LOCUS       sequence-391054-       15762 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses FLAG-tagged WT SMG1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15762)
  AUTHORS   McCann JJ, Fleenor DE, Chen J, Lai CH, Bass TE, Kastan MB
  TITLE     Participation of ATM, SMG1, and DDX5 in a DNA Damage-Induced 
            Alternative Splicing Pathway.
  JOURNAL   Radiat Res. 2023 Apr 1;199(4):406-421. doi: 10.1667/RADE-22-00219.1.
  PUBMED    36921295
REFERENCE   2  (bases 1 to 15762)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1667/RADE-22-00219"; journalName: "Radiat Res"; date: 
            "2023-04-1- 1"; volume: "199"; issue: "4"; pages: "406-421"
COMMENT     Sequence Label: FLAG SMG1 WT
FEATURES             Location/Qualifiers
     source          1..15762
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        102..481
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        482..685
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     636..656
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             719..742
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    6107..6118
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     polyA_signal    11887..12509
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(12086..12105)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(12533..12553)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        12538..12867
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      12718..12853
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(12905..12924)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(12906..12924)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(12938..12954)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12938..12954)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12951..12973)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12962..12978
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12986..12992,12993..13010,13011..13016))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13031..13052
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(13169..13186)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13340..13928)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13420..13439)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(14099..14890,14891..14959))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14722..14741
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14960..15064)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      15091..15546
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(15178..15197)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     15388..15409
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     15672..15694
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     15686..15703
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     15687..15703
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tatagcataa atcaatattg gctattggcc attgcatacg ttgtatctat atcataatat
       61 gtacatttat attggctcat gtccaatatg accgccatgt tgacattgat tattgactag
      121 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
      181 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
      241 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
      301 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
      361 tccgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
      421 gaccttacgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
      481 ggtgatgcgg ttttggcagt acaccaatgg gcgtggatag cggtttgact cacggggatt
      541 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
      601 ctttccaaaa tgtcgtaata accccgcccc gttgacgcaa atgggcggta ggcgtgtacg
