LOCUS       sequence-391051-       12249 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  dual fluorescent reporter for MYH6 (short) and TNNT2 promoters.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12249)
  AUTHORS   Romero-Tejeda M, Fonoudi H, Weddle CJ, DeKeyser JM, Lenny B, 
            Fetterman KA, Magdy T, Sapkota Y, Epting C, Burridge PW
  TITLE     A novel transcription factor combination for direct reprogramming to
            a spontaneously contracting human cardiomyocyte-like state.
  JOURNAL   J Mol Cell Cardiol. 2023 Jul 6:S0022-2828(23)00106-2. doi: 
            10.1016/j.yjmcc.2023.06.005.
  PUBMED    37421991
REFERENCE   2  (bases 1 to 12249)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol Cell 
            Cardiol. 2023 Jul 6:S0022-2828(23)00106-2. doi: 
            10.1016/j.yjmcc.2023.06.005."
COMMENT     Sequence Label: pFU-GW-sMYH6-mNeon-TNNT2-mScarlet
FEATURES             Location/Qualifiers
     source          1..12249
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     regulatory      4350..4359
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4356..5066
                     /codon_start=1
                     /product="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
                     /label=mNeonGreen
                     /translation="MVSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYE
                     ELNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFED
                     GASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIS
                     TFKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQ
                     KAFTDVMGMDELYK"
     regulatory      4611..4620
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(5157..5176)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5222..5277
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5276..5295)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     regulatory      6212..6221
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6218..6913
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     complement(6351..6369)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6619..6638
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    6941..7529
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6994..7014)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7412..7423)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7532..7548)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7533..7549)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8054..8234
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8260..8277)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8266..8490
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8536..8964
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8623..8642)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8833..8854
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8973..8993)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8978..9307
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9158..9293
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9220..9239
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9355..9402
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9421..9795
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9925..10046
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9962..9981)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10016..10035
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10095..10111)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10095..10111)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10108..10130)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10119..10135
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10143..10149,10150..10167,10168..10173))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10188..10209
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10326..10343)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10497..11085)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10577..10596)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11256..12047,12048..12116))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11879..11898
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12117..12221)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta aagagactac agacagtatc tgtacagagt aaggagagag
     2641 aggagggggt gtagaattct cttactatca aagggaaact gagtcgtgca cctgcaaagt
     2701 ggatgctctc cctagacatc atgactttgt ctctggggag ccagcactgt ggaacttcag
     2761 gtctgagaga gtaggaggct cccctcagcc tgaagctatg cagatagcca gggttgaaag
     2821 ggggaaggga gagcctggga tgggagcttg tgtgttggag gcaggggaca gatattaagc
     2881 ctggaagaga aggtgaccct tacccagttg ttcaactcac ccttcagatt aaaaataact
     2941 gaggtaaggg cctgggtagg ggaggtggtg tgagacgctc ctgtctctcc tctatctgcc
     3001 catcggccct ttggggagga ggaatgtgcc caaggactaa aaaaaggcca tggagccaga
     3061 ggggcgaggg caacagacct ttcatgggca aaccttgggg ccctgctgtc ctcctgtcac
     3121 ctccagagcc aagggatcaa aggaggagga gccaggacag gagggaagtg ggagggaggg
     3181 tcccagcaga ggactccaaa tttaggcagc aggcatatgg gatgggatat aaaggggctg
     3241 gagcactgag agctgtcaga gatttctcca acccaggtaa gagggagttt cgggtggggg
