LOCUS       sequence-391052-       15762 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses FLAG-tagged kinase dead SMG1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15762)
  AUTHORS   McCann JJ, Fleenor DE, Chen J, Lai CH, Bass TE, Kastan MB
  TITLE     Participation of ATM, SMG1, and DDX5 in a DNA Damage-Induced 
            Alternative Splicing Pathway.
  JOURNAL   Radiat Res. 2023 Apr 1;199(4):406-421. doi: 10.1667/RADE-22-00219.1.
  PUBMED    36921295
REFERENCE   2  (bases 1 to 15762)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1667/RADE-22-00219"; journalName: "Radiat Res"; date: 
            "2023-04-1- 1"; volume: "199"; issue: "4"; pages: "406-421"
COMMENT     Sequence Label: FLAG SMG1 D2335A/N2340K
FEATURES             Location/Qualifiers
     source          1..15762
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        81..460
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        461..664
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     615..635
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             698..721
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    6086..6097
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     polyA_signal    11866..12488
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(12065..12084)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(12512..12532)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        12517..12846
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      12697..12832
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(12884..12903)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(12885..12903)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(12917..12933)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12917..12933)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12930..12952)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12941..12957
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12965..12971,12972..12989,12990..12995))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13010..13031
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(13148..13165)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13319..13907)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13399..13418)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(14078..14869,14870..14938))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14701..14720
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14939..15043)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      15070..15525
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(15157..15176)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     15367..15388
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     15651..15673
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     15665..15682
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     15666..15682
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ctattggcca ttgcatacgt tgtatctata tcataatatg tacatttata ttggctcatg
       61 tccaatatga ccgccatgtt gacattgatt attgactagt tattaatagt aatcaattac
      121 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
      181 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
      241 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
      301 tgcccacttg gcagtacatc aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa
      361 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttacggg actttcctac
      421 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta
      481 caccaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga
      541 cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa
      601 ccccgccccg ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag
      661 agctcgttta gtgaaccgtc agaattgatc taccatggac tacaaagacg atgacgacaa
      721 gcttgcggcc gcgaattcat cgatagatct gatatcggta ccagtcgaga tccatgtcgt
      781 gtcggggaag gtaatgagcc gcagagcccc ggggtctcgg ctgagcagcg gcggcggcgg
      841 cggcggcacc aagtatccgc ggagctggaa tgactggcaa cccagaactg atagtgcatc
      901 agccgaccca gataatttaa aatattcttc atccagagat agaggtggtt cttcctctta
      961 cggactgcaa ccttcaaatt cagctgtggt gtctcggcaa aggcacgatg ataccagagt
     1021 ccacgctgac atacagaatg acgaaaaggg tggctacagt gtcaatggag gatctgggga
     1081 aaatacttat ggtcggaagt cgttggggca agagctgagg gttaacaatg tgaccagccc
     1141 tgagttcacc agtgttcagc atggcagtcg tgctttagcc accaaagaca tgaggaaatc
     1201 acaggagaga tcgatgtctt attctgatga gtctcgactg tcgaatcttc ttcggaggat
     1261 cacccgggaa gacgacagag accgaagatt ggctactgta aagcagttga aagaatttat
     1321 tcagcaacca gaaaataagc tggtactagt taaacaattg gataatatct tggctgctgt
     1381 acatgacgtg cttaatgaaa gtagcaaatt gcttcaggag ttgagacagg agggagcttg
     1441 ctgtcttggc cttctttgtg cttctctgag ctatgaggct gagaagatct tcaagtggat
     1501 ttttagcaaa tttagctcat ctgcaaaaga tgaagttaaa ctcctctact tatgtgccac
     1561 ctacaaagca ctagagactg taggagaaaa gaaagccttt tcatctgtaa tgcagcttgt
     1621 aatgaccagc ctgcagtcta ttcttgaaaa tgtggataca ccagaattgc tttgtaaatg
     1681 tgttaagtgc attcttttgg tggctcgatg ttaccctcat attttcagca ctaattttag
     1741 ggatacagtt gatatattag ttggatggca tatagatcat actcagaaac cttcgctcac
     1801 gcagcaggta tctgggtggt tgcagagttt ggagccattt tgggtagctg atcttgcatt
     1861 ttctactact cttcttggtc agtttctgga agacatggaa gcatatgctg aggacctcag
     1921 ccatgtggcc tctggggaat cagtggatga agatgtccct cctccatcag tgtcattacc
     1981 aaagctggct gcacttctcc gggtatttag tactgtggtg aggagcattg gggaacgctt
     2041 cagcccaatt cggggtcctc caattactga ggcatatgta acagatgttc tgtacagagt
     2101 aatgagatgt gtgacggctg caaaccaggt gtttttttct gaggctgtgt tgacagctgc
     2161 taatgagtgt gttggtgttt tgctcggcag cttggatcct agcatgacta tacattgtga
     2221 catggtcatt acatatggat tagaccaact ggagaattgc cagacttgtg gtaccgatta
     2281 tatcatctca gtcttgaatt tactcacgct gattgttgaa cagataaata cgaaactgcc
     2341 atcatcattt gtagaaaaac tgtttatacc atcatctaaa ctactattct tgcgttatca
     2401 taaagaaaaa gaggttgttg ctgtagccca tgctgtttat caagcagtgc tcagcttgaa
     2461 gaatattcct gttttggaga ctgcctataa gttaatattg ggagaaatga cttgtgccct
     2521 aaacaacctc ctacacagtc tacaacttcc tgaggcctgt tctgaaataa aacatgaggc
     2581 ttttaagaat catgtgttca atgtagacaa tgcaaaattt gtagttatat ttgacctcag
     2641 tgccctgact acaattggaa atgccaaaaa ctcactaata gggatgtggg cgctatctcc
     2701 aactgtcttt gcacttctga gtaagaatct gatgattgtg cacagtgacc tggctgttca
     2761 cttccctgcc attcagtatg ctgtgctcta cacattgtat tctcattgta ccaggcatga
     2821 tcactttatc tctagtagcc tcagttcttc ctctccttct ttgtttgatg gagctgtgat
     2881 tagcactgta actacggcta caaagaaaca tttctcaatt atattaaatc ttctgggaat
     2941 attacttaag aaagataacc ttaaccagga cacgaggaaa ctgttaatga cttgggcttt
     3001 ggaagcagct gttttaatga agaagtctga aacatacgca cctttattct ctcttccgtc
     3061 tttccataaa ttttgcaaag gccttttagc caacactctc gttgaagatg tgaatatctg
     3121 tctgcaggca tgcagcagtc tacatgctct gtcctcttcc ttgccagatg atcttttaca
     3181 gagatgtgtc gatgtttgcc gtgttcaact agtgcacagt ggaactcgta ttcgacaagc
     3241 atttggaaaa ctgttgaaat caattccttt agatgttgtc ctaagcaata acaatcacac
     3301 agaaattcaa gaaatttctt tagcattaag aagtcacatg agtaaagcac caagtaatac
     3361 attccacccc caagatttct ctgatgttat tagttttatt ttgtatggga actctcatag
     3421 aacagggaag gacaattggt tggaaagact gttctatagc tgccagagac tggataagcg
     3481 tgaccagtca acaattccac gcaatctcct gaagacagat gctgtccttt ggcagtgggc
     3541 catatgggaa gctgcacaat tcactgttct ttctaagctg agaaccccac tgggcagagc