      661 gtgggaggtc tatataagca gagctcgttt agtgaaccgt cagaattgat ctaccatgga
      721 ctacaaagac gatgacgaca agcttgcggc cgcgaattca tcgatagatc tgatatcggt
      781 accagtcgag atccatgtcg tgtcggggaa ggtaatgagc cgcagagccc cggggtctcg
      841 gctgagcagc ggcggcggcg gcggcggcac caagtatccg cggagctgga atgactggca
      901 acccagaact gatagtgcat cagccgaccc agataattta aaatattctt catccagaga
      961 tagaggtggt tcttcctctt acggactgca accttcaaat tcagctgtgg tgtctcggca
     1021 aaggcacgat gataccagag tccacgctga catacagaat gacgaaaagg gtggctacag
     1081 tgtcaatgga ggatctgggg aaaatactta tggtcggaag tcgttggggc aagagctgag
     1141 ggttaacaat gtgaccagcc ctgagttcac cagtgttcag catggcagtc gtgctttagc
     1201 caccaaagac atgaggaaat cacaggagag atcgatgtct tattctgatg agtctcgact
     1261 gtcgaatctt cttcggagga tcacccggga agacgacaga gaccgaagat tggctactgt
     1321 aaagcagttg aaagaattta ttcagcaacc agaaaataag ctggtactag ttaaacaatt
     1381 ggataatatc ttggctgctg tacatgacgt gcttaatgaa agtagcaaat tgcttcagga
     1441 gttgagacag gagggagctt gctgtcttgg ccttctttgt gcttctctga gctatgaggc
     1501 tgagaagatc ttcaagtgga tttttagcaa atttagctca tctgcaaaag atgaagttaa
     1561 actcctctac ttatgtgcca cctacaaagc actagagact gtaggagaaa agaaagcctt
     1621 ttcatctgta atgcagcttg taatgaccag cctgcagtct attcttgaaa atgtggatac
     1681 accagaattg ctttgtaaat gtgttaagtg cattcttttg gtggctcgat gttaccctca
     1741 tattttcagc actaatttta gggatacagt tgatatatta gttggatggc atatagatca
     1801 tactcagaaa ccttcgctca cgcagcaggt atctgggtgg ttgcagagtt tggagccatt
     1861 ttgggtagct gatcttgcat tttctactac tcttcttggt cagtttctgg aagacatgga
     1921 agcatatgct gaggacctca gccatgtggc ctctggggaa tcagtggatg aagatgtccc
     1981 tcctccatca gtgtcattac caaagctggc tgcacttctc cgggtattta gtactgtggt
     2041 gaggagcatt ggggaacgct tcagcccaat tcggggtcct ccaattactg aggcatatgt
     2101 aacagatgtt ctgtacagag taatgagatg tgtgacggct gcaaaccagg tgtttttttc
     2161 tgaggctgtg ttgacagctg ctaatgagtg tgttggtgtt ttgctcggca gcttggatcc
     2221 tagcatgact atacattgtg acatggtcat tacatatgga ttagaccaac tggagaattg
     2281 ccagacttgt ggtaccgatt atatcatctc agtcttgaat ttactcacgc tgattgttga
     2341 acagataaat acgaaactgc catcatcatt tgtagaaaaa ctgtttatac catcatctaa
     2401 actactattc ttgcgttatc ataaagaaaa agaggttgtt gctgtagccc atgctgttta
     2461 tcaagcagtg ctcagcttga agaatattcc tgttttggag actgcctata agttaatatt
     2521 gggagaaatg acttgtgccc taaacaacct cctacacagt ctacaacttc ctgaggcctg
     2581 ttctgaaata aaacatgagg cttttaagaa tcatgtgttc aatgtagaca atgcaaaatt
     2641 tgtagttata tttgacctca gtgccctgac tacaattgga aatgccaaaa actcactaat
     2701 agggatgtgg gcgctatctc caactgtctt tgcacttctg agtaagaatc tgatgattgt
     2761 gcacagtgac ctggctgttc acttccctgc cattcagtat gctgtgctct acacattgta
     2821 ttctcattgt accaggcatg atcactttat ctctagtagc ctcagttctt cctctccttc
     2881 tttgtttgat ggagctgtga ttagcactgt aactacggct acaaagaaac atttctcaat
     2941 tatattaaat cttctgggaa tattacttaa gaaagataac cttaaccagg acacgaggaa
     3001 actgttaatg acttgggctt tggaagcagc tgttttaatg aagaagtctg aaacatacgc
     3061 acctttattc tctcttccgt ctttccataa attttgcaaa ggccttttag ccaacactct
     3121 cgttgaagat gtgaatatct gtctgcaggc atgcagcagt ctacatgctc tgtcctcttc
     3181 cttgccagat gatcttttac agagatgtgt cgatgtttgc cgtgttcaac tagtgcacag
     3241 tggaactcgt attcgacaag catttggaaa actgttgaaa tcaattcctt tagatgttgt
     3301 cctaagcaat aacaatcaca cagaaattca agaaatttct ttagcattaa gaagtcacat
     3361 gagtaaagca ccaagtaata cattccaccc ccaagatttc tctgatgtta ttagttttat
     3421 tttgtatggg aactctcata gaacagggaa ggacaattgg ttggaaagac tgttctatag
     3481 ctgccagaga ctggataagc gtgaccagtc aacaattcca cgcaatctcc tgaagacaga
     3541 tgctgtcctt tggcagtggg ccatatggga agctgcacaa ttcactgttc tttctaagct