     3301 ctcttcaccc acaccagacc tctccccacc tagaaggaaa ctgcctttcc tggaagtggg
     3361 gttcaggccg gtcagagatc tgacagggtg gccttccacc agcctgggaa gttctcagtg
     3421 gcaggaggtt tccacaagaa acactggatg ccccttccct tacgctgtct tctccatctt
     3481 cctcctgggg atgctcctcc ccgtcttggt ttatcttggc tcttcgtctt cagcaagatt
     3541 tgccctgtgc tgtccactcc atctttctct actgtctccg tgccttgcct tgccttcttg
     3601 cgtgtccttc ctttccaccc atttctcact tcaccttttc tccccttctc atttgtattc
     3661 atccttcctt ccttccttcc ttccttcctt ccttccttcc ttccttcctt tctcccttcc
     3721 ttccttcctt ccttccttcc ttccttcctt ccttcctgtg tcagagtgct gagaatcaca
     3781 cctggggttc ccacccttat gtaaacaatc ttccagtgag ccacagcttc agtgctgctg
     3841 ggtgctctct taccttcctc accccctggc ttgtcctgtt ccatcctggt caggatctct
     3901 agattggtct cccagcctct gctactcctc ttcctgcctg ttcctctctc tgtccagctg
     3961 cgccactgtg gtgcctcgtt ccagctgtgg tccacattct tcaggattct ctgaaaagtt
     4021 aaccaggtga gaatgtttcc cctgtagaca gcagatcacg attctcccgg aagtcaggct
     4081 tccagccctc tctttctctg cccagctgcc cggcactctt agcaaacctc aggcaccctt
     4141 accccacata gacctctgac agagaagcag gcactttaca tggagtcctg gtgggagagc
     4201 cataggctac ggtgtaaaag aggcagggaa gtggtggtgt aggaaagtca ggacttcaca
     4261 tagaagccta gcccacacca gaaatgacag acagatccct cctatctccc ccataagagt
     4321 ttgagtcgac tgatcaaaaa ccggtcgcgg ccgccatggt gagcaagggc gaggaggaca
     4381 acatggccag cctgccagcg acacatgagt tacacatctt tggctccatc aacggtgtgg
     4441 actttgacat ggtgggtcag ggcaccggca atccaaatga tggttatgag gagttaaacc
     4501 tgaagtccac caagggtgac ctccagttct ccccctggat tctggtccct catatcgggt
     4561 atggcttcca tcagtacctg ccctaccctg acgggatgtc gcctttccag gccgccatgg
     4621 tagatggctc cggataccaa gtccatcgca caatgcagtt tgaagatggt gcctccctta
     4681 ctgttaacta ccgctacacc tacgagggaa gccacatcaa aggagaggcc caggtgaagg
     4741 ggactggttt ccctgctgac ggtcctgtga tgaccaactc gctgaccgct gcggactggt
     4801 gcaggtcgaa gaagacttac cccaacgaca aaaccatcat cagtaccttt aagtggagtt
     4861 acaccactgg aaatggcaag cgctacagga gcactgcgcg gaccacctac acctttgcca
     4921 agccaatggc ggctaactat ctgaagaacc agccgatgta cgtgttccgt aagacggagc
     4981 tcaagcactc caagaccgag ctcaacttca aggagtggca aaaggccttt accgatgtga
     5041 tgggcatgga cgagctgtac aagtgacctg caggaattca ctcctcaggt gcaggctgcc
     5101 tatcagaagg tggtggctgg tgtggccaat gccctggctc acaaatacca ctgagatctt
     5161 tttccctctg ccaaaaatta tggggacatc atgaagcccc ttgagcatct gacttctggc
     5221 taataaagga aatttatttt cattgcaata gtgtgttgga attttttgtg tctctcactc
     5281 ggaaggacat atgggagggc aaatcattta aaacatcaga atgagtattt ggtttagagt
     5341 ttggcaacat atgcccatat gctggctgcc atgaacaaag gttggctata aagaggtcat
     5401 cagtatatga aacagccccc tgctgtccat tccttattcc atagaaaagc cttgacttga
     5461 ggttagattt tttttatatt ttgttttgtg ttattttttt ctttaacatc cctaaaattt
     5521 tccttacatg ttttactagc cagatttttc ctcctctcct gactactccc agtcatagct
     5581 gtccctcttc tcttatgaag atcgtcatgg agaagaccca ccttgcagat gtcctcactg
     5641 gggctggcag agccggcaac ctgcccaagg ctgctcagtc cattaggagc cagtagcctg
     5701 gaagatgtct ttacccccag catcagttca agtggagcag cacataactc ttgccctctg
     5761 ccttccaaga ttctggtgct gagacttatg gagtgtcttg gaggttgcct tctgcccccc
     5821 aaccctgctc ccagctggcc ctcccaggcc tgggttgctg gcctctgctt tatcaggatt
     5881 ctcaagaggg acagctggtt tatgttgcat gactgttccc tgcatatctg ctctggtttt
     5941 aaatagctta tctgagcagc tggaggacca catgggctta tatggcgtgg ggtacatgtt
     6001 cctgtagcct tgtccctggc acctgccaaa atagcagcca acacccccca cccccaccgc
     6061 catccccctg ccccacccgt cccctgtcgc acattcctcc ctccgcaggg ctggctcacc
     6121 aggccccagc ccacatgcct gcttaaagcc ctctccatcc tctgcctcac ccagtccccg
     6181 ctgagactga gcagacgcct ccaggatcgc ggccgccatg gtgagcaagg gcgaggcagt
     6241 gatcaaggag ttcatgcggt tcaaggtgca catggagggc tccatgaacg gccacgagtt
     6301 cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa
     6361 ggtgaccaag ggtggccccc tgcccttctc ctgggacatc ctgtcccctc agttcatgta
     6421 cggctccagg gccttcacca agcaccccgc cgacatcccc gactactata agcagtcctt
     6481 ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg ccgtgaccgt
     6541 gacccaggac acctccctgg aggacggcac cctgatctac aaggtgaagc tccgcggcac
     6601 caacttccct cctgacggcc ccgtaatgca gaagaagaca atgggctggg aagcgtccac
     6661 cgagcggttg taccccgagg acggcgtgct gaagggcgac attaagatgg ccctgcgcct
     6721 gaaggacgga ggccgctacc tggcggactt caagaccacc tacaaggcca agaagcccgt
     6781 gcagatgccc ggcgcctaca acgtcgaccg caagttggac atcacctccc acaacgagga
     6841 ctacaccgtg gtggaacagt acgaacgctc cgagggccgc cactccaccg gcggcatgga
     6901 cgagctgtac aagtaagaat tcgatatcaa gcttatcgat aatcaacctc tggattacaa
     6961 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     7021 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     7081 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     7141 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     7201 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
     7261 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     7321 ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat
     7381 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     7441 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
     7501 tcggatctcc ctttgggccg cctccccgca tcgataccgt cgacctcgag acctagaaaa