     3601 tcaagacacc ttccagacaa ttgaaggtat cattcgaagt ctcgcagctc acacattaaa
     3661 ccctgatcag gatgttagtc agtggacaac tgcagacaat gatgaaggcc atggtaacaa
     3721 ccaacttaga cttgttcttc ttctgcagta tctggaaaat ctggagaaat taatgtataa
     3781 tgcatacgag ggatgtgcta atgcattaac ttcacctccc aaggtcatta gaactttttt
     3841 ctataccaat cgccaaactt gtcaggactg gctaacgcgg attcgactct ccatcatgag
     3901 ggtaggattg ttggcaggcc agcctgcagt gacagtgaga catggctttg acttgcttac
     3961 agagatgaaa acaaccagcc tatctcaggg gaatgaattg gaagtaacca ttatgatggt
     4021 ggtagaagca ttatgtgaac ttcattgtcc tgaagctata cagggaattg ctgtctggtc
     4081 atcatctatt gttggaaaaa atcttctgtg gattaactca gtggctcaac aggctgaagg
     4141 gaggtttgaa aaggcctctg tggagtacca ggaacacctg tgtgccatga caggtgttga
     4201 ttgctgcatc tccagctttg acaaatcggt gctcacctta gccaatgctg ggcgtaacag
     4261 tgccagcccg aaacattctc tgaatggtga atccagaaaa actgtgctgt ccaaaccgac
     4321 tgactcttcc cctgaggtta taaattattt aggaaataaa gcatgtgagt gctacatctc
     4381 aattgccgat tgggctgctg tgcaggaatg gcagaacgct atccatgact tgaaaaagag
     4441 taccagtagc acttccctca acctgaaagc tgacttcaac tatataaaat cattaagcag
     4501 ctttgagtct ggaaaatttg ttgaatgtac cgagcagtta gaattgttac caggagaaaa
     4561 tatcaatcta cttgctggag gatcaaaaga aaaaatagac atgaaaaaac tgcttcctaa
     4621 catgttaagt ccggatccga gggaacttca gaaatccatt gaagttcaat tgttaagaag
     4681 ttctgtttgt ttggcaactg ctttaaaccc gatagaacaa gatcagaagt ggcagtctat
     4741 aactgaaaat gtggtaaagt acttgaagca aacatcccgc atcgctattg gacctctgag
     4801 actttctact ttaacagttt cacagtcttt gccagttcta agtaccttgc agctgtattg
     4861 ctcatctgct ttggagaaca cagtttctaa cagactttca acagaggact gtcttattcc
     4921 actcttcagt gaagctttac gttcatgtaa acagcatgac gtgaggccat ggatgcaggc
     4981 attaaggtat actatgtacc agaatcagtt gttggagaaa attaaagaac aaacagtccc
     5041 aattagaagc catctcatgg aattaggtct aacagcagca aaatttgcta gaaaacgagg
     5101 gaatgtgtcc cttgcaacaa gactgctggc acagtgcagt gaagttcagc tgggaaagac
     5161 caccactgca caggatttag tccaacattt taaaaaacta tcaacccaag gtcaagtgga
     5221 tgaaaaatgg gggcccgaac ttgatattga aaaaaccaaa ttgctttata cagcaggcca
     5281 gtcaacacat gcaatggaaa tgttgagttc ttgtgccata tctttctgca agtctgtgaa
     5341 agctgaatat gcagttgcta aatcaattct gacactggct aaatggatcc aggcagaatg
     5401 gaaagagatt tcaggacagc tgaaacaggt ttacagagct cagcaccaac agaacttcac
     5461 aggtctttct actttgtcta aaaacatact cactctaata gaactgccat ctgttaatac
     5521 gatggaagaa gagtatcctc ggatcgagag tgaatctaca gtgcatattg gagttggaga
     5581 acctgacttc attttgggac agttatatca cctgtcttca gtacaggcac ctgaagtagc
     5641 caaatcttgg gcagcgttgg ccagctgggc ttataggtgg ggcagaaagg tggttgacaa
     5701 tgccagtcag ggagaaggtg ttcgtctgct gcctagagaa aaatctgaag ttcagaatct
     5761 acttccagac actataactg aggaagagaa agagagaata tatggtattc ttggacaggc
     5821 tgtgtgtcgg ccggcgggga ttcaggatga agatataaca cttcagataa ctgagagtga
     5881 agacaacgaa gaagatgaca tggttgatgt tatctggcgt cagttgatat caagctgccc
     5941 atggctttca gaacttgatg aaagtgcaac tgaaggagtt attaaagtgt ggaggaaagt
     6001 tgtagataga atattcagcc tgtacaaact ctcttgcagt gcatacttta ctttccttaa
     6061 actcaacgct ggtcaaattc ctttagatga ggatgaccct aggctgcatt taagtcacag
     6121 agtggaacag agcactgatg acatgattgt gatggccaca ttgcgcctgc tgcggttgct
     6181 cgtgaagcat gctggtgagc ttcggcagta tctggagcac ggcttggaga caacacccac
     6241 tgcaccatgg aggggaatta ttccgcaact tttctcacgc ttaaaccacc ctgaagtgta
     6301 tgtgcgccaa agtatttgta accttctctg ccgtgtggct caagattccc cacatctcat
     6361 attgtatcct gcaatagtgg gtaccatatc gcttagtagt gaatcccagg cttcaggaaa
     6421 taaattttcc actgcaattc caactttact tggcaatatt caaggagaag aattgctggt
     6481 ttctgaatgt gagggaggaa gtcctcctgc atctcaggat agcaataagg atgaacctaa
