9481 gatggcgttc ccctgcagac tctagtggaa tctcttcagg cctacttaag aaacgcagct
     9541 atgggactgg aagaagaaac acatgctcat tacatcgatg ttgccagact actacatgct
     9601 cagtacggtg aattaatcca accgagaaat ggttcagttg atgaaacacc caaaatgtca
     9661 gctggccaga tgcttttggt agcattcgat ggcatgtttg ctcaagttga aactgctttc
     9721 agcttattag ttgaaaagtt gaacaagatg gaaattccca tagcttggcg aaagattgac
     9781 atcataaggg aagccaggag tactcaagtt aatttttttg atgatgataa tcaccggcag
     9841 gtgctagaag agattttctt tctaaaaaga ctacagacta ttaaggagtt cttcaggctc
     9901 tgtggtacct tttctaaaac attgtcagga tcaagttcac ttgaagatca gaatactgtg
     9961 aatgggcctg tacagattgt caatgtgaaa acccttttta gaaactcttg tttcagtgaa
    10021 gaccaaatgg ccaaacctat caaggcattc acagctgact ttgtgaggca gctcttgata
    10081 gggctaccca accaagccct cggactcaca ctgtgcagtt ttatcagtgc tctgggtgta
    10141 gacatcattg ctcaagtaga ggcaaaggac tttggtgccg aaagcaaagt ttctgttgat
    10201 gatctctgta agaaagcggt ggaacataac atccagatag ggaagttctc tcagctggtt
    10261 atgaacaggg caactgtgtt agcaagttct tacgacactg cctggaagaa gcatgacttg
    10321 gtgcgaaggc tagaaaccag tatttcttct tgtaagacaa gcctgcagcg ggttcagctg
    10381 catattgcca tgtttcagtg gcaacatgaa gatctactta tcaatagacc acaagccatg
    10441 tcagtcacac ctcccccacg gtctgctatc ctaaccagca tgaaaaagaa gctgcatacc
    10501 ctgagccaga ttgaaacttc tattgcgaca gttcaggaga agctagctgc acttgaatca
    10561 agtattgaac agcgactcaa gtgggcaggt ggtgccaacc ctgcattggc ccctgtacta
    10621 caagattttg aagcaacgat agctgaaaga agaaatcttg tccttaaaga gagccaaaga
    10681 gcaagtcagg tcacatttct ctgcagcaat atcattcatt ttgaaagttt acgaacaaga
    10741 actgcagaag ccttaaacct ggatgcggcg ttatttgaac taatcaagcg atgtcagcag
    10801 atgtgttcgt ttgcatcaca gtttaacagt tcagtgtctg agttagagct tcgtttatta
    10861 cagagagtgg acactggtct tgaacatcct attggcagct ctgaatggct tttgtcagca
    10921 cacaaacagt tgacccagga tatgtctact cagagggcaa ttcagacaga gaaagagcag
    10981 cagatagaaa cggtctgtga aacaattcag aatctggttg ataatataaa gactgtgctc
    11041 actggtcata accgacagct tggagatgtc aaacatctct tgaaagctat ggctaaggat
    11101 gaagaagctg ctctggcaga tggtgaagat gttccctatg agaacagtgt taggcagttt
    11161 ttgggtgaat ataaatcatg gcaagacaac attcaaacag ttctatttac attagtccag
    11221 gctatgggtc aggttcgaag tcaagaacac gttgaaatgc tccaggaaat cactcccacc
    11281 ttgaaagaac tgaaaacaca aagtcagagt atctataata atttagtgag ttttgcatca
    11341 cccttagtca ccgatgcaac aaatgaatgt tcgagtccaa cgtcatctgc tacttatcag
    11401 ccatccttcg ctgcagcagt ccggagtaac actggccaga aggctcagcc tgatgtcatg
    11461 gcacagaatg ctagaaagct gatccagaaa aatcttgcta catcagctga tactccacca
    11521 agcaccgttc caggaactgg caagagtgtt gcttgtagtc ctaaaaaggc agtcagagac
    11581 cctaaaactg ggaaagcggt gcaagagaga aactcctatg cagtgagtgt gtggaagaga
    11641 gtgaaagcca agttagaggg ccgagatgtt gatccgaata ggaggatgtc agttgctgaa
    11701 caggttgact atgtcattaa ggaagcaact aatctagata acttggctca gctgtatgaa
    11761 ggttggacag cctgggtgtg aatggcaaga cagtagatga gtctggttaa gcgaggtcag
    11821 acatccacca gaatcaactc gacctcgact cgactctaga ggatcccggg tggcatccct
    11881 gtgacccctc cccagtgcct ctcctggccc tggaagttgc cactccagtg cccaccagcc
    11941 ttgtcctaat aaaattaagt tgcatcattt tgtctgacta ggtgtccttc tataatatta
    12001 tggggtggag gggggtggta tggagcaagg ggcaagttgg gaagacaacc tgtagggcct
    12061 gcggggtcta ttgggaacca agctggagtg cagtggcaca atcttggctc actgcaatct
    12121 ccgcctcctg ggttcaagcg attctcctgc ctcagcctcc cgagttgttg ggattccagg
    12181 catgcatgac caggctcagc taatttttgt ttttttggta gagacggggt ttcaccatat
    12241 tggccaggct ggtctccaac tcctaatctc aggtgatcta cccaccttgg cctcccaaat
    12301 tgctgggatt acaggcgtga accactgctc ccttccctgt ccttctgatt ttaaaataac
    12361 tataccagca ggaggacgtc cagacacagc ataggctacc tggccatgcc caaccggtgg
    12421 gacatttgag ttgcttgctt ggcactgtcc tctcatgcgt tgggtccact cagtagatgc
    12481 ctgttgaatt gggtacgcgg ccagcttggc tgtggaatgt gtgtcagtta gggtgtggaa
    12541 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
    12601 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
    12661 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
    12721 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
    12781 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
    12841 tttgcaaaaa gctcctcgag gaactgaaaa accagaaagt taattcccta tagtgagtcg
    12901 tattaaattc gtaatcatgt catagctgtt tcctgtgtga aattgttatc cgctcacaat
    12961 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
    13021 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
    13081 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
    13141 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
    13201 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
    13261 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
    13321 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
    13381 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
    13441 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
    13501 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
    13561 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
    13621 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
    13681 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
    13741 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
    13801 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
    13861 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
    13921 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
    13981 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
    14041 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
    14101 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
    14161 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
    14221 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
    14281 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
    14341 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
    14401 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
    14461 aaggcgagtt acatgatccc ccatgttatg caaaaaagcg gttagctcct tcggtcctcc
    14521 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
    14581 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
    14641 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
    14701 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
    14761 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
    14821 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
    14881 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
    14941 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
    15001 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
    15061 agtgccacct gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
    15121 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
    15181 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
    15241 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
    15301 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
    15361 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
    15421 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
    15481 acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttcca ttcgccattc
    15541 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
    15601 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
    15661 cgacgttgta aaacgacggc cagtgccaag ctgatctata cattgaatca atattggcaa
    15721 ttagccatat tagtcattgg ttatatagca taaatcaata tt
//