     7561 acatggagca atcacaagta gcaatacagc agctaccaat gctgattgtg cctggctaga
     7621 agcacaagag gaggaggagg tgggttttcc agtcacacct caggtacctt taagaccaat
     7681 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg
     7741 gctaattcac tcccaacgaa gacaagatat ccttgatctg tggatctacc acacacaagg
     7801 ctacttccct gattggcaga actacacacc agggccaggg atcagatatc cactgacctt
     7861 tggatggtgc tacaagctag taccagttga gcaagagaag gtagaagaag ccaatgaagg
     7921 agagaacacc cgcttgttac accctgtgag cctgcatggg atggatgacc cggagagaga
     7981 agtattagag tggaggtttg acagccgcct agcatttcat cacatggccc gagagctgca
     8041 tccggactgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     8101 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     8161 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     8221 gaaaatctct agcagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt
     8281 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
     8341 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
     8401 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca
     8461 ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct
     8521 ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     8581 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     8641 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     8701 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     8761 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     8821 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     8881 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     8941 tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa
     9001 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     9061 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     9121 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     9181 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     9241 cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     9301 ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcagc acgtgttgac
     9361 aattaatcat cggcatagta tatcggcata gtataatacg acaaggtgag gaactaaacc
     9421 atggccaagt tgaccagtgc cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc
     9481 gagttctgga ccgaccggct cgggttctcc cgggacttcg tggaggacga cttcgccggt
     9541 gtggtccggg acgacgtgac cctgttcatc agcgcggtcc aggaccaggt ggtgccggac
     9601 aacaccctgg cctgggtgtg ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag
     9661 gtcgtgtcca cgaacttccg ggacgcctcc gggccggcca tgaccgagat cggcgagcag
     9721 ccgtgggggc gggagttcgc cctgcgcgac ccggccggca actgcgtgca cttcgtggcc
     9781 gaggagcagg actgacacgt gctacgagat ttcgattcca ccgccgcctt ctatgaaagg
     9841 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc
     9901 atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa
     9961 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
    10021 ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc
    10081 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
    10141 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
    10201 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
    10261 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
    10321 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
    10381 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
    10441 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
    10501 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
    10561 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
    10621 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
    10681 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
    10741 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
    10801 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
    10861 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
    10921 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
    10981 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
    11041 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
    11101 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
    11161 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
    11221 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
    11281 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
    11341 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
    11401 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
    11461 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
    11521 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
    11581 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
    11641 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
    11701 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
    11761 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
    11821 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
    11881 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
    11941 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
    12001 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
    12061 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
    12121 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
    12181 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
    12241 ccacctgac
//