     6541 aagtggatta aatgaagacc aagccatgat gcaggattgt tacagcaaaa ttgtagataa
     6601 gctgtcctct gcaaacccca ccatggtatt acaggttcag atgctcgtgg ctgaactgcg
     6661 cagggtcact gtgctctggg atgagctctg gctgggagtt ttgctgcaac aacacatgta
     6721 tgtcctgaga cgaattcagc agcttgaaga tgaggtgaag agagtccaga acaacaacac
     6781 cttacgcaaa gaagagaaaa ttgcaatcat gagggagaag cacacagctt tgatgaagcc
     6841 catcgtattt gctttggagc atgtgaggag tatcacagcg gctcctgcag aaacacctca
     6901 tgaaaaatgg tttcaggata actatggtga tgccattgaa aatgccctag aaaaactgaa
     6961 gactccattg aaccctgcaa agcctgggag cagctggatt ccatttaaag agataatgct
     7021 aagtttgcaa cagagagcac agaaacgtgc aagttacatc ttgcgtcttg aagaaatcag
     7081 tccatggttg gctgccatga ctaacactga aattgctctt cctggggaag tctcagccag
     7141 agacactgtc acaatccata gtgtgggcgg aaccatcaca atcttaccga ctaaaaccaa
     7201 gccaaagaaa cttctctttc ttggatcaga tgggaagagc tatccttatc ttttcaaagg
     7261 actggaggat ttacatctgg atgagagaat aatgcagttc ctatctattg tgaataccat
     7321 gtttgctaca attaatcgcc aagaaacacc ccggttccat gctcgacact attctgtaac
     7381 accactagga acaagatcag gactaatcca gtgggtagat ggagccacac ccttatttgg
     7441 tctttacaaa cgatggcaac aacgggaagc tgccttacaa gcacaaaagg cccaagattc
     7501 ctaccaaact cctcagaatc ctggaattgt accccgtcct agtgaacttt attacagtaa
     7561 aattggccct gctttgaaaa cagttgggct tagcctggat gtgtcccgtc gggattggcc
     7621 tcttcatgta atgaaggcag tattggaaga gttaatggag gccacacccc cgaatctcct
     7681 tgccaaagag ctctggtcat cttgcacaac acctgatgaa tggtggagag ttacgcagtc
     7741 ttatgcaaga tctactgcag tcatgtctat ggttggatac ataattggcc ttggagccag
     7801 acatctggat aaagttctta tagatatgac gactggagaa gttgttcaca tagattacaa
     7861 tgtttgcttt gaaaaaggta aaagccttag agttcctgag aaagtacctt ttcgaatgac
     7921 acaaaacatt gaaacagcac tgggtgtaac tggagtagaa ggtgtattta ggctttcatg
     7981 tgagcaggtt ttacacatta tgcggcgtgg cagagagacc ctgctgacgc tgctggaggc
     8041 ctttgtgtac gaccctctgg tggactggac agcaggaggc gaggctgggt ttgctggtgc
     8101 tgtctatggt ggaggtggcc agcaggccga gagcaagcag agcaagagag agatggagcg
     8161 agagatcacc cgcagcctgt tttcttctag agtagctgag attaaggtga actggtttaa
     8221 gaatagagat gagatgctgg ttgtgcttcc caagttggac ggtagcttag atgaatacct
     8281 aagcttgcaa gagcaactga cagatgtgga aaaactgcag ggcaaactac tggaggaaat
     8341 agagtttcta gaaggagctg aaggggtgga tcatccttct catactctgc aacacaggta
     8401 ttctgagcac acccaactac agactcagca aagagctgtt caggaagcaa tccaggtgaa
     8461 gctgaatgaa tttgaacaat ggataacaca ttatcaggct gcattcaata atttagaagc
     8521 aacacagctt gcaagcttgc ttcaagagat aagcacacaa atggaccttg gtcctccaag
     8581 ttacgtgcca gcaacagcct ttctgcagaa tgctggtcag gcccacttga ttagccagtg
     8641 cgagcagctg gagggggagg ttggtgctct cctgcagcag aggcgctccg tgctccgtgg
     8701 ctgtctggag caactgcatc actatgcaac cgtggccctg cagtatccga aggccatatt
     8761 tcagaaacat cgaattgaac agtggaagac ctggatggaa gagctcatct gtaacaccac
     8821 agtagagcgt tgtcaagagc tctataggaa atatgaaatg caatatgctc cccagccacc
     8881 cccaacagtg tgtcagttca tcactgccac tgaaatgacc ctgcagcgat acgcagcaga
     8941 catcaacagc agacttatta gacaagtgga acgcttgaaa caggaagctg tcactgtgcc
     9001 agtttgtgaa gatcagttga aagaaattga acgttgcatt aaagttttcc ttcatgagaa
     9061 tggagaagaa ggatctttga gtctagcaag tgttattatt tctgcccttt gtacccttac
     9121 aaggcgtaac ctgatgatgg aaggtgcagc gtcaagtgct ggagaacagc tggttgatct
     9181 gacttctcgg gatggagcct ggttcttgga ggaactctgc agtatgagcg gaaacgtcac
     9241 ctgcttggtt cagttactga agcagtgcca cctggtgcca caggacttag atatcccgaa
     9301 ccccatggaa gcgtctgaga cagttcactt agccaatgga gtgtatacct cacttcagga
     9361 attgaattcg aatttccggc aaatcatatt tccagaagca cttcgatgtt taatgaaagg
     9421 ggaatacacg ttagaaagta tgctgcatga actggacggt cttattgagc agaccaccga