     3601 gagaacccca ctgggcagag ctcaagacac cttccagaca attgaaggta tcattcgaag
     3661 tctcgcagct cacacattaa accctgatca ggatgttagt cagtggacaa ctgcagacaa
     3721 tgatgaaggc catggtaaca accaacttag acttgttctt cttctgcagt atctggaaaa
     3781 tctggagaaa ttaatgtata atgcatacga gggatgtgct aatgcattaa cttcacctcc
     3841 caaggtcatt agaacttttt tctataccaa tcgccaaact tgtcaggact ggctaacgcg
     3901 gattcgactc tccatcatga gggtaggatt gttggcaggc cagcctgcag tgacagtgag
     3961 acatggcttt gacttgctta cagagatgaa aacaaccagc ctatctcagg ggaatgaatt
     4021 ggaagtaacc attatgatgg tggtagaagc attatgtgaa cttcattgtc ctgaagctat
     4081 acagggaatt gctgtctggt catcatctat tgttggaaaa aatcttctgt ggattaactc
     4141 agtggctcaa caggctgaag ggaggtttga aaaggcctct gtggagtacc aggaacacct
     4201 gtgtgccatg acaggtgttg attgctgcat ctccagcttt gacaaatcgg tgctcacctt
     4261 agccaatgct gggcgtaaca gtgccagccc gaaacattct ctgaatggtg aatccagaaa
     4321 aactgtgctg tccaaaccga ctgactcttc ccctgaggtt ataaattatt taggaaataa
     4381 agcatgtgag tgctacatct caattgccga ttgggctgct gtgcaggaat ggcagaacgc
     4441 tatccatgac ttgaaaaaga gtaccagtag cacttccctc aacctgaaag ctgacttcaa
     4501 ctatataaaa tcattaagca gctttgagtc tggaaaattt gttgaatgta ccgagcagtt
     4561 agaattgtta ccaggagaaa atatcaatct acttgctgga ggatcaaaag aaaaaataga
     4621 catgaaaaaa ctgcttccta acatgttaag tccggatccg agggaacttc agaaatccat
     4681 tgaagttcaa ttgttaagaa gttctgtttg tttggcaact gctttaaacc cgatagaaca
     4741 agatcagaag tggcagtcta taactgaaaa tgtggtaaag tacttgaagc aaacatcccg
     4801 catcgctatt ggacctctga gactttctac tttaacagtt tcacagtctt tgccagttct
     4861 aagtaccttg cagctgtatt gctcatctgc tttggagaac acagtttcta acagactttc
     4921 aacagaggac tgtcttattc cactcttcag tgaagcttta cgttcatgta aacagcatga
     4981 cgtgaggcca tggatgcagg cattaaggta tactatgtac cagaatcagt tgttggagaa
     5041 aattaaagaa caaacagtcc caattagaag ccatctcatg gaattaggtc taacagcagc
     5101 aaaatttgct agaaaacgag ggaatgtgtc ccttgcaaca agactgctgg cacagtgcag
     5161 tgaagttcag ctgggaaaga ccaccactgc acaggattta gtccaacatt ttaaaaaact
     5221 atcaacccaa ggtcaagtgg atgaaaaatg ggggcccgaa cttgatattg aaaaaaccaa
     5281 attgctttat acagcaggcc agtcaacaca tgcaatggaa atgttgagtt cttgtgccat
     5341 atctttctgc aagtctgtga aagctgaata tgcagttgct aaatcaattc tgacactggc
     5401 taaatggatc caggcagaat ggaaagagat ttcaggacag ctgaaacagg tttacagagc
     5461 tcagcaccaa cagaacttca caggtctttc tactttgtct aaaaacatac tcactctaat
     5521 agaactgcca tctgttaata cgatggaaga agagtatcct cggatcgaga gtgaatctac
     5581 agtgcatatt ggagttggag aacctgactt cattttggga cagttatatc acctgtcttc
     5641 agtacaggca cctgaagtag ccaaatcttg ggcagcgttg gccagctggg cttataggtg
     5701 gggcagaaag gtggttgaca atgccagtca gggagaaggt gttcgtctgc tgcctagaga
     5761 aaaatctgaa gttcagaatc tacttccaga cactataact gaggaagaga aagagagaat
     5821 atatggtatt cttggacagg ctgtgtgtcg gccggcgggg attcaggatg aagatataac
     5881 acttcagata actgagagtg aagacaacga agaagatgac atggttgatg ttatctggcg
     5941 tcagttgata tcaagctgcc catggctttc agaacttgat gaaagtgcaa ctgaaggagt
     6001 tattaaagtg tggaggaaag ttgtagatag aatattcagc ctgtacaaac tctcttgcag
     6061 tgcatacttt actttcctta aactcaacgc tggtcaaatt cctttagatg aggatgaccc
     6121 taggctgcat ttaagtcaca gagtggaaca gagcactgat gacatgattg tgatggccac
     6181 attgcgcctg ctgcggttgc tcgtgaagca tgctggtgag cttcggcagt atctggagca
     6241 cggcttggag acaacaccca ctgcaccatg gaggggaatt attccgcaac ttttctcacg
     6301 cttaaaccac cctgaagtgt atgtgcgcca aagtatttgt aaccttctct gccgtgtggc
     6361 tcaagattcc ccacatctca tattgtatcc tgcaatagtg ggtaccatat cgcttagtag
     6421 tgaatcccag gcttcaggaa ataaattttc cactgcaatt ccaactttac ttggcaatat
     6481 tcaaggagaa gaattgctgg tttctgaatg tgagggagga agtcctcctg catctcagga