     9481 tggcgttccc ctgcagactc tagtggaatc tcttcaggcc tacttaagaa acgcagctat
     9541 gggactggaa gaagaaacac atgctcatta catcgatgtt gccagactac tacatgctca
     9601 gtacggtgaa ttaatccaac cgagaaatgg ttcagttgat gaaacaccca aaatgtcagc
     9661 tggccagatg cttttggtag cattcgatgg catgtttgct caagttgaaa ctgctttcag
     9721 cttattagtt gaaaagttga acaagatgga aattcccata gcttggcgaa agattgacat
     9781 cataagggaa gccaggagta ctcaagttaa tttttttgat gatgataatc accggcaggt
     9841 gctagaagag attttctttc taaaaagact acagactatt aaggagttct tcaggctctg
     9901 tggtaccttt tctaaaacat tgtcaggatc aagttcactt gaagatcaga atactgtgaa
     9961 tgggcctgta cagattgtca atgtgaaaac cctttttaga aactcttgtt tcagtgaaga
    10021 ccaaatggcc aaacctatca aggcattcac agctgacttt gtgaggcagc tcttgatagg
    10081 gctacccaac caagccctcg gactcacact gtgcagtttt atcagtgctc tgggtgtaga
    10141 catcattgct caagtagagg caaaggactt tggtgccgaa agcaaagttt ctgttgatga
    10201 tctctgtaag aaagcggtgg aacataacat ccagataggg aagttctctc agctggttat
    10261 gaacagggca actgtgttag caagttctta cgacactgcc tggaagaagc atgacttggt
    10321 gcgaaggcta gaaaccagta tttcttcttg taagacaagc ctgcagcggg ttcagctgca
    10381 tattgccatg tttcagtggc aacatgaaga tctacttatc aatagaccac aagccatgtc
    10441 agtcacacct cccccacggt ctgctatcct aaccagcatg aaaaagaagc tgcataccct
    10501 gagccagatt gaaacttcta ttgcgacagt tcaggagaag ctagctgcac ttgaatcaag
    10561 tattgaacag cgactcaagt gggcaggtgg tgccaaccct gcattggccc ctgtactaca
    10621 agattttgaa gcaacgatag ctgaaagaag aaatcttgtc cttaaagaga gccaaagagc
    10681 aagtcaggtc acatttctct gcagcaatat cattcatttt gaaagtttac gaacaagaac
    10741 tgcagaagcc ttaaacctgg atgcggcgtt atttgaacta atcaagcgat gtcagcagat
    10801 gtgttcgttt gcatcacagt ttaacagttc agtgtctgag ttagagcttc gtttattaca
    10861 gagagtggac actggtcttg aacatcctat tggcagctct gaatggcttt tgtcagcaca
    10921 caaacagttg acccaggata tgtctactca gagggcaatt cagacagaga aagagcagca
    10981 gatagaaacg gtctgtgaaa caattcagaa tctggttgat aatataaaga ctgtgctcac
    11041 tggtcataac cgacagcttg gagatgtcaa acatctcttg aaagctatgg ctaaggatga
    11101 agaagctgct ctggcagatg gtgaagatgt tccctatgag aacagtgtta ggcagttttt
    11161 gggtgaatat aaatcatggc aagacaacat tcaaacagtt ctatttacat tagtccaggc
    11221 tatgggtcag gttcgaagtc aagaacacgt tgaaatgctc caggaaatca ctcccacctt
    11281 gaaagaactg aaaacacaaa gtcagagtat ctataataat ttagtgagtt ttgcatcacc
    11341 cttagtcacc gatgcaacaa atgaatgttc gagtccaacg tcatctgcta cttatcagcc
    11401 atccttcgct gcagcagtcc ggagtaacac tggccagaag actcagcctg atgtcatgtc
    11461 acagaatgct agaaagctga tccagaaaaa tcttgctaca tcagctgata ctccaccaag
    11521 caccgttcca ggaactggca agagtgttgc ttgtagtcct aaaaaggcag tcagagaccc
    11581 taaaactggg aaagcggtgc aagagagaaa ctcctatgca gtgagtgtgt ggaagagagt
    11641 gaaagccaag ttagagggcc gagatgttga tccgaatagg aggatgtcag ttgctgaaca
    11701 ggttgactat gtcattaagg aagcaactaa tctagataac ttggctcagc tgtatgaagg
    11761 ttggacagcc tgggtgtgaa tggcaagaca gtagatgagt ctggttaagc gaggtcagac
    11821 atccaccaga atcaactcga cctcgactcg actctagagg atcccgggtg gcatccctgt
    11881 gacccctccc cagtgcctct cctggccctg gaagttgcca ctccagtgcc caccagcctt
    11941 gtcctaataa aattaagttg catcattttg tctgactagg tgtccttcta taatattatg
    12001 gggtggaggg gggtggtatg gagcaagggg caagttggga agacaacctg tagggcctgc
    12061 ggggtctatt gggaaccaag ctggagtgca gtggcacaat cttggctcac tgcaatctcc
    12121 gcctcctggg ttcaagcgat tctcctgcct cagcctcccg agttgttggg attccaggca
    12181 tgcatgacca ggctcagcta atttttgttt ttttggtaga gacggggttt caccatattg
    12241 gccaggctgg tctccaactc ctaatctcag gtgatctacc caccttggcc tcccaaattg
    12301 ctgggattac aggcgtgaac cactgctccc ttccctgtcc ttctgatttt aaaataacta
    12361 taccagcagg aggacgtcca gacacagcat aggctacctg gccatgccca accggtggga