     6541 tagcaataag gatgaaccta aaagtggatt aaatgaagac caagccatga tgcaggattg
     6601 ttacagcaaa attgtagata agctgtcctc tgcaaacccc accatggtat tacaggttca
     6661 gatgctcgtg gctgaactgc gcagggtcac tgtgctctgg gatgagctct ggctgggagt
     6721 tttgctgcaa caacacatgt atgtcctgag acgaattcag cagcttgaag atgaggtgaa
     6781 gagagtccag aacaacaaca ccttacgcaa agaagagaaa attgcaatca tgagggagaa
     6841 gcacacagct ttgatgaagc ccatcgtatt tgctttggag catgtgagga gtatcacagc
     6901 ggctcctgca gaaacacctc atgaaaaatg gtttcaggat aactatggtg atgccattga
     6961 aaatgcccta gaaaaactga agactccatt gaaccctgca aagcctggga gcagctggat
     7021 tccatttaaa gagataatgc taagtttgca acagagagca cagaaacgtg caagttacat
     7081 cttgcgtctt gaagaaatca gtccatggtt ggctgccatg actaacactg aaattgctct
     7141 tcctggggaa gtctcagcca gagacactgt cacaatccat agtgtgggcg gaaccatcac
     7201 aatcttaccg actaaaacca agccaaagaa acttctcttt cttggatcag atgggaagag
     7261 ctatccttat cttttcaaag gactggagga tttacatctg gatgagagaa taatgcagtt
     7321 cctatctatt gtgaatacca tgtttgctac aattaatcgc caagaaacac cccggttcca
     7381 tgctcgacac tattctgtaa caccactagg aacaagatca ggactaatcc agtgggtaga
     7441 tggagccaca cccttatttg gtctttacaa acgatggcaa caacgggaag ctgccttaca
     7501 agcacaaaag gcccaagatt cctaccaaac tcctcagaat cctggaattg taccccgtcc
     7561 tagtgaactt tattacagta aaattggccc tgctttgaaa acagttgggc ttagcctgga
     7621 tgtgtcccgt cgggattggc ctcttcatgt aatgaaggca gtattggaag agttaatgga
     7681 ggccacaccc ccgaatctcc ttgccaaaga gctctggtca tcttgcacaa cacctgatga
     7741 atggtggaga gttacgcagt cttatgcaag atctactgca gtcatgtcta tggttggata
     7801 cataattggc cttggagaca gacatctgga taatgttctt atagatatga cgactggaga
     7861 agttgttcac atagattaca atgtttgctt tgaaaaaggt aaaagcctta gagttcctga
     7921 gaaagtacct tttcgaatga cacaaaacat tgaaacagca ctgggtgtaa ctggagtaga
     7981 aggtgtattt aggctttcat gtgagcaggt tttacacatt atgcggcgtg gcagagagac
     8041 cctgctgacg ctgctggagg cctttgtgta cgaccctctg gtggactgga cagcaggagg
     8101 cgaggctggg tttgctggtg ctgtctatgg tggaggtggc cagcaggccg agagcaagca
     8161 gagcaagaga gagatggagc gagagatcac ccgcagcctg ttttcttcta gagtagctga
     8221 gattaaggtg aactggttta agaatagaga tgagatgctg gttgtgcttc ccaagttgga
     8281 cggtagctta gatgaatacc taagcttgca agagcaactg acagatgtgg aaaaactgca
     8341 gggcaaacta ctggaggaaa tagagtttct agaaggagct gaaggggtgg atcatccttc
     8401 tcatactctg caacacaggt attctgagca cacccaacta cagactcagc aaagagctgt
     8461 tcaggaagca atccaggtga agctgaatga atttgaacaa tggataacac attatcaggc
     8521 tgcattcaat aatttagaag caacacagct tgcaagcttg cttcaagaga taagcacaca
     8581 aatggacctt ggtcctccaa gttacgtgcc agcaacagcc tttctgcaga atgctggtca
     8641 ggcccacttg attagccagt gcgagcagct ggagggggag gttggtgctc tcctgcagca
     8701 gaggcgctcc gtgctccgtg gctgtctgga gcaactgcat cactatgcaa ccgtggccct
     8761 gcagtatccg aaggccatat ttcagaaaca tcgaattgaa cagtggaaga cctggatgga
     8821 agagctcatc tgtaacacca cagtagagcg ttgtcaagag ctctatagga aatatgaaat
     8881 gcaatatgct ccccagccac ccccaacagt gtgtcagttc atcactgcca ctgaaatgac
     8941 cctgcagcga tacgcagcag acatcaacag cagacttatt agacaagtgg aacgcttgaa
     9001 acaggaagct gtcactgtgc cagtttgtga agatcagttg aaagaaattg aacgttgcat
     9061 taaagttttc cttcatgaga atggagaaga aggatctttg agtctagcaa gtgttattat
     9121 ttctgccctt tgtaccctta caaggcgtaa cctgatgatg gaaggtgcag cgtcaagtgc
     9181 tggagaacag ctggttgatc tgacttctcg ggatggagcc tggttcttgg aggaactctg
     9241 cagtatgagc ggaaacgtca cctgcttggt tcagttactg aagcagtgcc acctggtgcc
     9301 acaggactta gatatcccga accccatgga agcgtctgag acagttcact tagccaatgg
     9361 agtgtatacc tcacttcagg aattgaattc gaatttccgg caaatcatat ttccagaagc
     9421 acttcgatgt ttaatgaaag gggaatacac gttagaaagt atgctgcatg aactggacgg
     9481 tcttattgag cagaccaccg atggcgttcc cctgcagact ctagtggaat ctcttcaggc
     9541 ctacttaaga aacgcagcta tgggactgga agaagaaaca catgctcatt acatcgatgt
     9601 tgccagacta ctacatgctc agtacggtga attaatccaa ccgagaaatg gttcagttga
     9661 tgaaacaccc aaaatgtcag ctggccagat gcttttggta gcattcgatg gcatgtttgc
     9721 tcaagttgaa actgctttca gcttattagt tgaaaagttg aacaagatgg aaattcccat
     9781 agcttggcga aagattgaca tcataaggga agccaggagt actcaagtta atttttttga
     9841 tgatgataat caccggcagg tgctagaaga gattttcttt ctaaaaagac tacagactat
     9901 taaggagttc ttcaggctct gtggtacctt ttctaaaaca ttgtcaggat caagttcact
     9961 tgaagatcag aatactgtga atgggcctgt acagattgtc aatgtgaaaa ccctttttag
    10021 aaactcttgt ttcagtgaag accaaatggc caaacctatc aaggcattca cagctgactt
    10081 tgtgaggcag ctcttgatag ggctacccaa ccaagccctc ggactcacac tgtgcagttt
    10141 tatcagtgct ctgggtgtag acatcattgc tcaagtagag gcaaaggact ttggtgccga
    10201 aagcaaagtt tctgttgatg atctctgtaa gaaagcggtg gaacataaca tccagatagg
    10261 gaagttctct cagctggtta tgaacagggc aactgtgtta gcaagttctt acgacactgc
    10321 ctggaagaag catgacttgg tgcgaaggct agaaaccagt atttcttctt gtaagacaag
    10381 cctgcagcgg gttcagctgc atattgccat gtttcagtgg caacatgaag atctacttat
    10441 caatagacca caagccatgt cagtcacacc tcccccacgg tctgctatcc taaccagcat
    10501 gaaaaagaag ctgcataccc tgagccagat tgaaacttct attgcgacag ttcaggagaa
    10561 gctagctgca cttgaatcaa gtattgaaca gcgactcaag tgggcaggtg gtgccaaccc
    10621 tgcattggcc cctgtactac aagattttga agcaacgata gctgaaagaa gaaatcttgt
    10681 ccttaaagag agccaaagag caagtcaggt cacatttctc tgcagcaata tcattcattt
    10741 tgaaagttta cgaacaagaa ctgcagaagc cttaaacctg gatgcggcgt tatttgaact
    10801 aatcaagcga tgtcagcaga tgtgttcgtt tgcatcacag tttaacagtt cagtgtctga
    10861 gttagagctt cgtttattac agagagtgga cactggtctt gaacatccta ttggcagctc
    10921 tgaatggctt ttgtcagcac acaaacagtt gacccaggat atgtctactc agagggcaat
    10981 tcagacagag aaagagcagc agatagaaac ggtctgtgaa acaattcaga atctggttga
    11041 taatataaag actgtgctca ctggtcataa ccgacagctt ggagatgtca aacatctctt
    11101 gaaagctatg gctaaggatg aagaagctgc tctggcagat ggtgaagatg ttccctatga
    11161 gaacagtgtt aggcagtttt tgggtgaata taaatcatgg caagacaaca ttcaaacagt
    11221 tctatttaca ttagtccagg ctatgggtca ggttcgaagt caagaacacg ttgaaatgct
    11281 ccaggaaatc actcccacct tgaaagaact gaaaacacaa agtcagagta tctataataa
    11341 tttagtgagt tttgcatcac ccttagtcac cgatgcaaca aatgaatgtt cgagtccaac
    11401 gtcatctgct acttatcagc catccttcgc tgcagcagtc cggagtaaca ctggccagaa
    11461 gactcagcct gatgtcatgt cacagaatgc tagaaagctg atccagaaaa atcttgctac
    11521 atcagctgat actccaccaa gcaccgttcc aggaactggc aagagtgttg cttgtagtcc
    11581 taaaaaggca gtcagagacc ctaaaactgg gaaagcggtg caagagagaa actcctatgc
    11641 agtgagtgtg tggaagagag tgaaagccaa gttagagggc cgagatgttg atccgaatag
    11701 gaggatgtca gttgctgaac aggttgacta tgtcattaag gaagcaacta atctagataa
    11761 cttggctcag ctgtatgaag gttggacagc ctgggtgtga atggcaagac agtagatgag
    11821 tctggttaag cgaggtcaga catccaccag aatcaactcg acctcgactc gactctagag
    11881 gatcccgggt ggcatccctg tgacccctcc ccagtgcctc tcctggccct ggaagttgcc
    11941 actccagtgc ccaccagcct tgtcctaata aaattaagtt gcatcatttt gtctgactag
    12001 gtgtccttct ataatattat ggggtggagg ggggtggtat ggagcaaggg gcaagttggg
    12061 aagacaacct gtagggcctg cggggtctat tgggaaccaa gctggagtgc agtggcacaa
    12121 tcttggctca ctgcaatctc cgcctcctgg gttcaagcga ttctcctgcc tcagcctccc
    12181 gagttgttgg gattccaggc atgcatgacc aggctcagct aatttttgtt tttttggtag
    12241 agacggggtt tcaccatatt ggccaggctg gtctccaact cctaatctca ggtgatctac
    12301 ccaccttggc ctcccaaatt gctgggatta caggcgtgaa ccactgctcc cttccctgtc
    12361 cttctgattt taaaataact ataccagcag gaggacgtcc agacacagca taggctacct