    12421 catttgagtt gcttgcttgg cactgtcctc tcatgcgttg ggtccactca gtagatgcct
    12481 gttgaattgg gtacgcggcc agcttggctg tggaatgtgt gtcagttagg gtgtggaaag
    12541 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
    12601 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
    12661 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
    12721 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
    12781 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
    12841 tgcaaaaagc tcctcgagga actgaaaaac cagaaagtta attccctata gtgagtcgta
    12901 ttaaattcgt aatcatgtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
    12961 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
    13021 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
    13081 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
    13141 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
    13201 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
    13261 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
    13321 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
    13381 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
    13441 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
    13501 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
    13561 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
    13621 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
    13681 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
    13741 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
    13801 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
    13861 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
    13921 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
    13981 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
    14041 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
    14101 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
    14161 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
    14221 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
    14281 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
    14341 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
    14401 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
    14461 ggcgagttac atgatccccc atgttatgca aaaaagcggt tagctccttc ggtcctccga
    14521 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
    14581 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
    14641 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
    14701 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
    14761 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
    14821 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
    14881 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
    14941 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
    15001 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
    15061 tgccacctga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
    15121 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
    15181 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
    15241 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
    15301 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
    15361 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
    15421 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
    15481 aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttccatt cgccattcag
    15541 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc
    15601 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg
    15661 acgttgtaaa acgacggcca gtgccaagct gatctataca ttgaatcaat attggcaatt
    15721 agccatatta gtcattggtt atatagcata aatcaatatt gg
//