    12421 ggccatgccc aaccggtggg acatttgagt tgcttgcttg gcactgtcct ctcatgcgtt
    12481 gggtccactc agtagatgcc tgttgaattg ggtacgcggc cagcttggct gtggaatgtg
    12541 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
    12601 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
    12661 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
    12721 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
    12781 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
    12841 ttttttggag gcctaggctt ttgcaaaaag ctcctcgagg aactgaaaaa ccagaaagtt
    12901 aattccctat agtgagtcgt attaaattcg taatcatgtc atagctgttt cctgtgtgaa
    12961 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
    13021 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
    13081 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
    13141 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
    13201 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
    13261 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
    13321 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
    13381 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
    13441 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
    13501 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
    13561 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
    13621 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
    13681 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
    13741 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
    13801 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
    13861 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
    13921 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
    13981 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
    14041 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
    14101 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
    14161 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
    14221 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
    14281 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
    14341 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
    14401 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
    14461 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttatgc aaaaaagcgg
    14521 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
    14581 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
    14641 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
    14701 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
    14761 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
    14821 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
    14881 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
    14941 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
    15001 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
    15061 cgcgcacatt tccccgaaaa gtgccacctg acgcgccctg tagcggcgca ttaagcgcgg
    15121 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
    15181 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
    15241 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
    15301 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
    15361 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
    15421 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
    15481 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta
    15541 caatttccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc
    15601 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac
    15661 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgccaagc tgatctatac
    15721 attgaatcaa tattggcaat tagccatatt agtcattggt ta
//
